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Accueil > Départements > Biologie Cellulaire > Anciennes équipes du département > Christien MERRIFIELD : Dynamique du Cytosquelette et Traffic Membranaire



  • B. Antonny, C. Burd, P. De Camilli, E. Chen, O. Daumke, K. Faelber, M. Ford, V. A. Frolov, A. Frost, J. E. Hinshaw, T. Kirchhausen, M. M. Kozlov, M. Lenz, H. H. Low, H. McMahon, C. Merrifield, T. D. Pollard, P. J. Robinson, A. Roux, et S. Schmid, « Membrane fission by dynamin: what we know and what we need to know », The EMBO journal, vol. 35, nᵒ 21, p. 2270-2284, nov. 2016.
    Résumé : The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.
    Mots-clés : Animals, BIOCELL, Cell Membrane, dynamin, Dynamins, ENDEXO, endocytosis, GTPase, Guanosine Triphosphate, Humans, membrane fission, molecular motor.

  • M. Lampe, S. Vassilopoulos, et C. Merrifield, « Clathrin coated pits, plaques and adhesion », Journal of Structural Biology, vol. 196, nᵒ 1, p. 48-56, oct. 2016.
    Résumé : Clathrin mediated endocytosis (CME) is the main route of receptor internalization in mammalian cells and this well conserved mechanism has been intensively studied for over 40yrs. In the general or 'canonical' model of CME clathrin coated pits form stochastically at the plasma membrane and coated pit curvature develops as the coated pit grows through clathrin polymerization. However, the canonical model of CME does not explain the diversity of endocytically active clathrin coated structures (CCSs) found at the plasma membrane by both electron and light microscopy. In this review we examine the canonical model of CME, highlight discrepancies with published experimental data and suggest future avenues of exploration while paying particular attention to the relationship between clathrin coated pits, plaques, sites of adhesion and the formation of endocytic 'hotspots'.
    Mots-clés : BIOCELL, ENDEXO.

  • C. J. Merrifield, « Actin puts the squeeze on Drosophila glue secretion », Nature Cell Biology, vol. 18, nᵒ 2, p. 142-144, janv. 2016.
    Mots-clés : Actomyosin, Animals, BIOCELL, Carrier Proteins, Drosophila melanogaster, Drosophila Proteins, ENDEXO, Exocytosis, Glue Proteins, Drosophila, Membrane Proteins, Salivary Glands, Secretory Vesicles.

  • C. J. Merrifield, « Erratum: Actin puts the squeeze on Drosophila glue secretion », Nature Cell Biology, vol. 18, nᵒ 3, p. 347-347, févr. 2016.


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Lampe M, Pierre F, Al-Sabah S, Krasel C, Merrifield CJ : Dual single scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered b2-adrenergic receptor (2AR) or Mu-opioid receptor (MOR) endocytosis. Mol Biol Cell. 2014 Oct 1 ;25(19):3070-80.

Shevchuk AI, Novak P, Taylor M, Diakonov IA, Ziyadeh-Isleem A, Bitoun M, Guicheney P, Lab MJ, Gorelik J, Merrifield CJ, Klenerman D, Korchev YE. : An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy : J. Cell. Biol. 2012 May 14:197(4):499-508.

Taylor MJ, Lampe M, Merrifield CJ : A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis. PLoS Biol. 2012 April ;e1001302.

*Taylor MJ, Perrais D, Merrifield CJ : A high precision survey of the molecular dynamics of clathrin mediated endocytosis. PLoS Biol. 2011 March ;9(3):e10000604.

*Merrifield CJ, Perrais D, Zenisek D : Coupling between clathrin-coated-pit invagination, cortactin recruitment and membrane scission observed in live cells. Cell. 2005 May 20 ;121(4):593-606.

*Merrifield CJ, Qualmann B, Kessels MM, Almers W : Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts. Eur. J. Cell. Biol. 2004 Feb ;83(1):13-18.

*Merrifield CJ, Feldman ME, Wan L, Almers W : Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. Nat Cell Biol. 2002 Sep ;4(9):691-8.

Merrifield CJ, Rescher U, Almers W, Proust J, Gerke V, Sechi AS, Moss SE : Annexin 2 has an essential role in actin-based macropinocytic rocketing. Curr. Biol. 2001 July ;11:1136-1141.

*Merrifield CJ, Moss SE, Ballestrem C, Imhof BA, Giese G, Wunderlich I, Almers W : Endocytic vesicles move at the tips of actin tails in cultured mast cells. Nat Cell Biol. 1999 May ;1(1):72-4.

* indicates paper with over 100 citations.


Merrifield CJ, Kaksonen M : Endocytic accessory factors and regulation of CME : Endocytosis. Cold Spring Harb Perspect Biol. 2014 March : doi:10.1101.

Merrifield CJ : Fishing for clathrin-coated pit nucleators. Nat Cell Biol. 2012 May 2 ;14(5):452-4.

Merrifield CJ : Seeing is believing : imaging actin dynamics at single sites of endocytosis. Trends Cell Biol. 2004 Jul ; 14(7):352-358.

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