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15 décembre 2017: 1 événement

  • Département Biologie des Génomes

    Vendredi 15 décembre 2017 11:00-12:00 - Wouter de Laat - Biomedical genomics group, Hubrecht Institute, Utrecht, The Netherlands

    Genome structure-function relationships explored at new dimensions

    Résumé : Chromatin folding is increasingly recognized as a regulator of genomic processes such as gene activity. Chromosome conformation capture (3C) methods have been developed to unravel genome topology through the analysis of pair-wise chromatin contacts and have identified many genes and regulatory sequences that, in populations of cells, are engaged in multiple DNA interactions. I will describe our contributions to this field and will present novel Multi-Contact 4C (MC-4C), that applies Oxford Nanopore long-read single molecule sequencing to study multi-way DNA conformations of individual alleles for distinction between cooperative, random and competing interactions. Using this single molecule multi-contact analysis method, we uncover previously missed structures in sub-populations of cells, reveal single locus enhancer hubs and demonstrate the collision of CTCF-anchored architectural loops. Insight into single allele higher-order topological features will help understanding how the myriad of regulatory sequences spatially coordinate their actions on individual chromosomes and facilitate interpreting the consequences of natural and induced genetic variation. If time permits, I will also discuss how we explore the application of 3C-based methods in DNA diagnostics, oncogenetics and non-invasive prenatal diagnosis.
    Contact : Daan Noordermeer <daan.noordermeer>

    Lieu : Auditorium - bâtiment 21 - Campus CNRS de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes