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26 février 2021: 1 événement

  • Département Biologie des Génomes

    Vendredi 26 février 11:00-12:00 - Sebastian Baumgarten - Pasteur Institute

    Resolving m6A-mediated post-transcriptional control in the human malaria parasite

    Résumé : Post-transcriptional regulation of gene expression is central to the development and replication of the malaria parasite, Plasmodium falciparum, within its human host. The timely coordination of RNA maturation, homeostasis and protein synthesis relies on the recruitment of specific RNA-binding proteins to their cognate target mRNAs. Our work focuses on the role of RNA modifications as one possible mediator of such mRNA-protein interactions. Using mass spectrometry and different RNA sequencing approaches, we identified N6-methylation of adenosines (m6A) as the most highly abundant and developmentally regulated mRNA modification, exceeding m6A levels known in any other eukaryote. Combining targeted genome editing, RNA protein-pulldowns and interaction proteomics, we characterized the m6A methyltransferase ‘writer’, and ‘reader’ complex that effect m6A-medited post-transcriptional control, thereby revealing a role for m6A in mediating transcript stability and translational efficiency. Our recent work has laid the foundation to interrogate this extensive epitranscriptomic program in this unicellular eukaryote, with our current focus laying on elucidating its role in fine-tuning the transcriptional cascade throughout the parasite’s lifecycle.
    Invited by Joana.Santos

    Lieu : visio

    En savoir plus : Département Biologie des Génomes