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12 juin 2018: 1 événement

  • Cytoskeleton club

    Mardi 12 juin 11:30-12:30 - Hong Wang

    Cytoskeleton club

    Résumé : Talin inhibits actin polymerization - Focal adhesions (FAs) play a major role in many physiological functions. Focal adhesions mechanically couple the extracellular matrix (ECM) to the dynamic and contractile actomyosin cytoskeleton, via transmembrane receptors of the integrin family and actin-binding proteins (ABPs). Among these ABPs, talin plays a major role, including activating integrins and anchoring cytoskeleton to FAs. Talin recruits the ABP vinculin in response to force which also associate to the ABP VASP. Although the ability of vinculin and VASP to regulate actin assembly is known, talin activity has never been studied. Our project is to understand the ability of the talin-vicnulin-VASP complex to regulate actin assembly. As preliminary step, we characterized talin activity. Talin contains three actin-binding domains (ABDs). ABD1 spans along the F2 and F3 subdomains of the FERM domain. ABD2 is located in the center of the rod. ABD3, is located at the C-terminus of the protein. Here we use kinetic assay of actin polymerization in fluorescence spectroscopy and actin filament observation in Total Internal Reflection Fluorescence Microscopy (TIRF) to study talin activity. The detail characterization of talin ABD1 demonstrates that it blocks actin filament barbed end elongation.

    Lieu : Bibliothèque - Bâtiment 34, Campus de Gif

    En savoir plus : Cytoskeleton club