Publis Equipe Legouis – Institute for Integrative Biology of the Cell

Autophagy, Stress and Development

Publications

2021

Chen Y, Leboutet R, Largeau C, Zentout S, Lefebvre C, Delahodde A, Culetto E, Legouis R. Autophagy facilitates mitochondrial rebuilding after acute heat stress via a DRP-1-dependent process. J Cell Biol. 2021 Apr 5;220(4).

Klionsky DJ, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy. 2021 Jan;17(1):1-382.

2020

Leboutet R, Chen Y, Legouis R*, Culetto E*. Mitophagy during development and stress in C. elegans. Mech Ageing Dev. 2020 May 23;189:111266.111266. Review

Prigent M., Chaillot J., Tisserand A., Boy-Marcotte E., Cuif M.H.. Three members of the yeast N-BAR proteins family form heterogeneous lattices in vivo and interact differentially with two RabGAP proteins. Sci. Rep., 2020, vol. 10 1698.

2019

Largeau C, Culetto E, Legouis R. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative Light Electron Microscopy. Methods Mol Biol. 2019;1998:49-61.

Largeau C, Legouis R. Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo. Methods Mol Biol. 2019;1880:281-293.

R LEGOUIS and E. CULETTO are editors of the Methods Mol Biol. 2019- vol 1998 “ESCRT complexes”.

Jenzer C, Simionato E, LARGEAU C, Scarcelli V, Lefebvre C, Legouis R. Autophagy mediates phosphatidylserine exposure and phagosome degradation during apoptosis through specific functions of GABARAP/LGG-1 and LC3/LGG-2. Autophagy. 2019 Feb;15(2):228-241.

2018

Lefebvre C, Legouis R*, Culetto E*. ESCRT and autophagies: Endosomal functions and beyond. Semin Cell Dev Biol. 2018 Feb;74:21-28. *Last Co-authors. Review

2017

Chen Y, Scarcelli V, Legouis R. Approaches for Studying Autophagy in Caenorhabditis elegans. Cells. 2017 Aug 30;6(3). Review

Stringari C, Abdeladim L, Malkinson G, Mahou P, Solinas X, Lamarre I, Brizion S, Galey JB, Supatto W, Legouis R, Pena AM, Beaurepaire E. Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing. Sci Rep. 2017 Jun 19;7(1):3792.

Jenzer C, Legouis R. Multiple functions of autophagy during development. Med Sci (Paris). 2017 Mar;33(3):238-245. Review

2016

Yi Z, Manil-Ségalen M, Sago L, Glatigny A, Redeker V, Legouis R*, Mucchielli-Giorgi MH*. SAFER, an Analysis Method of Quantitative Proteomic Data, Reveals New Interactors of the C. elegans Autophagic Protein LGG-1. J Proteome Res. 2016 May 6;15(5):1515-23.

Lefebvre C, Largeau C, Michelet X, Fourrage C, Maniere X, Matic I, Legouis R*, Culetto E. The ESCRT-II proteins are involved in shaping the sarcoplasmic reticulum in C. elegans. J Cell Sci. 2016 Apr 1;129(7):1490-9.

2015

Zhang H, Chang JT, Guo B, Hansen M, Jia K, Kovács AL, Kumsta C, Lapierre LR, Legouis R, Lin L, Lu Q, Meléndez A, O’Rourke EJ, Sato K, Sato M, Wang X, Wu F. Guidelines for monitoring autophagy in Caenorhabditis elegans. Autophagy. 2015;11(1):9-27.

Jenzer C, Simionato E, Legouis R. Tools and methods to analyze autophagy in C. elegans. Methods. 2015 Mar;75:162-71.

2021

Klionsky, Daniel J., Amal Kamal Abdel-Aziz, Sara Abdelfatah, Mahmoud Abdellatif, Asghar Abdoli, Steffen Abel, Hagai Abeliovich, et al. 2021. “Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (4th Edition).” Autophagy, February, 1–382. https://doi.org/10.1080/15548627.2020.1797280.

Chen, Yanfang, Romane Leboutet, Céline Largeau, Siham Zentout, Christophe Lefebvre, Agnès Delahodde, Emmanuel Culetto, and Renaud Legouis. 2021. “Autophagy Facilitates Mitochondrial Rebuilding after Acute Heat Stress via a DRP-1-Dependent Process.” The Journal of Cell Biology 220 (4): e201909139. https://doi.org/10.1083/jcb.201909139.

Chen, Yanfang, Emmanuel Culetto, and Renaud Legouis. 2021. “A DRP-1 Dependent Autophagy Process Facilitates Rebuilding of the Mitochondrial Network and Modulates Adaptation Capacity in Response to Acute Heat Stress during C. Elegans Development.” Autophagy, August, 1–2. https://doi.org/10.1080/15548627.2021.1953821.

2020

Leboutet, Romane, Yanfang Chen, Renaud Legouis, and Emmanuel Culetto. 2020. “Mitophagy during Development and Stress in C. Elegans.” Mechanisms of Ageing and Development 189 (July): 111266. https://doi.org/10.1016/j.mad.2020.111266.

2019

Le Bars, Romain, Michele W. Bianchi, and Christophe Lefebvre. 2019. “Three-Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software.” In The ESCRT Complexes: Methods and Protocols, edited by Emmanuel Culetto and Renaud Legouis, 149–61. Methods in Molecular Biology. New York, NY: Springer. https://doi.org/10.1007/978-1-4939-9492-2_11.

Largeau, Céline, and Renaud Legouis. 2019. “Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis Elegans Embryo.” In Autophagy: Methods and Protocols, edited by Nicholas Ktistakis and Oliver Florey, 281–93. Methods in Molecular Biology. New York, NY: Springer. https://doi.org/10.1007/978-1-4939-8873-0_18.

Largeau, Céline, Emmanuel Culetto, and Renaud Legouis. 2019. “Subcellular Localization of ESCRT-II in the Nematode C. Elegans by Correlative Light Electron Microscopy.” In The ESCRT Complexes: Methods and Protocols, edited by Emmanuel Culetto and Renaud Legouis, 49–61. Methods in Molecular Biology. New York, NY: Springer. https://doi.org/10.1007/978-1-4939-9492-2_4.

Jenzer, Celine, Elena Simionato, Celine Largeau, Vincent Scarcelli, Christophe Lefebvre, and Renaud Legouis. 2019. “Autophagy Mediates Phosphatidylserine Exposure and Phagosome Degradation during Apoptosis through Specific Functions of GABARAP/LGG-1 and LC3/LGG-2.” Autophagy 15 (2): 228–41. https://doi.org/10.1080/15548627.2018.1512452.

Morizet, Josephine, Guillaume Ducourthial, Willy Supatto, Arthur Boutillon, Renaud Legouis, Marie-Claire Schanne-Klein, Chiara Stringari, and Emmanuel Beaurepaire. 2019. “High-Speed Polarization-Resolved Third-Harmonic Microscopy.” Optica 6 (3): 385–88. https://doi.org/10.1364/OPTICA.6.000385.

2018

Lefebvre, Christophe, Renaud Legouis, and Emmanuel Culetto. 2018. “ESCRT and Autophagies: Endosomal Functions and Beyond.” Seminars in Cell & Developmental Biology 74: 21–28. https://doi.org/10.1016/j.semcdb.2017.08.014.

2017

Jenzer, Céline, and Renaud Legouis. 2017. “Les Multiples Facettes de l’autophagie Au Cours Du Développement.” Médecine/Sciences 33 (3): 238–45. https://doi.org/10.1051/medsci/20173303009.

Stringari, Chiara, Lamiae Abdeladim, Guy Malkinson, Pierre Mahou, Xavier Solinas, Isabelle Lamarre, Sébastien Brizion, et al. 2017. “Multicolor Two-Photon Imaging of Endogenous Fluorophores in Living Tissues by Wavelength Mixing.” Scientific Reports 7 (1): 3792. https://doi.org/10.1038/s41598-017-03359-8.

Chen, Yanfang, Vincent Scarcelli, and Renaud Legouis. 2017. “Approaches for Studying Autophagy in Caenorhabditis Elegans.” Cells 6 (3): 27. https://doi.org/10.3390/cells6030027.

2016

Klionsky, Daniel J., Kotb Abdelmohsen, Akihisa Abe, Md Joynal Abedin, Hagai Abeliovich, Abraham Acevedo Arozena, Hiroaki Adachi, et al. 2016. “Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (3rd Edition).” Autophagy 12 (1): 1–222. https://doi.org/10.1080/15548627.2015.1100356.

Lefebvre, Christophe, Céline Largeau, Xavier Michelet, Cécile Fourrage, Xavier Maniere, Ivan Matic, Renaud Legouis, and Emmanuel Culetto. 2016. “The ESCRT-II Proteins Are Involved in Shaping the Sarcoplasmic Reticulum in C. Elegans.” Journal of Cell Science 129 (7): 1490–99. https://doi.org/10.1242/jcs.178467.

Yi, Zhou, Marion Manil-Ségalen, Laila Sago, Annie Glatigny, Virginie Redeker, Renaud Legouis, and Marie-Hélène Mucchielli-Giorgi. 2016. “SAFER, an Analysis Method of Quantitative Proteomic Data, Reveals New Interactors of the C. Elegans Autophagic Protein LGG-1.” Journal of Proteome Research 15 (5): 1515–23. https://doi.org/10.1021/acs.jproteome.5b01158.

2015

Zhang, Hong, Jessica T. Chang, Bin Guo, Malene Hansen, Kailiang Jia, Attila L. Kovács, Caroline Kumsta, et al. 2015. “Guidelines for Monitoring Autophagy in Caenorhabditis Elegans.” Autophagy 11 (1): 9–27. https://doi.org/10.1080/15548627.2014.1003478.

Jenzer, Céline, Elena Simionato, and Renaud Legouis. 2015. “Tools and Methods to Analyze Autophagy in C. Elegans.” Methods 75: 162–71. https://doi.org/10.1016/j.ymeth.2014.11.019.

2014

Jenzer C, Manil-Ségalen M, LEFEBVRE C, LARGEAU C, Glatigny A, Legouis R. Human GABARAP can restore autophagosome biogenesis in a C. elegans lgg-1 mutant. Autophagy. 2014 Oct 1;10:1868-72.

Manil-Ségalen M, LEFEBVRE C, Jenzer C, Trichet M, Boulogne C, Satiat-Jeunemaitre B, Legouis R. The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39. Dev Cell. 2014 Jan 13;28(1):43-55.

Manil-Ségalen M, CULETTO E, Legouis R*, LEFEBVRE C*. Interactions between endosomal maturation and autophagy: analysis of ESCRT machinery during Caenorhabditis elegans development. Methods Enzymol. 2014;534:93-118. *Last Co-authors

2012

Djeddi A, Michelet X, Culetto E, Alberti A, Barois N, Legouis R. Induction of autophagy in ESCRT mutants is an adaptive response for cell survival in C. elegans. J Cell Sci. 2012 Feb 1;125(Pt 3):685-94.

Prigent M., Boy-Marcotte E., Chesneau L., Dupré-Crochet S., Gibson K., Tisserand H., Verbavatz J.M., Cuif M.H. The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic, 2011, vol.12, 1084-1097.

2011

Al Rawi S, Louvet-Vallée S, Djeddi A, Sachse M, Culetto E, Hajjar C, Boyd L, Legouis R.*, Galy V*. Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission. Science. 2011 Nov 25;334(6059):1144-7

2010

Alberti A, Michelet X, Djeddi A, Legouis R. The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans. Autophagy. 2010 Jul;6(5):622-33.