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14 septembre 2018: 1 événement

  • Département Biologie des Génomes

    Vendredi 14 septembre 2018 11:00-12:00 - Hironori Niki - National Institute for Genetics (NIG), Japan

    A long-periodical expression of trj1, a key gene responsible for fungal temperature response, in the dimorphic fission yeast Schizosaccharomyces japonicas

    Résumé : Many fungi respond to environmental cues including light and temperature in order to regulate fungal development and behavior. The WC proteins are assembled in a white collar complex (WCC) that is the major transcription factor of the light response in fungi. Orthologs of the WC proteins are found in dimorphic fission yeast Schizosaccharomyces japonicus. Indeed, hyphae of Sz. japonicus show synchronous activation of cell division in response to light. Besides, thermal stimuli can induce the similar response under continuous darkness (Okamoto et al. 2013). When temperature cycles (30°C for 12 h and 35°C for 12 h) were applied, distinct dark- and bright-colored stripes of hyphae were formed on an agar plate. We identified a key gene for the temperature response in our knockout gene library of Sz. japonicus. The gene, trj1, encodes a 63 kDa protein including the BTB/POZ domain, which is involved in protein-protein interaction. Orthologs of trj1 are broadly conserved in the kingdom Fungus alone. We analyzed the expression of tri1 after the temperature stimuli. Interestingly, tri1 showed a long-period change in the gene expression. After temperature shift-up, transcription started to increase mRNA of trj1, reached a maximum level after 18 h, and finally got back after 36 h. The regular expression patterns of trj1 were observed between 25°C and 35°C, suggesting the regular expression is temperature-compensated. Although the gene expression changed for over a day, the regular expression pattern of trj1 oscillated with 24 hours period during the temperature cycles. Both light and temperature, which are daily external cues, have the same effect on growing hyphal cells in Sz. japonicus. The dual sensing mechanism of external signals allows the organism to adapt to daily changes of environmental alteration.
    Contact : Yoshiharu YAMAICHI <Yoshiharu.YAMAICHI>

    Lieu : Salle des séminaires- bâtiment 26 - Campus CNRS de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes