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  • Mercredi 18 avril 11:30-13:00 - Pierre Gonczy - Ecole Polytechnique Fédérale de Lausanne, Suisse

    Mechanisms of centriole assembly

    Résumé : The centriole is a remarkable microtubule-based organelle that is essential for the formation of cilia, flagella and centrosomes. The centriole is organized around a nine-fold symmetrical cartwheel typically 100 nm in height, which is critical for the onset of organelle biogenesis. The cartwheel comprises a stack of ring-containing entities that each accommodates nine homodimers of SAS-6 proteins. In contrast to the knowledge about the self-assembly properties of SAS-6 proteins, the mechanisms enabling ring stacking are poorly understood. Furthermore, the assembly dynamics of SAS-6 ring-containing entities remains elusive.
    After introducing the subject matter, I will report notably on our development of a cell-free assay to address this important open question using the Chlamydomonas reinhardtii SAS-6 protein CrSAS-6. Using high-speed atomic force microscopy (AFM), we monitored the assembly dynamics of CrSAS-6 homodimers, and thus determined possible routes and kinetic rates for CrSAS-6 ring formation.

    Invité par Anne-Marie Tassin, équipe Biogénese et fonction des structures centriolaires et ciliaires

    Lieu : Auditorium I2BC - Bâtiment 21
    Campus CNRS de Gif-sur-Yvette

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