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Accueil > Départements > Biologie Cellulaire > Sébastien THOMINE : Approches Intégratives du Transport des Ions

Publications de l’équipe

Publications des membres de l’équipe depuis 2008 :


  • E. L. Bastow, V. S. Garcia de la Torre, A. E. Maclean, R. T. Green, S. Merlot, S. Thomine, et J. Balk, « Vacuolar iron stores gated by NRAMP3 and NRAMP4 are the primary source of iron in germinating seeds », Plant Physiology, mai 2018.
    Résumé : During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double nramp3 nramp4 mutant is unable to mobilize Fe stores and does not develop in the absence of external Fe. We used RNA sequencing to compare gene expression in nramp3 nramp4 and wild type during germination and early seedling development. Even though sufficient Fe was supplied, the Fe-responsive transcription factors bHLH38, 39, 100 and 101 and their downstream targets FRO2 and IRT1 mediating Fe uptake were strongly upregulated in the nramp3 nramp4 mutant. Activation of the Fe deficiency response was confirmed by increased ferric chelate reductase activity in the mutant. At early stages, genes important for chloroplast redox control (FSD1, SAPX), Fe homeostasis (FER1, SUFB) and chlorophyll metabolism (HEMA1, NYC1) were downregulated, indicating limited Fe availability in plastids. In contrast, expression of FRO3, encoding a ferric reductase involved in Fe import into the mitochondria, was maintained and Fe-dependent enzymes in the mitochondria were unaffected in nramp3 nramp4. Together these data show that a failure to mobilize Fe stores during germination triggered Fe deficiency responses and strongly affected plastids but not mitochondria.
    Mots-clés : BIOCELL, MINION.

  • Y. Guerringue, S. Thomine, et J. - M. Frachisse, « Sensing and transducing forces in plants with MSL10 and DEK1 mechanosensors », FEBS letters, vol. 592, nᵒ 12, p. 1968-1979, mai 2018.
    Résumé : Mechanosensitive (MS) channels behave as microprobes that transduce mechanical tension into electric and ion signals. The plasma membrane anion-permeable channel AtMSL10 belongs to the first family of MS channels (MscS-LIKE) that has been characterized in Arabidopsis thaliana. In the same membrane, a rapidly activated calcium MS channel activity (RMA) associated with the presence of the DEFECTIVE KERNEL1 (AtDEK1) protein has been recently described. In this Review, based on the comparison of the specific properties of AtMSL10 and RMA, we put forward hypotheses on the mechanism of activation of these two channels, their respective roles in signalling and also raise the question of the molecular identity of RMA. Finally, we propose functions for these two channels within the context of plant mechanotransduction.
    Mots-clés : BIOCELL, mechanosensitive channel, mechanotransduction, MINION, plant.

  • S. Merlot, V. Sanchez Garcia de la Torre, et M. Hanikenne, « Physiology and Molecular Biology of Trace Element Hyperaccumulation », in Agromining: Farming for Metals, A. Van der Ent, G. Echevarria, A. J. M. Baker, et J. L. Morel, Éd. Cham: Springer International Publishing, 2018, p. 93-116.


  • A. Agorio, J. Giraudat, M. W. Bianchi, J. Marion, C. Espagne, L. Castaings, F. Lelièvre, C. Curie, S. Thomine, et S. Merlot, « Phosphatidylinositol 3-phosphate–binding protein AtPH1 controls the localization of the metal transporter NRAMP1 in Arabidopsis », Proceedings of the National Academy of Sciences, p. 201702975, avr. 2017.
    Mots-clés : BIOCELL, DYNBSJ, late endosome, metal transport, MINION, NRAMP, phosphatidylinositol 3-phosphate, vacuole.

  • S. C. Brown, M. Bourge, N. Maunoury, M. Wong, M. W. Bianchi, S. Lepers-Andrzejewski, P. Besse, S. Siljak-Yakovlev, M. Dron, et B. Satiat-Jeunemaître, « DNA remodelling by Strict Partial Endoreplication in orchids, an original process in the plant kingdom », Genome Biology and Evolution, avr. 2017.
    Résumé : DNA remodelling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analysed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodelling observed in 36 out of 41 orchids studied corresponds to strict partial endoreplication. Such process is developmentally regulated in each wild species studied. Cytometry data analyses allowed us to propose a model where nuclear states 2C, 4E, 8E, etc. form a series comprising a fixed proportion, the euploid genome 2C, plus 2 to 32 additional copies of a complementary part of the genome. The fixed proportion ranged from 89% of the genome in Vanilla mexicana down to 19% in V. pompona, the lowest value for all 148 orchids reported. Insterspecific hybridisation did not suppress this phenomenon. Interestingly, this process was not observed in mass-produced epiphytes. Nucleolar volumes grow with the number of endocopies present, coherent with high transcription activity in endoreplicated nuclei. Our analyses suggest species-specific chromatin rearrangement. Towards understanding endoreplication, V. planifolia constitutes a tractable system for isolating the genomic sequences that confer an advantage via endoreplication from those that apparently suffice at diploid level.
    Mots-clés : BIOCELL, CYTO, cytogenetics, cytometry, DYNBSJ, endoreplication, genome imbalance, Genome Size, IMAGIF, MINION, PF, PHOT, Vanilla.

  • E. Diatloff, E. S. Mace, D. R. Jordan, S. Filleur, S. Tai, S. Schmidt, et I. D. Godwin, « The vegetative nitrogen response of sorghum lines containing different alleles for nitrate reductase and glutamate synthase », Molecular Breeding, vol. 37, nᵒ 11, 2017.

  • C. Eisenach, U. Baetz, N. V. Huck, J. Zhang, A. De Angeli, G. J. M. Beckers, et E. Martinoia, « ABA-Induced Stomatal Closure Involves ALMT4, a Phosphorylation-Dependent Vacuolar Anion Channel of Arabidopsis », The Plant Cell, vol. 29, nᵒ 10, p. 2552-2569, 2017.

  • C. Eisenach et A. De Angeli, « Ion Transport at the Vacuole During Stomatal Movements », Plant Physiology, p. pp.00130.2017, avr. 2017.

  • D. Tran, R. Galletti, E. D. Neumann, A. Dubois, R. Sharif-Naeini, A. Geitmann, J. - M. Frachisse, O. Hamant, et G. C. Ingram, « A mechanosensitive Ca(2+) channel activity is dependent on the developmental regulator DEK1 », Nature Communications, vol. 8, nᵒ 1, p. 1009, oct. 2017.
    Résumé : Responses of cells to mechanical stress are thought to be critical in coordinating growth and development. Consistent with this idea, mechanically activated channels play important roles in animal development. For example, the PIEZO1 channel controls cell division and epithelial-layer integrity and is necessary for vascular development in mammals. In plants, the actual contribution of mechanoperception to development remains questionable because very few putative mechanosensors have been identified and the phenotypes of the corresponding mutants are rather mild. Here, we show that the Arabidopsis Defective Kernel 1 (DEK1) protein, which is essential for development beyond early embryogenesis, is associated with a mechanically activated Ca(2+) current in planta, suggesting that perception of mechanical stress plays a critical role in plant development.
    Mots-clés : BIOCELL, MINION.



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-  Pottier, M., Masclaux-Daubresse, C., Yoshimoto, K., & Thomine, S. (2014). Autophagy as a possible mechanism for micronutrient remobilization from leaves to seeds. Front Plant Sci, 5, 11. doi:10.3389/fpls.2014.00011

- Le Bars, R., Marion, J., Le Borgne, R., Satiat-Jeunemaitre, B., & Bianchi, M. W. (2014). ATG5 defines a phagophore domain connected to the endoplasmic reticulum during autophagosome formation in plants. Nat Commun, 5, 4121. doi:10.1038/ncomms5121

- Le Bars, R., Marion, J., Satiat-Jeunemaitre, B., & Bianchi, M. W. (2014). Folding into an autophagosome : ATG5 sheds light on how plants do it. Autophagy, 10(10), 1861-1863. doi:10.4161/auto.29962

-  Aznar, A., Chen, N. W., Rigault, M., Riache, N., Joseph, D., Desmaele, D., Mouille, G., Boutet, S., Soubigou-Taconnat, L., Renou, J. P., Thomine, S., Expert, D., & Dellagi, A. (2014). Scavenging iron : a novel mechanism of plant immunity activation by microbial siderophores. Plant Physiol, 164(4), 2167-2183. doi:10.1104/pp.113.233585

-  Zhang, J., Martinoia, E., & De Angeli, A. (2014). Cytosolic nucleotides block and regulate the Arabidopsis vacuolar anion channel AtALMT9. J Biol Chem, 289(37), 25581-25589. doi:10.1074/jbc.M114.576108

-  Michaud, M., Ubrig, E., Filleur, S., Erhardt, M., Ephritikhine, G., Marechal-Drouard, L., & Duchene, A. M. (2014). Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology. Proc Natl Acad Sci U S A, 111(24), 8991-8996. doi:10.1073/pnas.1402588111

-  Migocka, M., Papierniak, A., Maciaszczyk-Dziubinska, E., Pozdzik, P., Posyniak, E., Garbiec, A., & Filleur, S. (2014). Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana. J Exp Bot, 65(18), 5367-5384. doi:10.1093/jxb/eru295

-  Lanquar, V., Grossmann, G., Vinkenborg, J. L., Merkx, M., Thomine, S., & Frommer, W. B. (2014). Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology. New Phytol, 202(1), 198-208. doi:10.1111/nph.12652

-  Durand-Smet, P., Chastrette, N., Guiroy, A., Richert, A., Berne-Dedieu, A., Szecsi, J., Boudaoud, A., Frachisse, J. M., Bendahmane, M., Hamant, O., & Asnacios, A. (2014). A comparative mechanical analysis of plant and animal cells reveals convergence across kingdoms. Biophys J, 107(10), 2237-2244. doi:10.1016/j.bpj.2014.10.023

-  Peyronnet, R., D. Tran, T. Girault & J.M. Frachisse (2014). Mechanosensitive channels : feeling tension in a world under pressure. Front. Plant Sci. doi : 10.3389/fpls.2014.00558.

-  Wege, S., A. De Angeli, M.J. Droillard, L. Kroniewicz, S. Merlot, D. Cornu, F. Gambale, E. Martinoia, H. Barbier-Brygoo, S. Thomine, N. Leonhardt, S. Filleur (2014). Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid. Sci Signal. 7(333):ra65. doi : 10.1126/scisignal.2005140.

-  Merlot, S., L. Hannibal, S. Martins, L. Martinelli, H. Amir, M. Lebrun, S. Thomine (2014). The metal transporter PgIREG1 from the hyperaccumulator Psychotria gabriellae is a candidate gene for nickel tolerance and accumulation. J. Exp. Bot. 65(6):1551-1564. doi : 10.1093/jxb/eru025.


-  Jaffré, T., Y. Pillon, S. Thomine, S. Merlot (2013).The metal hyperaccumulators from New Caledonia can broaden our understanding of nickel accumulation in plants. Front Plant Sci. 4:279. doi : 10.3389/fpls.2013.00279.

-  Thomine, S., G. Vert (2013). Iron transport in plants : better be safe than sorry. Current Opinion in Plant Biology 16(3):322-7. doi : 10.1016/j.pbi.2013.01.003.

-  De Angeli, A., Baetz, U., Francisco, R., Zhang, J., Chaves, M. M., & Regalado, A. (2013). The vacuolar channel VvALMT9 mediates malate and tartrate accumulation in berries of Vitis vinifera. Planta, 238(2), 283-291. doi:10.1007/s00425-013-1888-y

-  De Angeli, A., Zhang, J., Meyer, S., & Martinoia, E. (2013). AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis. Nat Commun, 4, 1804. doi:10.1038/ncomms2815

-  Zhang, J., Baetz, U., Krugel, U., Martinoia, E., & De Angeli, A. (2013). Identification of a probable pore-forming domain in the multimeric vacuolar anion channel AtALMT9. Plant Physiol, 163(2), 830-843. doi:10.1104/pp.113.219832


-  Clemens, S., M.M. Aarts, S. Thomine, N. Verbruggen (2012). Plant science : the key to preventing slow cadmium poisoning. Trends in Plant Science 18(2):92-9.

-  Gan, Y., Bernreiter, A., Filleur, S., Abram, B., & Forde, B. G. (2012). Overexpressing the ANR1 MADS-box gene in transgenic plants provides new insights into its role in the nitrate regulation of root development.
Plant Cell Physiol, 53(6), 1003-1016. doi:10.1093/pcp/pcs050

-  Robert, N., d’Erfurth, I., Marmagne, A., Erhardt, M., Allot, M., Boivin, K., Gissot, L., Monachello, D., Michaud, M., Duchene, A. M., Barbier-Brygoo, H., Marechal-Drouard, L., Ephritikhine, G., & Filleur, S. (2012). Voltage-dependent-anion-channels (VDACs) in Arabidopsis have a dual localization in the cell but show a distinct role in mitochondria. Plant Mol Biol, 78(4-5), 431-446. doi:10.1007/s11103-012-9874-5


-  Kollist, H., M. Jossier, K. Laanemets, S. Thomine (2011). Anion channels in plant cells. FEBS J. 278(22):4277-4292.

-  Bolte, S., V. Lanquar, M-N. Soler, A. Beebo, B. Satiat-Jeunemaître, K. Bouhidel, S. Thomine (2011). Distinct Lytic Vacuolar Compartments are Embedded Inside the Protein Storage Vacuole of Dry and Germinating Arabidopsis thaliana Seeds. Plant Cell Physiol. 52(7):1142-52.

-  Barbier-Brygoo, H., A. De Angeli, S. Filleur, J-M. Frachisse, F. Gambale, S. Thomine, S. Wege (2011). Anion channels/transporters in plants : from molecular bases to regulatory networks]. Annu Rev Plant Biol. 62:25-51.


-  Diatloff, E., R. Peyronnet, J. Colcombet, S. Thomine, H. Barbier-Brygoo, J-M. Frachisse (2010). R type anion channel : a multifunctional channel seeking its molecular identity]. Plant Signal. Behav. 5:22-27.

-  Thomine, S., H. Barbier-Brygoo (2010). Structural biology : A peep through anion channels] (Comment) Nature 467(7319):1058-9.

-  Wege, S., M. Jossier, S. Filleur, S. Thomine, H. Barbier-Brygoo, F. Gambale, A. De Angeli (2010). The proline 160 in the selectivity filter of the Arabidopsis NO3-/H+ exchanger AtCLCa is essential for nitrate accumulation in planta. Plant J. 63(5):861-9.

-  Migeon, A., D. Blaudez, O. Wilkins, B. Montanini, M.M. Campbell, P. Richaud, S. Thomine, M. Chalot M. (2010). Genome-wide analysis of plant metal transporters, with an emphasis on poplar. Cell. Mol. Life Sci.67(22):3763-84.


-  De Angeli, A., O. Moran, S. Wege, S. Filleur, G. Ephritikhine, S. Thomine, H. Barbier-Brygoo & F. Gambale (2009). ATP binding to the C terminus of the Arabidopsi thaliana nitrate/proton antiporter, AtCLCa, regulates nitrate transport into plant vacuoles. J Biol Chem. 284(39):26526-32.

-  De Angeli, A., D. Monachello, G. Ephritikhine, J-M. Frachisse., S. Thomine., F. Gambale & H. Barbier-Brygoo(2009). Review. . Philos Trans R Soc Lond B Biol Sci 364(1514):195-201.


-  Peyronnet, R., E.S. Haswell, H. Barbier-Brygoo, J-M. Frachisse (2008). AtMSL9 and AtMSL10 : Sensors of plasma membrane tension in Arabidopsis roots. Plant Signaling & Behavior3(9):726-9.

-  Haswell, E.S., R. Peyronnet, H. Barbier-Brygoo, E.M. Meyerowitz & J-M. Frachisse (2008). Two MscS homologs provide mechanosensitive channel activities in the Arabidopsis root. Curr. Biol. 18(10):730-4.

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