Search






Supervisory authorities

CNRS

Our partners


Home > Research > Genome Biology > Yoshiharu YAMAICHI : Integrity of genome and polarity in bacteria

Yoshiharu YAMAICHI : Group Presentation

Our lab is interested in exploring the factors and mechanisms that generate the subcellular and functional organization of bacterial cells, particularly focusing on development and organization of the cell pole, and organization and faithful inheritance of the genome.

Project :

The formation and organization of subcellular domains is critical for numerous cellular processes, even in relatively simple unicellular organisms such as bacteria. Our lab is interested in exploring the factors and mechanisms that generate the subcellular and functional organization of bacterial cells, particularly focusing on development and organization of the cell pole, and organization and faithful inheritance of the genome.

Specific issues we are addressing are; (A) the organization of the cell poles, which constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation, (B) the organization of the chromosomes, which form condensed structures known as nucleoids, and (C) genetic network to interact cellular processes.

We use Vibrio cholerae, a motile Gram-negative rod with unipolar flagellum as model organism. Unlike many other bacteria, the genome of V. cholerae is divided into two circular chromosomes that have been shown to possess replicon-specific systems for replication initiation and segregation, and V. cholerae cells can grow very rapidly without missegregation of the two chromosomes.

Since V. cholerae is the causative agent of cholera, our study has some implications in pathogenesis: motility and chemotaxis are known to be involved in pathogenesis, and ability to multiply is one of the most important virulence factor.

We are always open to explore the frontier of cell biology of bacteria. Indeed, during the expansion of our research on chromosome dynamics and DNA modification, a DNA methyltransferase (MTase) caught our attention. As we discovered the biological role of this MTase on its lord, a transmittable plasmid which also carries multi-drug resistance gene, we are now also working on horizontal gene transfer. Particularly, we are interested in (D) maintenance (faithful segregation), methylation, and horizontal gene transfer of the plasmid. As this plasmid also encodes antibiotic resistance genes, this project features prevention against dissemination of multi-drug resistance bacteria.

Please do not hesitate to contact us with your ideas and inquire about positions available.

Keywords :

Vibrio cholerae, subcellular organization, chromosome dynamics, cell polarity, chemotaxis and motility, horizontal gene transfer (HGT) , maintenance, partitioning and segregation of plasmid, parABS system, adenine methyl transferase (MTase) and DNA methylation, extended-spectrum beta-lactamase (ESBL) and multi-drug resistance

Contact :


YAMAICHI Yoshiharu [Researcher - CNRS]
Integrity of genome and polarity in bacteria [Leader]
01 69 82 3214 Gif - Bât 26

published on , updated on