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8 mars 2018: 1 événement

  • Département Biochimie, Biophysique et Biologie Structurale

    Jeudi 8 mars 10:30-12:00 - Hélène Launay - iSCB (Integrative Structural &Chemical Biology), Marseille

    Structural disorder and photosynthesis regulation deciphered using NMR, SAXS and Modelling

    Résumé : Photosynthesis is the only mean of regulation of terrestrial CO2, and its regulation is mediated via protein-protein interactions, post-translational modifications and disorder-to-order transitions. One of the central regulators of the chemical photosynthesis pathway, the Calvin-Benson-Basham cycle, is the conditionally disordered chloroplastic protein CP12. During the day, reducing conditions prevail in the chloroplast and reduced CP12 is intrinsically disordered. During the night, oxidizing conditions prevail in the chloroplast, CP12 is oxidized, and the protein binds to and inhibits to key players of the CBB cycle : glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK). I will present our recent structural investigation of the Chlamydomonas reinhardtii CP12 using NMR, SAXS, molecular modelling, CD, which show that CP12 is a very peculiar intrinsically disordered protein that undergo several disorder-to-order transitions depending on either its redox state or its association with its partner. CP12 is a model conditionally disordered protein, and the molecular description of its disorder-to-order transitions are required to rationalize the fine tuning of photosynthesis and carbon assimilation in micro-algae.

    Lieu : Bibliothèque, Bât. 34 - Bâtiment 34, Campus Gif-sur-Yvette

    En savoir plus : Département Biochimie, Biophysique et Biologie Structurale