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Accueil > Départements > Virologie > Ancienne équipe du département > Jean LEPAULT : Virologie structurale corrélative par cryo-microscopie électronique et radiocristallographie

Publications de l’équipe


  • E. Baquero, A. A. Albertini, H. Raux, A. Abou‐Hamdan, E. Boeri‐Erba, M. Ouldali, L. Buonocore, J. K. Rose, J. Lepault, S. Bressanelli, et Y. Gaudin, « Structural intermediates in the fusion‐associated transition of vesiculovirus glycoprotein », The EMBO Journal, vol. 36, nᵒ 5, p. 679-692, mars 2017.
    Mots-clés : B3S, conformational change, CRYOEM, glycoprotein, IMAPP, intermediate structures, membrane fusion, PF, RHABDO, Vesiculovirus, VIRO, VIROEM.

  • M. Ouldali, V. Maury, G. Nicolas, et J. Lepault, « Photosome membranes merge and organize tending towards rhombohedral symmetry when light is emitted », Journal of Structural Biology, déc. 2017.
    Résumé : Polynoid worm elytra emit light when mechanically or electrically stimulated. Specialized cells, the photocytes, contain light emitting machineries, the photosomes. Successive stimulations induce light intensity variations and show a coupling within and between photosomes. Here, we describe, using electron tomography of cryo-substituted elytra and freeze-fracturing, the structural transition associated to light emission: undulating tubules come closer, organize and their number forming photosomes increases. Two repeating undulating tubules in opposite phase compose the photosome. Undulations are located on three hexagonal layers that regularly repeat and are equally displaced, in x y and z. The tubule membranes within layers merge giving rise to rings that tend to obey to quasi-rhombohedral symmetry. Merging may result either from close-association, hemifusion (one leaflet fusion) or from fusion (two leaflets fusion). Although the resolution of tomograms is not sufficient to distinguish these three cases, freeze-fracturing shows that hemifusion is a frequent process that leads to an reversible anastomosed membrane complex favoring communications, appearing as a major coupling factor of photosome light emission.
    Mots-clés : Cell communication, Cryo-substitution, CRYOEM, Electron tomography, Fusion, Hemifusion, Membrane structure, PF, Photosome, VIRO, VIROEM.


  • N. Scrima, J. Lepault, Y. Boulard, D. Pasdeloup, S. Bressanelli, et S. Roche, « Insights into herpesvirus tegument organization from structural analyses of the 970 central residues of HSV-1 UL36 protein », The Journal of Biological Chemistry, vol. 290, nᵒ 14, p. 8820-8833, avr. 2015.
    Résumé : The tegument of all herpesviruses contains a capsid-bound large protein that is essential for multiple viral processes, including capsid transport, decapsidation at the nuclear pore complex, particle assembly, and secondary envelopment, through mechanisms that are still incompletely understood. We report here a structural characterization of the central 970 residues of this protein for herpes simplex virus type 1 (HSV-1 UL36, 3164 residues). This large fragment is essentially a 34-nm-long monomeric fiber. The crystal structure of its C terminus shows an elongated domain-swapped dimer. Modeling and molecular dynamics simulations give a likely molecular organization for the monomeric form and extend our findings to alphaherpesvirinae. Hence, we propose that an essential feature of UL36 is the existence in its central region of a stalk capable of connecting capsid and membrane across the tegument and that the ability to switch between monomeric and dimeric forms may help UL36 fulfill its multiple functions.
    Mots-clés : Amino Acid Sequence, B3S, Cell Line, Transformed, Crystal Structure, Dimerization, herpesvirus, Herpesvirus 1, Human, HSV1, Humans, IMAPP, Molecular Sequence Data, Protein Conformation, Sequence Homology, Amino Acid, Structural Biology, Tegument, ul36, Viral Proteins, VIRO, VIROEM, Virus Assembly, Virus Structure, vp1/2.
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Principales publications entre 2000 et 2014

- Albertini, A., Bressanelli, S., Lepault, J. and Gaudin, Y. (2011) Structure and working of viral fusion machinery. Curr Top Membr, 68, 49-80.

- Albertini, A.A., Merigoux, C., Libersou, S., Madiona, K., Bressanelli, S., Roche, S., Lepault, J., Melki, R., Vachette, P. and Gaudin, Y. (2012) Characterization of monomeric intermediates during VSV glycoprotein structural transition. PLoS Pathog, 8, e1002556.

- Baquero, E., Albertini, A.A., Vachette, P., Lepault, J., Bressanelli, S. and Gaudin, Y. (2013) Intermediate conformations during viral fusion glycoprotein structural transition. Curr Opin Virol, 3, 143-150.

- Bouthier de la Tour, C., Amrani, L., Cossard, R., Neuman, K.C., Serre, M.C. and Duguet, M. (2008) Mutational analysis of the helicase-like domain of Thermotoga maritima reverse gyrase. J Biol Chem, 283, 27395-27402.

- Chevalier, C., Galloux, M., Pous, J., Henry, C., Denis, J., Da Costa, B., Navaza, J., Lepault, J. and Delmas, B. (2005) Structural peptides of a nonenveloped virus are involved in assembly and membrane translocation. J Virol, 79, 12253-12263.

- Cortez, D., Quevillon-Cheruel, S., Gribaldo, S., Desnoues, N., Sezonov, G., Forterre, P. and Serre, M.C. (2010) Evidence for a Xer/dif system for chromosome resolution in archaea. PLoS Genet, 6, e1001166.

- Desselberger, U., Richards, J., Tchertanov, L., Lepault, J., Lever, A., Burrone, O. and Cohen, J. (2013) Further characterisation of rotavirus cores : Ss(+)RNAs can be packaged in vitro but packaging lacks sequence specificity. Virus Res, 178, 252-263.

- Duguet, M., Serre, M.C. and Bouthier de La Tour, C. (2006) A universal type IA topoisomerase fold. J Mol Biol, 359, 805-812.

- Duquerroy, S., Da Costa, B., Henry, C., Vigouroux, A., Libersou, S., Lepault, J., Navaza, J., Delmas, B. and Rey, F.A. (2009) The picobirnavirus crystal structure provides functional insights into virion assembly and cell entry. Embo J, 28, 1655-1665.

- Elaid, S., Libersou, S., Ouldali, M., Morellet, N., Desbat, B., Alves, I.D., Lepault, J. and Bouaziz, S. (2014) A peptide derived from the rotavirus outer capsid protein VP7 permeabilizes artificial membranes. Biochim Biophys Acta, 1838, 2026-2035.

- Ferlin, A., Raux, H., Baquero, E., Lepault, J. and Gaudin, Y. (2014) Characterization of pH-Sensitive Molecular Switches That Trigger the Structural Transition of Vesicular Stomatitis Virus Glycoprotein from the Postfusion State toward the Prefusion State. J Virol, 88, 13396-13409.

- Fort, P., Albertini, A., Van-Hua, A., Berthomieu, A., Roche, S., Delsuc, F., Pasteur, N., Capy, P., Gaudin, Y. and Weill, M. (2013) Fossil rhabdoviral sequences integrated into arthropod genomes : ontogeny, evolution, and potential functionality. Mol Biol Evol, 29, 381-390.

- Forterre, P., Gribaldo, S., Gadelle, D. and Serre, M.C. (2007) Origin and evolution of DNA topoisomerases. Biochimie, 89, 427-446.

- Galloux, M., Libersou, S., Alves, I.D., Marquant, R., Salgado, G.F., Rezaei, H., Lepault, J., Delmas, B., Bouaziz, S. and Morellet, N. (2010) NMR structure of a viral peptide inserted in artificial membranes : a view on the early steps of the birnavirus entry process. J Biol Chem, 285, 19409-19421.

- Galloux, M., Libersou, S., Morellet, N., Bouaziz, S., Da Costa, B., Ouldali, M., Lepault, J. and Delmas, B. (2007) Infectious bursal disease virus, a non-enveloped virus, possesses a capsid-associated peptide that deforms and perforates biological membranes. J Biol Chem, 282, 20774-20784.

- Letzelter, C., Duguet, M. and Serre, M.C. (2004) Mutational analysis of the archaeal tyrosine recombinase SSV1 integrase suggests a mechanism of DNA cleavage in trans. J Biol Chem, 279, 28936-28944.

- Libersou, S., Albertini, A.A., Ouldali, M., Maury, V., Maheu, C., Raux, H., de Haas, F., Roche, S., Gaudin, Y. and Lepault, J. (2010) Distinct structural rearrangements of the VSV glycoprotein drive membrane fusion. J Cell Biol, 191, 199-210.

- Libersou, S., Siebert, X., Ouldali, M., Estrozi, L.F., Navaza, J., Charpilienne, A., Garnier, P., Poncet, D. and Lepault, J. (2008) Geometric mismatches within the concentric layers of rotavirus particles : a potential regulatory switch of viral particle transcription activity. J Virol, 82, 2844-2852.

- Martin, D., Duarte, M., Lepault, J. and Poncet, D. (2010) Sequestration of free tubulin molecules by the viral protein NSP2 induces microtubule depolymerization during rotavirus infection. J Virol, 84, 2522-2532.

- Pous, J., Chevalier, C., Ouldali, M., Navaza, J., Delmas, B. and Lepault, J. (2005) Structure of birnavirus-like particles determined by combined electron cryomicroscopy and X-ray crystallography. J Gen Virol, 86, 2339-2346.

- Roche, S., Albertini, A.A., Lepault, J., Bressanelli, S. and Gaudin, Y. (2008) Structures of vesicular stomatitis virus glycoprotein : membrane fusion revisited. Cell Mol Life Sci, 65, 1716-1728.

- Roche, S., Bressanelli, S., Rey, F.A. and Gaudin, Y. (2006) Crystal structure of the low-pH form of the vesicular stomatitis virus glycoprotein G. Science, 313, 187-191.

- Roche, S., Rey, F.A., Gaudin, Y. and Bressanelli, S. (2007) Structure of the prefusion form of the vesicular stomatitis virus glycoprotein G. Science, 315, 843-848.
Rouquette, C., Serre, M.C. and Lane, D. (2004) Protective role for H-NS protein in IS1 transposition. J Bacteriol, 186, 2091-2098.

- Rozas-Dennis, G.S., Squires, G., Pous, J., Costabel, M.D., Lepault, J., Navaza, J., Rey, F.A. and Guerin, D.M. (2004) Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans. Acta Crystallogr D Biol Crystallogr, 60, 1647-1650.

- Sao-Jose, C., Lhuillier, S., Lurz, R., Melki, R., Lepault, J., Santos, M.A. and Tavares, P. (2006) The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA. J Biol Chem, 281, 11464-11470.

- Serre, M.C. and Duguet, M. (2003) Enzymes that cleave and religate DNA at high temperature : the same story with different actors. Prog Nucleic Acid Res Mol Biol, 74, 37-81.

- Serre, M.C., El Arnaout, T., Brooks, M.A., Durand, D., Lisboa, J., Lazar, N., Raynal, B., van Tilbeurgh, H. and Quevillon-Cheruel, S. (2012) The carboxy-terminal alphaN helix of the archaeal XerA tyrosine recombinase is a molecular switch to control site-specific recombination. PLoS One, 8, e63010.

- Serre, M.C., Letzelter, C., Garel, J.R. and Duguet, M. (2002) Cleavage properties of an archaeal site-specific recombinase, the SSV1 integrase. J Biol Chem, 277, 16758-16767.

- Serre, M.P. (2006) [Health claims for food : a European outlook]. Ann Pharm Fr, 64, 335-343.

- Stiasny, K., Bressanelli, S., Lepault, J., Rey, F.A. and Heinz, F.X. (2004) Characterization of a membrane-associated trimeric low-pH-induced Form of the class II viral fusion protein E from tick-borne encephalitis virus and its crystallization. J Virol, 78, 3178-3183.

- Stiasny, K., Kossl, C., Lepault, J., Rey, F.A. and Heinz, F.X. (2007) Characterization of a structural intermediate of flavivirus membrane fusion. PLoS Pathog, 3, e20.

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