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Accueil > Départements > Biochimie, Biophysique et Biologie Structurale > Christophe LE CLAINCHE & Louis RENAULT : Dynamique du cytosquelette et motilité

pubmed : ( ( (( "le clainche ...

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NCBI : db=pubmed ; Term=( ( (( "Le clainche C"[author] OR "Renault L"[author] OR "DAVID V"[author] OR "LAI TL"[author] OR "HENRIOT V"[author] OR ("WANG H"[author] AND "Le clainche C"[author]) OR ("FELLOUS S"[author] AND "RENAULT L"[author]) ) AND "2014/01/01"[Date - Publication] : "3000"[Date - Publication] ) OR ( "2014/01/01"[Date - Publication] : "2018/01/01"[Date - Publication] AND ("Carlier MF"[author] OR (("Ciobanasu C"[author] OR "Faivre B"[author]) AND "Le clainche C"[author]) OR (("PERNIER J"[author] OR "MONTAVILLE P"[author] OR "Shekhar S"[author] OR "Kühn S"[author] OR "Guichard B"[author] OR "Jiao Y"[author] OR "COMPPER C"[author]) AND "Carlier MF"[author]) ) ) ) AND ("Laboratoire d’Enzymologie et Biochimie Structurales"[Affiliation] OR I2BC[Affiliation] OR "Institute for Integrative Biology of the Cell"[Affiliation] ) OR ("LAI TL"[author] AND "2016/01/01"[Date - Publication] : "3000"[Date - Publication] AND "iBiTec-s"[Affiliation]) )

Articles syndiqués

  • Enhanced Depolymerization of Actin Filaments by ADF/Cofilin and Monomer Funneling by Capping Protein Cooperate to Accelerate Barbed-End Growth.

    20 juin, par Shekhar S, Carlier MF
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    Enhanced Depolymerization of Actin Filaments by ADF/Cofilin and Monomer Funneling by Capping Protein Cooperate to Accelerate Barbed-End Growth.

    Curr Biol. 2017 Jun 09;:

    Authors: Shekhar S, Carlier MF

    Abstract
    A living cell's ability to assemble actin filaments in intracellular motile processes is directly dependent on the availability of polymerizable actin monomers, which feed polarized filament growth [1, 2]. Continued generation of the monomer pool by filament disassembly is therefore crucial. Disassemblers like actin depolymerizing factor (ADF)/cofilin and filament cappers like capping protein (CP) are essential agonists of motility [3-8], but the exact molecular mechanisms by which they accelerate actin polymerization at the leading edge and filament turnover has been debated for over two decades [9-12]. Whereas filament fragmentation by ADF/cofilin has long been demonstrated by total internal reflection fluorescence (TIRF) [13, 14], filament depolymerization was only inferred from bulk solution assays [15]. Using microfluidics-assisted TIRF microscopy, we provide the first direct visual evidence of ADF's simultaneous severing and rapid depolymerization of individual filaments. Using a conceptually novel assay to directly visualize ADF's effect on a population of pre-assembled filaments, we demonstrate how ADF's enhanced pointed-end depolymerization causes an increase in polymerizable actin monomers, thus promoting faster barbed-end growth. We further reveal that ADF-enhanced depolymerization synergizes with CP's long-predicted "monomer funneling" [16] and leads to skyrocketing of filament growth rates, close to estimated lamellipodial rates. The "funneling model" hypothesized, on thermodynamic grounds, that at high enough extent of capping, the few non-capped filaments transiently grow much faster [15], an effect proposed to be very important for motility. We provide the first direct microscopic evidence of monomer funneling at the scale of individual filaments. These results significantly enhance our understanding of the turnover of cellular actin networks.

    PMID: 28625780 [PubMed - as supplied by publisher]