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Accueil > Départements > Biochimie, Biophysique et Biologie Structurale > Françoise OCHSENBEIN & Raphaël GUEROIS : Assemblage moléculaire et intégrité du génome

Françoise OCHSENBEIN & Raphaël GUEROIS : Assemblage moléculaire et intégrité du génome

Françoise OCHSENBEIN & Raphaël GUEROIS : Présentation de l’équipe

Notre équipe s’appuie sur un couplage fort entre approches bioinformatiques et expérimentales pour élucider les bases moléculaires qui régissent l’assemblage dynamique des protéines. Focalisés sur les (...)

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Les membres

Anciens membres de l’équipe
May BAKAIL, ancienne doctorante (2013-2016), maintenant post-doc à l’institut Cochin
Jinchao YU, ancien doctorant (2013-2016), maintenant chez Pharnext
Nicolas (...)

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Publications de l’équipe

Les 10 dernières publications

Biochimie. 2018 Jun 08;:
Emerging fields in chaperone proteins : a French workshop. Authors: Mileo E, Ilbert M, Barducci A, Bordes P, Castanié-Cornet MP, Garnier C, Genevaux P, Gillet R, Goloubinoff P, Ochsenbein F, Richarme G, Iobbi-Nivol C, Giudici-Orticoni MT, Gontero B, Genest O [PubMed]

FEBS J. 2019 Feb 16;:
The C-terminal domain of HpDprA is a DNA-binding Winged Helix domain that does not bind double-stranded DNA. Authors: Lisboa J, Celma L, Sanchez D, Marquis M, Andreani J, Guérois R, Ochsenbein F, Durand D, Marsin S, Cuniasse P, Radicella JP, Quevillon-Cheruel S [PubMed]

PLoS One. 2017;12(12):e0189049
Structural basis for the substrate selectivity of Helicobacter pylori NucT nuclease activity. Authors: Celma L, Corbinais C, Vercruyssen J, Veaute X, de la Sierra-Gallay IL, Guérois R, Busso D, Mathieu A, Marsin S, Quevillon-Cheruel S, Radicella JP [PubMed]

iScience. 2019 Feb 19;13:138-153
Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. Authors: Omrane M, Camara AS, Taveneau C, Benzoubir N, Tubiana T, Yu J, Guérois R, Samuel D, Goud B, Poüs C, Bressanelli S, Garratt RC, Thiam AR, Gassama-Diagne A [PubMed]

Proc Natl Acad Sci U S A. 2019 Aug 16;:
The TLCΦ satellite phage harbors a Xer recombination activation factor. Authors: Midonet C, Miele S, Paly E, Guerois R, Barre FX [PubMed]

Elife. 2018 Jul 09;7:
Rad52-Rad51 association is essential to protect Rad51 filaments against Srs2, but facultative for filament formation. Authors: Ma E, Dupaigne P, Maloisel L, Guerois R, Le Cam E, Coïc E [PubMed]

J Biol Chem. 2018 Jul 19;:
Characterization of the binding mode of JNK-interacting protein 1 (JIP1) to kinesin-light chain 1 (KLC1). Authors: Nguyen TQ, Aumont-Niçaise M, Andreani J, Velours C, Chenon M, Vilela F, Geneste C, Varela PF, Llinas P, Menetrey J [PubMed]

DNA Repair (Amst). 2019 Mar 22;77:96-108
Identification of a miniature Sae2/Ctp1/CtIP ortholog from Paramecium tetraurelia required for sexual reproduction and DNA double-strand break repair. Authors: Godau J, Ferretti LP, Trenner A, Dubois E, von Aesch C, Marmignon A, Simon L, Kapusta A, Guérois R, Bétermier M, Sartori AA [PubMed]

Virology. 2016 May 30;496:42-50
Phagonaute : A web-based interface for phage synteny browsing and protein function prediction. Authors: Delattre H, Souiai O, Fagoonee K, Guerois R, Petit MA [PubMed]

Cell Chem Biol. 2019 Sep 18;:
Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1. Authors: Bakail M, Gaubert A, Andreani J, Moal G, Pinna G, Boyarchuk E, Gaillard MC, Courbeyrette R, Mann C, Thuret JY, Guichard B, Murciano B, Richet N, Poitou A, Frederic C, Le Du MH, Agez M, Roelants C, Gurard-Levin ZA, Almouzni G, Cherradi N, Guerois R, Ochsenbein F [PubMed]

Prog Biophys Mol Biol. 2015 Oct;119(1):1
Editorial. Protein-Protein Interaction Analysis and Modulation. Authors: Ochsenbein F, Morelli X, Verma C [PubMed]

Bioinformatics. 2015 Dec 1;31(23):3850-2
HHalign-Kbest : exploring sub-optimal alignments for remote homology comparative modeling. Authors: Yu J, Picord G, Tuffery P, Guerois R [PubMed]

J Biol Chem. 2015 Jul 24;290(30):18721-31
New Insights into the Coupling between Microtubule Depolymerization and ATP Hydrolysis by Kinesin-13 Protein Kif2C. Authors: Wang W, Shen T, Guerois R, Zhang F, Kuerban H, Lv Y, Gigant B, Knossow M, Wang C [PubMed]

Bioinformatics. 2015 Jun 1;31(11):1729-37
bbcontacts : prediction of β-strand pairing from direct coupling patterns. Authors: Andreani J, Söding J [PubMed]

Nucleic Acids Res. 2015 Feb 18;43(3):1905-17
Structural insight into how the human helicase subunit MCM2 may act as a histone chaperone together with ASF1 at the replication fork. Authors: Richet N, Liu D, Legrand P, Velours C, Corpet A, Gaubert A, Bakail M, Moal-Raisin G, Guerois R, Compper C, Besle A, Guichard B, Almouzni G, Ochsenbein F [PubMed]

BMC Genomics. 2014;15:1027
Automated classification of tailed bacteriophages according to their neck organization. Authors: Lopes A, Tavares P, Petit MA, Guérois R, Zinn-Justin S [PubMed]

Nucleic Acids Res. 2014 Dec 1;42(21):13174-85
Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit. Authors: Meurisse J, Bacquin A, Richet N, Charbonnier JB, Ochsenbein F, Peyroche A [PubMed]

PLoS Genet. 2019 Oct 04;15(10):e1008441
Histone deposition promotes recombination-dependent replication at arrested forks. Authors: Hardy J, Dai D, Ait Saada A, Teixeira-Silva A, Dupoiron L, Mojallali F, Fréon K, Ochsenbein F, Hartmann B, Lambert S [PubMed]

iScience. 2020 Apr 18;23(5):101042
Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. Authors: Omrane M, Camara AS, Taveneau C, Benzoubir N, Tubiana T, Yu J, Guérois R, Samuel D, Goud B, Poüs C, Bressanelli S, Garratt RC, Thiam AR, Gassama-Diagne A [PubMed]

PLoS Biol. 2014 Oct;12(10):e1001968
The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. Authors: Pietrobon V, Fréon K, Hardy J, Costes A, Iraqui I, Ochsenbein F, Lambert SA [PubMed]

Elife. 2014;3:e03035
A structural model of the active ribosome-bound membrane protein insertase YidC. Authors: Wickles S, Singharoy A, Andreani J, Seemayer S, Bischoff L, Berninghausen O, Soeding J, Schulten K, van der Sluis EO, Beckmann R [PubMed]

Arch Biochem Biophys. 2014 Jul 15;554:65-75
Evolution of protein interactions : from interactomes to interfaces. Authors: Andreani J, Guerois R [PubMed]

J Proteome Res. 2020 Apr 27;:
Probing protein interaction networks by combining MS-based proteomics and structural data integration. Authors: Postic G, Marcoux J, Reys V, Andreani J, VandenBrouck Y, Bousquet MP, Mouton-Barbosa E, Cianférani S, Burlet-Schiltz O, Guerois R, Labesse G, Tuffery P [PubMed]

Sci Rep. 2019 May 15;9(1):7438
Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein. Authors: Genera M, Samson D, Raynal B, Haouz A, Baron B, Simenel C, Guerois R, Wolff N, Caillet-Saguy C [PubMed]

Sci Rep. 2017 Aug 11;7(1):7875
Regulation of the Human Phosphatase PTPN4 by the inter-domain linker connecting the PDZ and the phosphatase domains. Authors: Caillet-Saguy C, Toto A, Guerois R, Maisonneuve P, di Silvio E, Sawyer K, Gianni S, Wolff N [PubMed]

Nat Struct Mol Biol. 2018 Oct;25(10):971-980
XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining. Authors: Nemoz C, Ropars V, Frit P, Gontier A, Drevet P, Yu J, Guerois R, Pitois A, Comte A, Delteil C, Barboule N, Legrand P, Baconnais S, Yin Y, Tadi S, Barbet-Massin E, Berger I, Le Cam E, Modesti M, Rothenberg E, Calsou P, Charbonnier JB [PubMed]

PLoS Genet. 2018 Feb 14;14(2):e1007223
The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes. Authors: Adam C, Guérois R, Citarella A, Verardi L, Adolphe F, Béneut C, Sommermeyer V, Ramus C, Govin J, Couté Y, Borde V [PubMed]

Genes Dev. 2018 Feb 09;:
A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation. Authors: De Muyt A, Pyatnitskaya A, Andréani J, Ranjha L, Ramus C, Laureau R, Fernandez-Vega A, Holoch D, Girard E, Govin J, Margueron R, Couté Y, Cejka P, Guérois R, Borde V [PubMed]

Elife. 2019 May 21;8:
The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore. Authors: Fischböck-Halwachs J, Singh S, Potocnjak M, Hagemann G, Solis-Mezarino V, Woike S, Ghodgaonkar-Steger M, Weissmann F, Gallego LD, Rojas J, Andreani J, Köhler A, Herzog F [PubMed]

Mol Cell. 2017 Mar 06;:
Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes. Authors: Baejen C, Andreani J, Torkler P, Battaglia S, Schwalb B, Lidschreiber M, Maier KC, Boltendahl A, Rus P, Esslinger S, Söding J, Cramer P [PubMed]

Elife. 2017 Mar 18;6:
Insights into the molecular architecture and histone H3-H4 deposition mechanism of yeast Chromatin assembly factor 1. Authors: Sauer PV, Timm J, Liu D, Sitbon D, Boeri-Erba E, Velours C, Mücke N, Langowski J, Ochsenbein F, Almouzni G, Panne D [PubMed]

Bioinformatics. 2016 Aug 22;
PPI4DOCK : Large scale assessment of the use of homology models in free docking over more than 1000 realistic targets. Authors: Yu J, Guerois R [PubMed]

Elife. 2017 Jan 04;6:
Concerted action of the MutLβ heterodimer and Mer3 helicase regulates the global extent of meiotic gene conversion. Authors: Duroc Y, Kumar R, Ranjha L, Adam C, Guérois R, Md Muntaz K, Marsolier-Kergoat MC, Dingli F, Laureau R, Loew D, Llorente B, Charbonnier JB, Cejka P, Borde V [PubMed]

Sci Rep. 2019 Nov 05;9(1):16036
Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif. Authors: Vilela F, Velours C, Chenon M, Aumont-Nicaise M, Campanacci V, Thureau A, Pylypenko O, Andreani J, Llinas P, Ménétrey J [PubMed]

Acta Crystallogr F Struct Biol Commun. 2016 Mar 1;72(Pt 3):198-206
Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement. Authors: Llinas P, Chenon M, Nguyen TQ, Moreira C, de Régibus A, Coquard A, Ramos MJ, Guérois R, Fernandes PA, Ménétrey J [PubMed]

Proteins. 2019 Nov 19;:
Docking Proteins and Peptides Under Evolutionary Constraints in CAPRI rounds 38-45. Authors: Nadaradjane AA, Quignot C, Traoré S, Andreani J, Guerois R [PubMed]

PLoS Comput Biol. 2018 Mar;14(3):e1005992
Meet-U : Educating through research immersion. Authors: Abdollahi N, Albani A, Anthony E, Baud A, Cardon M, Clerc R, Czernecki D, Conte R, David L, Delaune A, Djerroud S, Fourgoux P, Guiglielmoni N, Laurentie J, Lehmann N, Lochard C, Montagne R, Myrodia V, Opuu V, Parey E, Polit L, Privé S, Quignot C, Ruiz-Cuevas M, Sissoko M, Sompairac N, Vallerix A, Verrecchia V, Delarue M, Guérois R, Ponty Y, Sacquin-Mora S, Carbone A, Froidevaux C, Le Crom S, Lespinet O, Weigt M, Abboud S, Bernardes J, Bouvier G, Dequeker C, Ferré A, Fuchs P, Lelandais G, Poulain P, Richard H, Schweke H, Laine E, Lopes A [PubMed]

J Cell Sci. 2016 Sep 2;
Direct interaction between Exocyst and Wave complexes promotes cell protrusions and motility. Authors: Biondini M, Sadou-Dubourgnoux A, Paul-Gilloteaux P, Zago G, Arslanhan MD, Waharte F, Formstecher E, Hertzog M, Yu J, Guerois R, Gautreau A, Scita G, Camonis J, Parrini MC [PubMed]

Plant Cell. 2019 Jul 02;:
An EDS1-SAG101 Complex is Essential for TNL-mediated Immunity in Nicotiana benthamiana. Authors: Gantner J, Ordon J, Kretschmer C, Guerois R, Stuttmann J [PubMed]

J Clin Invest. 2017 Sep 25;:
Mutations in the netrin-1 gene cause congenital mirror movements. Authors: Méneret A, Franz EA, Trouillard O, Oliver TC, Zagar Y, Robertson SP, Welniarz Q, Gardner RJM, Gallea C, Srour M, Depienne C, Jasoni CL, Dubacq C, Riant F, Lamy JC, Morel MP, Guérois R, Andreani J, Fouquet C, Doulazmi M, Vidailhet M, Rouleau GA, Brice A, Chédotal A, Dusart I, Roze E, Markie D [PubMed]

Methods Mol Biol. 2018;1764:429-447
Protein-Protein Docking Using Evolutionary Information. Authors: Nadaradjane AA, Guerois R, Andreani J [PubMed]

Chembiochem. 2018 Dec 04;:
Recognition of ASF1 Using Hydrocarbon Constrained Peptides. Authors: Bakail M, Rodriguez-Marin S, Hegedüs Z, Perrin ME, Ochsenbein F, Wilson AJ [PubMed]

PLoS Genet. 2018 Apr 02;14(4):e1007317
FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination. Authors: Fernandes JB, Duhamel M, Seguéla-Arnaud M, Froger N, Girard C, Choinard S, Solier V, De Winne N, De Jaeger G, Gevaert K, Andrey P, Grelon M, Guerois R, Kumar R, Mercier R [PubMed]

PLoS One. 2017;12(10):e0186354
Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain. Authors: Nguyen TQ, Chenon M, Vilela F, Velours C, Aumont-Nicaise M, Andreani J, Varela PF, Llinas P, Ménétrey J [PubMed]

EMBO Rep. 2018 Apr 09;:
Disentangling the molecular determinants for Cenp-F localization to nuclear pores and kinetochores. Authors: Berto A, Yu J, Morchoisne-Bolhy S, Bertipaglia C, Vallee R, Dumont J, Ochsenbein F, Guerois R, Doye V [PubMed]

Proteins. 2016 Oct 4;:
Lessons from (co-)evolution in the docking of proteins and peptides for CAPRI Rounds 28-35. Authors: Yu J, Andreani J, Ochsenbein F, Guerois R [PubMed]

PLoS One. 2018;13(5):e0197193
Correction : Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain. Authors: Nguyen TQ, Chenon M, Vilela F, Velours C, Aumont-Nicaise M, Andreani J, Varela PF, Llinas P, Ménétrey J [PubMed]

Nucleic Acids Res. 2018 May 08;:
InterEvDock2 : an expanded server for protein docking using evolutionary and biological information from homology models and multimeric inputs. Authors: Quignot C, Rey J, Yu J, Tufféry P, Guerois R, Andreani J [PubMed]

Front Microbiol. 2018;9:743
Sak4 of Phage HK620 Is a RecA Remote Homolog With Single-Strand Annealing Activity Stimulated by Its Cognate SSB Protein. Authors: Hutinet G, Besle A, Son O, McGovern S, Guerois R, Petit MA, Ochsenbein F, Lecointe F [PubMed]

Front Microbiol. 2018;9:3014
Structural Analysis of the Hanks-Type Protein Kinase YabT From Bacillus subtilis Provides New Insights in its DNA-Dependent Activation. Authors: Shi L, Cavagnino A, Rabefiraisana JL, Lazar N, Li de la Sierra-Gallay I, Ochsenbein F, Valerio-Lepiniec M, Urvoas A, Minard P, Mijakovic I, Nessler S [PubMed]

EMBO J. 2018 May 29;:
The trimeric coiled-coil HSBP1 protein promotes WASH complex assembly at centrosomes. Authors: Visweshwaran SP, Thomason PA, Guerois R, Vacher S, Denisov EV, Tashireva LA, Lomakina ME, Lazennec-Schurdevin C, Lakisic G, Lilla S, Molinie N, Henriot V, Mechulam Y, Alexandrova AY, Cherdyntseva NV, Bièche I, Schmitt E, Insall RH, Gautreau A [PubMed]

Proteins. 2016 Apr 28;
Prediction of homo- and hetero-protein complexes by protein docking and template-based modeling : a CASP-CAPRI experiment. Authors: Lensink MF, Velankar S, Kryshtafovych A, Huang SY, Schneidman-Duhovny D, Sali A, Segura J, Fernandez-Fuentes N, Viswanath S, Elber R, Grudinin S, Popov P, Neveu E, Lee H, Baek M, Park S, Heo L, Rie Lee G, Seok C, Qin S, Zhou HX, Ritchie DW, Maigret B, Devignes MD, Ghoorah A, Torchala M, Chaleil RA, Bates PA, Ben-Zeev E, Eisenstein M, Negi SS, Weng Z, Vreven T, Pierce BG, Borrman TM, Yu J, Ochsenbein F, Guerois R, Vangone A, Rodrigues JP, van Zundert G, Nellen M, Xue L, Karaca E, Melquiond AS, Visscher K, Kastritis PL, Bonvin AM, Xu X, Qiu L, Yan C, Li J, Ma Z, Cheng J, Zou X, Shen Y, Peterson LX, Kim HR, Roy A, Han X, Esquivel-Rodriguez J, Kihara D, Yu X, Bruce NJ, Fuller JC, Wade RC, Anishchenko I, Kundrotas PJ, Vakser IA, Imai K, Yamada K, Oda T, Nakamura T, Tomii K, Pallara C, Romero-Durana M, Jiménez-García B, Moal IH, Férnandez-Recio J, Joung JY, Kim JY, Joo K, Lee J, Kozakov D, Vajda S, Mottarella S, Hall DR, Beglov D, Mamonov A, Xia B, Bohnuud T, Del Carpio CA, Ichiishi E, Marze N, Kuroda D, Roy Burman SS, Gray JJ, Chermak E, Cavallo L, Oliva R, Tovchigrechko A, Wodak SJ [PubMed]

Proc Natl Acad Sci U S A. 2019 Jul 19;:
Patronus is the elusive plant securin, preventing chromosome separation by antagonizing separase. Authors: Cromer L, Jolivet S, Singh DK, Berthier F, De Winne N, De Jaeger G, Komaki S, Prusicki MA, Schnittger A, Guérois R, Mercier R [PubMed]

Nucleic Acids Res. 2016 Apr 29;
InterEvDock : a docking server to predict the structure of protein-protein interactions using evolutionary information. Authors: Yu J, Vavrusa M, Andreani J, Rey J, Tufféry P, Guerois R [PubMed]

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Nous rejoindre

Si vous souhaitez rejoindre notre équipe, merci de contacter Françoise Ochsenbein ou Raphaël Guerois.
​Loc​​ation of the laboratory : CEA Saclay

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