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One-year Postdoctoral position
A one-year postdoctoral position funded by DIM1 Health (Région Ile-de France) starting beginning of 2021 is available in the research group "Transcription regulation in the malaria parasite" to work on characterization of a nuclear protein complex from Plasmodium falciparum.
The postdoctoral researcher will work in a project combining parasitology, proteomics and biochemistry, as part of a new ATIP-Avenir team at the Genome Biology department of the I2BC. The goal of the project is to determine how two essential nuclear proteins from the malaria parasite, Plasmodium falciparum, interact to then design protein complex inhibitors. The postdoc will collaborate with two teams, one from the I2BC and one from IPSIT, to reach the project goals.

The I2BC is a University of Paris-Saclay/CNRS/CEA research unit situated in the research campus of Gif-sur-Yvette, 35km southwest of Paris. The campus is in close proximity to a natural park and is deserved by public transportation with direct access to Paris. The institute hosts around 750 people among 60 research teams and 14 state-of-the-art technical facilities.

To apply, please send a letter of motivation, CV and the coordinates of 2 referees to Joana Santos.


Two-years Postdoctoral position

A two-year postdoctoral position funded by the French National Research Agency (ANR) is available at the research group of « Intracellular Trafficking and Organelle Biogenesis of Toxoplasma » in the Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CNRS-CEA, and Paris-Saclay University (South Paris University). Our group is interested in the intracellular trafficking of proteins and lipids that are involved in the biogenesis of secretory organelles known to play key roles in infection of hosts by Toxoplasma gondii. The current project will focus on the functional analyses of newly identified parasite’s proteins that bind to lipids, which are likely involved in the membrane trafficking from the post-Golgi/endosome-like compartement (ELC) to the secretory organelles of T. gondii. By using a combination of approaches including cell biology and biochemistry, we aim to decipher the roles of these novel proteins in the formation of membrane-bound vesicles that are required for secretory organelle biogenesis.

The applicant should have a strong background in cell and molecular biology. Prior experience in imaging (confocal microscopy) analyses and biochemical methods to study protein-protein (IP) will be highly advantageous. The ideal candidate should be highly motivated, curious and will be actively involved in interdisciplinary research with renowned laboratories and core facilities of the Institute for Integrative Biology of the Cell (I2BC). A good level in English (written and spoken) is required. Review of applications will begin immediately until the position filled.

Interested candidates should send by e-mail their CV, a list of two or three references and a cover letter stating their research experience and interests to Dr Stanislas Tomavo




Two-year post-doctoral position
A 2-year post-doc position (funded by ANR Jeune Chercheur Jeune Chercheuse) is available from summer 2020 in the new team of Angélique Déléris to explore the epigenetic regulation of transposable elements in Arabidopsis thaliana in particular after their activation by biotic stress.
The study will use a wide range of technologies in epigenetics, molecular biology and cytology.
The Genome Biology department of I2BC has a long-standing expertise in cell biology, molecular biology, genomics, genetics, biochemistry, and studies a variety of models among which Arabidopsis thaliana. The institute has ten in house technological platforms, including DNA sequencing, bioinformatics, proteomics, imaging, plant culture, etc....It is located within the campus of CNRS in Gif-sur-Yvette, about 45 minutes south of Paris.
We are looking for independent researchers with good communication skills, who are open minded, and with a good team spirit. A strong background in molecular biology techniques is required ; a background in plant epigenetics or gene regulation is preferred. Experience with cell imaging would be helpful, but is not required. A computational background (or willingness to learn basic computational methods) is also a plus.
This offer will appear formerly in CNRS Job news at https://emploi.cnrs.fr.
Application, including a detailed CV, a brief summary of research interests, and names and addresses of two referees, could already be addressed by mail to Angélique Déléris




12 months Engineer Assistant position available at I2BC, Gif Sur Yvette (20 km from Paris- FRANCE)
A 12 months engineer assistant position is available starting beginning of 2020 to study the plastid machinery involved in RbcL processing in the group of “Protein Maturation Cell Fate and Therapeutics” at the I2BC in Gif Sur Yvette (https://www.i2bc.paris-saclay.fr/spip.php?article1).


Background
Chloroplasts (Cp) evolved from engulfed prokaryotes, most likely cyanobacteria that once lived as independent organisms. Thus, for many aspects chloroplasts are much more similar to bacteria. Nonetheless, the new cellular resident quickly evolved acquiring original and unique features as the lost or relocation of the majority of the genes to the nucleus of the host while only about 80- protein encoding genes remained in the organelle.
All nuclear and plastid-encoded chloroplast-localized proteins undergo many co- and post-translational modifications (CTMs and PTMs). This implies that both types of modifications may provide a substantial control for stability, accumulation, activity, assembly, and compartmentalisation. However, CTMs and PTMs of plastid proteins and their catalytic modifiers have not intensively been explored up to date. This particularly holds true for N-terminal Protein Modifications (NPMs).
The earliest NPM is the essential N-terminal methionine excision (NME). NME corresponds to the removal of the first amino acid incorporated in any nascent peptide chain (iMet). In plastids, MetAPs, the specific modifiers responsible for iMet excision, ensure NME. Still, a number of exceptions to the established NME rules exist in plastid, suggesting that pMetAPs might function differently from previously characterized MetAPs. Of note, the most unusual, intricate maturation mechanism of the N-ter of one of the subunits (RbcL) of the enzyme RuBisCO, which is the major component of the Calvin-Benson cycle ans the most abundant protein on earth, is a conundrum for decades as well as is its implication on RubisCO activity, assembly, localization or interaction with partners.


Project
The goal of this one-year project is the elucidation of the unique plastid machinery involved in RbcL processing. For this project we are looking for an Engineer Assistant with strong expertise in molecular biology, protein biochemistry and enzymology. Experience in mass spectrometry targeted techniques, enrichment of post-translational modification will be appreciated. The selected person will be in charge of the biochemical characterization of NPM modifiers and analysis of the impact of NPMs on RuBiscCO in E. coli. The hired person will be trained to use our in-house pipelines/methods for the proteomics N-terminal characterization and quantification, NAT and MetAP enzymatic evaluation and partners sample analyses by MS. The successful candidate should be able to perform autonomously the experiments, analyze and interpret the emerging data. The ideal candidate is expected to be highly motivated and capable to have a strong relational and organizational sense. Fluency in English is preferred.


For further information please contact :
Carmela GIGLIONE

Carmela Giglione PhD
Protein Maturation, Cell fate and Therapeutics
Institute for Integrative Biology of the Cell (I2BC)
CNRS UMR9198, Bâtiment 21, 1 avenue de la Terrasse
F-91198 Gif-sur-Yvette cedex, France
E-mail : carmela.giglione i2bc.paris-saclay.fr
Tel : 01 69 82 46 44
http://www.i2bc.paris-saclay.fr/spip.php?article130


To apply it is required :
• A CV
• Full Contact details of at least two references


Selected Publications of the group :
- Castrec B, et al (2018) Structural and genomic decoding of human and plant myristoylomes reveals a definitive recognition pattern. Nat Chem Biol 14 : June 11, DOI : 10.1038/s41589-018-0077-5
- Majeran W, et al (2018) Targeted profiling of A. thaliana sub-proteomes illuminates new co- and post-translationally N-terminal Myristoylated proteins. Plant Cell 30 : 543–562
- Bienvenut WV, Giglione C, Meinnel T (2015) Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state and upon deformylation inhibition. Proteomics 15 : 2503-18
- Bienvenut WV, Giglione C, Meinnel T (2017) SILProNAQ : A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation. Methods Mol Biol 1574 : 17-34
- Bienvenut WV, et al (2017) EnCOUNTer : a parsing tool to uncover the mature N-terminus of organelle-targeted proteins in complex samples. BMC Bioinformatics 18 : 182
- Linster E., et al (2015) Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun 6 : 7640
Xu F., et al (2015) Two N-terminal acetyltransferases antagonistically regulate the stability of a nod-like receptor in Arabidopsis. Plant Cell 27 : 1547-62
- Dinh, T.V et al (2015) Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by a global acetylome profiling test, Proteomics, 15, 2426-2435
- Bienvenut, W.V., et al (2012) Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features. Mol. Cell. Proteomics, 11 : M111 015131



There are no other open position in the Institute at the moment.

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