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25 janvier 2019: 1 événement

  • Département Biologie des Génomes

    Vendredi 25 janvier 11:00-12:00 - Nathalie Beaujean - Stem Cell & Brain Institute – Lyon

    Epigenetic reprogramming of rabbit stem cells

    Résumé : Abstract
    Pluripotency is the term used to describe the ability of a stem cell to give rise to all cell types in mature organisms. Two main types of pluripotent stem cells (PSCs) have been described : naïve and primed. The naïve pluripotent state is characteristic of mouse embryonic stem cells (mESCs) and developmentally corresponds to the early epiblast component of the blastocyst stage (just before implantation). Under appropriate conditions, mESCs exhibit unlimited self-renewal capacity while retaining the attributes of preimplantation epiblast identity and potency – especially the ability to generate chimeras.
    On the other hand, in non-rodent species, including humans, it is challenging to capture this original “embryonic” state of pluripotency in PSCs that are mostly in the primed state. In particular, the scarcity of primate embryos makes it difficult to address this issue. As a surrogate model, the rabbit is perfectly suited to explore the nature and mechanisms of acquisition and maintenance of pluripotency in the epiblast cells and ESCs. We therefore explore naïve pluripotency in the rabbit.
    We characterised the transcriptome of the rabbit epiblast throughout pre-implantation development using single-cell analysis. We observed a transcriptomic continuum of pluripotency in vivo specific to the rabbit, although we observed many similarities to the mouse. We also identified new genes and rabbit-specific markers of naïve pluripotency that could be used for reprogramming conventional rabbit PSCs to naïve-state pluripotency ; in particular genes related to the epigenome reprogramming occurring during embryonic development such as the histone acetyltransferase Kat8. We are now implementing new strategies using the identified markers to reprogram rabbit PSCs towards the naïve state. These results will allow us to judge the state of pluripotency of rabbit PSCs we will derive in vitro, as compared to the embryo.
    Contact : Daan Noordermeer <daan.noordermeer i2bc.paris-saclay.fr>

    Lieu : Bibliothèque- bâtiment 34 - Campus CNRS de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes