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Accueil > Départements > Biologie des Génomes > Robert DEBUCHY & Fabienne MALAGNAC : Différenciation sexuée et méiose chez les champignons

Publications thématique Stabilité génomique, épigénétique et reproduction sexuée


  • H. Lalucque, F. Malagnac, K. Green, V. Gautier, P. Grognet, L. Chan Ho Tong, B. Scott, et P. Silar, « IDC2 and IDC3, two genes involved in cell non-autonomous signaling of fruiting body development in the model fungus Podospora anserina », Developmental Biology, vol. 421, nᵒ 2, p. 126-138, janv. 2017.
    Résumé : Filamentous ascomycetes produce complex multicellular structures during sexual reproduction. Little is known about the genetic pathways enabling the construction of such structures. Here, with a combination of classical and reverse genetic methods, as well as genetic mosaic and graft analyses, we identify and provide evidence for key roles for two genes during the formation of perithecia, the sexual fruiting bodies, of the filamentous fungus Podospora anserina. Data indicate that the proteins coded by these two genes function cell-non-autonomously and that their activity depends upon conserved cysteines, making them good candidate for being involved in the transmission of a reactive oxygen species (ROS) signal generated by the PaNox1 NADPH oxidase inside the maturing fruiting body towards the PaMpk1 MAP kinase, which is located inside the underlying mycelium, in which nutrients are stored. These data provide important new insights to our understanding of how fungi build multicellular structures.
    Mots-clés : DBG, Developmental mutants, DSMC, Fungal development, Multicellular fruiting bodies, Perithecium, Podospora anserina.



  • A. Goarin, P. Silar, et F. Malagnac, « Gene replacement in Penicillium roqueforti », Current Genetics, vol. 61, nᵒ 2, p. 203-210, mai 2015.
    Résumé : Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologous recombination in P. roqueforti, demonstrating that knockout experiments can be performed in this fungus. To do so, we improved the existing transformation method to integrate transgenes into P. roqueforti genome. In the meantime, we cloned the PrNiaD gene, which encodes a NADPH-dependent nitrate reductase that reduces nitrate to nitrite. Then, we performed a deletion of the PrNiaD gene from P. roqueforti strain AGO. The ΔPrNiaD mutant strain is more resistant to chlorate-containing medium than the wild-type strain, but did not grow on nitrate-containing medium. Because genomic data are now available, we believe that generating selective deletions of candidate genes will be a key step to open the way for a comprehensive exploration of gene function in P. roqueforti.
    Mots-clés : Cheese, DBG, DSMC, Gene Knockout Techniques, Genetic Engineering, Homologous Recombination, Humans, Nitrate Reductase (NADPH), Penicillium.
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Publications Principales avant 2015

- Grognet P, Lalucque H, Malagnac F, Silar P. (2014) Genes that bias mendelian segregation.
PLoS Genet May 15 ;10(5):e1004387. doi : 10.1371/journal.pgen.1004387. eCollection 2014 May.

- Grognet, P., Bidard, F., Kuchly, C., Chan Ho Tong, L., Coppin, E., Benkhali, J.A., Couloux, A., Wincker, P., Debuchy, R., and Silar, P. (2014). Maintaining Two Mating Types : Structure of the Mating Type Locus and Its Role in Heterokaryosis in Podospora anserina. Genetics 197, 421–432.

- Cheeseman K, Ropars J, Renault P, Dupont J, Gouzy J, Branca A, Abraham AL, Ceppi M, Conseiller E, Debuchy R, Malagnac F, Goarin A, Silar P, Lacoste S, Sallet E, Bensimon A, Giraud T, Brygoo Y. (2014) Multiple recent horizontal transfers of a large genomic region in cheese making fungi.
Nat Commun. Jan 10 ;5:2876. doi : 10.1038/ncomms3876.

- Philippe Silar & Fabienne Malagnac
Les champignons redécouverts
Belin, DL 2013, cop. 2013
ISBN 978-2-7011-5902-7
EAN 9782701159027

- Malagnac F, Fabret C, Prigent M, Rousset JP, Namy O, Silar P. (2013) Rab-GDI Complex Dissociation Factor Expressed through Translational Frameshifting in Filamentous Ascomycetes.
PLoS One Sep 19 ;8(9):e73772. doi : 10.1371/journal.pone.0073772.

- Ropars J, Dupont J, Fontanillas E, Rodriguez de la Vega RC, Malagnac F, Coton M, Giraud T and Lopez-Villavicencio M. (2012) Sex in Cheese : Evidence for Sexuality in the Fungus Penicillium roqueforti.
PLoS ONE 7(11) : e49665. doi:10.1371/journal.pone.0049665

- Déquard-Chablat M, Nguyen T-T, Contamine V, Hermann-Le Denmat S and Malagnac F. (2012) Efficient tools to target DNA to Podospora anserina.
Fungal Genetics Reports 59 : 21-25.

- Lalucque H, Malagnac F, Brun S, Kicka S, Silar P. (2012) A Non-Mendelian MAPK-Generated Hereditary Unit Controlled by a Second MAPK Pathway in Podospora anserina.
Genetics. Jun 191(2):419-33.

- Espagne E, Vasnier C, Storlazzi A, Kleckner NE, Silar P, Zickler D, Malagnac F. (2011) Sme4 coiled-coil protein mediates synaptonemal complex assembly, recombinosome relocalization, and spindle pole body morphogenesis.
Proc Natl Acad Sci U S A. 108(26):10614-9.

- Malagnac F. and Silar P.
Epigenetics of Eukaryotic Microbes.
“Handbook of Epigenetics : The New Molecular and Medical Genetics”.
Academic Press. SEP-2010. ISBN 13 : 978-0-12-375709-8.

- Lalucque H., Malagnac F. and Silar P.
Prions and prion-like phenomena in epigenetic inheritance.
“Handbook of Epigenetics : The New Molecular and Medical Genetics”.
Academic Press. SEP-2010. ISBN 13 : 978-0-12-375709-8.

- Brun S., Malagnac F., Bidard F., Lalucque H. and Silar P. (2009) Functions and regulation of the Nox family in the filamentous fungus Podospora anserina : a new role in cellulose degradation.
Mol Microbiol. 74(2):480-96.

- Martins M, Rodrigues-Lima F, Dairou J, Lamouri A, Malagnac F, Silar P, Dupret JM.
An acetyltransferase conferring tolerance to toxic aromatic amine chemicals : molecular and functional studies.
J Biol Chem. (2009) 284(28):18726-33.

- Malagnac F., Bidard F., Lalucque H., Brun S., Lambou K., Lebrun M-H. and Silar P. (2008) Convergent evolution of morphogenetic processes in fungi : Role of tetraspanins and NADPH oxidases 2 in plant pathogens and saprobes.
Commun & Integr Biol. 2:180-181.

- Lambou K, Malagnac F, Barbisan C, Tharreau D, Marc-Henri Lebrun, and Silar P. (2008) The crucial role during ascospore germination of the Pls1 tetraspanin in Podospora anserina provides an example of the convergent evolution of morphogenetic processes in fungal plant pathogens and saprobes.
Eukaryot Cell. 7(10):1809-18

- Espagne E, Lespinet O, Malagnac F, Da Silva C, Jaillon O, Porcel BM, Couloux A, Aury JM, Ségurens B, Poulain J, Anthouard V, Grossetete S, Khalili H, Coppin E, Déquard-Chablat M, Picard M, Contamine V, Arnaise S, Bourdais A, Berteaux-Lecellier V, Gautheret D, de Vries RP, Battaglia E, Coutinho PM, Danchin EG, Henrissat B, Khoury RE, Sainsard-Chanet A, Boivin A, Pinan-Lucarré B, Sellem CH, Debuchy R, Wincker P, Weissenbach J, Silar P. (2008) The genome sequence of the model ascomycete fungus Podospora anserina.
Genome Biol. May 6 ;9(5):R77.

- Malagnac F, Klapholz B, Silar P. (2007)
PaTrx1 and PaTrx3, two cytosolic thioredoxins of the filamentous ascomycete Podospora anserina involved in sexual development and cell degeneration.
Eukaryot Cell. 6(12):2323-31.

- Martins M, Silar P, Dairou J, Malagnac F, Rodrigues-Lima F and Dupret JM. (2007) Cloning and functional characterization of two NAT enzymes in the soil fungus Podospora anserina.
FEBS J. 274:226.

- Malagnac F and Silar P. (2006) Regulation, Cell Differentiation and Protein-Based Inheritance.
Cell Cycle. 5(22):2584-2587

- Haedens V, Malagnac F, Silar P. (2005) Genetic control of an epigenetic cell degeneration syndrome in Podospora anserina.
Fungal Genet Biol. 42(6):564-77.

- Malagnac F, Lalucque H, Lepere G, Silar P. (2004) Two NADPH oxidase isoforms are required for sexual reproduction and ascospore germination in the filamentous fungus Podospora anserina.
Fungal Genet Biol. 41(11):982-97.

- Malagnac F and Silar P. (2003) Non-Mendelian determinants of morphology in fungi.
Curr Opin Microbiol. 6(6):641-5.

- Malagnac F, Bartee L, Bender J. (2002) An Arabidopsis SET domain protein required for maintenance but not establishment of DNA methylation.
EMBO J. 21(24):6842-52.

- Bartee L, Malagnac F, Bender J. (2001) Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.
Genes Dev. 15(14):1753-8.

- Malagnac F, Gregoire A, Goyon C, Rossignol JL, Faugeron G. (1999) Masc2, a gene from Ascobolus encoding a protein with a DNA-methyltransferase activity in vitro, is dispensable for in vivo methylation.
Mol Microbiol 31(1):331-8.

- Malagnac F, Wendel B, Goyon C, Faugeron G, Zickler D, Rossignol JL, Noyer-Weidner M, Vollmayr P, Trautner TA, Walter J. (1997) A gene essential for de novo methylation and development in Ascobolus reveals a novel type of eukaryotic DNA methyltransferase structure.
Cell 91(2):281-90.

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