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Accueil > Départements > Biochimie, Biophysique et Biologie Structurale > Niko HILDEBRANDT : NanoBioPhotonique


Denn was man schwarz auf weiß besitzt, kann man getrost nach Hause tragen. (Goethe)
What we possess in black and white, we can in peace and comfort bear away.

Reviews and Perspectives

1. O. Tagit and N. Hildebrandt. Fluorescence Sensing of Circulating Diagnostic Biomarkers Using Molecular Probes and Nanoparticles. ACS Sensors 2017, 2 (1), 31-45.

2. N. Hildebrandt, C. M. Spillmann, W. R. Algar, T. Pons, M. H. Stewart, E. Oh, K. Susumu, S. A. Díaz, J. B. Delehanty, and I. L. Medintz. Energy Transfer with Semiconductor Quantum Dot Bioconjugates : A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications. Chemical Reviews 2017, 117 (2), 536-711.

3. M. Sy, A. Nonat, N. Hildebrandt, and L.J. Charbonnière. Lanthanide-based luminescent biolabelling. Chemical Communications 2016, 52, 5080-5095.

4. M. Cardoso Dos Santos and N. Hildebrandt. Recent Developments in Lanthanide-to-Quantum Dot FRET Using Time-Gated Fluorescence Detection and Photon Upconversion. TrAC – Trends in Analytical Chemistry 2016, 84, 60-71.

5. D. Geißler and N. Hildebrandt. Recent developments in FRET diagnostics using quantum dots. Analytical and Bioanalytical Chemistry 2016, 408 (17), 4475-4483.

6. K.D. Wegner and N. Hildebrandt. Quantum Dots : Bright and Versatile In vitro and In vivo Fluorescence Imaging Biosensors. Chemical Society Reviews 2015, 44, 4792-4834.

7. N. Hildebrandt, K. D. Wegner, and W. R. Algar. Luminescent Terbium Complexes : Superior Förster Resonance Energy Transfer Donors for Flexible and Sensitive Multiplexed Biosensing. Coordination Chemistry Reviews 2014, 273–274, 125–138.

8. D. Geißler, S. Lindén, K. Liermann, K. D. Wegner, L. J. Charbonnière, and N. Hildebrandt. Lanthanides and Quantum Dots as Förster Resonance Energy Transfer Agents for Diagnostics and Cellular Imaging. Inorganic Chemistry 2014, 53, 1824-1838.

9. W. R. Algar, H. Kim, I. L. Medintz, and N. Hildebrandt. Emerging non-traditional Förster resonance energy transfer configurations with semiconductor quantum dots : Investigations and applications. Coordination Chemistry Reviews 2014, 263-264, 65-85.

10. B. Hötzer, I.L. Medintz, N. Hildebrandt. Fluorescence in Nanobiotechnology – Sophisticated Fluorophores for Novel Applications. Small 2012, 8 (15), 2297-2326.

11. Z. Jin, N. Hildebrandt. Quantum dots for in vitro diagnostics and cellular imaging. Trends in Biotechnology 2012, 30 (7), 394-403.

12. N. Hildebrandt. Biofunctional Quantum Dots : Controlled Conjugation for Multiplexed Biosensors. ACS Nano 2011, 5(7), 5286–5290.

13. D. Geißler, N. Hildebrandt. Lanthanide Complexes in FRET Applications. Current Inorganic Chemistry 2011, 1, 17-35.

14. L.J. Charbonnière, N. Hildebrandt. Lanthanide Complexes and Quantum Dots : A Bright Wedding for Resonance Energy Transfer. European Journal of Inorganic Chemistry 2008, 3241–3251.

15. N. Hildebrandt, H.-G. Löhmannsröben. Quantum Dot Nanocrystals and Supramolecular Lanthanide Complexes - Energy Transfer Systems for Sensitive In Vitro Diagnostics and High Throughput Screening in Chemical Biology. Current Chemical Biology 2007 ; 1(2) : 167-186.

Research papers

16. X. Qiu, J. Guo, Z. Jin, I. L. Medintz, N. Hildebrandt. Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning. Small 2017, DOI : 10.1002/smll.201700332.

17. M. Amjadi, T. Hallaj, H. Asadollahi, Z. Song, M. de Frutos, and N. Hildebrandt. Facile synthesis of carbon quantum dot/silver nanocomposite and its application for colorimetric detection of methimazole. Sensors and Actuators B : Chemical 2017, 244, 425-432.

18. S. Bhuckory, L. Mattera, K. D. Wegner, X. Qiu, Y-T. Wu, L. J. Charbonnière, P. Reiss, and N. Hildebrandt. Direct conjugation of antibodies to the ZnS shell of quantum dots for FRET immunoassays with low picomolar detection limits. Chemical Communications 2016, 52, 14423-14425.

19. X. Qiu, K. D. Wegner, Y.-T. Wu, P.M.P. van Bergen en Henegouwen, T. L. Jennings, and N. Hildebrandt. Nanobodies and Antibodies for Duplexed EGFR/HER2 Immunoassays Using Terbium-to-Quantum Dot FRET. Chemistry of Materials 2016, 28, 8256-8267.

20. H. S. Afsari, M. Cardoso dos Santos, S. Lindén, T. Chen, X. Qiu, P. M. P. van Bergen en Henegouwen, T. L. Jennings, K. Susumu, I. L. Medintz, N. Hildebrandt, L. W. Miller, Time-gated FRET nanoassemblies for rapid and sensitive intra- and extracellular fluorescence imaging. Science Advances 2016, 2, e1600265.

21. L. Mattera, S. Bhuckory, K.D. Wegner, X. Qiu, F. Agnese, C. Lincheneau, T. Senden, D. Djurado, L.J. Charbonnière, N. Hildebrandt, and P. Reiss. Compact quantum dot-antibody conjugates for FRET immunoassays with subnanomolar detection limits. Nanoscale 2016, 8, 11275-11283.

22. A. Yahia Ammar, D. Sierra, F. Mérola, N. Hildebrandt, and X. Le Guével. Self-Assembled Gold Nanoclusters For Bright Fluorescence Imaging and Enhanced Drug Delivery. ACS Nano 2016, 10 (2), 2591-2599.

23. S. Bhuckory, O. Lefebvre, X. Qiu, K. D. Wegner, and N. Hildebrandt. Evaluating quantum dot performance in homogeneous FRET immunoassays for prostate specific antigen. Sensors 2016, 16(2), 197 (11 pages).

24. X. Qiu and N. Hildebrandt. Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer. ACS Nano 2015, 9 (8), 8449-8457.

25. Z. Jin, D. Geißler, X. Qiu, K. D. Wegner, and N. Hildebrandt. Rapid, Amplification-Free, and Sensitive Diagnostic Assay for Single-Step Multiplexed Fluorescence Detection of MicroRNA. Angewandte Chemie International Edition 2015, 54, 10024-10029.

26. O. Tagit, G. Annio, and N. Hildebrandt. Terbium to quantum rod Förster resonance energy transfer for homogeneous bioassays with picomolar detection limits. Microchimica Acta 2015, 182 (9), 1693-1700.

27. A. Gaudin, O. Tagit, D. Sobot, S. Lepetre-Mouelhi, J. Mougin, T. F. Martens, K. Braeckmans, V. Nicolas, D. Desmaële, S. C. de Smedt, N. Hildebrandt, P. Couvreur, K. Andrieux. Transport Mechanisms of Squalenoyl Adenosine Nanoparticles Across the Blood-Brain Barrier. Chemistry of Materials 2015, 27 (10), 3636–3647.

28. L. Mattsson, K. D. Wegner, N. Hildebrandt, and T. Soukka. Upconverting Nanoparticle to Quantum Dot FRET for Homogeneous Double-Nano Biosensors. RSC Advances 2015, 5, 13270-13277.

29. S. Lindén, M. K. Singh, K. D. Wegner, M. Regairaz, F. Dautry, F. Treussart, and N. Hildebrandt. Terbium-Based Time-Gated Förster Resonance Energy Transfer Imaging for Evaluating Protein-Protein Interactions on Cell Membranes. Dalton Transactions 2015, 44, 4994-5003.

30. A. Gaudin, M. Yemisci, H. Eroglu, S. Lepetre-Mouelhi, O. F. Turkoglu, B. Dönmez-Demir, S. Caban, M. F. Sargon, S. Garcia-Argote, G. Pieters, O. Loreau, B. Rousseau, O. Tagit, N. Hildebrandt, Y. Le Dantec, J. Mougin, S. Valetti, H. Chacun, V. Nicolas, D. Desmaële, K. Andrieux, Y. Capan, T. Dalkara, and P. Couvreur. Squalenoyl adenosine nanoparticles provide neuroprotection after stroke and spinal cord injury. Nature Nanotechnology 2014, 9, 1054–1062.

31. D. Henderson, L. A. Ogilvie, N. Hoyle, U. Keilholz, B. Lange, H. Lehrach, and OncoTrack Consortium. Personalized medicine approaches for colon cancer driven by genomics and systems biology : OncoTrack. Biotechnology Journal 2014, 9(9), 1104-1114.

32. B. Rogez, H. Yang, E. Le Moal, S. Lévêque-Fort, E. Boer-Duchemin, F. Yao, Y.-H. Lee, Y. Zhang, K. D. Wegner, N. Hildebrandt, A. Mayne, and G. Dujardin. Fluorescence Lifetime and Blinking of Individual Semiconductor Nanocrystals on Graphene. Journal of Physical Chemistry C 2014, 118 (32), 18445–18452.

33. K. D. Wegner, F. Morgner, E. Oh, R. Goswami, K. Susumu, M. H. Stewart, I. L. Medintz, and N. Hildebrandt. Three-dimensional solution-phase Förster resonance energy transfer analysis of nanomolar quantum dot bioconjugates with subnanometer resolution. Chemistry of Materials 2014, 26 (14), 4299–4312.

34. P. J. Cywiński, T. Hammann, D. Hühn, W. J. Parak, N. Hildebrandt, and H.-G. Löhmannsröben. Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing. Journal of Biomedical Optics 2014, 19(10), 101506.

35. X. Le Guevel, O. Tagit, C.E. Rodríguez, V. Trouillet, M.P. Leal, and N. Hildebrandt. Ligand Effect on Size, Valence State and Red/Near Infrared Photoluminescence of Bidentate Thiol Gold Nanoclusters. Nanoscale 2014, 6 (14), 8091 - 8099.

36. J. C. Claussen, N. Hildebrandt, K. Susumu, M. Ancona, and I. L. Medintz. Complex Logic Functions Implemented with Quantum-Dot Bionanophotonic Circuits. ACS Applied Materials & Interfaces 2014, 6, 3771-3778.

37. K. D. Wegner, S. Lindén, Z. Jin, T. L. Jennings, R. el Khoulati, P. M. P. van Bergen en Henegouwen, and N. Hildebrandt. Nanobodies and Nanocrystals : Highly sensitive quantum dot-based homogeneous FRET-immunoassay for serum-based EGFR detection. Small 2014, 10 (4), 734-740.

38. J. C. Claussen, W. R. Algar, N. Hildebrandt, K. Susumu, M. G. Ancona, and I. L. Medintz. Biophotonic Logic Devices Based on Quantum Dots and Temporally-Staggered Förster Energy Transfer Relays. Nanoscale 2013, 5, 12156-12170.

39. K. D. Wegner, Z. Jin, S. Lindén, T. L. Jennings, and N. Hildebrandt. Quantum-Dot-Based Förster Resonance Energy Transfer Immunoassay for Sensitive Clinical Diagnostics of Low-Volume Serum Samples. ACS Nano 2013, 7 (8), 7411–7419.

40. K. Nchimi-Nono, K. D. Wegner, S. Lindén, A. Lecointre, L. Ehret-Sabatier, S. Shakir, N. Hildebrandt, and L. J. Charbonnière. Activated Phosphonated Trifunctional Chelates for Highly Sensitive Lanthanide-based FRET Immunoassays Applied to Total Prostate Specific Antigen Detection. Organic & Biomolecular Chemistry 2013, 11, 6493–6501.

41. K. D. Wegner, P. T. Lanh, T. Jennings, E. Oh, V. Jain, S. M. Fairclough, J. M. Smith, E. Giovanelli, N. Lequeux, T. Pons and N. Hildebrandt. Influence of Luminescence Quantum Yield, Surface Coating and Functionalization of Quantum Dots on the Sensitivity of Time-Resolved FRET Bioassays. ACS Applied Materials & Interfaces 2013, 5, 2881-2892.

42. D. Geißler , S. Stufler, H.-G. Löhmannsröben and N. Hildebrandt. Six-Color Time-Resolved Förster Resonance Energy Transfer for Ultrasensitive Multiplexed Biosensing. Journal of the American Chemical Society 2013, 135, 1102-1109.

43. W. R. Algar, A. Malonoski, K. Susumu, M. H. Stewart, N. Hildebrandt, and I. L. Medintz. Multiplexed Protease Sensing Using One Type of Quantum Dot Vector and a Time-Gated Förster Resonance Energy Transfer Relay. Analytical Chemistry 2012, 84 (22), 10136–10146.

44. W. R. Algar, D. Wegner, A. L. Huston, J. B. Blanco-Canosa, M. H. Stewart, A. Armstrong, P. E. Dawson, N. Hildebrandt and I. L. Medintz. Quantum Dots as Simultaneous Acceptors and Donors in Time-Gated Förster Resonance Energy Transfer Relays : Characterization and Biosensing. Journal of the American Chemical Society 2012, 134, 1876−1891.

45. F. Morgner, S. Stufler, D. Geißler, I. L. Medintz, W. R. Algar, K. Susumu, M. H. Stewart, J. B. Blanco-Canosa, P. E. Dawson and N. Hildebrandt. Terbium to Quantum Dot FRET Bioconjugates for Clinical Diagnostics : Influence of Human Plasma on Optical and Assembly Properties. Sensors 2011, 11(10), 9667-9684.

46. A. Kupstatt, M.U. Kumke, N. Hildebrandt. Miniaturization of sensitive homogeneous time-resolved fluoroimmunoassays (TR-FIA) for point-of-care testing (POCT). Analyst 2011, 136 (5), 1029-1035.

47. P. J. Cywinski, A. J. Moro, T. Ritschel, N. Hildebrandt, H.-G. Löhmannsröben. Sensitive and selective fluorescence detection of guanosine nucleotides by nanoparticles conjugated with a naphthyridine. Analytical and Bioanalytical Chemistry 2011, 399(3), 1215-1222.

48. C. Tan, N. Gajovic-Eichelmann, R. Polzius, N. Hildebrandt, F.F. Bier. Direct detection of Δ9-tetrahydrocannabinol in aqueous samples using a homogeneous increasing fluorescence immunoassay (HiFi). Analytical and Bioanalytical Chemistry 2010, 398, 2133-2140.

49. F. Morgner, D. Geißler, S. Stufler, N.G. Butlin, H.-G. Löhmannsröben, N. Hildebrandt. A Quantum Dot-based Molecular Ruler for Multiplexed Optical Analysis. Angewandte Chemie - International Edition 2010, 49(41), 7570-7574.

50. F. Sellrie, M. Beck, N. Hildebrandt, B. Micheel. A homogeneous time-resolved fluoroimmunoassay (TR-FIA) using antibody mediated luminescence quenching. Analytical Methods 2010, 2, 1298–1301.

51. D. Geißler, L.J. Charbonnière, R.F. Ziessel, N.G. Butlin, H.-G. Löhmannsröben, N. Hildebrandt. Quantum Dot Biosensors for Ultra-Sensitive Multiplexed Diagnostics. Angewandte Chemie - International Edition 2010, 49(8), 1396-1401.

52. P. Kadjane, M. Starck, F. Camerel, D. Hill, N. Hildebrandt, R. Ziessel, L.J. Charbonnière. Divergent Approach to a Large Variety of Versatile Luminescent Lanthanide Complexes. Inorganic Chemistry 2009, 48, 4601–4603.

53. N. Hildebrandt, L.J. Charbonnière, H.-G. Löhmannsröben. Time-Resolved Analysis of a Highly Sensitive Förster Resonance Energy Transfer (FRET) Immunoassay Using Terbium Complexes as Donors and Quantum Dots as Acceptors. Journal of Biomedicine and Biotechnology 2007, Article ID 79169.

54. L.J. Charbonnière, N. Hildebrandt, R.F. Ziessel, H.-G. Löhmannsröben. Lanthanides to Quantum Dots Resonance Energy Transfer in Time-Resolved FluoroImmunoAssays and Luminescence Microscopy. Journal of the American Chemical Society 2006, 128(39), 12800-12809.

55. N. Hildebrandt, L.J. Charbonnière, M. Beck, R.F. Ziessel, H.-G. Löhmannsröben. Quantum Dots As Efficient Energy Acceptors in a Time-Resolved Fluoroimmunoassay. Angewandte Chemie - International Edition 2005, 44(46), 7612-7615.

Conference proceedings

56. J. C. Claussen, W. R. Algar, N. Hildebrandt, K. Susumu, M. G. Ancona, and I. L. Medintz. Enhancing molecular logic through modulation of temporal and spatial constraints with quantum dot-based systems that use fluorescent (Förster) resonance energy transfer. Proceedings of SPIE 8817, 2013, DOI:10.1117/12.2024287.

57. D. Hill, C. Ast, H.-G. Löhmannsröben, A. Zulqurnain, W. J. Parak, N. Hildebrandt. Size Determination of Quantum Dots with Fluorescence Correlation Spectroscopy. Proceedings of SPIE 7909, 2011, DOI : 10.1117/12.874660.

58. D. Wegner, D. Geißler, S. Stufler, H.-G. Löhmannsröben, N. Hildebrandt. Time-resolved and steady-state FRET spectroscopy on commercial biocompatible quantum dots. Proceedings of SPIE 7909, 2011, DOI : 10.1117/12.874760.

59. M. Kollosche, G. Kofod, S. Doering, N. Hildebrandt, J. Stumpe. Optical transmission gratings tuned by electro active polymers. Proceedings of the 2010 IEEE International Conference on Solid Dielectrics.

60. S. Doering, M. Kollosche, N. Hildebrandt, J. Stumpe, G. Kofod. Tunable diffractive optical elements on various electro active polymers. Proceedings of SPIE 7716, 2010, DOI : 10.1117/12.854258.

61. S. Doering, T. Rabe, R. Rosenhauer, O. Kulikovska, N. Hildebrandt, J. Stumpe. Azobenzene based surface relief gratings for thin film distributed feedback lasers. Proceedings of SPIE 7722, 2010, DOI : 10.1117/12.854693.

62. D. Geißler, D. Hill, H.-G. Löhmannsröben, E. Thomas, A. Lavigne, B. Darbouret, E. Bois, L.J. Charbonnière, R.F. Ziessel, N. Hildebrandt. Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays. Proceedings of SPIE 7572, 2010, DOI : 10.1117/12.842731.

63. D. Geißler, H.-G. Löhmannsröben, L.J. Charbonnière, R.F. Ziessel, N.G. Butlin, I.L. Medintz, H. Mattoussi, N. Hildebrandt. Optical size determination of quantum dots using FRET with terbium complexes as donors. Proceedings of SPIE 7575, 2010, DOI : 10.1117/12.842750.

64. N. Hildebrandt. Energy transfer from terbium complexes to quantum dots – The advantage of independent donor and acceptor decay time analysis for investigations on FRET distance dependence. Proceedings of SPIE 7575, 2010, DOI : 10.1117/12.842762.

65. D. Geißler, N.G. Butlin, Hill D, H.-G. Löhmannsröben, N. Hildebrandt. Multiplexed diagnostics and spectroscopic ruler applications with terbium to quantum dots FRET. Proceedings of SPIE 7368, 2009, DOI : 10.1117/12.831631.

66. D. Geißler, N. Hildebrandt, L.J. Charbonnière, R.F. Ziessel, H.-G. Löhmannsröben. Quantum dots as FRET acceptors for highly sensitive multiplexing immunoassays. Proceedings of SPIE 7189, 2009, DOI : 10.1117/12.809444.

67. N. Hildebrandt, L.J. Charbonnière, R.F. Ziessel, H.-G. Löhmannsröben. Homogeneous FRET Immunoassay Based on Lanthanides to Quantum Dots Energy Transfer. Proceedings of SPIE 6448, 2007, DOI : 10.1117/12.700206.

68. M. Niederkrüger, C. Salb, G. Marowsky, M. Beck, N. Hildebrandt, H.-G. Löhmannsröben. Improvement of a Fluorescence Immunoassay with a Compact Diode-Pumped Solid State Laser at 315 nm. Proceedings of SPIE 6380, 2006, DOI : 10.1117/12.685993.

69. N. Hildebrandt, L.J. Charbonnière, R.F. Ziessel, H.-G. Löhmannsröben. Quantum Dots as Resonance Energy Transfer Acceptors for Monitoring Biological Interactions. Proceedings of SPIE 6191, 2006, DOI : 10.1117/12.660660.

70. M. Beck, N. Hildebrandt, H.-G. Löhmannsröben. Quantum Dots as Acceptors in FRET Assays Containing Serum. Proceedings of SPIE 6191, 2006, DOI : 10.1117/12.662722.

71. H.-G. Löhmannsröben, M. Beck, N. Hildebrandt, E. Schmälzlin, J.T. van Dongen. Laser-Based Fluoroimmuno Analysis and In-Vivo Optical Oxygen Monitoring. Proceedings of SPIE 6157, 2006, DOI : 10.1117/12.663583.

72. N. Hildebrandt, R. Flehr, E. Bois, H.-G. Löhmannsröben. Optimized Homogeneous Immunoassay Based on XeCI-Laser Excited Förster Resonance Energy Transfer. IEEE CLEO Europe 2005, DOI : 10.1109/CLEOE.2005.1568413.

Book chapters

1. J. Zwier, N. Hildebrandt. Time-gated FRET detection for multiplexed biosensing. In : Chris D. Geddes, editor : Reviews in Fluorescence 2016. Springer International 2017, ISBN 978-3-319-48259-0.

2. N. Hildebrandt. How to apply FRET - From experimental design to data analysis. In : I. Medintz and N. Hildebrandt, editors : FRET – Förster Resonance Energy Transfer. From Theory to Applications”, Wiley-VCH, Germany 2014, ISBN 978-3-527-32816-1.

3. J. C. Claussen, N. Hildebrandt, I. L. Medintz. FRET-based Cellular Sensing with Genetically Encoded Fluorescent Indicators. In : I. Medintz and N. Hildebrandt, editors : FRET – Förster Resonance Energy Transfer. From Theory to Applications”, Wiley-VCH, Germany 2014, ISBN 978-3-527-32816-1.

4. D. Geißler, N. Hildebrandt. Semiconductor Quantum Dots as FRET Acceptors for Multiplexed Diagnostics and Molecular Ruler Application. In : Nano-Biotechnology for Biomedical and Diagnostic Research, Advances in Experimental Medicine and Biology, Vol. 733 (Editors : E. Zahavy, A. Ordentlich, S. Yitzhaki, A. Shafferman). Springer Verlag, Germany, 2012. pp. 75-86. ISBN 978-94-007-2554-6

5. Z. Jin, N. Hildebrandt. Quantum Dot Nanoparticles for In Vitro Sensing, In : Jesus M. de la Fuente, Joseph, Poulouse , editors : Nanobiotechnology, 4, FNS, UK : Elsevier, 2012, pp. 291-307. ISBN 978-0-12-415769-9


1. N. Hildebrandt, D. Geiβler, H.-G. Löhmannsröben, L.J. Charbonnière, R.F. Ziessel, E. Bois. Method for detecting an analyte in a sample by multiplexing FRET analysis and Kit, WO/2010/084015, 07/2010.

2. W. R. Algar, A. L. Huston, N. Hildebrandt and I. L. Medintz. Spectro-Temporal Optical Encoding of Information Using a Time-Gated Fluorescence Resonance Energy Transfer (FRET). US 8,476,083 B1, 07/2013.

3. Z. Jin and N. Hildebrandt. Multiplexed homogeneous oligonucleotide detection. EP 2,949,759 A1, 12/2015.

4. X. Qiu, N. Hildebrandt. Kit and method for detecting or quantifying one or multiple nucleic acid targets. EP16305582. 05/2016.

5. N. Hildebrandt, L.J. Charbonnière, P. Reiss, S. Bhuckory, L. Mattera, K.D. Wegner. Nouveaux conjugués comprenant des nanocristaux semi-conducteurs et leur méthode de préparation. FR 1660647. 11/2016.

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