Institute for Integrative Biology of the Cell
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Salama, Ehab A., Yehia Elgammal, Aruna Wijeratne, Nadia A. Lanman, Sagar M. Utturkar, Atena Farhangian, Jianing Li, Brigitte Meunier, Tony R. Hazbun, and Mohamed N. Seleem. 2024. “Lansoprazole Interferes with Fungal Respiration and Acts Synergistically with Amphotericin B against Multidrug-Resistant Candida Auris.” Emerging Microbes & Infections 13 (1): 2322649. https://doi.org/10.1080/22221751.2024.2322649.
Catalina-Hernández, Èric, Mario López-Martín, David Masnou-Sánchez, Marco Martins, Victor A. Lorenz-Fonfria, Francesc Jiménez-Altayó, Ute A. Hellmich, et al. 2024. “Experimental and Computational Biophysics to Identify Vasodilator Drugs Targeted at TRPV2 Using Agonists Based on the Probenecid Scaffold.” Computational and Structural Biotechnology Journal 23 (December):473–82. https://doi.org/10.1016/j.csbj.2023.12.028.
Labidi, Raphaël J., Bruno Faivre, Philippe Carpentier, Julien Perard, Philipp Gotico, Yun Li, Mohamed Atta, and Marc Fontecave. 2024. “Light-Activated Artificial CO2-Reductase: Structure and Activity.” Journal of the American Chemical Society, October. https://doi.org/10.1021/jacs.4c08927.
Brünje, Annika, Magdalena Füßl, Jürgen Eirich, Jean-Baptiste Boyer, Paulina Heinkow, Ulla Neumann, Minna Konert, et al. 2024. “The Plastidial Protein Acetyltransferase GNAT1 Forms a Complex with GNAT2, yet Their Interaction Is Dispensable for State Transitions.” Molecular & Cellular Proteomics: MCP, September, 100850. https://doi.org/10.1016/j.mcpro.2024.100850.
Pingault, Véronique, Cécilia Neiva-Vaz, Judite de Oliveira, Núria Martínez-Gil, Amaia Lasa-Aranzasti, Berta Campos, Inge M. M. Lakeman, et al. 2024. “Chromatin Assembly Factor Subunit CHAF1A as a Monogenic Cause for Oculo-Auriculo-Vertebral Spectrum.” European Journal of Human Genetics: EJHG, September. https://doi.org/10.1038/s41431-024-01698-5.
Touroutine, Denis, and Nadya Morozova. 2024. “A Novel Hypothesis about Mechanism of Thalidomide Action on Pattern Formation.” BioSystems, September, 105344. https://doi.org/10.1016/j.biosystems.2024.105344.
Hani, Umama, Belen Naranjo, Ginga Shimakawa, Christophe Espinasse, Hélène Vanacker, Pierre Sétif, Eevi Rintamäki, Emmanuelle Issakidis-Bourguet, and Anja Krieger-Liszkay. 2024. “A Complex and Dynamic Redox Network Regulates Oxygen Reduction at Photosystem I in Arabidopsis.” Plant Physiology, September, kiae501. https://doi.org/10.1093/plphys/kiae501.
Eirich, Jürgen, Jean-Baptiste Boyer, Laura Armbruster, Aiste Ivanauskaite, Carolina De La Torre, Thierry Meinnel, Markus Wirtz, Paula Mulo, Iris Finkemeier, and Carmela Giglione. 2024. “Light Changes Promote Distinct Responses of Plastid Protein Acetylation Marks.” Molecular & Cellular Proteomics: MCP, September, 100845. https://doi.org/10.1016/j.mcpro.2024.100845.
Mathis, Hugues, Delphine Naquin, Antoine Margeot, and Frederique Bidard. 2024. “Enhanced Heterologous Gene Expression in Trichoderma Reesei by Promoting Multicopy Integration.” Applied Microbiology and Biotechnology 108 (1): 470. https://doi.org/10.1007/s00253-024-13308-x.
Mezzetti, Alberto, Winfried Leibl, Jeanette A. Johnson, and J. Thomas Beatty. 2024. “Monitoring Molecular Events during Photo-Driven Ubiquinone Pool Reduction in PufX+ and PufX- Membranes from Rhobobacter Capsulatus by Time-Resolved FTIR Difference Spectroscopy.” Plant Physiology and Biochemistry: PPB 216 (September):109139. https://doi.org/10.1016/j.plaphy.2024.109139.