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21 juin 2019: 2 événements

  • Département Biologie des Génomes

    Vendredi 21 juin 11:00-12:00 - Lori A. PILE - Department of Biological Sciences, Wayne State University, Detroit, Michigan, USA

    Determination of gene regulatory and metabolic networks by an essential histone modifying complex

    Résumé : Regulation of the spatial and temporal patterns of gene expression is central to every aspect of organismal development, normal physiological function and is directly linked to numerous diseases. Histone modifying enzymes that add or remove histone marks regulate chromatin structure to promote or repress gene activity. Among the key complexes that regulate the epigenetic signatures on histones is the SIN3 histone modifying complex, which contains both a deacetylase and a demethylase enzyme. How these factors coordinately regulate expression of specific gene sets to control cell proliferation and developmental transitions is largely unknown. Through analysis of chromosomal localization patterns and gene expression profiles, we determined genome-wide functional regulatory activity of the two major isoforms of Drosophila SIN3. Because we found that both isoforms regulate genes encoding key metabolic enzymes, we next identified metabolites controlled by SIN3. These experiments have uncovered SIN3 controlled gene and metabolic networks linking epigenetic regulation by SIN3 to central carbon metabolism necessary for cellular energy production.

    Lieu : Salle des séminaires - bâtiment 26 - campus de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes
  • Département Biologie Cellulaire

    Vendredi 21 juin 11:30-12:30 - Aeid IGBARIA - COSS Laboratory, Université de Rennes

    ER resident proteins reflux, a functional ER surveillance mechanism during stress and disease

    Résumé : Diverse perturbations to endoplasmic reticulum (ER) functions compromise the proper folding and structural maturation of secretory proteins causing ER stress. Recently, we found that diverse ER stress regimes cause properly folded, ER-resident eroGFP (and other ER luminal proteins) to “reflux” back to the reducing environment of the cytosol as intact, folded proteins. By performing a comprehensive genetic screen in Saccharomyces cerevisiae, we found that ER protein reflux during ER stress requires specific chaperones and cochaperones residing in both the ER and the cytosol. Chaperone mediated ER protein reflux proceeds more vigorously when ER-associated degradation (ERAD) factors are crippled, suggesting that reflux may work in parallel with ERAD. Finally, we found that ER protein reflux is conserved in mammalian cells, regulated by the IRE1a kinase/endoribonuclease and may play preeminent role in human diseases. In summary, chaperone-mediated ER protein reflux may play as a conserved protein quality control process that evolved to maintain secretory pathway homeostasis during ER protein-folding stress.

    Lieu : Bibliothèque - bâtiment 34 - campus de Gif-sur-Yvette

    En savoir plus : Département Biologie Cellulaire