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  • Génomes

    • Vendredi 13 mars 14:00-15:00 - Stefan HOFFMANN - University of Manchester, Royaume-Uni

      Continuous Culture in Directed Evolution Studies

      Résumé : Directed evolution is a powerful tool in protein engineering to achieve desired protein characteristics by selection, without requiring structural knowledge. Traditionally, in vivo selections are done in batch culture, which is characterised by a dynamically changing medium composition as cells multiply. This can make challenging selections difficult, especially dual selections for switchable function, due to affecting cell physiology and limited control over the selective stringency. To address this limitation, we have developed a continuous culture bioreactor system with optical feedback control and a volume-independent on-line estimation of growth rates. This small-scale turbidostat ( 20 ml) affords microbial cultures at freely chosen constant densities, enabling selections at steady physiological states with the capability of monitoring population fitness over time. It also facilitates longer selections typical for adaptive laboratory evolution experiments, with the growth rate assessment providing guidance for adjusting selective pressure.

      Contact : Christophe POSSOZ <Christophe.POSSOZ>

      Lieu : Salle des séminaires - bâtiment 26 - campus de Gif-sur-Yvette


  • B3S

    • Jeudi 5 mars 10:30-12:00 - Maya Wright and Stéphanie Bourgeois - Fluidic Analytics Limited, Cambridge, UK

      The Fluidity One-W from Fluidic Analitytic : measurement of binding affinities using microfluidic diffusional sizing

      Résumé : The Fluidity One-W is an equipement allowing the measurement of binding affinities using microfluidic diffusional sizing (MDS).
      To have more information :
      Invited by Paola Llinas

      JPEG - 281.3 ko

      Lieu : Bibliothèque bât.34


  • cytoskeleton club

    • Mardi 10 mars 11:30-12:30 - Nicolas Borghi - Institut Jacques Monod, Paris

      Cytoskeleton club - Force transmission at cell adhesions and the nucleus

      Résumé : In multicellular organisms, cells generate and undergo mechanical forces that may shape cells, tissues and organs, but also regulate genetic programs. The recent advent of genetically-encoded sensors of molecular tension has allowed to provide quantitative, molecular-scale and molecular-specific information on these forces. We have leveraged these tools in proteins of cell adhesion and of the nuclear envelope to address how forces transmit across scales and regulate signaling pathways during model morphogenetic processes in cell culture. Our results reveal non-trivial relationships between molecular-scale and tissue-scale forces, and show how signaling pathways targeting gene transcription involve mechanotransduction events at multiple levels.

      Lieu : Bibliothèque - bât. 34


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