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Accueil > Départements > Biochimie, Biophysique et Biologie Structurale > Benoit GIGANT & Julie MENETREY : Biochimie Structurale des Microtubules, des Kinésines et de leurs Cargos

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JPEGStructural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif

PubMed : Vilela F, Velours C, Chenon M, Aumont-Nicaise M, Campanacci V, Thureau A, Pylypenko O, Andreani J, Llinas P, Ménétrey J. (2019) Sci Rep. 9(1):16036.


JPEG - 85.5 ko

Insight into microtubule nucleation from tubulin-capping proteins.

PubMed : Campanacci V, Urvoas A, Cantos-Fernandes S, Aumont-Nicaise M, Arteni AA, Velours C, Valerio-Lepiniec M, Dreier B, Plückthun A, Pilon A, Poüs C, Minard P, Gigant B. (2019) Proc Natl Acad Sci U S A. (20) 9859-9864.

PDB : 6GX7, 6GVM and 6GVN

Selection and Characterization of Artificial Proteins Targeting the Tubulin α Subunit.

PubMed : Campanacci V, Urvoas A, Consolati T, Cantos-Fernandes S, Aumont-Nicaise M, Valerio-Lepiniec M, Surrey T, Minard P, Gigant B. (2019) Structure. 27(3):497-506.e4.

PDB : 6GWC and 6GWD

Characterization of the binding mode of JNK-interacting protein 1 (JIP1) to kinesin-light chain 1 (KLC1).

PubMed : Nguyen TQ, Aumont-Niçaise M, Andreani J, Velours C, Chenon M, Vilela F, Geneste C, Varela PF, Llinas P, Ménétrey J. (2018) J Biol Chem. 293(36):13946-13960.

Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain.

PubMed : Nguyen TQ, Chenon M, Vilela F, Velours C, Aumont-Nicaise M, Andreani J, Varela PF, Llinas P, Ménétrey J. (2017) PLoS One. 12(10):e0186354.

PDB : 5OJ8 and 5OJF


Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin.

PubMed : Wang W, Cantos-Fernandes S, Lv Y, Kuerban H, Ahmad S, Wang C, Gigant B. (2017) Nat Comm. 8:70.


The structural switch of nucleotide-free kinesin.

PubMed : Cao L, Cantos-Fernandes S, Gigant B. (2017) Sci Rep. 7:42558.

Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin.

PubMed : Ahmad S, Pecqueur L, Dreier B, Hamdane D, Aumont-Nicaise M, Plückthun A, Knossow M, Gigant B. (2016) Sci Rep. 6:28922.

Structure of a truncated form of the Leucine Zipper II of JIP3 reveals an unexpected anti-parallel coiled-coil arrangement.

PubMed : Llinas, P, Chenon, M, Nguyen, TQ, Moreira, C, de Régibus, A, Coquard, A, Ramos, MJ, Guerois, R, Fernandes, PA, Ménétrey, J (2016) Acta Cryst F72.


Apo-kinesin bound to tubulin.

PubMed : Cao L, Wang W, Jiang Q, Wang C, Knossow M, Gigant B. The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement. (2014) Nat Commun. 5:5364.



Structure of the tubulin-interacting protein CopN from Chlamydia pneumoniae.

PubMed : Nawrotek A, Guimarães BG, Velours C, Subtil A, Knossow M, Gigant B. Biochemical and structural insights into microtubule perturbation by CopN from Chlamydia pneumoniae. (2014) J Biol Chem. 289(36):25199-210.

PDB : 4P3Z


Structure of a stathmin-like domain (magenta) that binds 4 αβ-tubulin molecules.

PubMed : Mignot I, Pecqueur L, Dorléans A, Karuppasamy M, Ravelli RB, Dreier B, Plückthun A, Knossow M, Gigant B. Design and characterization of modular scaffolds for tubulin assembly. (2012) J Biol Chem. 287(37):31085-94.

PDB : 4F61

A tubulin:DARPin complex.

PubMed : Pecqueur L, Duellberg C, Dreier B, Jiang Q, Wang C, Plückthun A, Surrey T, Gigant B, Knossow M. A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. (2012) Proc Natl Acad Sci U S A. 109(30):12011-6.


A tubulin:stathmin-like domain complex with bound colchicine (yellow) and ustiloxin D (green).

PubMed : Ranaivoson FM, Gigant B, Berritt S, Joullié M, Knossow M. Structural plasticity of tubulin assembly probed by vinca-domain ligands. (2012) Acta Crystallogr D Biol Crystallogr. 68(Pt 8):927-34.

PDB : 3UT5

The structural switch of tubulin upon GTP binding.

PubMed : Nawrotek A, Knossow M, Gigant B. The determinants that govern microtubule assembly from the atomic structure of GTP-tubulin. (2011) J Mol Biol. 412(1):35-42.

PDB : 3RYF and 3RYI

The structural basis of Arf effector specificity : the crystal structure of ARF6 in a complex with JIP4.

PubMed : Isabet, T, Montagnac, G, Regazzoni, K, Khadali, El, F, Franco, M, Chavrier, P, Houdusse, A, Ménétrey, J (2009). Embo J 28, 2835–2845.

PDB : 2W83


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