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Accueil > Départements > Biologie Cellulaire > Béatrice SATIAT-JEUNEMAITRE : Dynamique de la Compartimentation cellulaire dans les cellules de plantes supérieures

Publications de l’équipe


  • T. Avin-Wittenberg, F. Baluška, P. V. Bozhkov, P. H. Elander, A. R. Fernie, G. Galili, A. Hassan, D. Hofius, E. Isono, R. Le Bars, C. Masclaux-Daubresse, E. A. Minina, H. Peled-Zehavi, N. S. Coll, L. M. Sandalio, B. Satiat-Jeunemaitre, A. Sirko, P. S. Testillano, et H. Batoko, « Autophagy-related approaches for improving nutrient use efficiency and crop yield protection », Journal of Experimental Botany, vol. 69, nᵒ 6, p. 1335-1353, mars 2018.
    Mots-clés : BIOCELL, CYTO, DYNBSJ, PF, PHOT.

  • T. Avin-Wittenberg, F. Baluška, P. V. Bozhkov, P. H. Elander, A. R. Fernie, G. Galili, A. Hassan, D. Hofius, E. Isono, R. Le Bars, C. Masclaux-Daubresse, E. A. Minina, H. Peled-Zehavi, N. S. Coll, L. M. Sandalio, B. Satiat-Jeunemaitre, A. Sirko, P. S. Testillano, et H. Batoko, « Corrigendum: Autophagy-related approaches for improving nutrient use efficiency and crop yield protection », Journal of Experimental Botany, vol. 69, nᵒ 12, p. 3173, mai 2018.
    Mots-clés : BIOCELL, CYTO, DYNBSJ, PF, PHOT.

  • R. Enrique Gomez, J. Joubès, N. Valentin, H. Batoko, B. Satiat-Jeunemaître, et A. Bernard, « Lipids in membrane dynamics during autophagy in plants », Journal of Experimental Botany, vol. 69, nᵒ 6, p. 1287-1299, mars 2018.
    Résumé : Autophagy is a critical pathway for plant adaptation to stress. Macroautophagy relies on the biogenesis of a specialized membrane named the phagophore that maturates into a double membrane vesicle. Proteins and lipids act synergistically to promote membrane structure and functions, yet research on autophagy has mostly focused on autophagy-related proteins while knowledge of supporting lipids in the formation of autophagic membranes remains scarce. This review expands on studies in plants with examples from other organisms to present and discuss our current understanding of lipids in membrane dynamics associated with the autophagy pathway in plants.
    Mots-clés : Arabidopsis, autophagy, BIOCELL, DYNBSJ, homeostasis, lipids, membrane, Plant.

  • J. Marion, R. Le Bars, L. Besse, H. Batoko, et B. Satiat-Jeunemaitre, « Multiscale and Multimodal Approaches to Study Autophagy in Model Plants », Cells, vol. 7, nᵒ 1, janv. 2018.
    Résumé : Autophagy is a catabolic process used by eukaryotic cells to maintain or restore cellular and organismal homeostasis. A better understanding of autophagy in plant biology could lead to an improvement of the recycling processes of plant cells and thus contribute, for example, towards reducing the negative ecological consequences of nitrogen-based fertilizers in agriculture. It may also help to optimize plant adaptation to adverse biotic and abiotic conditions through appropriate plant breeding or genetic engineering to incorporate useful traits in relation to this catabolic pathway. In this review, we describe useful protocols for studying autophagy in the plant cell, taking into account some specificities of the plant model.
    Mots-clés : Arabidopsis, autophagosome, Autophagy, autophagy assays, BIOCELL, CYTO, DYNBSJ, methods, PHOT, plant cells, Tobacco.


  • A. Agorio, J. Giraudat, M. W. Bianchi, J. Marion, C. Espagne, L. Castaings, F. Lelièvre, C. Curie, S. Thomine, et S. Merlot, « Phosphatidylinositol 3-phosphate–binding protein AtPH1 controls the localization of the metal transporter NRAMP1 in Arabidopsis », Proceedings of the National Academy of Sciences, p. 201702975, avr. 2017.
    Mots-clés : BIOCELL, DYNBSJ, late endosome, metal transport, MINION, NRAMP, phosphatidylinositol 3-phosphate, vacuole.

  • S. C. Brown, M. Bourge, N. Maunoury, M. Wong, M. W. Bianchi, S. Lepers-Andrzejewski, P. Besse, S. Siljak-Yakovlev, M. Dron, et B. Satiat-Jeunemaître, « DNA remodelling by Strict Partial Endoreplication in orchids, an original process in the plant kingdom », Genome Biology and Evolution, avr. 2017.
    Résumé : DNA remodelling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analysed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodelling observed in 36 out of 41 orchids studied corresponds to strict partial endoreplication. Such process is developmentally regulated in each wild species studied. Cytometry data analyses allowed us to propose a model where nuclear states 2C, 4E, 8E, etc. form a series comprising a fixed proportion, the euploid genome 2C, plus 2 to 32 additional copies of a complementary part of the genome. The fixed proportion ranged from 89% of the genome in Vanilla mexicana down to 19% in V. pompona, the lowest value for all 148 orchids reported. Insterspecific hybridisation did not suppress this phenomenon. Interestingly, this process was not observed in mass-produced epiphytes. Nucleolar volumes grow with the number of endocopies present, coherent with high transcription activity in endoreplicated nuclei. Our analyses suggest species-specific chromatin rearrangement. Towards understanding endoreplication, V. planifolia constitutes a tractable system for isolating the genomic sequences that confer an advantage via endoreplication from those that apparently suffice at diploid level.
    Mots-clés : BIOCELL, CYTO, cytogenetics, cytometry, DYNBSJ, endoreplication, genome imbalance, Genome Size, IMAGIF, MINION, PF, PHOT, Vanilla.

  • S. Chardonnet, T. Bessiron, C. I. Ramos, R. Dammak, M. - A. Richard, C. Boursier, C. Cadillac, F. M. Coquelle, S. Bossi, F. Ango, P. Le Maréchal, P. Decottignies, C. Berrier, H. McLean, et H. Daniel, « Native metabotropic glutamate receptor 4 depresses synaptic transmission through an unusual Gαq transduction pathway », Neuropharmacology, avr. 2017.
    Résumé : In cerebellar cortex, mGlu4 receptors located on parallel fibers play an essential role in normal motor function, but the molecular mechanisms involved are not yet completely understood. Using a strategy combining biochemical and electrophysiological approaches in the rodent cerebellum, we demonstrate that presynaptic mGlu4 receptors control synaptic transmission through an atypical activation of Gαq proteins. First, the Gαq subunit, PLC and PKC signaling proteins present in cerebellar extracts are retained on affinity chromatography columns grafted with different sequences of the cytoplasmic domain of mGlu4 receptor. The i2 loop and the C terminal domain were used as baits, two domains that are known to play a pivotal role in coupling selectivity and efficacy. Second, in situ proximity ligation assays show that native mGlu4 receptors and Gαq subunits are in close physical proximity in cerebellar cortical slices. Finally, electrophysiological experiments demonstrate that the molecular mechanisms underlying mGlu4 receptor-mediated inhibition of transmitter release at cerebellar Parallel Fiber (PF) - Molecular Layer Interneuron (MLI) synapses involves the Gαq-PLC signaling pathway. Taken together, our results provide compelling evidence that, in the rodent cerebellar cortex, mGlu4 receptors act by coupling to the Gαq protein and PLC effector system to reduce glutamate synaptic transmission.
    Mots-clés : BIOCELL, Cerebellar cortex, DYNBSJ, G protein, Molecular layer interneurons, Presynaptic metabotropic glutamate receptor 4, Signaling pathway, Synaptic transmission.

  • J. Marion, R. Le Bars, B. Satiat-Jeunemaitre, et C. Boulogne, « Optimizing CLEM protocols for plants cells: GMA embedding and cryosections as alternatives for preservation of GFP fluorescence in Arabidopsis roots », Journal of Structural Biology, 2017.
    Mots-clés : Arabidopsis, BIOCELL, Correlative microscopy, DYNBSJ, GFP, GMA resin, IMAGIF, MET, PF, PHOT, Tokuyasu, Transmission electron microscopy.

  • P. Pétriacq, L. de Bont, L. Genestout, J. Hao, C. Laureau, I. Florez-Sarasa, T. Rzigui, G. Queval, F. Gilard, C. Mauve, F. Guérard, M. Lamothe-Sibold, J. Marion, C. Fresneau, S. Brown, A. Danon, A. Krieger-Liszkay, R. Berthomé, M. Ribas-Carbo, G. Tcherkez, G. Cornic, B. Pineau, B. Gakière, et R. De Paepe, « Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants », Plant Physiology, vol. 173, nᵒ 1, p. 434-455, 2017.


  • S. Martins, E. M. N. Dohmann, A. Cayrel, A. Johnson, W. Fischer, F. Pojer, B. Satiat-Jeunemaître, Y. Jaillais, J. Chory, N. Geldner, et G. Vert, « Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination », Nature Communications, vol. 6, p. 6151, janv. 2015.
    Mots-clés : Arabidopsis, Arabidopsis Proteins, BIOCELL, Brassinosteroids, Cell Membrane, DYNBSJ, Endosomes, Gene Expression Regulation, Plant, Lysine, Microscopy, Fluorescence, Mutation, Phosphorylation, Plants, Genetically Modified, Polyubiquitin, Protein Kinases, Protein Processing, Post-Translational, Protein Transport, Protein-Serine-Threonine Kinases, Proteolysis, Signal Transduction, UBINET, Ubiquitination, Vacuoles.
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Quelques références avant 2015

- Le Bars R., Marion J., Satiat-Jeunemaitre B., Bianchi M. (2014). Folding into an autophagosome : Atg5 sheds light on how plants do it. Autophagy 10, 1-3

- Molino D., Van der Giessen E., Bathelemy J., Gissot L., Marion J., Vernhettes S., Satiat-Jeunemaître B., Galli T., Tareste D., and Faure J.D. (2014). Very long chain sphingolipids regulate membrane dynamics during plant cytokinesis. Biochim Biophys Acta. 2014 Jul 5 ;1841(10):1422-1430

- Bourge M. , Fort C., Satiat-Jeunemaitre B., Brown S. (2014) Tracking Golgi protein dynamics along the cell cycle : a pulse-chase strategy combining click reaction-revealed EdU and photoconvertible fluorescent reporter. New Phytol, in press.

- Le Bars R., Marion J., Leborgne R., Satiat-Jeunemaitre B., Bianchi M. (2014). Staging autophagosome formation on ER cisternae by AGT5/ATG8 interactions. Nature Com. 5, 4121-4131

- Manil-Ségalen M, Lefebvre C, Jenzer C, Trichet M, Boulogne C, Satiat-Jeunemaitre B, Legouis R. (2014) The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39.Dev Cell. 28(1):43-55 Erratum in : Dev Cell. 2014 Jul 14 ;30(1):110.

- Traverso, J. A., Micalella, C., Martinez, A.*, Brown, S., Satiat-Jeunemaitre, B., Meinnel, T. & Giglione, C. (2013). Roles of N-terminal fatty acid acylations in membrane compartment partitioning : Arabidopsis h-TRXs as a case study. The Plant cell 25, 1056-1077, doi:10.1105/tpc.112.106849 (2013).

- Pereira, C., Pereira, S., Satiat-Jeunemaitre, B. & Pissarra, J. (2013)Two motifs in the aspartic proteinase CdA act as vacuolar sorting determinants and may reflect different routes for vacuolar sorting. Plant Journal, Plant J. 2013 Oct ;76(1):87-100.

- Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Satiat-Jeunemaître B, Brown SC, De Paepe R & Danon A.(2013) Importance of cardiolipin synthase for mitochondrial ultrastructure and respiratory function, plant development and stress responses in Arabidopsis. Plant Cell, 25(10):4195-208.

- Boudiere, L., Botte, C. Y., Saidani, N., Lajoie, M., Marion, J., Brehelin, L., Yamaryo-Botte, Y., Satiat-Jeunemaitre, B., Breton, C., Girard-Egrot, A., Bastien, O., Jouhet, J., Falconet, D., Block, M. A. & Marechal, E.(2012) Galvestine-1, a novel chemical probe for the study of the glycerolipid homeostasis system in plant cells. Molecular bioSystems 8, 2023-2035, 2014,

- Carpentier, A., Abreu, S.*, Trichet, M. & Satiat-Jeunemaitre, B. (2012 Microwaves and tea : new tools to process plant tissue for transmission electron microscopy. Journal of microscopy 247, 94-105,

- Djeddi, A., Michelet, X., Culetto, E., Alberti, A., Barois, N. & Legouis, R. (2012) Induction of autophagy in ESCRT mutants is an adaptive response for cell survival in C. elegans. Journal of cell science 125, 685-694.

- Aubert A , Jessica Marion, Claire Boulogne, Mickael Bourge, Yannick Bellec, Béatrice Satiat-Jeunemaitre (2011) Sphingolipids regulate endomembrane differenciation in plant cells : the BY2 case. Plant J. 65:958-71.

- Bolte S, Lanquar V, Soler MN, Beebo A, Satiat-Jeunemaitre B, Bouhidel A, and Thomine S (2011). Distinct lytic vacuolar compartments embedded inside the Protein Storage Vacuole of dry and germinated Arabidopsis thaliana seeds. Plant Cell Physiol. 2011 Jul ;52(7):1142-52.

- Bach, L., Gissot, L., Marion, J., Tellier, F., Moreau, P., Satiat-Jeunemaitre, B., Palauqui, J. C., Napier, J. A. & Faure, J. D. (2011) Very-long-chain fatty acids are required for cell plate formation during cytokinesis in Arabidopsis thaliana. Journal of Cell Science 124, 3223-3234,

- Markham J. , Diana Molino, Lionel Gissot, Yannick Belleck Kian He´maty, Jessica Marion, Katia Belcram,b Jean-Christophe Palauqui, Béatrice Satiat-Jeunemaitre, and Jean-Denis Faure (2011). Sphingolipids Containing Very-Long-Chain Fatty Acids Define a Secretory Pathway for Specific Polar Plasma Membrane Protein Targeting in Arabidopsis. The Plant cell 23, 2362-2378

- Coquelle F.M., Blestel S., Heichette C., Arnal I., Kervrann C. and Chrétien D. « Cryo-Electron Tomography of Microtubules Assembled in vitro from Purified Components ». Methods Mol. Biol. 2011 ; 777 : 193-208.

- Van de Velde W., Zehirov G., Szatmari A., Debreczeny M., Ishihara H., Kevei Z., Farkas A., Mikulass K., Nagy A., Tiricz H., Béatrice Satiat-Jeunemaître, Alunni B., Bourge M., Kucho K., Abe M., Kereszt A. Maroti G., Uchiumi T., Kondorosi E., Mergaert P. (2010). Plant Peptides Govern Terminal Differentiation of Bacteria in Symbiosis. Science, 327 , 1122-1126

- Brown S. Susanne Bolte, Marie Gaudin, Claudia Pereira, Jessica Marion, Marie-Noëlle Soler and Béatrice Satiat-Jeunemaitre (2010) Exploring plant endomembrane dynamics using the photoconvertible protein Kaede . Plant J, 63, pp 696–711

- Frei dit Frey, Muller P, Jammes F, Kizis D, Leung J, Perrot-Rechenmann C, Bianchi MW. Plant Cell. 2010 May ; 22(5):1575-91.
Philippe Laporte, Béatrice Satiat-Jeunemaître, Isabel Velasco, Tibor Csorba, Willem Van de Velde, Anna Campalans, Joszef Burgyan, Miguel Arevalo-Rodriguez and Martin Crespi (2010) A novel RNA-binding peptide regulates the establishment of the Medicago truncatula–Sinorhizobium meliloti nitrogen-fixing symbiosis. Plant J., 62, 24-38

- Coquelle F.M.*, Vitre B. and Arnal I.* « Structural basis of EB1 effects on microtubule dynamics ». *Corresponding authors. Biochem Soc Trans. 2009 Oct ;37(Pt 5):997-1001.

- Dif A., Bouldemais F., Pinot M., Roullier V., Baudy-Floc’h M., Coquelle F.M., Clarke S., Neveu P., Vignaux F., Le Borgne R., Dahan M., Guéroui Z. and Marchi-Artzner V. « Small Peptidic PEGylated Quantum dots to target polyhistidine-tagged proteins with controlled stoichiometry ». J Am Chem Soc. 2009 Oct 21 ;131(41):14738-46.

- Raffaele, S., Bayer, E., Lafarge, D., Cluzet, S., German Retana, S., Boubekeur, T., Leborgne-Castel, N., Carde, J. P., Lherminier, J., Noirot, E., Satiat-Jeunemaitre, B., Laroche-Traineau, J., Moreau, P., Ott, T., Maule, A. J., Reymond, P., Simon-Plas, F., Farmer, E. E., Bessoule, J. J. & Mongrand, S. (2009) Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement. The Plant Cell 21, 1541-1555,

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