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  • Regulation of hydroxycinnamic acid degradation drives Agrobacterium fabrum lifestyles.

    22 février, par Meyer T, Renoud S, Vigouroux A, Miomandre A, Gaillard V, Kerzaon I, Prigent-Combaret C, Comte G, Morera S, Vial L, Céline L
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    Regulation of hydroxycinnamic acid degradation drives Agrobacterium fabrum lifestyles.

    Mol Plant Microbe Interact. 2018 Feb 20;:

    Authors: Meyer T, Renoud S, Vigouroux A, Miomandre A, Gaillard V, Kerzaon I, Prigent-Combaret C, Comte G, Morera S, Vial L, Céline L

    Abstract
    Regulatory factors are key components for the transition between different lifestyles to ensure rapid and appropriate gene expression upon perceiving environmental cues. Agrobacterium fabrum C58 (formerly called A. tumefaciens C58) has two contrasting lifestyles: it can interact with plants either as a rhizosphere inhabitant (rhizospheric lifestyle), or as a pathogen that creates its own ecological niche in a plant tumor via its tumor-inducing plasmid (pathogenic lifestyle). Hydroxycinnamic acids are known to play an important role in the pathogenic lifestyle of Agrobacterium but can be degraded in A. fabrum species. We investigated the molecular and ecological mechanisms involved in the regulation of A. fabrum species-specific genes responsible for hydroxycinnamic acid degradation. We characterized the effectors (feruloyl-CoA; p-coumaroyl-CoA) and the DNA targets of the MarR transcriptional repressor ̶ that we named HcaR ̶ which regulates hydroxycinnamic acid degradation. Using an hcaR-deleted strain, we further revealed that hydroxycinnamic acid degradation interfere with vir gene expression. The HcaR deletion mutant shows a contrasting competitive colonization ability, being less abundant than the wild-type strain in tumors, but more abundant in the rhizosphere. This supports the view that A. fabrum C58 HcaR regulation through ferulic and p-coumaric acids perception is important for the transition between lifestyles.

    PMID: 29460677 [PubMed - as supplied by publisher]

  • Targeted profiling of A. thaliana sub-proteomes illuminates new co- and post-translationally N-terminal Myristoylated proteins.

    18 février, par Majeran W, Le Caer JP, Ponnala L, Meinnel T, Giglione C
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    Targeted profiling of A. thaliana sub-proteomes illuminates new co- and post-translationally N-terminal Myristoylated proteins.

    Plant Cell. 2018 Feb 16;:

    Authors: Majeran W, Le Caer JP, Ponnala L, Meinnel T, Giglione C

    Abstract
    N-terminal myristoylation (MYR) is a biologically important co-translational protein lipidation. MYR is difficult to detect in vivo and challenging to predict in silico. We developed a targeted proteomics strategy to identify the Arabidopsis thaliana myristoylated (MYRed) proteome in specific sub-cellular compartments. This global profiling approach allowed us to: (i) identify one third of all open reading frames of the A. thaliana proteome including 54% of the predicted myristoylome; and (ii) establish the first comprehensive plant myristoylome, featuring direct evidence of MYR in 72 proteins. Eighteen MYRed proteins were unexpected, indicating that the in vivo A. thaliana myristoylome extends beyond current predicted sets. A MYR site was also identified downstream of a predicted initiation codon, indicating that post-translational MYR occurs in plants. Over half of the identified proteins could be quantified and assigned to a subcellular compartment. Hierarchical clustering of protein accumulation profiles combined with MYR data and the S-acylated (PALed) proteome revealed that N-terminal double acylation drastically influences protein redirection to the plasma membrane. In a few cases, MYR function extended beyond simple membrane association. This study identified hundreds of N-acylated proteins for which these modifications have the potential to define new protein localization control mechanisms and expand protein function.

    PMID: 29453228 [PubMed - as supplied by publisher]

  • Solid-state [13C-15N] NMR resonance assignment of hepatitis B virus core protein.

    17 février, par Lecoq L, Wang S, Wiegand T, Bressanelli S, Nassal M, Meier BH, Böckmann A
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    Solid-state [13C-15N] NMR resonance assignment of hepatitis B virus core protein.

    Biomol NMR Assign. 2018 Feb 16;:

    Authors: Lecoq L, Wang S, Wiegand T, Bressanelli S, Nassal M, Meier BH, Böckmann A

    Abstract
    Each year, nearly 900,000 deaths are due to serious liver diseases caused by chronic hepatitis B virus infection. The viral particle is composed of an outer envelope and an inner icosahedral nucleocapsid formed by multiple dimers of a ~ 20 kDa self-assembling core protein (Cp). Here we report the solid-state 13C and 15N resonance assignments of the assembly domain, Cp149, of the core protein in its capsid form. A secondary chemical shift analysis of the 140 visible residues suggests an overall alpha-helical three-dimensional fold matching that derived for Cp149 from the X-ray crystallography of the capsid, and from solution-state NMR of the Cp149 dimer. Interestingly, however, at three distinct regions the chemical shifts in solution differ significantly between core proteins in the capsid state versus in the dimer state, strongly suggesting the respective residues to be involved in capsid assembly.

    PMID: 29450824 [PubMed - as supplied by publisher]

  • Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.

    16 février, par Molina-Moya B, Gomgnimbou MK, Spinasse L, Obasanya J, Oladimeji O, Dacombe R, Edwards T, Daragon XO, Lawson L, Abdurrahman ST, Cuevas LE, Dominguez J, Sola C

    Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.

    PLoS Negl Trop Dis. 2018 Feb 15;12(2):e0006242

    Authors: Molina-Moya B, Gomgnimbou MK, Spinasse L, Obasanya J, Oladimeji O, Dacombe R, Edwards T, Daragon XO, Lawson L, Abdurrahman ST, Cuevas LE, Dominguez J, Sola C

    Abstract
    METHODS: All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays.
    RESULTS: Spoligotyping results were obtained for 549 (59.1%) of 929 samples. Lineage 4 Cameroon sublineage (L4.6.2) represented half of the patterns, Mycobacterium africanum (L5 and L6) represented one fifth of the patterns, and all other lineages, including other L4 sublineages, represented one third of the patterns. Sublineage L4.6.2 was mostly identified in the north of the country whereas L5 was mostly observed in the south and L6 was scattered. The spatial distribution of genotypes had genetic geographic gradients. We did not obtain results enabling the detection of drug-resistance mutations.
    CONCLUSION/SIGNIFICANCE: We present the first national snapshot of the M. tuberculosis spoligotypes circulating in Nigeria based on ZN slides. Spoligotyping data can be obtained in a rapid and high-throughput manner with DNA extracted from ZN-stained slides, which may potentially improve our understanding of the genetic epidemiology of TB.

    PMID: 29447161 [PubMed - as supplied by publisher]

  • Coordination between replication, segregation and cell division in multi-chromosomal bacteria : lessons from Vibrio cholerae.

    16 février, par Espinosa E, Barre FX, Galli E

    Coordination between replication, segregation and cell division in multi-chromosomal bacteria: lessons from Vibrio cholerae.

    Int Microbiol. 2017 Sep;20(3):121-129

    Authors: Espinosa E, Barre FX, Galli E

    Abstract
    Bacteria display a highly flexible cell cycle in which cell division can be temporally disconnected from the replication/segregation cycle of their genome. The accuracy of genetic transmission is enforced by restricting the assembly of the cell division apparatus to the low DNA-density zones that develop between the regularly spaced nucleoids originating from the concurrent replication and segregation of genomic DNA. In most bacteria, the process is simplified because the genome is encoded on a single chromosome. This is notably the case in Escherichia coli, the most well studied bacterial model organism. However, ~10% of bacteria have domesticated horizontally acquired mega-plasmids into extra-numerous chromosomes. Most of our current knowledge on the cell cycle regulation of multi-chromosomal species derives from the study of replication, segregation and cell division in Vibrio cholerae, the agent of the deadly epidemic human diarrheal disease cholera. A nicety of this model is that it is closely related to E. coli in the phylogenetic tree of bacteria. Here, we review recent findings on the V. cholerae cell cycle in the context of what was previously known on the E. coli cell cycle.

    PMID: 29446803 [PubMed - in process]

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