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Accueil > Départements > Biologie des Génomes > Carmela GIGLIONE : Maturation des protéines, destinée cellulaire et thérapeutique

Evidence for in vivo N-terminal maturation of proteins synthesized in plastids

This data should be quoted as part of the review paper by Giglione & Meinnel (2001) Trends Plant Sci. 6(12), 566-572

If some data or information is missing or erroneous, please feel free to email your corrections to Thierry Meinnel so that this table can be up-dated immediately.

I- N-terminal sequence of proteins determined experimentally (59 different proteins)

(o) Proteins retaining the N-formyl (7 occurrences)

(i) Proteins undergoing N-formyl cleavage only (18 occurrences)

(ii) Proteins undergoing N-formyl cleavage and N-methionine cleavage (33 occurrences)

(iii) Proteins undergoing N-formylmethionine-serine cleavage (2 occurrences)

(iv) Proteins undergoing extensive cleavages (3 occurrences)

II- Proposed cleavages for proteins with undetermined N-terminus

(21 different proteins)

(o) Proteins retaining the N-formyl


Gene name a


Protein name and function a


Plant
b


N-terminal modifications and cleavages c


References
d

atpH

ATP synthase c chain (sub-unit
III)

C, P, S

f-Met-Asn

1-3

psaI

PSI sub-unit VIII

B

f-Met-Thr ; see also (i)

4

psaJ

PSI sub-unit IX

P, S

f-Met-Arg

5, 6

psbI

PSII I protein

P, S

f-Met-Leu

7-9, 47

psbM

PSII M reaction centre
protein

P

f-Met-Glu

47

psbT

PSII T protein

P, S

f-Met-Glu

10

ycf9

PsbZ protein. Assembly and/or maintenance of stable PSII light harvesting complex

P

f-Met-Thr

47

(i) Proteins undergoing N-formyl cleavage only


Gene name a


Protein name and function a


Plant
b


N-terminal modifications and cleavages c


References
d

atpB

ATP synthase ß chain

R

f>Met-Arg(NB partial retention of the
N-formyl was observed), see also (ii)

11

ndhA

NADH dehydrogenase chain
1

P

f>Met-Ile

12

ndhI

NADH dehydrogenase sub-unit I
(frxb protein)

T

f>Met-Ile

46

petG

Cytochrome
b6f complex sub-unit V

C
S

f>Met-Val
f>Met-Ile

13
13

petL

Cytochrome
b6f complex sub-unit VI

C

f>Met-Leu

48

petN or ycf6

Cytochrome
b6f complex sub-unit VIII

T

f>Met-Asp

14

psaB

PSI P700 apoprotein A2

Ma

f>Met-Glu

15

psaI

PSI sub-unit VIII

P
S

f>Met-Ile, see also (o)
f>Met-Asn

5
6

psbG or ndhK

PSII sub-unit K

P

f>Met-Asn

12

rpl14

Ribosomal protein L14

S

f>Met-Ile

16

rpl16

Ribosomal protein L16

S

f>Met-Leu

16

rpl23

Ribosomal protein L23

T

f>Met-Asp

17

rpl36

Ribosomal protein L36

S

f>Met-Lys

16

rpoA

RNA polymerase alpha
sub-unit

Ma

f>Met-Val

18

rpoB

RNA polymerase ß
sub-unit

Ma

f>Met-Leu, <FONT
size="2">see also (ii)

18

rps15

Ribosomal protein S15

S

f>Met-Lys*

19

rps16
Ribosomal protein S16
S
f>Met-Val*
19

rps18

Ribosomal protein S18

S

f>Met-Asp

19

(ii) Proteins undergoing N-formyl cleavage and
N-methionine cleavage


Gene

name a


Protein name and function a


Plant

b


N-terminal modifications and cleavages c


References

d

atpA

ATP synthase alpha chain

C

fMet>Ala

3

atpB

ATP synthase ß chain

C

fMet>Ser, see also (i)

3, 20

atpE

ATP synthase epsilon
chain

C, W

fMet>Ser

21

clpP

ATP-dependent protease

A

fMet>Pro

22

petB

Cytochome
b6

S

fMet>Ser

23

petD

Cytochrome
b6f sub-unit IV

S

fMet>Gly

23

psaC

PSI 9 kDa protein

A, B, Cu, P, R, S, T

fMet>Ser

5, 11, 24-27

psbA

PSII protein D1 (32 kDa)

P, S

fMet>ThrP+

28, 29, 47

psbB

PSII P680 chlorophyll A
apoprotein (CP47 protein)

P

fMet>Gly+

47

psbD

PSII protein D2

A, P, S

fMet>ThrP+

28-30, 47

psbE

PSII cytochrome b559 alpha
chain

C
P, S

fMet>Ala
fMet>Ser

31
8, 9, 32

psbF

PSII cytochrome b559 ß
chain

P, S

fMet>Thr+

8, 9, 33

psbH

PSII 10kDa
phosphoprotein

A, C, P, S, W

fMet>Ala-ThrP

30, 34-37, 47

psbJ

PII reaction centre J
protein

P

fMet>Ala+

47

psbL

PSII reaction centre L protein
(5 kDa)

P, S

fMet>Thr

10, 38, 47

rbcL

Rubisco large sub-unit

A

fMet>Ser+ ; see also (iii)

11

rpoB

RNA polymerase ß
sub-unit

Mu

fMet>Gly,
see also
(i)

39

rpoC1

RNA polymerase ß1
sub-unit

Ma

fMet>Ile

40

rpoC2

RNA polymerase ß2
sub-unit

Ma, Mu

fMet>Ala

18, 39

rpl2

Ribosomal protein L2

S, T

fMet>Ala*

16, 41

rpl20

Ribosomal protein L20

S

fMet>Thr

16

rpl22

Ribosomal protein L22

S

fMet>Gly

16

rpl32

Ribosomal protein L32

S

fMet>Ala

16

rpl33

Ribosomal protein L33

S

fMet>Ala

16

rps2

Ribosomal protein S2

S

fMet>Thr

19

rps3

Ribosomal protein S3

S

fMet>Gly*

19

rps4

Ribosomal protein S4

S

fMet>Ser

19

rps7

Ribosomal protein S7

S

fMet>Ser*

19

rps8

Ribosomal protein S8

S

fMet>Gly*

19

rps11

Ribosomal protein S11

S

fMet>Ala

19

rps12

Ribosomal protein S12

S

fMet>Pro*

19

rps14

Ribosomal protein S14

S

fMet>Ala*

19

rps19

Ribosomal protein S19

S

fMet>Thr*

19

(iii) Proteins undergoing N-formylmethionine-X
cleavage


Gene name a


Protein name and function a


Plant

b


N-terminal modifications and cleavages c


References
d

psbC

PSII 44 kDa P6 protein (CP43)

A, P, S, T

fMet-Glu>ThrP+

28, 30, 47, 49

rbcL

Rubisco large sub-unit

Me, S, T, W

fMet-Ser>Pro+* ; see also
(ii)

42

(iv) Proteins undergoing extensive cleavages

 


Gene name a


Protein name and function a


Plant
b


N-terminal modifications and cleavages c


References
d

atpI

ATP synthase a chain (sub-unit
IV)

C, S

d(fMet1-Ser18)>Gly

3, 43

petA

Apocytochrome f
precursor

C, P

d(fMet1-Ala35)>Tyr

44, 45

psbk

PSII 4kDa protein

S

d(fMet1-Gly22)>Lys

10, 38

Proposed cleavages for proteins with undetermined N-terminus

Gene name a

Protein name and function a

N-terminus as deduced from nucleotide sequencea

N-terminal cleavage proposed (hypothetical)

accD

Acetyl-CoA carboxylase carboxyl
transferase sub-unit b

MEKSW

f>Met-Glu

atpF

ATP synthase b chain (sub-unit
I)

MKNLT

f>Met-Lys

cemA
or ycf10

Integral membrane protein. May be involved in proton extrusion, indirectly promotes inorganic carbon uptake into chloroplasts

MAKKK

fMet>Ala

matK

Group II introns
maturase

MCHFR

fMet>Cys

ndhB

NADH dehydrogenase sub-unit
2

MIWHV

f>Met-Ile

ndhC

NADH dehydrogenase sub-unit
3

MFLLY

f>Met-Phe

ndhD

NADH dehydrogenase sub-unit
4

MYLVF

f>Met-Tyr

ndhE

NADH dehydrogenase sub-unit
4L

MILEH

f>Met-Ile

ndhF

NADH dehydrogenase sub-unit
5

MEHTY

f>Met-Glu

ndhG

NADH dehydrogenase sub-unit
6

MDLPG

f>Met-Asp

ndhH

NADH dehydrogenase 49 kDa

MKRPV

f>Met-Lys

ndhJ

NADH dehydrogenase sub-unit
J

MQGTC
f>Met-Gln

psaA

PSI P700 apoprotein A1

MIIRSP

f>Met-Ile

psbN

PSII N reaction centre
protein

METAT

f>Met-Glu

ycf1

Essential gene for cell survival, unknown function

MVFQS

f>Met-Val or <FONT
size="2">f-Met>Val

ycf2

Essential gene for cell survival, unknown function

MKGHQ

f>Met-Lys

ycf3

Stable accumulation of PSI
complex

MSAQS

fMet>Ser

ycf4

Stable accumulation of PSI
complex

MSWRS

fMet>Ser

ycf5

PSI maintenance

MIFSIL

f>Met-Ile

ycf7
Orf31, unknown function
MPTIT
fMet>Pro

ycf15

Orf77 ; 9 kDa protein, unknown function

MLLLK

f>Met-Leu


a The genes encoding the 79 different plastid-encoded proteins of A. thaliana are described
(see Sato et al. (1999) Complete structure of the chloroplast genome of Arabidopsis thaliana).
Additional genes found in other sequenced plastid genomes of higher plants are also indicated (see data of complete plastid genome sequences).
The function of the protein is indicated when known.

b A, Arabidopsis thaliana ; B, barley ; C, Chlamydomonas reinhardtii ; Cu, cucumber ; Ma, maize ; Me, muskmelon ;
Mu mustard ; P, pea ; R, rice ; S, spinach ; T, tobacco ; W, wheat.

c N-terminal sequence of the corresponding proteins is indicated.
The final deduced cleavage site is indicated by >, representing cleavage by PDF (f>Met) or by PDF plus MAP (fMet>)
or by further peptidases if the extent of the cleavage is larger (d is the corresponding deletion).
f is N-formyl.
The presence of a post-translational modification, such as N-methylation(*), N-acetylation(+) and/or threonine N-phosphorylation (ThrP) is reported.

d references

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