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Offre d’emploi Ingénieur en CDD au département B3S de l’I2BC

Un contrat à durée déterminée, niveau assistant ingénieur ou ingénieur d’études, de 30mois est proposé au sein du laboratoire de Biologie Structurale et Radiobiologie (I2BC, CEA, Saclay). L’équipe étudie la structure et la fonction de protéines et de complexes impliquées dans la maintenance de l’intégrité des génomes eucaryotes (Site de l’équipe)

Le poste proposé comporte deux missions. La première mission porte sur la mesure d’interactions protéines-ligands par la méthode du switchSense ( Le(la) candidate prendra en charge la mesure d’interactions de protéines pour le laboratoire et pour certains projets soumis à cette plateforme. Il formera également les utilisateurs à cette nouvelle technique.
L’autre mission s’insère dans le cadre d’un projet du laboratoire sur les mécanismes moléculaires de réparation des cassures double-brin de l’ADN chez l’homme par la voie NHEJ. Le travail portera sur la purification de protéines et de complexes de cette voie de réparation à des fins d’analyses structurales ou biochimiques. Une partie du travail portera également sur l’analyse qualitative des échantillons purifiés (gel filtration, SEC-MALS) et sur la mesure d’interactions protéine-protéine. Enfin, un dernier volet portera sur la cristallisation des protéines purifiées et l’analyse des cristaux au Synchrotron SOLEIL.

Le profil recherché en priorité est un ou une candidate avec une License ou un Master professionnel. Une première expérience professionnelle de 1-2ans dans un laboratoire de Biochimie ou de Biologie Structurale est un plus important. Une bonne connaissance des méthodes de sur-expression des protéines et des méthodes de chromatographie est souhaitée. L’équipe est située sur le site du CEA de Saclay. L’équipe dispose d’équipements complets et récents pour la production des protéines (Infors, Centrifugeuse, 6 systèmes Akta Purifier/Pure). L’équipe est associée à la plateforme de mesures des interactions des protéines de l’I2BC.

Envoyer un CV, une lettre de motivation et les coordonnées de 2 personnes pouvant servir de référant.

Contact Jean-Baptiste Charbonnier
Laboratoire de Biologie Structurale et Radiobiologie
I2BC, UMR 9198, CEA-Saclay,
91191 Gif-s-Yvette Cedex
tel 01 69 08 76 77

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Offre d’emploi Post-Doctoral au département Virologie de l’I2BC
A 3 years post-doc position granted by ANRS is available starting from January 2019.
The post-doc will join a dynamic research team from the Virology dpt. focusing on virus-host interactions in particular cellular degradation compartments such as autophagy. The group of A. Moris is currently at CIMI-Paris but will move to I2BC in 2019 to dissect the pathways leading to viral antigen presentation by MHC molecules to human T lymphocytes.

The postdoc will characterize the various steps leading to MHC-II-restricted antigen presentation by B cells, from antigen capture mediated by the B-cell-receptor to MHC-II loading compartments and the quality of T cell activation. The project includes the development and characterization of novel libraries of antigen specific human B cells. Our aim is to define the checkpoints and rate-limiting steps of antigen presentation by B cells to help designing novel vaccine strategies that will sustain B-cell-T-cell antigen-specific interactions and thus the generation of broadly Neutralizing Antibodies.
The team has tight contacts with researchers and clinicians involved in HIV research and in charge of patient cohorts. The post doc salary is granted by the French AIDS agency (ANRS) as part of a collaborative work with Hugo Mouquet from the Pasteur Institute.

The candidate
We are interested in rigorous and multi-talented candidates who are enthusiastic and passionate about tackling basic biological questions with potential application in immunotherapy. The candidate should have a PhD in Immunology or Cellular Biology. This position is particularly suited for applicants with a strong background in the field of B cell biology. However, candidates with an expertise in cell biology (e.g. culture of primary human cells, vesicular trafficking and imaging) are also highly encouraged to apply.
Competences in basic molecular biology technics will be appreciated. The candidate should be strongly motivated to develop an innovative project in the field of infectious diseases with a focus on HIV research. The applicant must demonstrate a capacity to rapidly adapt to a new environment ; to show initiative in interacting with colleagues from the team, the dpt, the core facilities and I2BC.

Applications : Candidates should send a motivation letter, CV and contacts of 3 references to Arnaud Moris
Starting date : The position is opened from January 2019.

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Offre d’emploi CDD assistant- ingénieur à l’I2BC
A 30 months engineer assistant position is available at the beginning of December 2018 /January 2019 to study the multifaceted functions of protein acetyltransferases in plant stress response and regulation of metabolism in the group of “Protein Maturation Cell Fate and Therapeutics”.

Protein modifications are emerging as key players for controlling countless essential cellular processes. In principle, all proteins of any living organism undergo to co- and/or post-translational modifications which define protein life and function. Each modification converts one protein species into another that is a functionally distinct species. Among protein modifications, N-terminal protein acetylation (NTA) is one of the most common protein modifications as it occurs on more than 80% of eukaryotic proteins. NTA was initially considered only co-translational, but modification has now been documented also to occur post-translationally after removal of transit peptides from imported proteins in organelles (chloroplast and mitochondria). Despite it has been shown that this modification is involved in a number of crucial cellular processes, controlling in specific proteins half-life, membrane and compartment localization, protein-protein interaction, protein folding, protein aggregation, many questions about this modification and the enzymes involved (Nats) remain to elucidate.

In the course of a recent international financed project, bringing together five recognized European groups (ERACAPS KATNAT), we have started a study which overarching aim is to provide a mechanistic understanding of protein acetylation in plants, with a particular focus on investigating the enzymes that catalyze this modification and its role on proteostasis, photosynthesis and metabolism. For this project we are looking for an Engineer Assistant with expertise in protein biochemistry and enzymology, mass spectrometry targeted techniques, enrichment of post-translational modification. Experience in computational proteomics will be appreciated. The selected person will be trained to use our in-house pipelines/methods for the proteomics N-terminal characterization and quantification, NAT enzymatic evaluation and partners sample analyses by MS. The successful candidate should be able to perform the experiments, analyze and interpret the emerging data. The ideal candidate is expected to be highly motivated and capable to have a strong relational and organizational sense. Fluency in English is preferred.

Applications : a CV and full contact information for at least 2 references should be sent to Carmela GIGLIONE
Starting date : December 2018 / January 2019.

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Offre d’emploi CDD technicien ou ingénieur à l’I2BC

Position on Arabidopsis molecular genetics
A position is available at the Institute for Integrative Biology of the Cell in Gif-sur-Yvette, I2BC ( This position is funded in the context of the project “Improving Seed Iron STORage” (ISISTOR) funded by the ANR. The position is open for technicians or engineers for a duration of 12 months starting in October 2018.

Topic and missions
Seeds contain all the nutrients required for the development of a new organism, including iron which is frequently limiting in the human diet. Seeds are most often the edible part of crops. The objective of the project is to identify novel genes involved in the control of iron storage and remobilisation in seeds using a genetic approach. The candidate will be involved in the identification and characterization of Arabidopsis thaliana mutants : plant culture under controlled conditions, analysis of NGS data, construction of plant transformation vector, Arabidopsis transformation, genotyping and physiological analyses. The goal of this research is to identify candidate genes to improve iron availability in crop seeds.

The candidate should have a training in molecular biology. Experience in plant in vitro culture, Arabidopsis transformation and NGS data analysis will be appreciated. The candidate should be highly motivated and ready to interact within an international team.

Salary : according to experience and degree.
Starting date : from October to December 2018.
Applications : a CV, a statement of interest for the project and contact information for 2 references should be sent to Sébastien THOMINE before September 15th, 2018.

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Nous n’avons actuellement aucune autre offre d’emploi à vous proposer.
Vous pouvez toutefois joindre directement la personne identifiée comme contact dans l’équipe qui vous intéresse si vous souhaitez déposer une candidature spontanée.

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