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Département Microbiologie

Séminaires Microbiologie

Les événements Microbiologie sont affichés en bleu dans l’agenda I2BC...

La prochaine journée du département aura lieu le 28 avril 2017.
Contact : Nathalie Dautin

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  • Mardi 5 juin 11:30-13:00 - Yoshiaki Kinosita - Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan

    Biophysical approaches to grasp the motility mechanism of microorganisms

    Résumé : The motility of organisms is driven by nano-sized molecular machines that convert chemical energy into mechanical work. The ‘conventional’ motile systems of myosin, kinesin and bacterial flagella have been studied extensively over the last several decades using various approaches such as crystal structure, gene manipulation, and functional analysis. In contrast, I have focused only on the unconventional motile system such as walking of Mycoplasma (1) and swimming archaea (2) using a single-molecular approach for 7 years. In my talk, I will show you the application of microscopic techniques to the molecular motors.
    Additionally, I will also introduce the unforeseen swimming motility of a symbiotic bacterium, Burkholderia sp. RPE64 (3). A bean bug symbiont, Burkholderia sp. RPE64, selectively colonizes the gut crypts by flagella-mediated motility : however, the mechanism for this colonization remains unclear. Remarkably, staining of the flagellar filaments with fluorescent dye Cy3 revealed that the flagellar filaments wrap around the cell body with a motion like that of a ribbon streamer in rhythmic gymnastics. Furthermore, the wrapping motion was also observed in a symbiotic bacterium of the Bobtail squid, Aliivibrio fischeri, suggesting that this motility mode may contribute to migration on the mucus-filled narrow passage connecting to the symbiotic organ.
    (1) Kinosita Y, Nakane D, Sugawa M, Masaike T, Mizutani K, Miyata M, Nishizaka T.
    Unitary step of gliding machinery in Mycoplasma mobile. Proc. Natl. Acad. Sci. USA 111, 8601-8606 (2014)
    (2) Kinosita Y., Uchida, N., Nakane, D. & Nishizaka, T. Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum. Nature Microbiology 1, 16148 (2016).
    (3) Kinosita Y, Kikuchi Y, Mikami N, Nakane D & Nishizaka T. Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body. ISME J 12,838-848 (2017)
    Contact : (Peter Mergaert)

    Lieu : Salle Kalogeropoulos, Bât 400, Université Paris sud - Campus Orsay

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