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29 juin 2018: 1 événement

  • Département Biologie des Génomes

    Vendredi 29 juin 2018 11:00-12:00 - Edouard Bertrand - Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier

    A dual protein/RNA localization screen reveals specialized translation factories in human cells

    Résumé : Abstract
    Local translation provides a spatial control of gene expression. We performed a dual protein/mRNA localization screen, using smFISH on a collection of 520 HeLa cell lines carrying GFP-tagged genes. We found that 32 RNAs had particular cytoplasmic localizations, with accumulation in P-bodies being to most frequent (17 cases). Eight mRNAs colocalized with their encoded protein, and local translation occurred at various cellular locations, including cytoplasmic extensions, endosomes, and the Golgi apparatus. Interestingly, four mRNAs accumulated in cytoplasmic foci that were not P-bodies and that were distinct from each other. We used the SunTag system to visualize mRNA translation in live cells and at the level of single molecules, and we showed that these four mRNA foci correspond to specialized translation factories. Remarkably, in one case, we found that the factory functions to destruct the nascent protein, and the data further suggest a mechanism for their formation. Extrapolating the results to the entire genome suggests that human cells may contain more than a hundred of such translation factories. They may allow mRNAs to be translated by specialized machineries, including specialized ribosomes.
    Contact : MIRANDE Marc <Marc.MIRANDE>

    Lieu : Bibliothèque- bâtiment 34 - Campus CNRS de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes