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Accueil > Départements > Biologie Cellulaire > Anne-Marie PRET : Signalisation Cellulaire et Morphogénèse

Publications de l’équipe


  • A. Y. Torres, M. Malartre, A. - M. Pret, et F. Agnès, « JAK/STAT signaling is necessary for cell monosis prior to epithelial cell apoptotic extrusion », Cell Death & Disease, vol. 8, nᵒ 5, p. e2814, mai 2017.
    Résumé : Epithelial cell extrusion is crucial for proper development and tissue homeostasis. High-resolution 3D reconstruction and 4D imaging, combined with genetic analyis, have allowed us to reveal the highly-sterotyped morphogenetic events controlled by JAK/STAT signaling in a developmentally-programmed case of epithelial cell extrusion. Specialized somatic cells, Polar Cells (PCs), are produced in excess and then undergo apoptotic elimination from the follicular epithelium in the Drosophila ovary. We show that supernumerary PCs are first systematically enveloped by PC neighbors on all sides, first laterally, then apically in conjunction with highly-reinforced adherens junctions, and finally basally. The PC to be removed thus loses all contact with follicle cells, germline cells and the basement membrane in a process we have called cell 'monosis', for 'isolation' in Greek. PC monosis takes several hours, and always precedes, and is independent of, activation of apoptosis. JAK/STAT signaling is necessary within the surrounding follicular epithelium for PC monosis. Minutes after monosis is complete, PC apoptotic corpses are formed and extruded laterally within the epithelium, in contrast to the apical and basal extrusions described to date. These apoptotic corpses are engulfed and eliminated by surrounding follicle cells, which are thus acting as non-professional phagocytes. This study therefore shows the non cell-autonomous impact of an epithelium, via JAK/STAT signaling activation, on cell morphogenesis events leading to apoptotic extrusion. It is likely that the use of high-resolution 3D and 4D imaging, which allows for better spatio-temporal understanding of morphogenetic events, will reveal that cell monosis and lateral extrusion within an epithelium are pertinent for other cases of epithelial cell extrusion as well.
    Mots-clés : BIOCELL, SIGDEV.



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Publications principales avant 2015

1. Borensztejn A, Boissoneau E, Fernandez G, Agnès, F, Pret A-M (2013) JAK/STAT autocontrol of ligand-producing cell number through apoptosis. Development 140 : 195-204

2. Bartoletti M, Rubin T, Chalvet F, Netter S, Dos Santos N, Poisot E, Paces-Fessy M, Cumenal D, Peronnet F, Pret A-M, Théodore L. (2012) Genetic Basis for Developmental Homeostasis of Germline Stem Cell Niche Number : A Network of Tramtrack-Group Nuclear BTB Factors. PLoS One 7:e49958

3. Marchal C, Vinatier G, Sanial M, Plessis A, Pret AM, Limbourg-Bouchon B, Théodore L, and Netter S (2012) The HIV-1 Vpu protein induces apoptosis in Drosophila via activation of JNK signaling. PLoS One 7:e34310

4. Khammari, A, Agnès, F, Gandille, P and Pret, A-M (2011) Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1. Cell Death Diff 18 : 793-805

5. Chalvet F, Bartoletti M, Théodore L (2011) Ovary phenotype and expression of bab1 and bab2 paralogs in the ovary of two mutants of the bab locus in Drosophila melanogaster. Drosophila info service 94 : 158-162

6. Gandille, P, Narbonne-Reveau, K. Boissonneau, E, Busson, D and Pret, A-M (2010) The Polycomb Group gene, polyhomeotic, functions in the maintenance of epithelial integrity in Drosophila melanogaster. PLoS One 11:e13946

7. Tricoire, H, Trannoy, S, Lasbleiz, C, Pret, A-M., Monnier, V (2009) The steroid hormone receptor EcR finely modulates Drosophila lifespan during adulthood in a sex specific manner. Mech Ageing Dev 130:547-552

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