I2BC publishes 150
articles and reviews a year
Latest Publications
3888256
i2bc
1
chicago-author-date
10
date
desc
year
4613
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22G9BYRPDS%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bo%5Cu00ebffard-Dosierre%20et%20al.%22%2C%22parsedDate%22%3A%222026-12-31%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BBo%26%23xEB%3Bffard-Dosierre%2C%20Liza%2C%20Camille%20Dagallier%2C%20G%26%23xE9%3Braldine%20Eudier%2C%20et%20al.%202026.%20%26%23x201C%3BNMR%20Detects%20Clustering%20and%20Ultra-Weak%20Excipient%20Interactions%20Governing%20Monoclonal%20Antibody%20Viscosity%20in%20Formulation-Relevant%20Conditions.%26%23x201D%3B%20%26lt%3Bi%26gt%3BmAbs%26lt%3B%5C%2Fi%26gt%3B%2018%20%281%29%3A%202685366.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F19420862.2026.2685366%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F19420862.2026.2685366%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22NMR%20detects%20clustering%20and%20ultra-weak%20excipient%20interactions%20governing%20monoclonal%20antibody%20viscosity%20in%20formulation-relevant%20conditions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Liza%22%2C%22lastName%22%3A%22Bo%5Cu00ebffard-Dosierre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Dagallier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G%5Cu00e9raldine%22%2C%22lastName%22%3A%22Eudier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vasco%22%2C%22lastName%22%3A%22Filipe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Atifa%22%2C%22lastName%22%3A%22Badar-Majeed%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sol%5Cu00e8ne%22%2C%22lastName%22%3A%22Fraumont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Zinn-Justin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Xavier%22%2C%22lastName%22%3A%22Theillet%22%7D%5D%2C%22abstractNote%22%3A%22High-concentration%20formulations%20of%20monoclonal%20antibodies%20%28mAbs%29%20are%20required%20for%20subcutaneous%20administration%20but%20are%20frequently%20challenging%20to%20develop%20due%20to%20elevated%20viscosity%20and%20colloidal%20instability.%20These%20properties%20are%20governed%20by%20mAb-mAb%20interactions%20that%20are%20regulated%20by%20additional%20mAb-excipient%20interactions.%20These%20ultra-weak%20interactions%20remain%20difficult%20to%20characterize%20using%20conventional%20techniques.%20Here%2C%20we%20combined%20nuclear%20magnetic%20resonance%20%28NMR%29%20spectroscopy%20with%20rheometry%20and%20dynamic%20light%20scattering%20to%20investigate%20mAb%20interactions%20under%20formulation-relevant%20conditions.%20Rheological%20measurements%20showed%20that%2C%20in%20the%20case%20of%20the%20chosen%20mAb%2C%20the%20excipients%20arginine%20and%20lysine%20strongly%20reduce%20the%20macroscopic%20viscosity%2C%20whereas%20other%20excipients%20display%20a%20moderate%20effect%20by%20themselves.%20Characterization%20of%20the%20mAb%20oligomeric%20state%20by%201H%20NMR%20confirmed%20that%20arginine%20and%20lysine%20are%20the%20most%20efficient%20at%20reducing%20mAb%20self-assembly%2C%20while%20proline%20and%20glycine%20promote%20clustering.%20Interactions%20between%20mAb%20and%20excipients%20were%20first%20analyzed%20by%20measuring%20excipient%20diffusion%20coefficients%2C%20but%20these%20are%20only%20weakly%20affected%20by%20the%20addition%20of%20concentrated%20mAb.%20The%20mAb-excipient%20interactions%20were%20further%20detected%20by%20measuring%20excipient%201H%20and%2013C%20chemical%20shifts%2C%20mAb-excipient%20saturation%20transfers%2C%20and%20excipient%201H%20transverse%20relaxation%20rates.%20These%20experiments%20provided%20complementary%20information%20on%20excipient%20interactions%20with%20the%20different%20mAb%20oligomers.%20Lysine%20was%20identified%20as%20the%20best%20mAb%20binder.%20However%2C%20several%20excipients%20such%20as%20sucrose%20that%20do%20not%20reduce%20the%20macroscopic%20viscosity%20also%20bind%20to%20concentrated%20mAb%2C%20highlighting%20that%20excipient%20binding%20can%20have%20various%20consequences%20on%20the%20transient%20interactions%20between%20mAb%20species.%20Altogether%2C%20this%20work%20proposes%20a%20powerful%20NMR%20pipeline%20to%20dissect%20ultra-weak%20molecular%20interactions%20that%20govern%20viscosity%20and%20developability%20in%20therapeutic%20antibody%20formulations.%22%2C%22date%22%3A%222026-12-31%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1080%5C%2F19420862.2026.2685366%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242272436%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221942-0870%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-06-12T07%3A26%3A39Z%22%7D%7D%2C%7B%22key%22%3A%22MIKI9GE2%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Golubewa%20et%20al.%22%2C%22parsedDate%22%3A%222026-09-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BGolubewa%2C%20Lena%2C%20Yaraslau%20Padrez%2C%20Marius%20Franckevi%26%23x10D%3Bius%2C%20et%20al.%202026.%20%26%23x201C%3BMapping%20the%20Energy%20Transfer%20Network%20in%20the%20Diatom%20Cyclotella%20Meneghiniana%20by%20Picosecond%20Time-Resolved%20Fluorescence%20Spectroscopy%20down%20to%20Cryogenic%20Temperatures.%26%23x201D%3B%20%26lt%3Bi%26gt%3BThe%20Journal%20of%20Physical%20Chemistry%20Letters%26lt%3B%5C%2Fi%26gt%3B%2017%20%2835%29%3A%2010191%26%23x2013%3B98.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jpclett.6c02173%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jpclett.6c02173%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mapping%20the%20Energy%20Transfer%20Network%20in%20the%20Diatom%20Cyclotella%20meneghiniana%20by%20Picosecond%20Time-Resolved%20Fluorescence%20Spectroscopy%20down%20to%20Cryogenic%20Temperatures%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lena%22%2C%22lastName%22%3A%22Golubewa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yaraslau%22%2C%22lastName%22%3A%22Padrez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marius%22%2C%22lastName%22%3A%22Franckevi%5Cu010dius%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricija%22%2C%22lastName%22%3A%22Zemaityte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Streckait%5Cu0117%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jevgenij%22%2C%22lastName%22%3A%22Chmeliov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leonas%22%2C%22lastName%22%3A%22Valkunas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claudia%22%2C%22lastName%22%3A%22B%5Cu00fcchel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrius%22%2C%22lastName%22%3A%22Gelzinis%22%7D%5D%2C%22abstractNote%22%3A%22Diatoms%20are%20unicellular%20photosynthetic%20microalgae%20that%20contribute%20nearly%20a%20quarter%20of%20the%20global%20primary%20production.%20Knowledge%20of%20the%20mechanisms%2C%20networks%2C%20and%20time%20scales%20of%20the%20excitation%20transfer%20is%20crucial%20for%20a%20deeper%20understanding%20of%20their%20photosynthetic%20machinery.%20Unfortunately%2C%20the%20short%20excited-state%20lifetime%20of%20photosystem%20I%20at%20room%20temperature%20and%20research%20focus%20on%20isolated%20structures%20have%20limited%20the%20development%20of%20a%20complete%20excitation%20transfer%20scheme%20in%20intact%20cells.%20Here%2C%20we%20address%20this%20gap%20through%20picosecond%20time-resolved%20fluorescence%20measurements%20on%20intact%20Cyclotella%20meneghiniana%20cells%20obtained%20from%2016%20to%20130%20K%20and%20room%20temperature.%20Analysis%20of%20the%20data%20in%20terms%20of%20evolution-associated%20spectra%20reveals%20a%20complex%20interplay%20of%20excitation%20transfer%2C%20population%20of%20fluorescing%20trapping%20states%2C%20and%20excitation%20quenching%20at%20the%20whole-cell%20level.%20We%20show%20rapid%20depopulation%20of%20all%20antenna%20complexes%2C%20on%20the%20order%20of%2030%20ps%20even%20at%2016%20K%2C%20indicating%20a%20more%20efficient%20energy-transfer%20network%20in%20diatoms%20than%20in%20higher%20plants.%22%2C%22date%22%3A%222026-09-03%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.jpclett.6c02173%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242691346%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221948-7185%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-09-04T08%3A01%3A18Z%22%7D%7D%2C%7B%22key%22%3A%22MPR44RL6%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Montoliu-Nerin%20et%20al.%22%2C%22parsedDate%22%3A%222026-09-01%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMontoliu-Nerin%2C%20Merce%2C%20Anton%20Strunov%2C%20Eleanor%20Heyworth%2C%20et%20al.%202026.%20%26%23x201C%3BEvolutionary%20Persistence%20of%20a%20Highly%20Prevalent%20Multicopy%20Mitochondrial-Derived%20Nuclear%20Insertion%20%28Mega-NUMT%29%20in%20Neotropical%20Drosophila%20Flies.%26%23x201D%3B%20%26lt%3Bi%26gt%3BMolecular%20Biology%20and%20Evolution%26lt%3B%5C%2Fi%26gt%3B%2C%20September%201%2C%20msag227.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fmolbev%5C%2Fmsag227%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fmolbev%5C%2Fmsag227%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Evolutionary%20persistence%20of%20a%20highly%20prevalent%20multicopy%20mitochondrial-derived%20nuclear%20insertion%20%28Mega-NUMT%29%20in%20Neotropical%20Drosophila%20flies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Merce%22%2C%22lastName%22%3A%22Montoliu-Nerin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anton%22%2C%22lastName%22%3A%22Strunov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eleanor%22%2C%22lastName%22%3A%22Heyworth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniela%20I.%22%2C%22lastName%22%3A%22Schneider%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Thoma%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aur%5Cu00e9lie%22%2C%22lastName%22%3A%22Hua-Van%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9cile%22%2C%22lastName%22%3A%22Courret%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lisa%22%2C%22lastName%22%3A%22Klasson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wolfgang%20J.%22%2C%22lastName%22%3A%22Miller%22%7D%5D%2C%22abstractNote%22%3A%22Although%20strict%20maternal%20transmission%20of%20mitochondria%20is%20a%20general%20feature%20of%20animals%20for%20ensuring%20homogeneity%20in%20mitochondrial%20DNA%20%28mtDNA%29%20across%20generations%2C%20exceptions%20were%20reported%20in%20the%20recent%20past.%20For%20example%2C%20some%20extremely%20rare%20but%20spectacular%20cases%20of%20heteroplasmy%20and%20paternal%20transmission%20in%20humans%20have%20questioned%20the%20universal%20evolutionary%20principle.%20Hence%2C%20as%20an%20alternative%2C%20the%20Mega-NUMT%20concept%20was%20coined%20to%20explain%20this%20discovery%20and%20was%20thereafter%20partly%20proven%20to%20exist.%20This%20concept%20expands%20on%20the%20quite%20common%20transfer%20of%20mtDNA%20fragments%20to%20the%20nucleus%20%28NUMTs%29%20by%20considering%20the%20existence%20of%20multicopy%20mitochondrial%20nuclear%20insertions.%20Mega-NUMT%20reports%20are%20currently%20restricted%20to%20a%20few%20cases%20in%20animals%2C%20including%20humans.%20However%2C%20their%20detailed%20genomic%20organization%2C%20natural%20prevalence%2C%20and%20potential%20biological%20functions%20remain%20unclear.%20Here%2C%20we%20discovered%20that%20up%20to%2060%20full-sized%20mitochondrial%20genomes%20are%20integrated%20into%20the%20nuclear%20genome%20of%20the%20neotropical%20Drosophila%20paulistorum%20using%20long-read%20sequencing%20and%20in%20situ%20hybridization.%20The%20copies%20are%20organized%20in%20one%20cluster%20on%20chromosome%203%2C%20which%20we%20designated%20the%20%26quot%3BDpau%20Mega-NUMT%26quot%3B.%20Contrary%20to%20the%20rarity%20in%20humans%2C%20this%20Mega-NUMT%20is%20found%20at%20high%20prevalence%20%2840%25%29%20in%20both%20laboratory%20lines%20and%20natural%20D.%20paulistorum%20populations%20of%20different%20semispecies.%20Additionally%2C%20the%20Mega-NUMT%20copies%20are%20phylogenetically%20separated%20from%20the%20current%20mitotypes%20of%20D.%20paulistorum.%20Together%2C%20these%20observations%20suggest%20long-term%20maintenance%20of%20the%20Mega-NUMT%20in%20nature.%20Hence%2C%20we%20propose%20that%20the%20Dpau%20Mega-NUMT%20may%20have%20been%20transferred%20to%20the%20nuclear%20genome%20before%20the%20D.%20paulistorum%20semispecies%20radiation%20and%20speculate%20based%20on%20these%20findings%20that%20it%20possibly%20is%20maintained%20at%20relatively%20high%20prevalence%20in%20nature%20by%20balancing%20selection%20or%20due%20to%20a%20yet%20undetermined%20function.%22%2C%22date%22%3A%222026-09-01%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fmolbev%5C%2Fmsag227%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242679361%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221537-1719%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-09-02T07%3A33%3A49Z%22%7D%7D%2C%7B%22key%22%3A%22FIITM8CB%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Melnyk%20et%20al.%22%2C%22parsedDate%22%3A%222026-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMelnyk%2C%20Oleg%2C%20St%26%23xE9%3Bphanie%20Caby%2C%20Armelle%20Vigouroux%2C%20et%20al.%202026.%20%26%23x201C%3BTotal%20Synthesis%20and%20Structural%20Characterization%20of%20a%20Novel%20Protein%20Scaffold%20from%20the%20Snail%20Biomphalaria%20Glabrata.%26%23x201D%3B%20%26lt%3Bi%26gt%3BProtein%20Science%3A%20A%20Publication%20of%20the%20Protein%20Society%26lt%3B%5C%2Fi%26gt%3B%2035%20%289%29%3A%20e70745.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fpro.70745%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fpro.70745%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Total%20synthesis%20and%20structural%20characterization%20of%20a%20novel%20protein%20scaffold%20from%20the%20snail%20Biomphalaria%20glabrata%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Oleg%22%2C%22lastName%22%3A%22Melnyk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phanie%22%2C%22lastName%22%3A%22Caby%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Armelle%22%2C%22lastName%22%3A%22Vigouroux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%22%2C%22lastName%22%3A%22Demanche%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R%5Cu00e9mi%22%2C%22lastName%22%3A%22Desmet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Magalie%22%2C%22lastName%22%3A%22S%5Cu00e9n%5Cu00e9chal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beno%5Cu00eet%22%2C%22lastName%22%3A%22Snella%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Mougel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Boidin-Wichlacz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aur%5Cu00e9lie%22%2C%22lastName%22%3A%22Parmentier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ugo%22%2C%22lastName%22%3A%22Pasco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonia%22%2C%22lastName%22%3A%22Cantel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Solange%22%2C%22lastName%22%3A%22Mor%5Cu00e9ra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00f4me%22%2C%22lastName%22%3A%22Vicogne%22%7D%5D%2C%22abstractNote%22%3A%22Disulfide-rich%20miniproteins%20constitute%20compact%20and%20highly%20stable%20scaffolds%20of%20growing%20interest%20for%20molecular%20and%20structural%20engineering.%20Schistosomins%20are%20~80-residue%20proteins%20conserved%20across%20gastropods%20that%20form%20a%20long-standing%20orphan%20family%20whose%20structure%20and%20biological%20roles%20have%20remained%20unknown.%20Here%2C%20we%20report%20the%20total%20chemical%20synthesis%20and%20structural%20characterization%20of%20a%20schistosomin%20isoform%20from%20Biomphalaria%20glabrata%2C%20a%20medically%20relevant%20intermediate%20host%20of%20the%20parasite%20Schistosoma%20mansoni.%20Using%20state-of-the-art%20solid-phase%20peptide%20synthesis%2C%20chemoselective%20peptide%20ligation%2C%20and%20controlled%20oxidative%20folding%2C%20we%20obtained%20homogeneous%2C%20well-folded%20schistosomin%20suitable%20for%20biophysical%20and%20structural%20studies.%20High-resolution%20X-ray%20crystallography%20reveals%20a%20previously%20undescribed%20disulfide-rich%20fold%20defining%20a%20new%20class%20of%20miniprotein%20scaffold.%20Nano%20differential%20scanning%20fluorimetry%20and%20circular%20dichroism%20experiments%20demonstrate%20the%20remarkable%20thermal%20stability%20of%20this%20scaffold.%20Complementary%20in%20silico%20analyses%20suggest%20that%20the%20two%20naturally%20occurring%20isoforms%2C%20which%20differ%20by%20a%20single%20residue%2C%20exhibit%20highly%20similar%20structural%20and%20dynamic%20properties.%20Finally%2C%20transcript%20and%20protein%20analyses%20across%20snail%20tissues%20provide%20the%20first%20spatial%20expression%20map%20of%20schistosomin%20in%20a%20medically%20relevant%20mollusk.%20Together%2C%20this%20work%20establishes%20schistosomin%20as%20a%20novel%20and%20robust%20miniprotein%20scaffold%20and%20provides%20a%20structural%20and%20biological%20framework%20for%20exploring%20its%20function%20and%20potential%20applications.%22%2C%22date%22%3A%222026-09%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Fpro.70745%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242554520%22%2C%22PMCID%22%3A%22PMC13440164%22%2C%22ISSN%22%3A%221469-896X%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A37%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22QVANUUGL%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zickler%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-17%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BZickler%2C%20Denise%2C%20Ana-Rita%20Rodrigues%20Neves%2C%20and%20Nancy%20Kleckner.%202026.%20%26%23x201C%3BCohesin%20Release-Factor%20Wapl%20Modulates%20Axis%20and%20Chromatin%20Structure%20to%20Promote%20Conjunction%20and%20Separation%20of%20Meiotic%20Chromosomes.%26%23x201D%3B%20%26lt%3Bi%26gt%3BCell%20Reports%26lt%3B%5C%2Fi%26gt%3B%2045%20%288%29%3A%20117776.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.celrep.2026.117776%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.celrep.2026.117776%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cohesin%20release-factor%20Wapl%20modulates%20axis%20and%20chromatin%20structure%20to%20promote%20conjunction%20and%20separation%20of%20meiotic%20chromosomes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denise%22%2C%22lastName%22%3A%22Zickler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ana-Rita%22%2C%22lastName%22%3A%22Rodrigues%20Neves%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nancy%22%2C%22lastName%22%3A%22Kleckner%22%7D%5D%2C%22abstractNote%22%3A%22Analysis%20of%20meiotic%20prophase%20in%20Sordaria%20macrospora%20shows%20that%20the%20cohesin-release%20factor%20Wapl%20is%20a%20multi-stage%20modulator%20of%20whole-chromosomal%20dynamics.%20Absence%20of%20Wapl%20affects%20chromosome%20lengths%2C%20but%20without%20direct%20effects%20on%20basic%20recombination%20events%20or%20synaptonemal%20complex%20%28SC%29%20formation%20and%20removal%2C%20implying%20that%20the%20fundamental%20axis%20structure%20remains%20intact.%20Instead%2C%20Wapl%20is%20%281%29%20required%20for%20regular%20juxtaposition%20of%20homolog%5C%2Fpartner%20axes%20in%20space%2C%20raising%20the%20possibility%20that%20cohesin%20might%20directly%20mediate%20this%20process%3B%20%282%29%20important%20for%20telomere%20dynamics%20during%20the%20bouquet%20stage%20and%20for%20chromosome-entanglement%20resolution%3B%20and%20%283%29%20required%20for%20correct%20homolog%20segregation.%20Wapl%20also%20mediates%20axis%20and%20chromatin%20disorganization%20during%20the%20transit%20from%20SC%20removal%20to%20diplotene%20%28the%20%26quot%3Bdiffuse%20stage%26quot%3B%29%20but%2C%20nonetheless%2C%20allows%20chromosome%20reformation%20into%20normal%20diplotene%5C%2Fmetaphase%20states%2C%20begging%20the%20question%20of%20why%20the%20diffuse%20stage%20exists%20at%20all.%20Notably%2C%20Wapl%20modulates%20not%20only%20the%20axis%20state%20but%20also%2C%20separately%2C%20the%20chromatin%20state%2C%20pointing%20to%20direct%20and%5C%2For%20indirect%20role%28s%29%20for%20cohesin%20in%20that%20aspect.%22%2C%22date%22%3A%222026-08-17%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.celrep.2026.117776%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242611704%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222211-1247%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-31T07%3A46%3A57Z%22%7D%7D%2C%7B%22key%22%3A%22NYMDHZJL%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Tsydenzhapova%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-13%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BTsydenzhapova%2C%20Ekaterina%2C%20Artem%20I.%20Fokin%2C%20Nicolas%20B.%20David%2C%20et%20al.%202026.%20%26%23x201C%3BThe%20Nance-Horan%20Syndrome%20Protein%20NHS%20Regulates%20Cell%20Migration%20Persistence%20by%20Organizing%20WAVE%20and%20N-WASP%20Nucleation-Promoting%20Factor%20Complexes.%26%23x201D%3B%20%26lt%3Bi%26gt%3BThe%20Journal%20of%20Biological%20Chemistry%26lt%3B%5C%2Fi%26gt%3B%2C%20August%2013%2C%20113443.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2026.113443%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2026.113443%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Nance-Horan%20syndrome%20protein%20NHS%20regulates%20cell%20migration%20persistence%20by%20organizing%20WAVE%20and%20N-WASP%20nucleation-promoting%20factor%20complexes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ekaterina%22%2C%22lastName%22%3A%22Tsydenzhapova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Artem%20I.%22%2C%22lastName%22%3A%22Fokin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolas%20B.%22%2C%22lastName%22%3A%22David%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathalie%22%2C%22lastName%22%3A%22Rocques%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Polesskaya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Iman%22%2C%22lastName%22%3A%22Haddad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jo%5Cu00eblle%22%2C%22lastName%22%3A%22Vinh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Gu%5Cu00e9rois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexis%20M.%22%2C%22lastName%22%3A%22Gautreau%22%7D%5D%2C%22abstractNote%22%3A%22The%20function%20of%20the%20NHS%20gene%20that%20is%20responsible%20for%20the%20Nance-Horan%20Syndrome%20has%20remained%20elusive%20at%20the%20cellular%20level.%20Using%20CRISPR%5C%2FCas9%2C%20we%20inactivated%20the%20NHS%20gene%20in%20MCF10A%20cells%20and%20characterized%20the%20isoforms%20expressed%20in%20these%20cells.%20NHS%20KO%20cells%20displayed%20reduced%20migration%20persistence%2C%20a%20phenotype%20that%20was%20fully%20rescued%20by%20the%20long%20isoform%201%20%28i1%29%20that%20contains%20a%20N-terminal%20WAVE%20Homology%20Domain%20%28WHD%29%2C%20but%20only%20partially%20rescued%20by%20the%20short%20isoform%202%20%28i2%29%2C%20which%20does%20not.%20Patient%20mutations%20resulting%20in%20NHS%20proteins%20truncated%20at%20their%20C-terminus%20also%20reduced%20the%20ability%20of%20NHS%20i1%20to%20rescue%20migration%20persistence.%20Using%20Tandem%20Affinity%20Purification%20%28TAP%29%20of%20NHS%20i1%20and%20mass%20spectrometry%2C%20we%20identified%20as%20major%20NHS%20partners%2C%20all%20subunits%20of%20the%20WAVE%20Regulatory%20Complex%20%28WRC%29%20except%20WAVE%20subunits%20themselves%2C%20indicating%20that%20the%20WHD%20of%20NHS%20assembles%20a%20WAVE%20Shell%20Complex%20%28WSC%29.%20The%20Arp2%5C%2F3%20complex%20and%20the%20Nucleation%20Promoting%20Factor%20%28NPF%29%20WAVE%20that%20activates%20it%20are%20critical%20for%20migration%20persistence.%20To%20investigate%20the%20role%20of%20the%20NHS-containing%20WSC%2C%20we%20performed%20TAP%20of%20the%20ABI1%20subunit%20in%20parental%20and%20NHS%20KO%20cells%20and%20identified%20differential%20partners%20associated%20with%20ABI1%20only%20in%20parental%20cells%2C%20but%20not%20in%20NHS%20KO%20cells.%20The%20most%20abundant%20of%20these%20were%20the%20WIPF2%5C%2FN-WASP%20complex%2C%20which%20together%20with%20the%20kinase%20ABL2%2C%20was%20also%20critical%20for%20migration%20persistence.%20These%20results%20suggest%20that%20NHS%20controls%20cell%20migration%20by%20remodeling%20NPF%20complexes%20and%20their%20higher%20order%20assembly.%22%2C%22date%22%3A%222026-08-13%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jbc.2026.113443%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242595116%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221083-351X%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A38%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22XGPR22QM%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Romero%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BRomero%2C%20Juan%20J.%2C%20Roxanne%20Bercy%2C%20Viola%20C.%20D%26%23x2019%3BMello%2C%20et%20al.%202026.%20%26%23x201C%3BDependence%20of%20the%20Excited-State%20Manifold%20of%20Lutein%20on%20Polarisability.%26%23x201D%3B%20%26lt%3Bi%26gt%3BPhysical%20Chemistry%20Chemical%20Physics%3A%20PCCP%26lt%3B%5C%2Fi%26gt%3B%2028%20%2831%29%3A%2018922%26%23x2013%3B30.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fd6cp01897a%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2Fd6cp01897a%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Dependence%20of%20the%20excited-state%20manifold%20of%20lutein%20on%20polarisability%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juan%20J.%22%2C%22lastName%22%3A%22Romero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roxanne%22%2C%22lastName%22%3A%22Bercy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viola%20C.%22%2C%22lastName%22%3A%22D%27Mello%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%22%2C%22lastName%22%3A%22Gall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristian%22%2C%22lastName%22%3A%22Ilioaia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20A.%22%2C%22lastName%22%3A%22Pascal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manuel%20J.%22%2C%22lastName%22%3A%22Llansola-Portoles%22%7D%5D%2C%22abstractNote%22%3A%22Lutein%20is%20a%20xanthophyll%20carotenoid%20with%20a%20conjugated%20system%20terminated%20by%20two%20ionone%20rings%2C%20one%20of%20which%20extends%20the%20%5Cu03c0-conjugation.%20Its%20photophysical%20behaviour%20in%20different%20environments%20is%20poorly%20understood.%20Here%2C%20we%20report%20room-temperature%20excited-state%20characterisation%20of%20lutein%20in%20two%20solvents%20with%20strongly%20different%20polarisabilities%2C%20THF%20and%20CS2%2C%20combining%20femtosecond%20transient%20absorption%20and%20femtosecond%20stimulated%20resonance%20Raman%20spectroscopy.%20In%20both%20environments%2C%20we%20resolve%20the%20same%20set%20of%20excited%20states%2C%20including%20a%20hot%20S1%20state%2C%20relaxed%20S1%2C%20a%20weak%20ICT%20contribution%20coupled%20to%20S1%2C%20and%20a%20longer-lived%20dark%20state%20with%20triplet-pair%20character%2C%20S%2A.%20Despite%20this%20shared%20state%20manifold%2C%20the%20two%20solvents%20produce%20markedly%20different%20behaviours.%20In%20CS2%2C%20the%20S1%20absorption%20band%20is%20substantially%20red-shifted%20and%20broadened%20relative%20to%20THF%2C%20and%20the%20vibrational%20dynamics%20of%20the%20excited-state%20manifold%20evolve%20much%20less%20over%20time%2C%20indicating%20that%20highly%20polarisable%20environments%20suppress%20relaxation-driven%20spectral%20evolution.%20The%20S%2A%20dark%20state%20lifetime%20of%20%5Cu223c16%20ps%20in%20THF%20increases%20to%20approximately%2029%20ps%20in%20CS2%2C%20and%20its%20vibrational%20frequency%20shifts%20upward%20by%208%20cm-1%2C%20deviating%20from%20the%20scaling%20observed%20for%20linear%20carotenoids%20of%20comparable%20conjugation%20length.%22%2C%22date%22%3A%222026-08-12%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1039%5C%2Fd6cp01897a%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242478512%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221463-9084%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A45%3A39Z%22%7D%7D%2C%7B%22key%22%3A%22HYQV4PZ7%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Li%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-12%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BLi%2C%20Bo%2C%20Marie%20E.%20Perrin%2C%20Emma%20Maillard%2C%20et%20al.%202026.%20%26%23x201C%3BDownsizing%20the%20Histone%20H3-H4%20Quaternary%20Structure%20Into%20Foldamer%20Mimetics%20Yields%20High-Affinity%20and%20Cell-Permeable%20Ligands%20of%20ASF1.%26%23x201D%3B%20%26lt%3Bi%26gt%3BAngewandte%20Chemie%26lt%3B%5C%2Fi%26gt%3B%20%28International%20ed.%20in%20English%29%2C%20August%2012%2C%20e4112426.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.4112426%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.4112426%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Downsizing%20the%20Histone%20H3-H4%20Quaternary%20Structure%20Into%20Foldamer%20Mimetics%20Yields%20High-Affinity%20and%20Cell-Permeable%20Ligands%20of%20ASF1%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bo%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%20E.%22%2C%22lastName%22%3A%22Perrin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emma%22%2C%22lastName%22%3A%22Maillard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arthur%22%2C%22lastName%22%3A%22Vitard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gwena%5Cu00eblle%22%2C%22lastName%22%3A%22Moal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johanne%22%2C%22lastName%22%3A%22Mbianda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Andr%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Magda%22%2C%22lastName%22%3A%22Teixeira%20Nunes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Vandamme%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Pinna%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Douat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Thinon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Guerois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Legrand%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Guichard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francoise%22%2C%22lastName%22%3A%22Ochsenbein%22%7D%5D%2C%22abstractNote%22%3A%22Mimicking%20complex%20protein-protein%20interfaces%20with%20small%2C%20well-defined%20molecular%20scaffolds%20remains%20a%20major%20challenge%20in%20chemical%20biology.%20Here%2C%20we%20report%20a%20foldamer-based%20downsizing%20strategy%20that%20compresses%20the%20quaternary%20architecture%20of%20the%20histone%20H3-H4%20dimer%20into%20compact%20peptide-oligourea%20hybrids%20acting%20as%20high-affinity%20ligands%20of%20the%20histone%20chaperone%20Anti-Silencing%20Function%201%20%28ASF1%29.%20Guided%20by%20multiple%20high-resolution%20co-crystal%20structures%2C%20we%20designed%20a%20series%20of%20foldamer%20mimetics%20that%20accurately%20reproduce%20both%20the%20H3%20%5Cu03b1-helix%20and%20the%20H4%20%5Cu03b2-strand%20epitopes.%20Systematic%20optimization%20of%20linker%20geometry%2C%20%5Cu03b2-strand%20mimicry%2C%20formal%20charge%2C%20and%20selective%20backbone%20N-methylation%20yielded%20highly%20stable%20ligands%20with%20nanomolar%20affinities%2C%20enhanced%20proteolytic%20resistance%2C%20and%20robust%20cytosolic%20penetration.%20Notably%2C%20the%20optimized%20constructs%20and%20their%20N-methylated%20analogues%20recapitulate%20the%20binding%20mode%20of%20the%20native%20H3-H4%20dimer%20on%20ASF1%20with%20high%20fidelity%20and%20engage%20endogenous%20ASF1%20in%20cell%20extracts%2C%20demonstrating%20effective%20intracellular%20target%20recognition.%20Together%2C%20these%20results%20show%20that%20peptide-oligourea%20foldamers%20can%20reproduce%20the%20structural%20features%20of%20a%20protein%20quaternary%20structure%20surface%2C%20combining%20high%20affinity%2C%20high%20stability%20and%20cell%20permeability.%22%2C%22date%22%3A%222026-08-12%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1002%5C%2Fanie.4112426%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242585141%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221521-3773%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A38%3A55Z%22%7D%7D%2C%7B%22key%22%3A%22AGW5VEXD%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Jayachandran%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-10%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BJayachandran%2C%20Akshay%2C%20Martin%20M%26%23xFC%3Btze%2C%20Kostiantyn%20Romaniuk%2C%20et%20al.%202026.%20%26%23x201C%3BHuman%20PIF1%20Clears%20Secondary%20DNA%20Structures%20by%20Coupled%20DNA%20Unwinding%20and%20Rewinding%20Activities.%26%23x201D%3B%20%26lt%3Bi%26gt%3BGenes%20%26amp%3B%20Development%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20August%2010.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2Fgad.353775.126%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2Fgad.353775.126%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Human%20PIF1%20clears%20secondary%20DNA%20structures%20by%20coupled%20DNA%20unwinding%20and%20rewinding%20activities%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Akshay%22%2C%22lastName%22%3A%22Jayachandran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22M%5Cu00fctze%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kostiantyn%22%2C%22lastName%22%3A%22Romaniuk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%20J.%20R.%22%2C%22lastName%22%3A%22Hudson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20Rosaria%22%2C%22lastName%22%3A%22Dello%20Stritto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Gu%5Cu00e9rois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ananya%22%2C%22lastName%22%3A%22Acharya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefan%22%2C%22lastName%22%3A%22Braunshier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giordano%22%2C%22lastName%22%3A%22Reginato%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Issam%22%2C%22lastName%22%3A%22Senoussi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ulrich%22%2C%22lastName%22%3A%22Rass%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ralf%22%2C%22lastName%22%3A%22Seidel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Cejka%22%7D%5D%2C%22abstractNote%22%3A%22PIF1%20family%20helicases%20promote%20genome%20stability%20during%20DNA%20replication%20and%20repair%2C%20yet%20the%20biochemical%20activity%20of%20human%20PIF1%20%28hPIF1%29%20remains%20poorly%20understood.%20Here%2C%20we%20directly%20compare%20full-length%20hPIF1%20and%20budding%20yeast%20Pif1%20%28yPif1%29%20and%20show%20that%20the%20two%20enzymes%20operate%20differently.%20In%20bulk%20assays%2C%20hPIF1%20displays%20weak%20net%20DNA%20unwinding%20because%20its%20robust%20intrinsic%20single-stranded%20DNA%20%28ssDNA%29%20annealing%20activity%20rapidly%20reforms%20duplex%20DNA%20behind%20the%20helicase%20motor.%20Single-molecule%20magnetic%20tweezers%20experiments%20reveal%20that%20hPIF1%20is%20only%20modestly%20slower%20than%20yPif1%20and%20similarly%20processive%2C%20indicating%20that%20poor%20apparent%20unwinding%20primarily%20reflects%20rapid%20reannealing%20rather%20than%20impaired%20motor%20activity.%20Mutagenesis%20and%20structural%20modeling%20indicate%20that%20this%20functional%20divergence%20depends%20on%20a%20yeast-specific%20insertion%20within%20the%20catalytic%20domain.%20Unlike%20yPif1%2C%20hPIF1%20fails%20to%20stimulate%20Pol%5Cu03b4-dependent%20displacement-loop%20%28D-loop%29%20extension%2C%20arguing%20that%20it%20does%20not%20promote%20DNA%20unwinding%20ahead%20of%20the%20migrating%20bubble.%20However%2C%20similarly%20to%20yPif1%2C%20hPIF1%20likely%20unwinds%20newly%20synthesized%20DNA%20coupled%20with%20DNA%20reannealing%20behind%20the%20migrating%20D-loop.%20hPIF1%20efficiently%20remodels%20intramolecular%20G-quadruplex%20DNA%20into%20fully%20base-paired%20dsDNA%2C%20whereas%20yPif1%20generates%20ssDNA%20products.%20Together%2C%20our%20data%20support%20a%20model%20in%20which%20hPIF1%20acts%20primarily%20as%20a%20DNA%20translocase%20that%20couples%20local%20unwinding%20with%20rapid%20rewinding%20to%20clear%20secondary%20DNA%20structures%20without%20generating%20extensive%20ssDNA.%22%2C%22date%22%3A%222026-08-10%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1101%5C%2Fgad.353775.126%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242575689%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221549-5477%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A41%3A55Z%22%7D%7D%2C%7B%22key%22%3A%22LZAEX9A9%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Le%20Ven%20et%20al.%22%2C%22parsedDate%22%3A%222026-08-10%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BLe%20Ven%2C%20Ana%26%23xEF%3Bs%2C%20Sandra%20Vanhuele%2C%20Olivier%20Ganier%2C%20et%20al.%202026.%20%26%23x201C%3BHuman%20PMS1-Dependent%20Non-Canonical%20Mismatch%20Repair%20Engages%20with%20MBD4%20to%20Repair%20Methylated%20CpG%20Deamination.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNucleic%20Acids%20Research%26lt%3B%5C%2Fi%26gt%3B%2054%20%2815%29%3A%20gkag771.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkag771%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkag771%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Human%20PMS1-dependent%20non-canonical%20mismatch%20repair%20engages%20with%20MBD4%20to%20repair%20methylated%20CpG%20deamination%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ana%5Cu00efs%22%2C%22lastName%22%3A%22Le%20Ven%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sandra%22%2C%22lastName%22%3A%22Vanhuele%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Ganier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%22%2C%22lastName%22%3A%22Houy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amanda%22%2C%22lastName%22%3A%22Kahn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manuel%22%2C%22lastName%22%3A%22Rodrigues%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc-Henri%22%2C%22lastName%22%3A%22Stern%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Raphael%22%2C%22lastName%22%3A%22Guerois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andr%5Cu00e9%20Bortolini%22%2C%22lastName%22%3A%22Silveira%22%7D%5D%2C%22abstractNote%22%3A%22CpG%20dinucleotides%20are%20mutational%20hotspots%20due%20to%20spontaneous%20deamination%20of%205-methylcytosine%20%285mC%29%2C%20resulting%20in%20T%3AG%20mismatches%20that%20can%20lead%20to%20CpG%26gt%3BTpG%20transitions.%20These%20mutations%20are%20a%20hallmark%20of%20aging%20and%20cancer%20and%20play%20a%20central%20role%20in%20the%20evolution%20of%20vertebrate%20genomes.%20We%20have%20previously%20uncovered%20MBD4%20as%20the%20primary%20base%20excision%20repair%20%28BER%29%20glycosylase%20responsible%20for%205mC%20deamination%20repair.%20Here%2C%20we%20employ%20an%20APOBEC1%20deaminase%20fused%20to%20a%20catalytically%20dead%20Cas9%20to%20induce%20targeted%205mC%20deamination%20independently%20of%20DNA%20replication%20and%20track%20its%20repair%20in%20human%20cells.%20This%20approach%20reveals%20that%20MBD4%20elicits%20a%20coordinated%20repair%20response%20with%20a%20non-canonical%20branch%20of%20mismatch%20repair%20%28MMR%29%20involving%20complexes%20MutL%5Cu03b2%20%28MLH1-PMS1%29%20and%20MutS%5Cu03b1%20%28MSH2-MSH6%29.%20We%20uncover%20the%20physical%20interaction%20between%20MBD4%20and%20MutL%5Cu03b2%20and%20demonstrate%20that%20MBD4-mediated%20repair%20requires%20MLH1.%20We%20show%20that%20PMS1%20deficiency%20phenocopies%20the%20CpG%26gt%3BTpG%20hypermutation%20signature%20characteristic%20of%20MBD4%20loss%2C%20establishing%205mC%20deamination%20repair%20as%20a%20key%20function%20of%20human%20PMS1.%20In%20alignment%20with%20our%20experimental%20data%2C%20we%20show%20that%20the%20CpG%26gt%3BTpG%20mutational%20burden%20in%20MMR-deficient%20tumors%20is%20partly%20explained%20by%20replication-independent%20processes.%20Altogether%2C%20we%20uncover%20a%20novel%20function%20of%20non-canonical%20MMR%20that%20underscores%20its%20interplay%20with%20BER%20in%20safeguarding%20genomic%20integrity%20against%20damage%20to%20methylated%20DNA.%22%2C%22date%22%3A%222026-08-10%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkag771%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242578366%22%2C%22PMCID%22%3A%22PMC13458396%22%2C%22ISSN%22%3A%221362-4962%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-08-24T11%3A40%3A00Z%22%7D%7D%5D%7D
Boëffard-Dosierre, Liza, Camille Dagallier, Géraldine Eudier, et al. 2026. “NMR Detects Clustering and Ultra-Weak Excipient Interactions Governing Monoclonal Antibody Viscosity in Formulation-Relevant Conditions.” mAbs 18 (1): 2685366. https://doi.org/10.1080/19420862.2026.2685366.
Golubewa, Lena, Yaraslau Padrez, Marius Franckevičius, et al. 2026. “Mapping the Energy Transfer Network in the Diatom Cyclotella Meneghiniana by Picosecond Time-Resolved Fluorescence Spectroscopy down to Cryogenic Temperatures.” The Journal of Physical Chemistry Letters 17 (35): 10191–98. https://doi.org/10.1021/acs.jpclett.6c02173.
Montoliu-Nerin, Merce, Anton Strunov, Eleanor Heyworth, et al. 2026. “Evolutionary Persistence of a Highly Prevalent Multicopy Mitochondrial-Derived Nuclear Insertion (Mega-NUMT) in Neotropical Drosophila Flies.” Molecular Biology and Evolution, September 1, msag227. https://doi.org/10.1093/molbev/msag227.
Melnyk, Oleg, Stéphanie Caby, Armelle Vigouroux, et al. 2026. “Total Synthesis and Structural Characterization of a Novel Protein Scaffold from the Snail Biomphalaria Glabrata.” Protein Science: A Publication of the Protein Society 35 (9): e70745. https://doi.org/10.1002/pro.70745.
Zickler, Denise, Ana-Rita Rodrigues Neves, and Nancy Kleckner. 2026. “Cohesin Release-Factor Wapl Modulates Axis and Chromatin Structure to Promote Conjunction and Separation of Meiotic Chromosomes.” Cell Reports 45 (8): 117776. https://doi.org/10.1016/j.celrep.2026.117776.
Tsydenzhapova, Ekaterina, Artem I. Fokin, Nicolas B. David, et al. 2026. “The Nance-Horan Syndrome Protein NHS Regulates Cell Migration Persistence by Organizing WAVE and N-WASP Nucleation-Promoting Factor Complexes.” The Journal of Biological Chemistry, August 13, 113443. https://doi.org/10.1016/j.jbc.2026.113443.
Romero, Juan J., Roxanne Bercy, Viola C. D’Mello, et al. 2026. “Dependence of the Excited-State Manifold of Lutein on Polarisability.” Physical Chemistry Chemical Physics: PCCP 28 (31): 18922–30. https://doi.org/10.1039/d6cp01897a.
Li, Bo, Marie E. Perrin, Emma Maillard, et al. 2026. “Downsizing the Histone H3-H4 Quaternary Structure Into Foldamer Mimetics Yields High-Affinity and Cell-Permeable Ligands of ASF1.” Angewandte Chemie (International ed. in English), August 12, e4112426. https://doi.org/10.1002/anie.4112426.
Jayachandran, Akshay, Martin Mütze, Kostiantyn Romaniuk, et al. 2026. “Human PIF1 Clears Secondary DNA Structures by Coupled DNA Unwinding and Rewinding Activities.” Genes & Development, ahead of print, August 10. https://doi.org/10.1101/gad.353775.126.
Le Ven, Anaïs, Sandra Vanhuele, Olivier Ganier, et al. 2026. “Human PMS1-Dependent Non-Canonical Mismatch Repair Engages with MBD4 to Repair Methylated CpG Deamination.” Nucleic Acids Research 54 (15): gkag771. https://doi.org/10.1093/nar/gkag771.