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Accueil > Départements > Biologie Cellulaire > Sébastien THOMINE : Approches Intégratives du Transport des Ions


DYNANO : Dynamic in vivo imaging of cellular parameters in plants combining fluorescent nano sensors and microfluidic platforms.

Contract type : Labex Saclay Plant Sciences SPS2020
Period : 01/2017-12/2019
Coordinator : Sébastien THOMINE
Staff : Joni Frederick, Fabien Miart, Laetitia Besse

Systems biology approaches in cell biology require a combination of computational modelling with the measurement of cellular processes with a high spatiotemporal resolution in individual cells. This is greatly facilitated with the recent development of genetically encoded nano sensors, which are potentially applicable to many research themes of the SPS (Saclay Plant Sciences). Two main bottlenecks for the adoption of these technologies by SPS scientists are the lack of efficient experimental systems for the observation of rapid changes in cellular parameters and the absence of data analysis procedures to obtain quantitative information on cellular processes in 3D or 4D. DYNANO is a 42 months project addressing these bottlenecks. The project will be carried out by a multidisciplinary team bringing together more than 15 scientists from the three SPS institutes, the hydrodynamics laboratory of the Ecole Polytechnique (LadHyX) and the Elvesys microfluidics company. DYNANO will develop microfluidic chips for the analysis of response kinetics in parallel in multiple roots and will master developing biosensors technologies. Moreover, DYNANO will develop methodologies for the acquisition and analysis of the data. DYNANO partners will use the technologies for the analysis of cytosolic and apoplastic Ca2+, ROS and pH, anion fluxes and heavy metal concentrations, cytoskeleton dynamics, ubiquitination, autophagosome formation, MAP kinase activity, mechanosensing, lipid domain formation and plant microbe interactions. DYNANO is expected to reinforce links with the private sector through the development of new generations of microfluidic chips that can be patented and commercialized and the possibility to develop platforms for the screening of cellular responses to molecules. Finally, the concerted effort around DYNANO is expected to position SPS as a leader in the field of quantitative live cell imaging in plants, which not only should enhance the attractiveness and competitiveness of the SPS teams but also may lead to new scientific breakthroughs leading to innovation.

DYNANO microponic device for Arabidopsis root imaging
DYNANO microponic device for Arabidopsis root imaging
A : Arabidopsis seedlings growing under perfusion on a microponic chip. B : photograph of a root growing in a microfluidic channel. C : fluorescent imaging of root cells in the microponic chip.
Joni Frederick

par MINION - publié le , mis à jour le