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Accueil > Départements > Microbiologie > Michael DUBOW : Génomique et Biodiversité microbienne des biofilms

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pubmed query = (Vergnaud, Gilles[Author - Full]) OR Pourcel, christine[Author - Full]

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  • Associations between Mycobacterium tuberculosis Beijing genotype and drug resistance to four first-line drugs : A survey in China.

    31 décembre 2017, par Liu H, Zhang Y, Liu Z, Liu J, Hauck Y, Liu J, Dong H, Liu J, Zhao X, Lu B, Jiang Y, Vergnaud G, Pourcel C, Wan K
    Related Articles

    Associations between Mycobacterium tuberculosis Beijing genotype and drug resistance to four first-line drugs: A survey in China.

    Front Med. 2017 Dec 29;:

    Authors: Liu H, Zhang Y, Liu Z, Liu J, Hauck Y, Liu J, Dong H, Liu J, Zhao X, Lu B, Jiang Y, Vergnaud G, Pourcel C, Wan K

    Abstract
    Investigations on the genetic diversity of Mycobacterium tuberculosis in China have shown that Beijing genotype strains play a dominant role. To study the association between the M. tuberculosis Beijing genotype and the drug-resistance phenotype, 1286 M. tuberculosis clinical isolates together with epidemiological and clinical information of patients were collected from the center for tuberculosis (TB) prevention and control or TB hospitals in Beijing municipality and nine provinces or autonomous regions in China. Drug resistance testing was conducted on all the isolates to the four first-line anti-TB drugs (isoniazid, rifampicin, streptomycin, and ethambutol). A total of 585 strains were found to be resistant to at least one of the four anti-TB drugs. The Beijing family strains consisted of 499 (53.20%) drug-sensitive strains and 439 (46.80%) drug-resistant strains, whereas the non-Beijing family strains comprised 202 (58.05%) drug-sensitive strains and 146 (41.95%) drug-resistant strains. No significant difference was observed in prevalence (χ2= 2.41, P > 0.05) between the drug-resistant and drugsensitive strains among the Beijing family strains. Analysis of monoresistance, multidrug-resistant TB, and geographic distribution of drug resistance did not find any relationships between the M. tuberculosis Beijing genotype and drug-resistance phenotype in China. Results confirmed that the Beijing genotype, the predominant M. tuberculosis genotype in China, was not associated with drug resistance.

    PMID: 29288283 [PubMed - as supplied by publisher]

  • Correction to : CRISPR-like sequences in Helicobacter pylori and application in genotyping.

    15 décembre 2017, par Bangpanwimon K, Sottisuporn J, Mittraparp-Arthorn P, Ueaphatthanaphanich W, Rattanasupar A, Pourcel C, Vuddhakul V

    Correction to: CRISPR-like sequences in Helicobacter pylori and application in genotyping.

    Gut Pathog. 2017;9:72

    Authors: Bangpanwimon K, Sottisuporn J, Mittraparp-Arthorn P, Ueaphatthanaphanich W, Rattanasupar A, Pourcel C, Vuddhakul V

    Abstract
    [This corrects the article DOI: 10.1186/s13099-017-0215-8.].

    PMID: 29239403 [PubMed - in process]

  • CRISPR-like sequences in Helicobacter pylori and application in genotyping.

    28 novembre 2017, par Bangpanwimon K, Sottisuporn J, Mittraparp-Arthorn P, Ueaphatthanaphanich W, Rattanasupar A, Pourcel C, Vuddhakul V

    CRISPR-like sequences in Helicobacter pylori and application in genotyping.

    Gut Pathog. 2017;9:65

    Authors: Bangpanwimon K, Sottisuporn J, Mittraparp-Arthorn P, Ueaphatthanaphanich W, Rattanasupar A, Pourcel C, Vuddhakul V

    Abstract
    Background: Many bacteria and archaea possess a defense system called clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (CRISPR-Cas system) against invaders such as phages or plasmids. This system has not been demonstrated in Helicobacter pylori. The numbers of spacer in CRISPR array differ among bacterial strains and can be used as a genetic marker for bacterial typing.
    Results: A total of 36 H. pylori isolates were collected from patients in three hospitals located in the central (PBH) and southern (SKH) regions of Thailand. It is of interest that CRISPR-like sequences of this bacterium were detected in vlpC encoded for VacA-like protein C. Virulence genes were investigated and the most pathogenic genotype (cagA vacA s1m1) was detected in 17 out of 29 (58.6%) isolates from PBH and 5 out of 7 (71.4%) from SKH. vapD gene was identified in each one isolate from PBH and SKH. CRISPR-like sequences and virulence genes of 20 isolates of H. pylori obtained in this study were analyzed and CRISPR-virulence typing was constructed and compared to profiles obtained by the random amplification of polymorphic DNA (RAPD) technique. The discriminatory power (DI) of CRISPR-virulence typing was not different from RAPD typing.
    Conclusion: CRISPR-virulence typing in H. pylori is easy and reliable for epidemiology and can be used for inter-laboratory interpretation.

    PMID: 29177012 [PubMed]

  • Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage.

    20 novembre 2017, par Latino L, Pourcel C
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    Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage.

    Methods Mol Biol. 2018;1693:85-98

    Authors: Latino L, Pourcel C

    Abstract
    Bacteria and bacteriophages coexist and coevolve, bacteriophages being obligatory predators exerting an evolutionary pressure on their prey. Mechanisms in action vary depending on the bacterial genomic content and on the regulation of the bacteriophage cycle. To assess the multiplicity of bacterial genes involved in resistance as well as the changes in the bacteriophage interactions with the bacteria, it is necessary to isolate and investigate large numbers of independent resistant variants. Here we describe protocols that have been applied to the study of Pseudomonas aeruginosa and four of its virulent bacteriophages belonging to the Podoviridae and Myoviridae bacteriophage families. Mutations are identified using whole genome sequencing of resistant variants. Phenotypic analyses are performed to describe the changes conferred by the mutations.

    PMID: 29119434 [PubMed - in process]

  • Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica.

    6 septembre 2017, par Timofeev V, Bakhteeva I, Titareva G, Kopylov P, Christiany D, Mokrievich A, Dyatlov I, Vergnaud G

    Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica.

    PLoS One. 2017;12(9):e0183714

    Authors: Timofeev V, Bakhteeva I, Titareva G, Kopylov P, Christiany D, Mokrievich A, Dyatlov I, Vergnaud G

    Abstract
    Francisella tularensis, a small Gram-negative bacterium, is capable of infecting a wide range of animals, including humans, and causes a plague-like disease called tularemia-a highly contagious disease with a high mortality rate. Because of these characteristics, F. tularensis is considered a potential agent of biological terrorism. Currently, F. tularensis is divided into four subspecies, which differ in their virulence and geographic distribution. Two of them, subsp. tularensis (primarily found in North America) and subsp. holarctica (widespread across the Northern Hemisphere), are responsible for tularemia in humans. Subsp. novicida is almost avirulent in humans. The fourth subspecies, subsp. mediasiatica, is the least studied because of its limited distribution and impact in human health. It is found only in sparsely populated regions of Central Asia. In this report, we describe the first focus of naturally circulating F. tularensis subsp. mediasiatica in Russia. We isolated and characterized 18 strains of this subspecies in the Altai region. All strains were highly virulent in mice. The virulence of subsp. mediasiatica in a vaccinated mouse model is intermediate between that of subsp. tularensis and subsp. holarctica. Based on a multiple-locus variable number tandem repeat analysis (MLVA), we show that the Altaic population of F. tularensis subsp. mediasiatica is genetically distinct from the classical Central Asian population, and probably is endemic to Southern Siberia. We propose to subdivide the mediasiatica subspecies into three phylogeographic groups, M.I, M.II and M.III.

    PMID: 28873421 [PubMed - in process]