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Accueil > Départements > Biologie des Génomes > Daniel GAUTHERET : Séquence, Structure et Fonction des ARN

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NCBI : db=pubmed ; Term=(Gautheret D[Author] NOT Dessen P[Author]) OR Leclerc, Fabrice[Full Author Name] OR (Lehmann Jean[Full Author Name] AND (Orsay[AD] OR Gif[AD])) OR Toffano-Nioche C[Author]) AND ("2012"[PDAT] : "2035"[PDAT])

Articles syndiqués

  • Landscape of the non-coding transcriptome response of two Arabidopsis ecotypes to phosphate starvation.

    15 mai, par Blein T, Balzergue C, Roulé T, Gabriel M, Scalisi L, François T, Sorin C, Christ A, Godon C, Delannoy E, Martin-Magniette ML, Nussaume L, Hartmann C, Gautheret D, Desnos TM, Crespi M

    Landscape of the non-coding transcriptome response of two Arabidopsis ecotypes to phosphate starvation.

    Plant Physiol. 2020 May 13;:

    Authors: Blein T, Balzergue C, Roulé T, Gabriel M, Scalisi L, François T, Sorin C, Christ A, Godon C, Delannoy E, Martin-Magniette ML, Nussaume L, Hartmann C, Gautheret D, Desnos TM, Crespi M

    Abstract
    Root architecture varies widely between species, and even between ecotypes of the same species, despite the strong conservation of the coding portion of their genomes. By contrast, non-coding RNAs evolve rapidly between ecotypes and may control their differential responses to the environment, since several long non-coding RNAs (lncRNAs) are known to quantitatively regulate gene expression. Roots from Columbia (Col) and Landsberg erecta (Ler) ecotypes respond differently to phosphate starvation. Here, we compared transcriptomes (mRNAs, lncRNAs, and small RNAs) of root tips from these two ecotypes during early phosphate starvation. We identified thousands of lncRNAs that were largely conserved at the DNA level in these ecotypes. In contrast to coding genes, many lncRNAs were specifically transcribed in one ecotype and/or differentially expressed between ecotypes independent of phosphate availability. We further characterized these ecotype-related lncRNAs and studied their link with siRNAs. Our analysis identified 675 lncRNAs differentially expressed between the two ecotypes, including antisense RNAs targeting key regulators of root-growth responses. Mis-regulation of several intergenic lncRNAs showed that at least two ecotype-related lncRNAs regulate primary root growth in Col. RNA-seq analysis following the deregulation of the lncRNA NPC48 revealed a potential link with root growth and transport functions. This exploration of the non-coding transcriptome identified ecotype-specific lncRNAs-mediated regulation in root apexes. The non-coding genome may harbor further mechanisms involved in ecotype adaptation of roots to different soil environments.

    PMID: 32404413 [PubMed - as supplied by publisher]

  • Ribozyme Chemistry : To Be or Not To Be under High Pressure.

    6 décembre 2019, par Maurel MC, Leclerc F, Hervé G
    Related Articles

    Ribozyme Chemistry: To Be or Not To Be under High Pressure.

    Chem Rev. 2019 Dec 05;:

    Authors: Maurel MC, Leclerc F, Hervé G

    Abstract
    The use of high hydrostatic pressure to investigate structure-function relationships in biomacromolecules in solution provides precise information about conformational changes and variations of the interactions between these macromolecules and the solvent, as well as the volume changes associated with their activity. The complementary use of osmotic pressure reveals quantitatively the extent and direction of the water exchanges between the macromolecules and the solvent and the number of water molecules involved in these exchanges. In this review, the chemistry of ribozymes and the influence of pressure is described. In the case of the hairpin ribozyme, pressure slowed down the self-cleavage reaction on the basis that the formation of the transition state involves a positive ΔV⧧ of activation and the release of 78 ± 4 water molecules. The self-cleaving activity of the hammerhead ribozyme is also slowed down by pressure on the basis of kinetic parameters and ΔVs comparable to those of the hairpin ribozymes. However, it appears that the solution of the hammerhead ribozyme used in this study contains two populations of molecules which differ by the values of these parameters. The results obtained in the case of small self-cleaving ribozymes containing adenine bulges are consistent with the hypothesis that these small RNAs that bind amino acids or peptides could have appeared in prebiotic chemistry under extreme conditions in deep-sea vents or hydrothermal surface sites.

    PMID: 31804075 [PubMed - as supplied by publisher]