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Accueil > Plateformes > Plateformes IMAGERIE-GIF : Cytométrie, Microscopie Electronique & Microscopie Photonique > Publications & Communications

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  • High throughput measurement of recombination rates and genetic interference in S. cerevisiae.

    27 mars, par Raffoux X, Bourge M, Dumas F, Martin OC, Falque M
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    High throughput measurement of recombination rates and genetic interference in S. cerevisiae.

    Yeast. 2018 Mar 25;:

    Authors: Raffoux X, Bourge M, Dumas F, Martin OC, Falque M

    Abstract
    Allelic recombination due to meiotic crossovers is a major driver of genome evolution, as well as a key player for the selection of high-performing genotypes in economically important species. Therefore, we developed a high throughput and low cost method to measure recombination rates and crossover patterning (including interference) in large populations of the budding yeast S. cerevisiae. Recombination and interference were analyzed by flow cytometry, which allows escaping time-consuming steps such as tetrad microdissection or spore growth. Moreover, our method can also be used to compare recombination in wild-type vs mutant individuals or in different environmental conditions, even if the changes in recombination rates are small. Furthermore, meiotic mutants often present recombination and/or pairing defects affecting spore viability but our method does not involve growth steps and thus avoids filtering out non-viable spores.

    PMID: 29577404 [PubMed - as supplied by publisher]

  • The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene.

    31 janvier, par Chaintreuil C, Gully D, Hervouet C, Tittabutr P, Randriambanona H, Brown SC, Lewis GP, Bourge M, Cartieaux F, Boursot M, Ramanankierana H, D'Hont A, Teaumroong N, Giraud E, Arrighi JF
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    The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene.

    New Phytol. 2016 Aug;211(3):1077-91

    Authors: Chaintreuil C, Gully D, Hervouet C, Tittabutr P, Randriambanona H, Brown SC, Lewis GP, Bourge M, Cartieaux F, Boursot M, Ramanankierana H, D'Hont A, Teaumroong N, Giraud E, Arrighi JF

    Abstract
    The legume genus Aeschynomene is notable in the ability of certain semiaquatic species to develop nitrogen-fixing stem nodules. These species are distributed in two clades. In the first clade, all the species are characterized by the use of a unique Nod-independent symbiotic process. In the second clade, the species use a Nod-dependent symbiotic process and some of them display a profuse stem nodulation as exemplified in the African Aeschynomene afraspera. To facilitate the molecular analysis of the symbiotic characteristics of such legumes, we took an integrated molecular and cytogenetic approach to track occurrences of polyploidy events and to analyze their impact on the evolution of the African species of Aeschynomene. Our results revealed two rounds of polyploidy: a paleopolyploid event predating the African group and two neopolyploid speciations, along with significant chromosomal variations. Hence, we found that A. afraspera (8x) has inherited the contrasted genomic properties and the stem-nodulation habit of its parental lineages (4x). This study reveals a comprehensive picture of African Aeschynomene diversification. It notably evidences a history that is distinct from the diploid Nod-independent clade, providing clues for the identification of the specific determinants of the Nod-dependent and Nod-independent symbiotic processes, and for comparative analysis of stem nodulation.

    PMID: 27061605 [PubMed - indexed for MEDLINE]