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3 octobre 2017: 1 événement

  • Département Microbiologie

    Mardi 3 octobre 2017 11:30-12:30 - Dr Alexandre Chenal - Groupe BiophysiCyaA Laboratoire de Biochimie des Intéractions Macromoléculaires Département de Biologie Structurale et Chimie Institut Pasteur, Paris

    La toxine CyaA de Bordetella pertussis : de la biogénèse à la piraterie

    Résumé : The adenylate cyclase toxin (CyaA, 1706 residues) is an RTX protein that plays an essential role in the early stages of respiratory tract colonization by Bordetella pertussis, the causative agent of whooping cough. Its cell intoxication process, however, is still poorly understood. After its secretion through a dedicated type 1 secretion system, CyaA intoxicates human cells via a unique mechanism of translocation of its catalytic domain (AC) directly across the plasma membrane of target cells. Once in the cytosol, AC interacts with calmodulin (CaM) and produces supraphysiological levels of cAMP, leading to cell death. I will present some recent data, which covers several steps of this intoxication process. Our results illustrate the structural flexibility of bacterial toxins adapted to various functions and contexts, such as toxin secretion and refolding, AC translocation, and enzymatic AC:CaM complex formation. Our data further shows the adaptation of bacterial RTX toxins to the diverse array of calcium concentrations encountered in the successive environments during the intoxication process. Finally, due to its hydrophobic character, CyaA is known for its propensity to aggregate into multimeric forms in the absence of a chaotropic agent in vitro. We have recently defined the experimental conditions required for CyaA folding into a stable, monomeric and functional form. This opens new perspectives for both basic science and CyaA-based biotechnological applications developed in the lab, i.e., to improve antigen delivery vehicles and new pertussis vaccines.

    Lieu : Salle A. Kalogeropoulos - Bâtiment 400, campus d’Orsay

    En savoir plus : Département Microbiologie

3 octobre 2017: 1 événement

  • Evenements 3D_CHROME

    Du 2 octobre 2017 13:00 au 3 octobre 2017 18:00 -

    3D-CHROME Workshop : “Modelling of 3D genome organisation”

    Résumé : MGO2017 Workshop - “Modelling of 3D Genome Organization - Integrating genomics and microscopy data”
    Chromosomes need to be precisely organized within the limited nuclear volume that is available in interphase. Genomics and microscopy studies have revealed that 3D genome organization directly mirrorsbiological functions like gene regulation, recombination and DNA replication and repair. Biophysical and mathematical modelling can provide further insights whether 3D genome organization dictates functional outputs and how much variation is present between individual cells and over time.
    During the workshop 8 internationally renowned experts will discuss recent projects where genomics and microscopy studies are integrated using biophysical and modelling approaches.
    Confirmed speakers :
    • Kerstin Bystricky - LBME, Toulouse
    • Luca Giorgetti, FMI, Switzerland
    • Ivan Junier, TIMC-IMAG, Grenoble
    • Jop Kind, Hubrecht Institute, The Netherlands
    • Julien Mozziconacci, UPMC, Paris
    • Mario Nicodemi, University of Naples and INFN, Italy
    • Cédric Vaillant, Ecole Normale Supérieure, Lyon
    • Juan Vaquerizas, MPI for Molecular Biomedicine, Germany
    The workshop is aimed at scientists with backgrounds in (bio)physics, biology and bioinformatics (PhD students, postdocs, engineers, young and senior scientists). Registration is free of charge but mandatory ( number of participants is strictly limited to 150 participants, on a first-come basis, with deadline on the 24th of July. We therefore advise to register as soon as possible. The workshop is organized by the University Paris-Saclay 3D-CHROME Initiative with support from LABEX PALM and LABEX SPS.
    The workshop will be followed by a half-day bioinformatics tutorial on Hi-C data analysis (afternoon of October 3rd) where tools for Hi-C data processing and the calling of functional domains will be discussed by Nicolas Servant (Insitut Curie) and Kai Kruse (MPI for Molecular Biomedicine, Germany). Registration to this tutorial is limited to 20 participants, with selection based on motivation. The tutorial is organized by the University Paris-Saclay PSay-CompBioInitiative.
    Further information at and in the flyer.

    Lieu : Auditorium - Bâtiment 21, campus de gif

    En savoir plus : Evenements 3D_CHROME