Autophagy and Development



Chen Y, Leboutet R, Largeau C, Zentout S, Lefebvre C, Delahodde A, Culetto E,  Legouis R. Autophagy facilitates mitochondrial rebuilding after acute heat stress via a DRP-1-dependent process. J Cell Biol. 2021 Apr 5;220(4).

Klionsky DJ, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy. 2021 Jan;17(1):1-382.



Leboutet R, Chen Y, Legouis R*, Culetto E*. Mitophagy during development and stress in C. elegans. Mech Ageing Dev. 2020 May 23;189:111266.111266. Review


Prigent M., Chaillot J., Tisserand A., Boy-Marcotte E., Cuif M.H.. Three members of the yeast N-BAR proteins family form heterogeneous lattices in vivo and interact differentially with two RabGAP proteins. Sci. Rep., 2020,  vol. 10 1698.


Largeau C, Culetto E,  Legouis R. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative Light Electron Microscopy. Methods Mol Biol. 2019;1998:49-61.

Largeau C, Legouis R. Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo. Methods Mol Biol. 2019;1880:281-293.

R LEGOUIS and E. CULETTO are editors of the Methods Mol Biol. 2019- vol 1998 “ESCRT complexes”.

Jenzer C, Simionato E, LARGEAU C, Scarcelli V, Lefebvre C,  Legouis R. Autophagy mediates phosphatidylserine exposure and phagosome degradation during apoptosis through specific functions of GABARAP/LGG-1 and LC3/LGG-2. Autophagy. 2019 Feb;15(2):228-241.


Lefebvre C, Legouis R*, Culetto E*. ESCRT and autophagies: Endosomal functions and beyond. Semin Cell Dev Biol. 2018 Feb;74:21-28. *Last Co-authors. Review


Chen Y, Scarcelli V, Legouis R. Approaches for Studying Autophagy in Caenorhabditis elegans. Cells. 2017 Aug 30;6(3). Review

Stringari C, Abdeladim L, Malkinson G, Mahou P, Solinas X, Lamarre I, Brizion  S, Galey JB, Supatto W, Legouis R, Pena AM, Beaurepaire E. Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing. Sci Rep. 2017 Jun 19;7(1):3792.

Jenzer C, Legouis R. Multiple functions of autophagy during development. Med Sci (Paris). 2017 Mar;33(3):238-245. Review


Yi Z, Manil-Ségalen M, Sago L, Glatigny A, Redeker V, Legouis R*, Mucchielli-Giorgi MH*. SAFER, an Analysis Method of Quantitative Proteomic Data, Reveals New Interactors of the C. elegans Autophagic Protein LGG-1. J Proteome Res. 2016 May 6;15(5):1515-23.

Lefebvre C, Largeau C, Michelet X, Fourrage C, Maniere X, Matic I, Legouis R*, Culetto E. The ESCRT-II proteins are involved in shaping the sarcoplasmic reticulum in C. elegans. J Cell Sci. 2016 Apr 1;129(7):1490-9.


Zhang H, Chang JT, Guo B, Hansen M, Jia K, Kovács AL, Kumsta C, Lapierre LR,  Legouis R, Lin L, Lu Q, Meléndez A, O’Rourke EJ, Sato K, Sato M, Wang X, Wu F. Guidelines for monitoring autophagy in Caenorhabditis elegans. Autophagy. 2015;11(1):9-27.

Jenzer C, Simionato E, Legouis R. Tools and methods to analyze autophagy in C. elegans. Methods. 2015 Mar;75:162-71.


Jenzer C, Manil-Ségalen M, LEFEBVRE C, LARGEAU C, Glatigny A, Legouis R. Human GABARAP can restore autophagosome biogenesis in a C. elegans lgg-1 mutant. Autophagy. 2014 Oct 1;10:1868-72.

Manil-Ségalen M, LEFEBVRE C, Jenzer C, Trichet M, Boulogne C, Satiat-Jeunemaitre B, Legouis R. The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39. Dev Cell. 2014 Jan 13;28(1):43-55.

Manil-Ségalen M, CULETTO E, Legouis R*, LEFEBVRE C*. Interactions between endosomal maturation and autophagy: analysis of ESCRT machinery during Caenorhabditis elegans development. Methods Enzymol. 2014;534:93-118. *Last Co-authors


Djeddi A, Michelet X, Culetto E, Alberti A, Barois N, Legouis R. Induction of autophagy in ESCRT mutants is an adaptive response for cell survival in C. elegans. J Cell Sci. 2012 Feb 1;125(Pt 3):685-94.

Prigent M., Boy-Marcotte E., Chesneau L., Dupré-Crochet S., Gibson K., Tisserand H., Verbavatz J.M., Cuif M.H. The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic, 2011, vol.12, 1084-1097.


Al Rawi S, Louvet-Vallée S, Djeddi A, Sachse M, Culetto E, Hajjar C, Boyd L, Legouis R.*, Galy V*. Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission. Science. 2011 Nov 25;334(6059):1144-7


Alberti A, Michelet X, Djeddi A, Legouis R. The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans. Autophagy. 2010 Jul;6(5):622-33.

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