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Accueil > Départements > Biologie Cellulaire > Sébastien THOMINE : Approches Intégratives du Transport des Ions

Publications de l’équipe

Publications des membres de l’équipe depuis 2008 :


  • A. Agorio, J. Giraudat, M. W. Bianchi, J. Marion, C. Espagne, L. Castaings, F. Lelièvre, C. Curie, S. Thomine, et S. Merlot, « Phosphatidylinositol 3-phosphate–binding protein AtPH1 controls the localization of the metal transporter NRAMP1 in Arabidopsis », Proceedings of the National Academy of Sciences, p. 201702975, avr. 2017.
    Mots-clés : BIOCELL, DYNBSJ, late endosome, metal transport, MINION, NRAMP, phosphatidylinositol 3-phosphate, vacuole.

  • C. Eisenach, U. Baetz, N. V. Huck, J. Zhang, A. De Angeli, G. Beckers, et E. Martinoia, « ABA-Induced Stomatal Closure Involves ALMT4, a Phosphorylation-Dependent Vacuolar Anion Channel of Arabidopsis », The Plant Cell, 2017.
    Résumé : Stomatal pores are formed between a pair of guard cells, and allow plant uptake of CO2 and water evaporation. Their aperture depends on changes in osmolyte concentration of guard cell vacuoles, specifically of K+ and Mal2-. Efflux of Mal2- from the vacuole is required for stomatal closure; however, it is not clear how the anion is released. Here we report the identification of ALMT4 (ALUMINIUM ACTIVATED MALATE TRANSPORTER 4) as an Arabidopsis thaliana ion channel that can mediate Mal2- release from the vacuole and is required for stomatal closure in response to abscisic acid (ABA). Knock-out mutants showed impaired stomatal closure in response to the drought-stress hormone ABA and increased whole-plant wilting in response to drought and ABA. Electrophysiological data show that ALMT4 can mediate Mal2- efflux and that the channel activity is dependent on a phosphorylatable C-terminal serine. Dephosphomimetic mutants of ALMT4 S382 showed increased channel activity and Mal2- efflux. Reconstituting the active channel in almt4 mutants impaired growth and stomatal opening. Phosphomimetic mutants were electrically inactive and phenocopied the almt4 mutants. Surprisingly, S382 can be phosphorylated by mitogen-activated protein (MAP) kinases in vitro. In brief, ALMT4 likely mediates Mal2- efflux during ABA-induced stomatal closure and its activity depends on phosphorylation.
    Mots-clés : BIOCELL, MINION.

  • C. Eisenach et A. De Angeli, « Ion Transport at the Vacuole During Stomatal Movements », Plant Physiology, p. pp.00130.2017, avr. 2017.



  • A. Aznar, N. W. G. Chen, S. Thomine, et A. Dellagi, « Immunity to plant pathogens and iron homeostasis », Plant Science, vol. 240, p. 90-97, 2015.
    Mots-clés : BIOCELL, Homeostasis, Iron, MINION, Pathogen, Phenolics, plant defense, Plant Diseases, Plant Immunity, Reactive Oxygen Species, Siderophores.

  • C. Berrier, R. Peyronnet, J. - M. Betton, G. Ephritikhine, H. Barbier-Brygoo, J. - M. Frachisse, et A. Ghazi, « Channel characteristics of VDAC-3 from Arabidopsis thaliana », Biochemical and Biophysical Research Communications, vol. 459, nᵒ 1, p. 24-28, 2015.
    Mots-clés : Arabidopsis, Arabidopsis Proteins, BIOCELL, Cell-free synthesis, Cell-Free System, Channel, Electrophysiological Phenomena, Escherichia coli, Lipid Bilayers, MINION, Protein Engineering, Recombinant Proteins, VDAC, Voltage-Dependent Anion Channels.

  • E. M. Bruch, S. Thomine, L. C. Tabares, et S. Un, « Variations in Mn(II) speciation among organisms: what makes D. radiodurans different. », Metallomics, vol. 7, nᵒ 1, p. 136-144, 2015.
    Mots-clés : B3S, BHFMR, BIOCELL, Deinococcus, Escherichia coli, Manganese, MINION, Nuclear Magnetic Resonance, Biomolecular, Nucleic Acids, Phosphates, Saccharomyces cerevisiae, Water.

  • E. M. Bruch, M. T. Warner, S. Thomine, L. C. Tabares, et S. Un, « Pulse Electron Double Resonance Detected Multinuclear NMR Spectra of Distant and Low Sensitivity Nuclei and Its Application to the Structure of Mn(II) Centers in Organisms », The Journal of Physical Chemistry B, vol. 119, nᵒ 43, p. 13515-13523, oct. 2015.
    Mots-clés : B3S, BHFMR, BIOCELL, Deinococcus, Electron Spin Resonance Spectroscopy, Escherichia coli, Manganese, MINION, Models, Molecular, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Organometallic Compounds, Quantum Theory.

  • C. M. Grison, S. Jackson, S. Merlot, A. Dobson, et C. Grison, « Rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the Anthyllis vulneraria Zn-hyperaccumulator », INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 65, nᵒ Pt 5, p. 1525-1530, mai 2015.
    Mots-clés : Bacterial Typing Techniques, Base Composition, BIOCELL, DNA, Bacterial, Fabaceae, Fatty Acids, France, Mining, MINION, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids, Phylogeny, Rhizobium, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Symbiosis, Zinc.

  • A. Grondin, O. Rodrigues, L. Verdoucq, S. Merlot, N. Leonhardt, et C. Maurel, « Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation », The Plant Cell, vol. 27, nᵒ 7, p. 1945-1954, 2015.
    Mots-clés : Abscisic Acid, Animals, Aquaporins, Arabidopsis Proteins, BIOCELL, Cell Membrane Permeability, Enzyme Activation, Genetic Complementation Test, MINION, Movement, Mutation, Oocytes, Phosphorylation, Phosphoserine, Plant Stomata, Protein Kinases, Protoplasts, Reactive Oxygen Species, Xenopus.

  • V. Mary, M. Schnell Ramos, C. Gillet, A. L. Socha, J. Giraudat, A. Agorio, S. Merlot, C. Clairet, S. A. Kim, T. Punshon, M. L. Guerinot, et S. Thomine, « Bypassing Iron Storage in Endodermal Vacuoles Rescues the Iron Mobilization Defect in the <i>natural resistance associated-macrophage protein3natural resistance associated-macrophage protein4</i> Double Mutant », Plant Physiology, vol. 169, nᵒ 1, p. 748-759, 2015.
    Mots-clés : Alleles, Arabidopsis, Arabidopsis Proteins, BIOCELL, Biological Transport, Cation Transport Proteins, Cotyledon, Gene Expression Regulation, Plant, Gene Knockout Techniques, Genes, Plant, Genes, Suppressor, Germination, Iron, MINION, Models, Biological, Mutagenesis, Mutation, Phenotype, Plant Epidermis, RNA, Messenger, Seedlings, Spectrometry, X-Ray Emission, Subcellular Fractions, Vacuoles.

  • M. Migocka, A. Kosieradzka, A. Papierniak, E. Maciaszczyk-Dziubinska, E. Posyniak, A. Garbiec, et S. Filleur, « Two metal-tolerance proteins, MTP1 and MTP4, are involved in Zn homeostasis and Cd sequestration in cucumber cells », Journal of Experimental Botany, vol. 66, nᵒ 3, p. 1001-1015, févr. 2015.

  • M. Pottier, V. S. García de la Torre, C. Victor, L. C. David, M. Chalot, et S. Thomine, « Genotypic variations in the dynamics of metal concentrations in poplar leaves: A field study with a perspective on phytoremediation », Environmental Pollution, vol. 199, p. 73-82, 2015.
    Mots-clés : BIOCELL, Biodegradation, Environmental, Cadmium, Copper, Environmental Pollution, HMA, metals, MINION, NRAMP, Plant Leaves, Populus, Soil, Soil Pollutants, Trace Elements, Zinc.

  • M. Pottier, R. Oomen, C. Picco, J. Giraudat, J. Scholz-Starke, P. Richaud, A. Carpaneto, et S. Thomine, « Identification of mutations allowing Natural Resistance Associated Macrophage Proteins (NRAMP) to discriminate against cadmium », The Plant Journal, vol. 83, nᵒ 4, p. 625-637, 2015.
    Mots-clés : Arabidopsis, Arabidopsis Proteins, Arabidopsis thaliana, BIOCELL, Cadmium, Gene Expression Regulation, Plant, metals, MINION, Mutation, Plant Proteins, transition metals, transport, vacuole, Vacuoles.
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-  Pottier, M., Masclaux-Daubresse, C., Yoshimoto, K., & Thomine, S. (2014). Autophagy as a possible mechanism for micronutrient remobilization from leaves to seeds. Front Plant Sci, 5, 11. doi:10.3389/fpls.2014.00011

- Le Bars, R., Marion, J., Le Borgne, R., Satiat-Jeunemaitre, B., & Bianchi, M. W. (2014). ATG5 defines a phagophore domain connected to the endoplasmic reticulum during autophagosome formation in plants. Nat Commun, 5, 4121. doi:10.1038/ncomms5121

- Le Bars, R., Marion, J., Satiat-Jeunemaitre, B., & Bianchi, M. W. (2014). Folding into an autophagosome : ATG5 sheds light on how plants do it. Autophagy, 10(10), 1861-1863. doi:10.4161/auto.29962

-  Aznar, A., Chen, N. W., Rigault, M., Riache, N., Joseph, D., Desmaele, D., Mouille, G., Boutet, S., Soubigou-Taconnat, L., Renou, J. P., Thomine, S., Expert, D., & Dellagi, A. (2014). Scavenging iron : a novel mechanism of plant immunity activation by microbial siderophores. Plant Physiol, 164(4), 2167-2183. doi:10.1104/pp.113.233585

-  Zhang, J., Martinoia, E., & De Angeli, A. (2014). Cytosolic nucleotides block and regulate the Arabidopsis vacuolar anion channel AtALMT9. J Biol Chem, 289(37), 25581-25589. doi:10.1074/jbc.M114.576108

-  Michaud, M., Ubrig, E., Filleur, S., Erhardt, M., Ephritikhine, G., Marechal-Drouard, L., & Duchene, A. M. (2014). Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology. Proc Natl Acad Sci U S A, 111(24), 8991-8996. doi:10.1073/pnas.1402588111

-  Migocka, M., Papierniak, A., Maciaszczyk-Dziubinska, E., Pozdzik, P., Posyniak, E., Garbiec, A., & Filleur, S. (2014). Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana. J Exp Bot, 65(18), 5367-5384. doi:10.1093/jxb/eru295

-  Lanquar, V., Grossmann, G., Vinkenborg, J. L., Merkx, M., Thomine, S., & Frommer, W. B. (2014). Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology. New Phytol, 202(1), 198-208. doi:10.1111/nph.12652

-  Durand-Smet, P., Chastrette, N., Guiroy, A., Richert, A., Berne-Dedieu, A., Szecsi, J., Boudaoud, A., Frachisse, J. M., Bendahmane, M., Hamant, O., & Asnacios, A. (2014). A comparative mechanical analysis of plant and animal cells reveals convergence across kingdoms. Biophys J, 107(10), 2237-2244. doi:10.1016/j.bpj.2014.10.023

-  Peyronnet, R., D. Tran, T. Girault & J.M. Frachisse (2014). Mechanosensitive channels : feeling tension in a world under pressure. Front. Plant Sci. doi : 10.3389/fpls.2014.00558.

-  Wege, S., A. De Angeli, M.J. Droillard, L. Kroniewicz, S. Merlot, D. Cornu, F. Gambale, E. Martinoia, H. Barbier-Brygoo, S. Thomine, N. Leonhardt, S. Filleur (2014). Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid. Sci Signal. 7(333):ra65. doi : 10.1126/scisignal.2005140.

-  Merlot, S., L. Hannibal, S. Martins, L. Martinelli, H. Amir, M. Lebrun, S. Thomine (2014). The metal transporter PgIREG1 from the hyperaccumulator Psychotria gabriellae is a candidate gene for nickel tolerance and accumulation. J. Exp. Bot. 65(6):1551-1564. doi : 10.1093/jxb/eru025.


-  Jaffré, T., Y. Pillon, S. Thomine, S. Merlot (2013).The metal hyperaccumulators from New Caledonia can broaden our understanding of nickel accumulation in plants. Front Plant Sci. 4:279. doi : 10.3389/fpls.2013.00279.

-  Thomine, S., G. Vert (2013). Iron transport in plants : better be safe than sorry. Current Opinion in Plant Biology 16(3):322-7. doi : 10.1016/j.pbi.2013.01.003.

-  De Angeli, A., Baetz, U., Francisco, R., Zhang, J., Chaves, M. M., & Regalado, A. (2013). The vacuolar channel VvALMT9 mediates malate and tartrate accumulation in berries of Vitis vinifera. Planta, 238(2), 283-291. doi:10.1007/s00425-013-1888-y

-  De Angeli, A., Zhang, J., Meyer, S., & Martinoia, E. (2013). AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis. Nat Commun, 4, 1804. doi:10.1038/ncomms2815

-  Zhang, J., Baetz, U., Krugel, U., Martinoia, E., & De Angeli, A. (2013). Identification of a probable pore-forming domain in the multimeric vacuolar anion channel AtALMT9. Plant Physiol, 163(2), 830-843. doi:10.1104/pp.113.219832


-  Clemens, S., M.M. Aarts, S. Thomine, N. Verbruggen (2012). Plant science : the key to preventing slow cadmium poisoning. Trends in Plant Science 18(2):92-9.

-  Gan, Y., Bernreiter, A., Filleur, S., Abram, B., & Forde, B. G. (2012). Overexpressing the ANR1 MADS-box gene in transgenic plants provides new insights into its role in the nitrate regulation of root development.
Plant Cell Physiol, 53(6), 1003-1016. doi:10.1093/pcp/pcs050

-  Robert, N., d’Erfurth, I., Marmagne, A., Erhardt, M., Allot, M., Boivin, K., Gissot, L., Monachello, D., Michaud, M., Duchene, A. M., Barbier-Brygoo, H., Marechal-Drouard, L., Ephritikhine, G., & Filleur, S. (2012). Voltage-dependent-anion-channels (VDACs) in Arabidopsis have a dual localization in the cell but show a distinct role in mitochondria. Plant Mol Biol, 78(4-5), 431-446. doi:10.1007/s11103-012-9874-5


-  Kollist, H., M. Jossier, K. Laanemets, S. Thomine (2011). Anion channels in plant cells. FEBS J. 278(22):4277-4292.

-  Bolte, S., V. Lanquar, M-N. Soler, A. Beebo, B. Satiat-Jeunemaître, K. Bouhidel, S. Thomine (2011). Distinct Lytic Vacuolar Compartments are Embedded Inside the Protein Storage Vacuole of Dry and Germinating Arabidopsis thaliana Seeds. Plant Cell Physiol. 52(7):1142-52.

-  Barbier-Brygoo, H., A. De Angeli, S. Filleur, J-M. Frachisse, F. Gambale, S. Thomine, S. Wege (2011). Anion channels/transporters in plants : from molecular bases to regulatory networks]. Annu Rev Plant Biol. 62:25-51.


-  Diatloff, E., R. Peyronnet, J. Colcombet, S. Thomine, H. Barbier-Brygoo, J-M. Frachisse (2010). R type anion channel : a multifunctional channel seeking its molecular identity]. Plant Signal. Behav. 5:22-27.

-  Thomine, S., H. Barbier-Brygoo (2010). Structural biology : A peep through anion channels] (Comment) Nature 467(7319):1058-9.

-  Wege, S., M. Jossier, S. Filleur, S. Thomine, H. Barbier-Brygoo, F. Gambale, A. De Angeli (2010). The proline 160 in the selectivity filter of the Arabidopsis NO3-/H+ exchanger AtCLCa is essential for nitrate accumulation in planta. Plant J. 63(5):861-9.

-  Migeon, A., D. Blaudez, O. Wilkins, B. Montanini, M.M. Campbell, P. Richaud, S. Thomine, M. Chalot M. (2010). Genome-wide analysis of plant metal transporters, with an emphasis on poplar. Cell. Mol. Life Sci.67(22):3763-84.


-  De Angeli, A., O. Moran, S. Wege, S. Filleur, G. Ephritikhine, S. Thomine, H. Barbier-Brygoo & F. Gambale (2009). ATP binding to the C terminus of the Arabidopsi thaliana nitrate/proton antiporter, AtCLCa, regulates nitrate transport into plant vacuoles. J Biol Chem. 284(39):26526-32.

-  De Angeli, A., D. Monachello, G. Ephritikhine, J-M. Frachisse., S. Thomine., F. Gambale & H. Barbier-Brygoo(2009). Review. . Philos Trans R Soc Lond B Biol Sci 364(1514):195-201.


-  Peyronnet, R., E.S. Haswell, H. Barbier-Brygoo, J-M. Frachisse (2008). AtMSL9 and AtMSL10 : Sensors of plasma membrane tension in Arabidopsis roots. Plant Signaling & Behavior3(9):726-9.

-  Haswell, E.S., R. Peyronnet, H. Barbier-Brygoo, E.M. Meyerowitz & J-M. Frachisse (2008). Two MscS homologs provide mechanosensitive channel activities in the Arabidopsis root. Curr. Biol. 18(10):730-4.

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