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15 février 2019: 1 événement

  • Département Biologie des Génomes

    Vendredi 15 février 2019 11:30-12:30 - Martial Marbouty - Institut Pasteur

    Contact genomic : a new tool to study microbial communities and unveil mobile elements - bacteria relationships in mammalian gut microbiota

    Résumé : Metagenome sequence analysis relies principally on compositional approaches, which hypothesise that sequences sharing similar characteristics (GC%, codon bias, coverage-covariation... etc.) should share the same cellular compartment. Although these approaches have generated important results, they remain somehow limited and do not allow the full characterization and understanding of the genetic composition of a complex microbial population. Contact genomics, which aimed at exploiting the 3D physical signature of genomes to solve their sequence, has the potential to alleviate or improve some of these caveats. To explore the genomic content of bacterial populations at a new level of resolution, we have recently developed meta3C (Marbouty et al. 2014), a derivative protocol of the chromosome conformation capture (3C ; Dekker et al. 2002) assay that aims at deciphering the average 3D organization of a genome. Using controlled mixes of bacterial or yeast species, we showed that the frequent collisions experienced by DNA molecules sharing similar cellular compartments can be measured through meta3C and conveniently used to assemble larger scaffolds of the genomes present in a metapopulation.
    Here I will present data obtained from different samples of mammalian gut microbiota (Marbouty et al. 2017). Meta3C allows unveiling hundreds of genomic compartments, hence species. Moreover, Meta3C allows to link mobile genetic element sequences to their bacterial host and provide a convenient way to study interactions between genomic entities in a complex population. I will discuss the different ways to explore this network and the results in light of the promising potential of the approach for future applications.
    Contact : Mireille Bétermier <mireille.betermier>

    Lieu : Salle des séminaires- bâtiment 26 - Campus CNRS de Gif-sur-Yvette

    Notes de dernières minutes : CHANGE OF TIME : now it starts at 11h30 (instead of 11h00)

    En savoir plus : Département Biologie des Génomes