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18 octobre 2019: 1 événement

  • Département Biologie des Génomes

    Vendredi 18 octobre 11:00-12:00 - Heath MURRAY - Newcastle University, Royaume Uni

    Identification of a basal system for bacterial chromosome origin unwinding

    Résumé : Genome duplication is essential for cell proliferation and DNA synthesis is generally initiated by dedicated replication proteins at specific loci termed origins. In bacteria the master initiator DnaA binds the chromosome origin (oriC) and unwinds the DNA duplex to permit helicase loading. However, despite decades of research it remained unclear how the information encoded within oriC guides DnaA-dependent strand separation. To address this fundamental question, we took a systematic genetic approach in vivo and identified the core set of essential sequence elements within the Bacillus subtilis chromosome origin unwinding region. Using this information we then show in vitro that the minimal replication origin sequence elements are necessary and sufficient to promote the mechanical functions of DNA duplex unwinding by DnaA. Because the basal DNA unwinding system characterized here appears to be conserved throughout the bacterial domain, this discovery provides a framework for understanding oriC architecture, activity, regulation and diversity.


    Contact : Jean-Luc FERAT (jean-luc.ferat i2bc.paris-saclay.fr)

    Lieu : Salle des séminaires - bâtiment 26 - Campus de Gif-sur-Yvette

    En savoir plus : Département Biologie des Génomes