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Accueil > Départements > Biologie des Génomes > Joël ACKER & Olivier LEFEBVRE : Régulation Nucléaire et Stress

Publications de l’équipe

2015


  • N. - T. - T. Nguyen, C. Saguez, C. Conesa, O. Lefebvre, et J. Acker, « Identification of proteins associated with RNA polymerase III using a modified tandem chromatin affinity purification », Gene, vol. 556, nᵒ 1, p. 51-60, 2015.
    Résumé : To identify the proteins associated with the RNA polymerase III (Pol III) machinery in exponentially growing yeast cells, we developed our own tandem chromatin affinity purification procedure (TChAP) after in vivo cross-link, allowing a reproducible and good recovery of the protein bait and its associated partners. In contrast to TFIIIA that could only be purified as a free protein, this protocol allows us to capture free Pol III together with Pol III bound on its target genes. Transcription factors, elongation factors, RNA-associated proteins and proteins involved in Pol III biogenesis were identified by mass spectrometry. Interestingly, the presence of all the TFIIIB subunits found associated with Pol III together with the absence of TFIIIC and chromatin factors including histones suggest that DNA-bound Pol III purified using TChAP is mainly engaged in transcription reinitiation.
    Mots-clés : Chromatin, Chromatography, Affinity, Cross-Linking Reagents, DBG, Gene Regulatory Networks, Mass Spectrometry, Protein Binding, Protein–protein interaction, RNA Polymerase III, RNS, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TAP-MS, TFIIIA, Transcription Factors, Transcription Initiation, Genetic.
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Publications Principales avant 2015

  • Acker*, J., Nguyen, N.T.T., Vandamme, M., Tavenet, A., Briand-Suleau, A., Conesa*, C., (2014). Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle. PLoS ONE 9, e114587. [PubMed] [PDF]
  • Acker, J., Conesa, C., Lefebvre*, O., (2013). Yeast RNA polymerase III transcription factors and effectors. Biochim Biophys Acta 1829, 283–295. [PubMed] [Article]
  • Morawiec, E., Wichtowska, D., Graczyk, D., Conesa, C., Lefebvre, O., Boguta*, M., (2013). Maf1, repressor of tRNA transcription, is involved in the control of gluconeogenetic genes in Saccharomyces cerevisiae. Gene 526, 16–22. [PubMed] [Article]
  • Graczyk, D., Debski, J., Muszynska, G., Bretner, M., Lefebvre, O., Boguta*, M., (2011). Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation. Proc Natl Acad Sci USA 108, 4926–4931. [PubMed] [PDF]
  • Conesa*, C., Acker*, J., (2010). Sub1/PC4 a chromatin associated protein with multiple functions in transcription. RNA Biol 7, 287–290. [PubMed] [PDF]
  • Gajda, A., Towpik, J., Steuerwald, U., Müller, C.W., Lefebvre*, O., Boguta*, M., (2010). Full repression of RNA polymerase III transcription requires interaction between two domains of its negative regulator Maf1. J Biol Chem 285, 35719–35727. [PubMed] [PDF]
  • Tavenet, A., Suleau, A., Dubreuil, G., Ferrari, R., Ducrot, C., Michaut, M., Aude, J.C., Dieci, G., Lefebvre, O., Conesa*, C., Acker*, J., (2009). Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription. Proc Natl Acad Sci USA 106, 14265–14270. [PubMed] [PDF]
  • Acker, J., Ozanne, C., Kachouri-Lafond, R., Gaillardin, C., Neuvéglise, C., and Marck*, C. (2008). Dicistronic tRNA-5S rRNA genes in Yarrowia lipolytica : an alternative TFIIIA-independent way for expression of 5S rRNA genes. Nucleic Acids Res 36, 5832–5844. [PubMed] [PDF]
  • Ducrot, C., Lefebvre, O., Landrieux, E., Guirouilh-Barbat, J., Sentenac, A., Acker*, J. (2006) Reconstitution of the yeast RNA polymerase III transcription system with all recombinant factors. J Biol Chem 281, 11685-11689. [PubMed] [PDF]
  • Oficjalska-Pham, D., Harismendy, O., Smagowicz, W.J., Gonzalez de Peredo, A., Boguta, M., Sentenac, A., Lefebvre*, O. (2006) General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1. Mol Cell 22, 623-632. [PubMed] [Article]
  • Mylona, A., Fernández-Tornero, C., Legrand, P., Haupt, M., Sentenac, A., Acker*, J., Müller*, C.W. (2006) Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC : insights into preinitiation complex assembly. Mol Cell 24, 221-232. [PubMed] [Article]
  • Harismendy, O., Gendrel C.G., Soularue, P., Gidrol, X., Sentenac, A., Werner, M., Lefebvre*, O.
    (2003) Genome-wide location of yeast RNA polymerase III transcription machinery. EMBO J 22, 4738-4747. [PubMed] [PDF]

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