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Accueil > Départements > Microbiologie > Nicolas MIROUZE : Transfert horizontal de gènes chez les bactéries pathogènes de l’homme : anciens et nouveaux organismes modèles

Publications de l’équipe

Liste de publications

2018


  • N. Mirouze, C. Ferret, C. Cornilleau, et R. Carballido-López, « Antibiotic sensitivity reveals that wall teichoic acids mediate DNA binding during competence in Bacillus subtilis », Nature Communications, vol. 9, nᵒ 1, p. 5072, 2018.
    Résumé : Despite decades of investigation of genetic transformation in the model Gram-positive bacterium Bacillus subtilis, the factors responsible for exogenous DNA binding at the surface of competent cells remain to be identified. Here, we report that wall teichoic acids (WTAs), cell wall-anchored anionic glycopolymers associated to numerous critical functions in Gram-positive bacteria, are involved in this initial step of transformation. Using a combination of cell wall-targeting antibiotics and fluorescence microscopy, we show that competence-specific WTAs are produced and specifically localized in the competent cells to mediate DNA binding at the proximity of the transformation apparatus. Furthermore, we propose that TuaH, a putative glycosyl transferase induced during competence, modifies competence-induced WTAs in order to promote (directly or indirectly) DNA binding. On the basis of our results and previous knowledge in the field, we propose a model for DNA binding and transport during genetic transformation in B. subtilis.
    Mots-clés : adsorption, architecture, bacterial-cell, biosynthesis, cell-wall, concanavalin-a, deoxyribonucleic-acid, expression, MICROBIO, THG, transcription factor, transforming dna.
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Sélection de publications avant 2018

- Billaudeau C, Chastanet A, Yao Z, Cornilleau C, Mirouze N, Fromion V, Carballido-López R (2017) Contrasting mechanisms of growth in two model rod-shaped bacteria. Nature Communications 8 : 15370.

- Mirouze N, Ferret C, Yao Z, Chastanet A, Carballido-López R (2015) MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis. PLoS Genet 11 : e1005299.


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