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Accueil > Départements > Microbiologie > Pierre TISSIERES : Endotoxines, Structures et Réponses de l’hôte

Publications de l’équipe


  • A. Boet, G. Jourdain, S. Demontoux, S. Hascoet, P. Tissieres, C. Rucker-Martin, et D. De Luca, « Basic Hemodynamic Monitoring Using Ultrasound or Electrical Cardiometry During Transportation of Neonates and Infants », Pediatric Critical Care Medicine: A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, 2017.
    Résumé : OBJECTIVES: Electrical cardiometry and heart ultrasound might allow hemodynamic evaluation during transportation of critically ill patients. Our aims were 1) to test feasibility of stroke volume monitoring using electrical cardiometry or ultrasound during transportation and 2) to investigate if transportation impacts on electrical cardiometry and ultrasound reliability. DESIGN: Prospective, pragmatic, feasibility cohort study. SETTING: Mobile ICUs specialized for neonatal and pediatric transportation. PATIENTS: Thirty hemodynamically stable neonates and infants. INTERVENTIONS: Patients enrolled underwent paired stroke volume measurements (180 before/after and 180 during the transfer) by electrical cardiometry (SVEC) and ultrasound (SVUS). MEASUREMENTS AND MAIN RESULTS: No problems or malfunctioning occurred neither with electrical cardiometry nor with ultrasound. Ultrasound lasted on average 90 (10) seconds, while 45 (15) seconds were needed to instigate electrical cardiometry monitoring. Coefficient of variation was higher for SVUS (before/after: 0.57; during: 0.66) than for SVEC (before/after: 0.38; during: 0.36). Correlations between SVEC and SVUS before/after and during the transfer were r equal to 0.57 and r equal to 0.8, respectively (p always < 0.001). Bland-Altman analysis showed that stroke volume tends to be higher if measured by electrical cardiometry. SVEC measured before (5.5 [2.4] mL), during (5.4 [2.4] mL), and after the transfer (5.4 [2.3] mL) are similar (p = 0.955); same applies for SVUS before (2.6 [1.5] mL), during (2.4 [2] mL), and after (2.9 [2] mL) the transfer (p = 0.268). CONCLUSIONS: Basic hemodynamic monitoring is feasible during pediatric and neonatal transportation both with electrical cardiometry and ultrasound. These two techniques show comparable reliability, although stroke volume was higher if measured by electrical cardiometry. The transportation itself does not affect the reliability of stroke volume measurements.
    Mots-clés : ESHR, MICROBIO.

  • A. Breton, A. Novikov, R. Martin, P. Tissieres, et M. Caroff, « Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies », Journal of Lipid Research, vol. 58, nᵒ 3, p. 543-552, 2017.
    Résumé : Vitreoscilla filiformis is a Gram-negative bacterium isolated from spa waters and described for its beneficial effects on the skin. We characterized the detailed structure of its lipopolysaccharide (LPS) lipid A moiety, an active component of the bacterium that contributes to the observed skin activation properties. Two different batches differing in postculture cell recovery were tested. Chemical analyses and mass spectra, obtained before and after mild-alkali treatments, revealed that these lipids A share the common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxydecanoic acid in an amide linkage. Short-chain FAs, hydroxydecanoic and dodecanoic acid, were found in a 2:1 ratio. The two lipid A structures differed by the relative amount of the hexa-acyl molecular species and phosphoethanolamine substitution of the phosphate groups. The two V. filiformis LPS batches induced variable interleukin-6 and TNF-α secretion by stimulated myelomonocytic THP-1 cells, without any difference in reactive oxygen species production or activation of caspase 3/7. Other different well-known highly purified LPS samples were characterized structurally and used as standards. The structural data obtained in this work explain the low inflammatory response observed for V. filiformis LPS and the previously demonstrated beneficial effects on the skin.
    Mots-clés : cytokines, ESHR, lipid biochemistry, lipopolysaccharide, Mass Spectrometry, MICROBIO, skin, Toll-like receptor, V. filiformis.

  • D. De Luca, A. H. van Kaam, D. G. Tingay, S. E. Courtney, O. Danhaive, V. P. Carnielli, L. J. Zimmermann, M. C. J. Kneyber, P. Tissieres, J. Brierley, G. Conti, J. J. Pillow, et P. C. Rimensberger, « The Montreux definition of neonatal ARDS: biological and clinical background behind the description of a new entity », The Lancet. Respiratory Medicine, 2017.
    Résumé : Acute respiratory distress syndrome (ARDS) is undefined in neonates, despite the long-standing existing formal recognition of ARDS syndrome in later life. We describe the Neonatal ARDS Project: an international, collaborative, multicentre, and multidisciplinary project which aimed to produce an ARDS consensus definition for neonates that is applicable from the perinatal period. The definition was created through discussions between five expert members of the European Society for Paediatric and Neonatal Intensive Care; four experts of the European Society for Paediatric Research; two independent experts from the USA and two from Australia. This Position Paper provides the first consensus definition for neonatal ARDS (called the Montreux definition). We also provide expert consensus that mechanisms causing ARDS in adults and older children-namely complex surfactant dysfunction, lung tissue inflammation, loss of lung volume, increased shunt, and diffuse alveolar damage-are also present in several critical neonatal respiratory disorders.
    Mots-clés : ESHR, MICROBIO.

  • U. Lausten-Thomsen, Z. Merchaoui, C. Dubois, S. Eleni Dit Trolli, N. Le Saché, M. Mokhtari, et P. Tissières, « Ultrasound-Guided Subclavian Vein Cannulation in Low Birth Weight Neonates », Pediatric Critical Care Medicine: A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, vol. 18, nᵒ 2, p. 172-175, 2017.
    Résumé : OBJECTIVES: Central venous access in critically ill, small infants remains technically challenging even in experienced hands. Several vascular accesses exist, but the subclavian vein is often preferred for central venous catheter insertion in infants where abdominal malformation and/or closure of the vein preclude the use of umbilical venous catheters, as catheterization of the subclavian vein is easier in very short necks than the internal jugular vein for age-related anatomical reasons. The subclavian vein approach is yet relatively undescribed in low birth weight infants (i.e., < 2,500 g), and this study aims to explore the feasibility of this technique in very small infants. DESIGN: Retrospective data collection of prospectively registered data on central venous catheter insertion in infants. SETTING: Neonatal ICU and PICU at a university hospital. PATIENTS: One hundred and five newborn children hospitalized in at the ICU. INTERVENTIONS: An ultrasound-guided supraclavicular approach was applied on all infants who had an subclavian vein catheterization during a 30-month period from January 2013 to July 2015. MEASUREMENTS AND MAIN RESULTS: One hundred seven supraclavicular subclavian vein catheters were placed in 105 children weighing less than 5,000 g. Among those, 40 patients weighed less than 2,500 g and 10 patients weighed less than 1,500 g. Successful central venous catheter insertion, defined as the correct placement of a functional double-lumen catheter (3F or 4F), was obtained in 97.3%. All three registered failed attempts were due to hematomas from venous bleeding and occurred in infants weighing greater than 2,500 g. No case of accidental arterial puncture or pleural puncture was registered. CONCLUSIONS: This large series of subclavian vein catheterizations in small infants demonstrates the feasibility of subclavian vein catheterizations even in very small neonates weighing less than 1,500 g.
    Mots-clés : ESHR, MICROBIO.

  • U. Lausten-Thomsen et P. Tissieres, « Mothers' Milk-Can We Improve the Neonatal Immunity Response With the Oldest Recipe in the Mammal's Cookbook? », Pediatric Critical Care Medicine: A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, vol. 18, nᵒ 9, p. 898-899, 2017.

  • M. Levy, N. Le Sache, M. Mokhtari, G. Fagherazzi, G. Cuzon, B. Bueno, V. Fouquet, A. Benachi, S. Eleni Dit Trolli, et P. Tissieres, « Sepsis risk factors in infants with congenital diaphragmatic hernia », Annals of Intensive Care, vol. 7, nᵒ 1, 2017.

  • J. Moh-Klaren, G. Bodivit, M. Jugie, P. Chadebech, L. Chevret, M. Mokhtari, X. Chamillard, P. Gallon, P. Tissières, P. Bierling, R. Djoudi, F. Pirenne, et N. Burin-des-Roziers, « Severe hemolysis after plasma transfusion in a neonate with necrotizing enterocolitis, Clostridium perfringens infection, and red blood cell T-polyagglutination », Transfusion, 2017.
    Résumé : BACKGROUND: Red blood cell (RBC) Thomsen-Friedenreich antigen exposure (T activation) in infants with necrotizing enterocolitis (NEC) has occasionally been associated with posttransfusional intravascular hemolysis thought to be due to anti-T antibodies in the donor plasma. STUDY DESIGN AND METHODS: We describe an infant with NEC and Clostridium perfringens infection complicated by severe hemolysis after plasma transfusion. After this case, infants with confirmed NEC were prospectively evaluated for T activation. We checked for hemolysis in patients with T activation receiving plasma-containing blood products. RESULTS: The infant had received 80 mL of fresh-frozen plasma (FFP). His RBCs displayed strong T activation, and agglutination was observed with four of six ABO-compatible FFP units. A direct antiglobulin test was negative. IgM-class anti-T antibodies were present in small amounts (titer of 8) in the transfused FFP. Anti-T antibodies from the blood donor were not hemolytic in vitro. In the prospective study, T activation was observed in three of 28 infants with NEC (11%). One infant presented moderate T activation and two infants presented very strong T activation but only moderate decreases in sialic acid expression on the RBC membrane. These three infants presented no signs of hemolysis after transfusion with unwashed blood products or FFP. CONCLUSION: Anti-T antibodies are unlikely to be the etiologic factor for the hemolytic reactions observed in infants with NEC and T activation. Massive RBC desialylation and the direct action of bacterial toxins are more probable causes. Strict avoidance of plasma-containing blood products does not seem justified in these infants.
    Mots-clés : ESHR, MICROBIO.

  • A. Novikov, A. Breton, et M. Caroff, « Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides », Methods in Molecular Biology (Clifton, N.J.), vol. 1600, p. 167-186, 2017.
    Résumé : Lipopolysaccharides (LPS) are major components of the external membrane of most Gram-negative bacteria, providing them with an effective permeability barrier. They are essentially composed of a hydrophilic polysaccharide region (PS) linked to a hydrophobic one, termed lipid A. The LPS polysaccharide moiety is divided into the core oligosaccharide (OS) and O-chain repetitive elements. Depending on their individual variable fine structures, LPS may be potent immunomodulators. The lipid A structure is a key determinant for LPS activity. However, the presence of the core region, or at least of the highly charged 3-deoxy-d-manno-oct-2-ulosonic acid molecules, is also important for preserving the native lipid A conformation within individual LPS molecules. We describe herein four rapid and practical micromethods for LPS, lipid A, and core OS structural analyses. The first method allows the direct isolation of lipid A from whole bacteria cell mass; the second describes conditions for the sequential release of fatty acids enabling the characterization of their substitution position in the lipid A backbone, to be determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The third one is a microscale procedure for the mass spectra screening of LPS, lipid A, and PS using triethylamine and citric acid. The fourth method is a chromatography procedure for Rough-type LPS on thin-layer-chromatography. These methods were developed to be coupled to mass-spectrometry (e.g., MALDI-MS) but can also be used with other analytical techniques (e.g., chromatography). Examples are given with reference to two major human pathogens: Bordetella pertussis and Pseudomonas aeruginosa; to one porcine pathogen: Actinobacillus pleuropneumoniae; and to commercial samples of Salmonella Minnesota Re595 LPS.
    Mots-clés : 3-Deoxy-d-manno-oct-2-ulosonic acid, ESHR, Gas chromatography, Lipooligosaccharide, lipopolysaccharide, Matrix-assisted laser desorption/ionization mass spectrometry, MICROBIO, Polyacrylamide gel electrophoresis, Sodium dodecyl sulfate, Thin-layer chromatography, Triethylamine.

  • M. J. Peters, A. Argent, M. Festa, S. Leteurtre, J. Piva, A. Thompson, D. Willson, P. Tissières, M. Tucci, et J. Lacroix, « The intensive care medicine clinical research agenda in paediatrics », Intensive Care Medicine, mars 2017.

  • D. Zhivaki, S. Lemoine, A. Lim, A. Morva, P. - O. Vidalain, L. Schandene, N. Casartelli, M. - A. Rameix-Welti, P. - L. Hervé, E. Dériaud, B. Beitz, M. Ripaux-Lefevre, J. Miatello, B. Lemercier, V. Lorin, D. Descamps, J. Fix, J. - F. Eléouët, S. Riffault, O. Schwartz, F. Porcheray, F. Mascart, H. Mouquet, X. Zhang, P. Tissières, et R. Lo-Man, « Respiratory Syncytial Virus Infects Regulatory B Cells in Human Neonates via Chemokine Receptor CX3CR1 and Promotes Lung Disease Severity », Immunity, vol. 46, nᵒ 2, p. 301-314, 2017.
    Résumé : Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in infants and is characterized by pulmonary infiltration of B cells in fatal cases. We analyzed the B cell compartment in human newborns and identified a population of neonatal regulatory B lymphocytes (nBreg cells) that produced interleukin 10 (IL-10) in response to RSV infection. The polyreactive B cell receptor of nBreg cells interacted with RSV protein F and induced upregulation of chemokine receptor CX3CR1. CX3CR1 interacted with RSV glycoprotein G, leading to nBreg cell infection and IL-10 production that dampened T helper 1 (Th1) cytokine production. In the respiratory tract of neonates with severe RSV-induced acute bronchiolitis, RSV-infected nBreg cell frequencies correlated with increased viral load and decreased blood memory Th1 cell frequencies. Thus, the frequency of nBreg cells is predictive of the severity of acute bronchiolitis disease and nBreg cell activity may constitute an early-life host response that favors microbial pathogenesis.
    Mots-clés : Breg cell, bronchiolitis, ESHR, MICROBIO, Newborn, respiratory syncytial virus.


  • S. M. Basheer, V. Bouchez, A. Novikov, L. A. Augusto, N. Guiso, et M. Caroff, « Structure activity characterization of Bordetella petrii lipid A, from environment to human isolates », Biochimie, vol. 120, p. 87-95, 2016.
    Résumé : Bordetella petrii, a facultative anaerobic species, is the only known member of the Bordetella genus with environmental origin. However it was also recently isolated from humans. The structures of the B. petrii lipid A moieties of the endotoxins were characterized here for the first time for an environmental strain and compared to that of human isolates. Characterization was achieved using chemical analyses, gas chromatography-mass spectrometry, and Matrix Assisted Laser Desorption Ionisation mass spectrometry. The analyses revealed that the different lipid A structures contain a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide as well at the C-3' in ester linkages. Similar to Bordetella pertussis and Bordetella bronchiseptica lipids A, the hydroxytetradecanoic acid at the C-2' position was substituted by tetradecanoic acid. Unlike B. pertussis, the hydroxytetradecanoic acid at the C-2 position was substituted with either 12:0 or 14:0 and/or their 2-OH forms. Depending on the environmental or human origin the structures differed in the length and degree of fatty acid acylation and impacted the IL-6 and TNF-α inflammatory responses tested. In one isolate we showed the presence at the C-3 position of the short-chain 10:0(3-OH), which according to our previous analyses is more characteristic of the human pathogens in the genus like B. pertussis and Bordetella parapertussis.
    Mots-clés : Bordetella, Bordetella petrii, Cell Line, Tumor, Endotoxin, ESHR, Female, Humans, Interleukin-6, Lipid A, Male, Mass Spectrometry, MICROBIO, Monocytes, Structure-Activity Relationship, Structure–activity, Tumor Necrosis Factor-alpha.

  • L. Morin, S. Ray, C. Wilson, S. Remy, M. R. Benissa, N. J. G. Jansen, E. Javouhey, M. J. Peters, M. Kneyber, D. De Luca, S. Nadel, L. J. Schlapbach, G. Maclaren, P. Tissieres, et ESPNIC Refractory Septic Shock Definition Taskforce the Infection Systemic Inflammation Sepsis section of ESPNIC, « Refractory septic shock in children: a European Society of Paediatric and Neonatal Intensive Care definition », Intensive Care Medicine, vol. 42, nᵒ 12, p. 1948-1957, 2016.
    Résumé : PURPOSE: Although overall paediatric septic shock mortality is decreasing, refractory septic shock (RSS) is still associated with high mortality. A definition for RSS is urgently needed to facilitate earlier identification and treatment. We aim to establish a European society of paediatric and neonatal intensive care (ESPNIC) experts' definition of paediatric RSS. METHODS: We conducted a two-round Delphi study followed by an observational multicentre retrospective study. One hundred and fourteen paediatric intensivists answered a clinical case-based, two-round Delphi survey, identifying clinical items consistent with RSS. Multivariate analysis of these items in a development single-centre cohort (70 patients, 30 % mortality) facilitated development of RSS definitions based on either a bedside or computed severity score. Both scores were subsequently tested in a validation cohort (six centres, 424 patients, 11.6 % mortality). RESULTS: From the Delphi process, the draft definition included evidence of myocardial dysfunction and high blood lactate levels despite high vasopressor treatment. When assessed in the development population, each item was independently associated with the need for extracorporeal life support (ECLS) or death. Resultant bedside and computed septic shock scores had high discriminative power against the need for ECLS or death, with areas under the receiver operating characteristics curve of 0.920 (95 % CI 0.89-0.94), and 0.956 (95 % CI 0.93-0.97), respectively. RSS defined by a bedside score equal to or higher than 2 and a computed score equal to or higher than 3.5 was associated with a significant increase in mortality. CONCLUSIONS: This ESPNIC definition of RSS accurately identifies children with the most severe form of septic shock.
    Mots-clés : Acidosis, Cardiac, ESHR, Failure, Lactic, MICROBIO, Paediatrics, Resuscitation, Septic, Shock.

  • W. van Herk, S. el Helou, J. Janota, C. Hagmann, C. Klingenberg, E. Staub, E. Giannoni, P. Tissieres, L. J. Schlapbach, A. M. C. van Rossum, S. B. Pilgrim, et M. Stocker, « Variation in Current Management of Term and Late-preterm Neonates at Risk for Early-onset Sepsis: An International Survey and Review of Guidelines », The Pediatric Infectious Disease Journal, vol. 35, nᵒ 5, p. 494-500, 2016.
    Résumé : BACKGROUND: Uncertainty about the presence of infection results in unnecessary and prolonged empiric antibiotic treatment of newborns at risk for early-onset sepsis (EOS). This study evaluates the impact of this uncertainty on the diversity in management. METHODS: A web-based survey with questions addressing management of infection risk-adjusted scenarios was performed in Europe, North America, and Australia. Published national guidelines (n = 5) were reviewed and compared with the results of the survey. RESULTS: 439 Clinicians (68% were neonatologists) from 16 countries completed the survey. In the low-risk scenario, 29% would start antibiotic therapy and 26% would not, both groups without laboratory investigations; 45% would start if laboratory markers were abnormal. In the high-risk scenario, 99% would start antibiotic therapy. In the low-risk scenario, 89% would discontinue antibiotic therapy before 72 hours. In the high-risk scenario, 35% would discontinue therapy before 72 hours, 56% would continue therapy for 5-7 days, and 9% for more than 7 days. Laboratory investigations were used in 31% of scenarios for the decision to start, and in 72% for the decision to discontinue antibiotic treatment. National guidelines differ considerably regarding the decision to start in low-risk and regarding the decision to continue therapy in higher risk situations. CONCLUSIONS: There is a broad diversity of clinical practice in management of EOS and a lack of agreement between current guidelines. The results of the survey reflect the diversity of national guidelines. Prospective studies regarding management of neonates at risk of EOS with safety endpoints are needed.
    Mots-clés : Australia, Case Management, ESHR, Europe, Guideline Adherence, Guidelines as Topic, Health Policy, Humans, Infant, Newborn, MICROBIO, Neonatal Sepsis, North America, Surveys and Questionnaires.


  • S. Lemoine, B. Jaron, S. Tabka, C. Ettreiki, E. Deriaud, D. Zhivaki, C. Le Ray, O. Launay, L. Majlessi, P. Tissieres, C. Leclerc, et R. Lo-Man, « Dectin-1 activation unlocks IL12A expression and reveals the TH1 potency of neonatal dendritic cells », The Journal of Allergy and Clinical Immunology, vol. 136, nᵒ 5, p. 1355-1368.e1-15, 2015.
    Résumé : BACKGROUND: Early life is characterized by a high susceptibility to infection and a TH2-biased CD4 T-cell response to vaccines. Toll-like receptor (TLR) agonists are currently being implemented as new vaccine adjuvants for TH1 activation, but their translation to the field of pediatric vaccines is facing the impairment of neonatal innate TLR responses. OBJECTIVE: We sought to analyze C-type lectin receptor pathways as an alternative or a coactivator to TLRs for neonatal dendritic cell activation for TH1 polarization. METHODS: Neonatal monocyte-derived dendritic cells (moDCs) were exposed to various combinations of TLR agonists with or without Dectin-1 agonist. IL-12 and IL-23 responses were analyzed at the transcriptional and protein levels after stimulation. The intracellular pathways triggered by combined TLR plus Dectin-1 stimulation was determined by using pharmacologic inhibitors. The capacity of neonatal moDCs to differentiate naive CD4 TH cells was evaluated in cocultures with heterologous neonatal naive T cells. Curdlan was finally tested as an adjuvant within a subunit tuberculosis vaccine in neonatal mice. RESULTS: Simultaneous coactivation through Dectin-1 and TLRs induced robust secretion of IL-12p70 by neonatal moDCs by unlocking transcriptional control on the p35 subunit of IL-12. Both the spleen tyrosine kinase and Raf-1 pathways were involved in this process, allowing differentiation of neonatal naive T cells toward IFN-γ-producing TH1 cells. In vivo a Dectin-1 agonist as adjuvant was sufficient to induce TH1 responses after vaccination of neonatal mice. CONCLUSION: Coactivation of neonatal moDCs through Dectin-1 allows TLR-mediated IL-12p70 secretion and TH1 polarization of neonatal T cells. Dectin-1 agonists represent a promising TH1 adjuvant for pediatric vaccination.
    Mots-clés : adjuvant, Adjuvants, Immunologic, Animals, Animals, Newborn, C-type lectin receptor, Cell Differentiation, Dendritic Cells, ESHR, Humans, Immunity, Innate, innate immunity, Interleukin-12, Interleukin-12 Subunit p35, Lectins, C-Type, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, MICROBIO, Monocytes, Newborn, Proto-Oncogene Proteins c-raf, T(H)1, Th1 Cells, Th2 Cells, Toll-like receptor, Vaccination.

  • F. Longhini, G. Jourdain, F. Ammar, M. Mokthari, C. Boithias, O. Romain, E. Letamendia, P. Tissieres, J. L. Chabernaud, et D. De Luca, « Outcomes of Preterm Neonates Transferred Between Tertiary Perinatal Centers », Pediatric Critical Care Medicine: A Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, vol. 16, nᵒ 8, p. 733-738, 2015.
    Résumé : OBJECTIVE: To verify if preterm neonates transferred between tertiary referral centers have worse outcomes than matched untransferred infants. DESIGN: Cohort study with a historically matched control group. SETTING: Two tertiary-level neonatal ICUs. PATIENTS: Seventy-five neonates per group. INTERVENTIONS: Transfer between tertiary-level neonatal ICUs carried out by a fully equipped transportation team. MEASUREMENTS AND MAIN RESULTS: We measured in-hospital mortality, frequency of intraventricular hemorrhage greater than 2nd grade, periventricular leukomalacia, necrotizing enterocolitis greater than or equal to grade 2, bronchopulmonary dysplasia, composite outcomes (in-hospital mortality/bronchopulmonary dysplasia, in-hospital mortality/intraventricular hemorrhage > 2nd grade, and bronchopulmonary dysplasia/periventricular leukomalacia/intraventricular hemorrhage > 2nd grade), length of neonatal ICU stay, weight at discharge, and time spent on ventilatory support. Seventy-five similar (except for antenatal steroids administration) neonates were enrolled in each cohort. Cohorts did not differ in mortality, bronchopulmonary dysplasia, intraventricular hemorrhage greater than 2nd grade, periventricular leukomalacia, necrotizing enterocolitis greater than or equal to grade 2, any composite outcomes, neonatal ICU stay, weight at discharge, and duration of respiratory support. Results were unchanged adjusting for antenatal steroids. CONCLUSIONS: Neonatal transfer between tertiary-level centers does not impact on clinical outcomes, if performed under optimal conditions.
    Mots-clés : Apgar Score, Birth Weight, Cohort Studies, ESHR, Female, Gestational Age, Hospital Mortality, Humans, Infant, Infant Mortality, Infant, Newborn, Infant, Premature, Intensive Care Units, Neonatal, Length of Stay, Male, MICROBIO, Patient Transfer, Respiration, Artificial, Tertiary Care Centers.

  • W. Zhang-Sun, L. A. Augusto, L. Zhao, et M. Caroff, « Desulfovibrio desulfuricans isolates from the gut of a single individual: structural and biological lipid A characterization », FEBS letters, vol. 589, nᵒ 1, p. 165-171, 2015.
    Résumé : The levels of sulfate-reducing bacteria (SRB), including Desulfovibrionaceae, in the gut increase following a fat-enriched diet. Endotoxins from gut microbiota contribute to the inflammation process, leading to metabolic diseases. Thus, we sought to characterize the lipid A structures of Desulfovibrionaceae lipopolysaccharides (LPS) that are associated with the microbiota inflammatory properties. LPS variants were obtained from two SRB isolates from the gut of a single individual. These LPS variants shared similar lipid A moieties with Enterobacterial LPS, but differed from one another with regard to fatty-acid numbers and endotoxic activity. This first complete structural characterization of Desulfovibrio lipid A gives new insights into previously published data on Desulfovibrio lipid A biosynthesis. LPS microdiversity within SRBs illustrates how adaptation can influence pro-inflammatory potential.
    Mots-clés : Carbohydrate Conformation, Cytokine, Desulfovibrio desulfuricans, Desulfovibrionaceae, Endotoxin, ESHR, Humans, Inflammation, Intestines, Lipid A, Mass Spectrometry, MICROBIO.
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Publications Principales avant 2015

-  Biofilms formed by gram-negative bacteria undergo increased lipid a palmitoylation, enhancing in vivo survival. Chalabaev S, Chauhan A, Novikov A, Iyer P, Szczesny M, Beloin C, Caroff M, Ghigo JM. MBio. 2014 Aug 19 ;5(4). pii : e01116-14. doi : 10.1128/mBio.01116-14.

-  Essouri S, Laurent M, Chevret L, Durand P, Ecochard E, Gajdos V, Devictor D, Tissières P. Improved clinical and economic outcomes in severe bronchiolitis with pre-emptive nCPAP ventilatory strategy. Intensive Care Med. 2014 Jan ;40(1):84-91.

-  Borckink I, Essouri S, Laurent M, Albers MJ, Burgerhof JG, Tissières P,Kneyber MC. Infants with severe respiratory syncytial virus needed less ventilator time with nasal continuous airways pressure then invasive mechanical ventilation. Acta Paediatr. 2014 Jan ;103(1):81-5.

-  Shah NR, Moksa M, Novikov A, Perry MB, Hirst M, *Caroff M*, Fernandez RC. Draft Genome Sequences of Bordetella hinzii and Bordetella trematum. Genome Announc. 2013 Oct 24 ;1(5).

-  Novikov A, Shah NR, Albitar-Nehme S, Basheer SM, Trento I, Tirsoaga A, Moksa M, Hirst M, Perry MB, Hamidi AE, Fernandez RC, *Caroff M*. Complete Bordetella avium, Bordetella hinzii and Bordetella trematum lipid A structures and genomic sequence analyses of the loci involved in their modifications. Innate Immun. 2013 Oct 14.

-  Albitar-Nehme S, Basheer SM, Njamkepo E, Brisson JR, Guiso N, Caroff M, (2013), Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era, Carbohydr Res. 2013 Aug 30 ;378:56-62

-  Le Moigne V, Le Moigne D, *Mahana W*. Antibody response to Mycobacterium tuberculosis p27-PPE36 antigen in sera of pulmonary tuberculosis patients. Tuberculosis. 2013 Mar ;93(2):189-91.

-  Fallouh H, *Mahana W*. Antibody to heat shock protein 70 (HSP70) inhibits human T-cell lymphoptropic virus type I (HTLV-I) production by transformed rabbit T-cell lines. Toxins (Basel). 2012 Oct ;4(10):768-77.

-  Caroff M and Novikov A, (2011), Micromethods for lipid a isolation and structural characterization, Methods Mol Biol, 739:135-46

-  Chafchaouni‐Moussaoui I, Novikov A, Bhrada F, Perry MB, Filali‐Maltouf Aand Caroff M, (2011), A new rapid and micro‐scale hydrolysis, using triethylamine citrate, for lipopolysaccharide characterization by mass spectrometry, Rapid Comm. Mass Spectrom, 2011, 25:2043-48

-  Basheer SM, Guiso N, Tirsoaga A, Caroff M and Novikov A, (2011), Structural modifications occurring in lipid A of Bordetella bronchiseptica clinical isolates as demonstrated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun Mass Spectrom, 25(8):1075-81

-  Marr N , Novikov A *, Hajjar AM, Caroff M, Fernandez RC, (2010), Variability in the lipid A structure and endotoxicity of Bordetella pertussis Tohama I and mouse challenge strain 18-323, J. Infect.
Dis, 202(12):1897-906

-  Vallet-Gely I, Opota O, Boniface A, Novikov A, Lemaitre B, (2010), A secondary metabolite acting as a signaling molecule controls Pseudomonas entomophila virulence, Cell Microbiol, sous presse

-  Marr N, Hajjar AM, Shah NR, Novikov A, Yam CS, Caroff M and Fernandez RC, (2010), Substitution of the Bordetella pertussis lipid A phosphate groups with glucosamine is required for robust NF-kappaB activation and release of proinflammatory cytokines in cells expressing human but not murine Toll-like receptor 4-MD-2-CD14, Infect Immun, 78(5):2060-9

-  Vallet-Gely I, Novikov A, Augusto L, Liehl P, Bolbach G, Pechy-Tarr M, Cosson P, Keel C, Caroff M and Lemaitre B, (2010), Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production, Appl Environ Microbiol, 76(3):910-21

-  Ciornei CD, Novikov A, Beloin C, Fitting C, Caroff M, Ghigo JM, Cavaillon JM and Adib-Conquy M, (2010), Biofilm-forming Pseudomonas aeruginosa bacteria undergo lipopolysaccharide structural modifications and induce enhanced inflammatory cytokine response in human monocytes, Innate Immun, 16(5):288-301

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