Agenda
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Lundi 2 juin 2014 14:00-15:30 - Taekjip Ha - Université de physique de l'Illinois Surprising physics of DNA and its implication in gene regulation Résumé : Summary : Using advanced single molecule measurements techniques and all atom computational simulations, we discovered two interesting physical properties of DNA. First, DNA sequence and modifications have profound influence on DNA flexibility which in turn determines the asymmetry in mechanical stability of nucleosomes and the accessibility of nucleosomal DNA. Second, DNA sequence and modifications strongly influence association between double stranded DNA molecules without Watson-Crick basepairing, thereby affecting chromosomal organizations.
Lieu : Bâtiment 21, auditorium - CNRS de Gif sur Yvette
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Vendredi 6 juin 2014 14:00-15:30 - Yves Barral - Institut de biochimie de l'ETH ZURICH Genomic immunity : How do eukaryotes protect themselves against the propagation of foreign DNA molecules ? Lieu : Bâtiment 21, auditorium - CNRS de Gif sur Yvette
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Jeudi 12 juin 2014 10:00-11:30 - Niko Hildebrandt Luminescence and FRET : Time-resolved spectroscopy and imaging for sensitive multiplexed biosensing Résumé : Summary :
"Applications based on Förster Resonance Energy Transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of the life sciences. Using time-resolved optical spectroscopy and microscopy for the analysis of FRET systems offers several advantages concerning sensitivity and specificity. Two extraordinary materials concerning time-resolved FRET are luminescent lanthanide complexes (LLCs) and semiconductor quantum dots (QDs). Both are frequently used as FRET donors in combination with organic dyes, fluorescent proteins and other fluorophores. However, their combination as LLC-QD donor-acceptor pair gives access to unique optical properties, which make this FRET-pair an incredible tool for highly sensitive multiplexed diagnostics.
The presentation will start with an introduction to FRET, lanthanide complexes and quantum dots and their possible applications. Then it will focus on recent research results concerning FRET for ultrasensitive multiplexed biosensing applications such as homogeneous immunoassays, microRNA detection, enzyme kinetics, molecular rulers, epithelial-mesenchymal transition, molecular logic gates, and crossing of the blood-brain barrier."Lieu : Salle de conférence George Prevost ISV/CGM - Campus de Gif sur Yvette
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Lundi 1er septembre 2014 14:30-15:30 - Marina Rodnina Co-translational protein folding on the ribosome Résumé : Marina Rodnina : "Co-translational protein folding on the ribosome
Lieu : Auditorium bâtiment 21 - Campus CNRS de Gif/Yvette
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Lundi 1er septembre 2014 15:30-16:30 - Wolfgang Wintermeyer Interplay between protein biogenesis factors at the peptide exit of the ribosome Lieu : Auditorium bâtiment 21 - Campus CNRS de Gif/Yvette
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Vendredi 5 septembre 2014 10:30-12:30 - Franck Perez Rushing to study Golgi-dependent protein secretion in mammalian cells Lieu : Auditorium bâtiment 21 - Campus CNRS de Gif/Yvette
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Mardi 30 septembre 2014 11:00-13:00 - Annemarie Wehenkel Understanding the molecular mechanisms of tubulin heterogeneity Lieu : Auditorium bâtiment 21 - Campus CNRS de Gif/Yvette
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Mardi 7 octobre 2014 14:00-15:00 - Xavier Darzacq Dissecting transcription regulation using single molecule imaging Lieu : Auditorium bâtiment 21 - Campus CNRS de Gif/Yvette
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Vendredi 21 novembre 2014 11:00-12:00 - Cristina Risco - Centro Nacional de Biotecnología, Madrid Studying viral infections and cell architecture with new imaging techniques Résumé : Replication and assembly of many RNA viruses take place in specific intracellular compartments known as ‘virus factories’, ‘viral inclusions’ or ‘viroplasms’. Our knowledge of the biogenesis and architecture of these unique structures has increased considerably in the last few years, due to technical advances in cellular, molecular and structural biology. Early in infection, viral polymerases and cofactors interact with cell membranes, where they build functional replication complexes (RC) and replication organelles. These structures recruit viral and cell factors to build a platform on which viral replication and morphogenesis are spatially connected. Factories are very dynamic, as their structure changes over time. A number of imaging technologies are helping us to understand how factories are built and work. 3D imaging shows the complex organization of virus factories, in particular the new interactions built between virus-induced membranous scaffolds, cytoskeleton and recruited organelles. A summary of recent findings on the structure of factories will be presented, including 3D reconstructions of serial sections, electron tomography and molecular mapping with antibodies and with the new clonable tag for electron microscopy based on the small metal-binding protein metallothionein (MT). Study of the intracellular distribution of MT-tagged viral replicase proteins revealed virus-induced structures not previously observed. Designing strategies to combine functional and mechanistic studies with 3D imaging will be necessary to understand the meaning of the structures built by viruses in the infected cell.Retour ligne automatique
Fernández de Castro, I., Volonte, l., Risco, C. Virus factories : biogenesis and structural design. Cell Microbiol, 2013. 15 : 24-34.
Barajas, D., Fernández de Castro, I., Pogany, J., Risco, C., & Nagy, P.D. Noncanonical role for the host Vps4 AAA+ ATPase ESCRT protein in the formation of Tomato bushy stunt virus replicase. PLoS Pathog, 2014. 10(4) : e1004087.
Fernández de Castro, I., Zamora, P.F., Ooms, L., Fernández, J.J., Lai, C.M.-H., Mainou, B.A., Dermody, T.S., & Risco, C. Reovirus forms neoorganelles for progeny assembly within reorganized cell membranes. mBio, 2014. 5:e00931-13.
Risco, C., Fernández de Castro, I., Sanz-Sánchez, L., Narayan, K., Grandinetti, G., Subramaniam, S. 3D imaging of viral infections. Annu Rev Virol, 2014. 1:453-473.
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Invité par Yves Gaudin (Virologie)Lieu : Amphitéâtre Bâtiment 21, Campus Gif
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Vendredi 25 septembre 2015 - I2BC -MICALIS – VIM Première rencontre I2BC -MICALIS – VIM Lieu : Auditorium Bâtiment 21 - Campus CNRS
Avenue de la terrasse
Gif Sur Yvette -
Du 5 octobre 2016 14:00 au 7 octobre 2016 13:00 - Journées Scientiques de l’I2BC Résumé : Nous recevons Jörg Vogel, Eriko Takano, Harald Stenmark et Jordan Raff.
L’inscription est obligatoire et la clôture des inscriptions aura lieu le 18 septembre sur le site du colloque (lien)
Il est encore possible de proposer des posters en suivant les recommandations (pdf)
Nous espérons vous voir nombreux !
Le comité d’organisationLieu : Auditorium - Bâtiment 21, Campus de Gif
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Mercredi 18 janvier 2017 09:00-17:00 - BIG/3D_CHROME meeting Résumé : Click to download the program
09:00 - 09:15 Welcome (F Boccard)
Research projects supported by the BIG Lidex
09:15 - 09:35 Tamara Basta-Leberre (BIG project coordinator, I2BC)
Structure, mechanism and evolution of the universal tRNA
threonylcarbamoylation systems
09:35 - 09:55 Maria Costa (BIG project coordinator, I2BC)
Bacterial group II introns targeting translational punctuation signals :
mechanisms and implications
09:55 - 10:15 Daniel Gautheret (BIG project coordinator, I2BC)
The bizarre transcriptome of RNA processing mutants : lessons from AGO2 and
CPEB1
10 : 15 - 10:35 Carmela Giglione (BIG project coordinator, I2BC)
Proteogenomic analysis to monitor N-terminal cotranslational protein
maturation in vivo
10:35 - 10:55 Coffee break
10:55 - 11:20 Yoshiharu Yamaichi (BIG project coordinator, I2BC) and Ipek Altinoglu (BIG PhD student, Yamaichi lab, I2BC)
Organization of bacterial cells
11:20 - 11:35 Florian Carlier (BIG PhD student, Malagnac lab, I2BC)
Are RIP and heterochromatin involved in Podospora anserina sexual
development ?
11:35 - 11:50 Charbel Souaid (BIG PhD student, Noordermeer lab, I2BC)
Identification and functional characterization of constitutive gene-enhancer loops
in mouse embryonic stem cells
11:50 - 12:05 Ganna Kychygina (BIG PhD student, Crabbé lab, I2BC)
Connection between nuclear envelope dysfunction and DNA damage response at
telomeres
12:05 - 13:30 lunch
Technological projects supported by BIG - connection with 3D_CHROME
13:30 - 13:50 Dominique Fourmy (BIG project coordinator, I2BC)
Fluorescence microscopy : from single molecule dynamics to live cell imaging
13:50 - 14:10 Romain Le Bars (Imagerie Gif platform, I2BC)
PALM & dSTORM : from single molecule localization to super-resolution imaging
and much more
14:10 - 14:30 Laure Crabbé (I2BC, 3D_CHROME)
Super-resolution microscopy to follow human telomeres dynamics in live cells
14:30 - 15:00 Claude Thermes and Daan Noordermeer (I2BC, 3D_CHROME)
New genomics approaches incorporating Nanopore Sequencing at the I2BC
15:00 - 15:20 Valérie Gaudin (IJPB, 3D_CHROME)
Spatial modeling of nuclear organization in plants
15:20 - 15:45 Coffee break
15:45 - 17:00 3D_CHROME business meeting (chair : M Bétermier)
Click to download the programLieu : Auditorium - Building 21, CNRS campus, Gif-sur-Yvette
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Lundi 30 janvier 2017 09:00-18:00 - Recombination and Epigenetics : Symposium in memory of Jean-Luc Rossignol Résumé : Un colloque est organisé le 30 janvier 2017 en hommage au Pr Jean Luc Rossignol à l’initiative de la Société Française de Génétique. Vous trouverez joint l’affiche, les modalités pratiques et le programme de cette journée. Ce colloque est gratuit mais il est recommandé de s’inscrire.
site d’inscription
Programme
Affiche
Lieu : Amphithéâtre Buffon, Université Paris-Diderot - 15 rue Hélène Brion 75013 Paris
Accès : RER C, Métro ligne 14, Bus n°62, 64, 89 et 325 :arrêt Bibliothèque François Mitterrand -
Vendredi 8 décembre 2017 09:00-17:00 - YOUR-Carreer day Lieu : Auditorium - Bâtiment 21, campus de Gif
