Institute for Integrative Biology of the Cell
Publications
0
Publications / year
0
different journals
0
fields
0
Publications / year
0
different journals
0
fields
Latest Publications
3888256
i2bc
items
1
chicago-author-date
10
year
desc
year
4613
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-77657764769bcdf738acf57387acdac8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22HJEL7A6J%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Salama%20et%20al.%22%2C%22parsedDate%22%3A%222024-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESalama%2C%20Ehab%20A.%2C%20Yehia%20Elgammal%2C%20Aruna%20Wijeratne%2C%20Nadia%20A.%20Lanman%2C%20Sagar%20M.%20Utturkar%2C%20Atena%20Farhangian%2C%20Jianing%20Li%2C%20Brigitte%20Meunier%2C%20Tony%20R.%20Hazbun%2C%20and%20Mohamed%20N.%20Seleem.%202024.%20%26%23x201C%3BLansoprazole%20Interferes%20with%20Fungal%20Respiration%20and%20Acts%20Synergistically%20with%20Amphotericin%20B%20against%20Multidrug-Resistant%20Candida%20Auris.%26%23x201D%3B%20%3Ci%3EEmerging%20Microbes%20%26amp%3B%20Infections%3C%5C%2Fi%3E%2013%20%281%29%3A%202322649.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F22221751.2024.2322649%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F22221751.2024.2322649%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Lansoprazole%20interferes%20with%20fungal%20respiration%20and%20acts%20synergistically%20with%20amphotericin%20B%20against%20multidrug-resistant%20Candida%20auris%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ehab%20A.%22%2C%22lastName%22%3A%22Salama%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yehia%22%2C%22lastName%22%3A%22Elgammal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aruna%22%2C%22lastName%22%3A%22Wijeratne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nadia%20A.%22%2C%22lastName%22%3A%22Lanman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sagar%20M.%22%2C%22lastName%22%3A%22Utturkar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Atena%22%2C%22lastName%22%3A%22Farhangian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jianing%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brigitte%22%2C%22lastName%22%3A%22Meunier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tony%20R.%22%2C%22lastName%22%3A%22Hazbun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohamed%20N.%22%2C%22lastName%22%3A%22Seleem%22%7D%5D%2C%22abstractNote%22%3A%22Candida%20auris%20has%20emerged%20as%20a%20problematic%20fungal%20pathogen%20associated%20with%20high%20morbidity%20and%20mortality.%20Amphotericin%20B%20%28AmB%29%20is%20the%20most%20effective%20antifungal%20used%20to%20treat%20invasive%20fungal%20candidiasis%2C%20with%20resistance%20rarely%20observed%20among%20clinical%20isolates.%20However%2C%20C.%20auris%20possesses%20extraordinary%20resistant%20profiles%20against%20all%20available%20antifungal%20drugs%2C%20including%20AmB.%20In%20our%20pursuit%20of%20potential%20solutions%2C%20we%20screened%20a%20panel%20of%20727%20FDA-approved%20drugs.%20We%20identified%20the%20proton%20pump%20inhibitor%20lansoprazole%20%28LNP%29%20as%20a%20potent%20enhancer%20of%20AmB%27s%20activity%20against%20C.%20auris.%20LNP%20also%20potentiates%20the%20antifungal%20activity%20of%20AmB%20against%20other%20medically%20important%20species%20of%20Candida%20and%20Cryptococcus.%20Our%20investigations%20into%20the%20mechanism%20of%20action%20unveiled%20that%20LNP%20metabolite%28s%29%20interact%20with%20a%20crucial%20target%20in%20the%20mitochondrial%20respiratory%20chain%20%28complex%20III%2C%20known%20as%20cytochrome%20bc1%29.%20This%20interaction%20increases%20oxidative%20stress%20within%20fungal%20cells.%20Our%20results%20demonstrated%20the%20critical%20role%20of%20an%20active%20respiratory%20function%20in%20the%20antifungal%20activity%20of%20LNP.%20Most%20importantly%2C%20LNP%20restored%20the%20efficacy%20of%20AmB%20in%20an%20immunocompromised%20mouse%20model%2C%20resulting%20in%20a%201.7-log%20%28%5Cu223c98%25%29%20CFU%20reduction%20in%20the%20burden%20of%20C.%20auris%20in%20the%20kidneys.%20Our%20findings%20strongly%20advocate%20for%20a%20comprehensive%20evaluation%20of%20LNP%20as%20a%20cytochrome%20bc1%20inhibitor%20for%20combating%20drug-resistant%20C.%20auris%20infections.%22%2C%22date%22%3A%222024-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1080%5C%2F22221751.2024.2322649%22%2C%22ISSN%22%3A%222222-1751%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-03-04T09%3A45%3A28Z%22%7D%7D%2C%7B%22key%22%3A%22QB7P7VX9%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Catalina-Hern%5Cu00e1ndez%20et%20al.%22%2C%22parsedDate%22%3A%222024-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECatalina-Hern%26%23xE1%3Bndez%2C%20%26%23xC8%3Bric%2C%20Mario%20L%26%23xF3%3Bpez-Mart%26%23xED%3Bn%2C%20David%20Masnou-S%26%23xE1%3Bnchez%2C%20Marco%20Martins%2C%20Victor%20A.%20Lorenz-Fonfria%2C%20Francesc%20Jim%26%23xE9%3Bnez-Altay%26%23xF3%3B%2C%20Ute%20A.%20Hellmich%2C%20et%20al.%202024.%20%26%23x201C%3BExperimental%20and%20Computational%20Biophysics%20to%20Identify%20Vasodilator%20Drugs%20Targeted%20at%20TRPV2%20Using%20Agonists%20Based%20on%20the%20Probenecid%20Scaffold.%26%23x201D%3B%20%3Ci%3EComputational%20and%20Structural%20Biotechnology%20Journal%3C%5C%2Fi%3E%2023%20%28December%29%3A%20473%26%23x2013%3B82.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.csbj.2023.12.028%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.csbj.2023.12.028%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Experimental%20and%20computational%20biophysics%20to%20identify%20vasodilator%20drugs%20targeted%20at%20TRPV2%20using%20agonists%20based%20on%20the%20probenecid%20scaffold%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22%5Cu00c8ric%22%2C%22lastName%22%3A%22Catalina-Hern%5Cu00e1ndez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mario%22%2C%22lastName%22%3A%22L%5Cu00f3pez-Mart%5Cu00edn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Masnou-S%5Cu00e1nchez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marco%22%2C%22lastName%22%3A%22Martins%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Victor%20A.%22%2C%22lastName%22%3A%22Lorenz-Fonfria%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francesc%22%2C%22lastName%22%3A%22Jim%5Cu00e9nez-Altay%5Cu00f3%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ute%20A.%22%2C%22lastName%22%3A%22Hellmich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hitoshi%22%2C%22lastName%22%3A%22Inada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonio%22%2C%22lastName%22%3A%22Alcaraz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuji%22%2C%22lastName%22%3A%22Furutani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alfons%22%2C%22lastName%22%3A%22Nonell-Canals%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jose%20Luis%22%2C%22lastName%22%3A%22V%5Cu00e1zquez-Ibar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carmen%22%2C%22lastName%22%3A%22Domene%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rachelle%22%2C%22lastName%22%3A%22Gaudet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alex%22%2C%22lastName%22%3A%22Per%5Cu00e1lvarez-Mar%5Cu00edn%22%7D%5D%2C%22abstractNote%22%3A%22TRP%20channels%20are%20important%20pharmacological%20targets%20in%20physiopathology.%20TRPV2%20plays%20distinct%20roles%20in%20cardiac%20and%20neuromuscular%20function%2C%20immunity%2C%20and%20metabolism%2C%20and%20is%20associated%20with%20pathologies%20like%20muscular%20dystrophy%20and%20cancer.%20However%2C%20TRPV2%20pharmacology%20is%20unspecific%20and%20scarce%20at%20best.%20Using%20in%20silico%20similarity-based%20chemoinformatics%20we%20obtained%20a%20set%20of%20270%20potential%20hits%20for%20TRPV2%20categorized%20into%20families%20based%20on%20chemical%20nature%20and%20similarity.%20Docking%20the%20compounds%20on%20available%20rat%20TRPV2%20structures%20allowed%20the%20clustering%20of%20drug%20families%20in%20specific%20ligand%20binding%20sites.%20Starting%20from%20a%20probenecid%20docking%20pose%20in%20the%20piperlongumine%20binding%20site%20and%20using%20a%20Gaussian%20accelerated%20molecular%20dynamics%20approach%20we%20have%20assigned%20a%20putative%20probenecid%20binding%20site.%20In%20parallel%2C%20we%20measured%20the%20EC50%20of%207%20probenecid%20derivatives%20on%20TRPV2%20expressed%20in%20Pichia%20pastoris%20using%20a%20novel%20medium-throughput%20Ca2%2B%20influx%20assay%20in%20yeast%20membranes%20together%20with%20an%20unbiased%20and%20unsupervised%20data%20analysis%20method.%20We%20found%20that%204-%28piperidine-1-sulfonyl%29-benzoic%20acid%20had%20a%20better%20EC50%20than%20probenecid%2C%20which%20is%20one%20of%20the%20most%20specific%20TRPV2%20agonists%20to%20date.%20Exploring%20the%20TRPV2-dependent%20anti-hypertensive%20potential%20in%20vivo%2C%20we%20found%20that%204-%28piperidine-1-sulfonyl%29-benzoic%20acid%20shows%20a%20sex-biased%20vasodilator%20effect%20producing%20larger%20vascular%20relaxations%20in%20female%20mice.%20Overall%2C%20this%20study%20expands%20the%20pharmacological%20toolbox%20for%20TRPV2%2C%20a%20widely%20expressed%20membrane%20protein%20and%20orphan%20drug%20target.%22%2C%22date%22%3A%222024-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.csbj.2023.12.028%22%2C%22ISSN%22%3A%222001-0370%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-01-26T08%3A53%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22NE2YJ9VD%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Oumerri%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-22%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EOumerri%2C%20Jihad%2C%20Hicham%20Qayouh%2C%20Ana%20Andreea%20Arteni%2C%20Jean%20Luc%20Six%2C%20Mohammed%20Lahcini%2C%20and%20Khalid%20Ferji.%202024.%20%26%23x201C%3BOne-Pot%20Formulation%20of%20Cationic%20Oligochitosan%20Coated%20Nanoparticles%20via%20Photo-%20Polymerization%20Induced%20Self-Assembly.%26%23x201D%3B%20%3Ci%3EChemphyschem%3A%20A%20European%20Journal%20of%20Chemical%20Physics%20and%20Physical%20Chemistry%3C%5C%2Fi%3E%2C%20April%2C%20e202400291.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fcphc.202400291%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fcphc.202400291%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22One-pot%20Formulation%20of%20Cationic%20Oligochitosan%20Coated%20Nanoparticles%20via%20Photo-%20Polymerization%20Induced%20Self-Assembly%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jihad%22%2C%22lastName%22%3A%22Oumerri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hicham%22%2C%22lastName%22%3A%22Qayouh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ana%20Andreea%22%2C%22lastName%22%3A%22Arteni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%20Luc%22%2C%22lastName%22%3A%22Six%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohammed%22%2C%22lastName%22%3A%22Lahcini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Khalid%22%2C%22lastName%22%3A%22Ferji%22%7D%5D%2C%22abstractNote%22%3A%22During%20last%20few%20decades%2C%20oligochitosan%20%28OCS%29-coated%20nanoparticles%20have%20received%20great%20interest%20for%20nanomedicine%2C%20food%20and%20environment%20applications.%20However%2C%20their%20current%20formulation%20techniques%20are%20time-consuming%20with%20multi-synthesis%5C%2Fpurification%20steps%20and%20sometimes%20require%20the%20use%20of%20organic%20solvents%2C%20crosslinkers%20and%20surfactants.%20Herein%2C%20we%20report%20a%20facile%20and%20rapid%20one-pot%20synthesis%20of%20OCS-based%20nanoparticles%20using%20photo-initiated%20reversible%20addition%20fragmentation%20chain%20transfer%20polymerization-induced%20self-assembly%20%28Photo-RAFT%20PISA%29%20under%20UV-irradiation%20at%20room%20temperature.%20To%20achieve%20this%2C%20OCS%20was%20first%20functionalized%20by%20a%20chain%20transfer%20agent%20%28CTA%29%20resulting%20in%20a%20macromolecular%20chain%20transfer%20agent%20%28OCS-CTA%29%2C%20which%20will%20act%20as%20a%20reactive%20electrostatic%5C%2Fsteric%20stabilizer.%20Owing%20to%20its%20UV-sensitivity%2C%20OCS-CTA%20was%20then%20used%20as%20photo-iniferter%20to%20initiate%20the%20polymerization%20of%202-hydroxypropyl%20methacrylate%20%28HPMA%29%20in%20aqueous%20acidic%20buffer%2C%20resulting%20in%20OCS-g-PHPMA%20amphiphilic%20grafted%20copolymers%20which%20self-assemble%20into%20nano-objects.%20Transmission%20electron%20microscopy%20and%20light%20scattering%20analysis%20reveal%20formation%20of%20spherical%20nanostructures.%22%2C%22date%22%3A%222024-04-22%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1002%5C%2Fcphc.202400291%22%2C%22ISSN%22%3A%221439-7641%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-23T10%3A13%3A27Z%22%7D%7D%2C%7B%22key%22%3A%22GIG8IL6R%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Iacobucci%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-19%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EIacobucci%2C%20Ilaria%2C%20Vittoria%20Monaco%2C%20Agn%26%23xE8%3Bs%20Hovasse%2C%20Baptiste%20Dupouy%2C%20Rodrigue%20Keumoe%2C%20Bogdan%20Cichocki%2C%20Mourad%20Elhabiri%2C%20et%20al.%202024.%20%26%23x201C%3BProteomic%20Profiling%20of%20Antimalarial%20Plasmodione%20Using%203-Benz%28o%29Ylmenadione%20Affinity-Based%20Probes.%26%23x201D%3B%20%3Ci%3EChembiochem%3A%20A%20European%20Journal%20of%20Chemical%20Biology%3C%5C%2Fi%3E%2C%20April%2C%20e202400187.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fcbic.202400187%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fcbic.202400187%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Proteomic%20Profiling%20of%20Antimalarial%20Plasmodione%20Using%203-Benz%28o%29ylmenadione%20Affinity-Based%20Probes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ilaria%22%2C%22lastName%22%3A%22Iacobucci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vittoria%22%2C%22lastName%22%3A%22Monaco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agn%5Cu00e8s%22%2C%22lastName%22%3A%22Hovasse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Baptiste%22%2C%22lastName%22%3A%22Dupouy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodrigue%22%2C%22lastName%22%3A%22Keumoe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bogdan%22%2C%22lastName%22%3A%22Cichocki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mourad%22%2C%22lastName%22%3A%22Elhabiri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brigitte%22%2C%22lastName%22%3A%22Meunier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Marc%22%2C%22lastName%22%3A%22Strub%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Monti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarah%22%2C%22lastName%22%3A%22Cianf%5Cu00e9rani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phanie%20A.%22%2C%22lastName%22%3A%22Blandin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%22%2C%22lastName%22%3A%22Schaeffer-Reiss%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisabeth%22%2C%22lastName%22%3A%22Davioud-Charvet%22%7D%5D%2C%22abstractNote%22%3A%22Understanding%20the%20mechanisms%20of%20drug%20action%20in%20malarial%20parasites%20is%20crucial%20for%20the%20development%20of%20new%20drugs%20to%20combat%20infection%20and%20to%20counteract%20drug%20resistance.%20Proteomics%20is%20a%20widely%20used%20approach%20to%20study%20host-pathogen%20systems%20and%20to%20identify%20drug%20protein%20targets.%20Plasmodione%20is%20an%20antiplasmodial%20early-lead%20drug%20exerting%20potent%20activities%20against%20young%20asexual%20and%20sexual%20blood%20stages%20in%20vitro%20with%20low%20toxicity%20to%20host%20cells.%20To%20elucidate%20its%20molecular%20mechanisms%2C%20an%20affinity-based%20protein%20profiling%20%28AfBPP%29%20approach%20was%20applied%20to%20yeast%20and%20P.%20falciparum%20proteomes.%20New%20%28pro-%29AfBPP%20probes%20based%20on%20the%203-benz%28o%29yl-6-fluoro-menadione%20scaffold%20were%20synthesized.%20With%20optimized%20conditions%20of%20both%20photoaffinity%20labeling%20and%20click%20reaction%20steps%2C%20the%20AfBPP%20protocol%20was%20then%20applied%20to%20a%20yeast%20proteome%2C%20yielding%2011%20putative%20drug-protein%20targets.%20Among%20these%2C%20we%20found%20four%20proteins%20associated%20with%20oxidoreductase%20activities%2C%20the%20hypothesized%20type%20of%20targets%20for%20plasmodione%20and%20its%20metabolites%2C%20and%20other%20proteins%20associated%20with%20the%20mitochondria.%20In%20Plasmodium%20parasites%2C%20the%20MS%20analysis%20revealed%2044%20potential%20plasmodione%20targets%20that%20need%20to%20be%20validated%20in%20further%20studies.%20Finally%2C%20the%20localization%20of%20a%203-benzyl-6-fluoromenadione%20AfBPP%20probe%20was%20studied%20in%20the%20subcellular%20structures%20of%20the%20parasite%20at%20the%20trophozoite%20stage.%22%2C%22date%22%3A%222024-04-19%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1002%5C%2Fcbic.202400187%22%2C%22ISSN%22%3A%221439-7633%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-22T06%3A25%3A29Z%22%7D%7D%2C%7B%22key%22%3A%22I7ZBRC4X%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Buggiani%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-18%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBuggiani%2C%20Julia%2C%20Thierry%20Meinnel%2C%20Carmela%20Giglione%2C%20and%20Fr%26%23xE9%3Bd%26%23xE9%3Bric%20Frottin.%202024.%20%26%23x201C%3BAdvances%20in%20Nuclear%20Proteostasis%20of%20Metazoans.%26%23x201D%3B%20%3Ci%3EBiochimie%3C%5C%2Fi%3E%2C%20April%2C%20S0300-9084%2824%2900081-6.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biochi.2024.04.006%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biochi.2024.04.006%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Advances%20in%20nuclear%20proteostasis%20of%20metazoans%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Buggiani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thierry%22%2C%22lastName%22%3A%22Meinnel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carmela%22%2C%22lastName%22%3A%22Giglione%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fr%5Cu00e9d%5Cu00e9ric%22%2C%22lastName%22%3A%22Frottin%22%7D%5D%2C%22abstractNote%22%3A%22The%20proteostasis%20network%20and%20associated%20protein%20quality%20control%20%28PQC%29%20mechanisms%20ensure%20proteome%20functionality%20and%20are%20essential%20for%20cell%20survival.%20A%20distinctive%20feature%20of%20eukaryotic%20cells%20is%20their%20high%20degree%20of%20compartmentalization%2C%20requiring%20specific%20and%20adapted%20proteostasis%20networks%20for%20each%20compartment.%20The%20nucleus%2C%20essential%20for%20maintaining%20the%20integrity%20of%20genetic%20information%20and%20gene%20transcription%2C%20is%20one%20such%20compartment.%20While%20PQC%20mechanisms%20have%20been%20investigated%20for%20decades%20in%20the%20cytoplasm%20and%20the%20endoplasmic%20reticulum%2C%20our%20knowledge%20of%20nuclear%20PQC%20pathways%20is%20only%20emerging.%20Recent%20developments%20in%20the%20field%20have%20underscored%20the%20importance%20of%20spatially%20managing%20aberrant%20proteins%20within%20the%20nucleus.%20Upon%20proteotoxic%20stress%2C%20misfolded%20proteins%20and%20PQC%20effectors%20accumulate%20in%20various%20nuclear%20membrane-less%20organelles.%20Beyond%20bringing%20together%20effectors%20and%20substrates%2C%20the%20biophysical%20properties%20of%20these%20organelles%20allow%20novel%20PQC%20functions.%20In%20this%20review%2C%20we%20explore%20the%20specificity%20of%20the%20nuclear%20compartment%2C%20the%20effectors%20of%20the%20nuclear%20proteostasis%20network%2C%20and%20the%20PQC%20roles%20of%20nuclear%20membrane-less%20organelles%20in%20metazoans.%22%2C%22date%22%3A%222024-04-18%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.biochi.2024.04.006%22%2C%22ISSN%22%3A%221638-6183%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-22T06%3A21%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22T29V8YIU%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Farhadova%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFarhadova%2C%20Sabina%2C%20Amani%20Ghousein%2C%20Fran%26%23xE7%3Bois%20Charon%2C%20Caroline%20Surcis%2C%20Melisa%20Gomez-Velazques%2C%20Clara%20Roidor%2C%20Flavio%20Di%20Michele%2C%20et%20al.%202024.%20%26%23x201C%3BThe%20Long%20Non-Coding%20RNA%20Meg3%20Mediates%20Imprinted%20Gene%20Expression%20during%20Stem%20Cell%20Differentiation.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2C%20April%2C%20gkae247.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae247%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae247%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20long%20non-coding%20RNA%20Meg3%20mediates%20imprinted%20gene%20expression%20during%20stem%20cell%20differentiation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabina%22%2C%22lastName%22%3A%22Farhadova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amani%22%2C%22lastName%22%3A%22Ghousein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois%22%2C%22lastName%22%3A%22Charon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Surcis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Melisa%22%2C%22lastName%22%3A%22Gomez-Velazques%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Clara%22%2C%22lastName%22%3A%22Roidor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Flavio%22%2C%22lastName%22%3A%22Di%20Michele%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maud%22%2C%22lastName%22%3A%22Borensztein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Albertina%22%2C%22lastName%22%3A%22De%20Sario%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cyril%22%2C%22lastName%22%3A%22Esnault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daan%22%2C%22lastName%22%3A%22Noordermeer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Moindrot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Feil%22%7D%5D%2C%22abstractNote%22%3A%22The%20imprinted%20Dlk1-Dio3%20domain%20comprises%20the%20developmental%20genes%20Dlk1%20and%20Rtl1%2C%20which%20are%20silenced%20on%20the%20maternal%20chromosome%20in%20different%20cell%20types.%20On%20this%20parental%20chromosome%2C%20the%20domain%27s%20imprinting%20control%20region%20activates%20a%20polycistron%20that%20produces%20the%20lncRNA%20Meg3%20and%20many%20miRNAs%20%28Mirg%29%20and%20C%5C%2FD-box%20snoRNAs%20%28Rian%29.%20Although%20Meg3%20lncRNA%20is%20nuclear%20and%20associates%20with%20the%20maternal%20chromosome%2C%20it%20is%20unknown%20whether%20it%20controls%20gene%20repression%20in%20cis.%20We%20created%20mouse%20embryonic%20stem%20cells%20%28mESCs%29%20that%20carry%20an%20ectopic%20poly%28A%29%20signal%2C%20reducing%20RNA%20levels%20along%20the%20polycistron%2C%20and%20generated%20Rian-%5C%2F-%20mESCs%20as%20well.%20Upon%20ESC%20differentiation%2C%20we%20found%20that%20Meg3%20lncRNA%20%28but%20not%20Rian%29%20is%20required%20for%20Dlk1%20repression%20on%20the%20maternal%20chromosome.%20Biallelic%20Meg3%20expression%20acquired%20through%20CRISPR-mediated%20demethylation%20of%20the%20paternal%20Meg3%20promoter%20led%20to%20biallelic%20Dlk1%20repression%2C%20and%20to%20loss%20of%20Rtl1%20expression.%20lncRNA%20expression%20also%20correlated%20with%20DNA%20hypomethylation%20and%20CTCF%20binding%20at%20the%205%27-side%20of%20Meg3.%20Using%20Capture%20Hi-C%2C%20we%20found%20that%20this%20creates%20a%20Topologically%20Associating%20Domain%20%28TAD%29%20organization%20that%20brings%20Meg3%20close%20to%20Dlk1%20on%20the%20maternal%20chromosome.%20The%20requirement%20of%20Meg3%20for%20gene%20repression%20and%20TAD%20structure%20may%20explain%20how%20aberrant%20MEG3%20expression%20at%20the%20human%20DLK1-DIO3%20locus%20associates%20with%20imprinting%20disorders.%22%2C%22date%22%3A%222024-04-13%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkae247%22%2C%22ISSN%22%3A%221362-4962%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-15T06%3A56%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22WF4SDE8E%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Trometer%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETrometer%2C%20Nathan%2C%20J%26%23xE9%3Br%26%23xE9%3Bmy%20Pecourneau%2C%20Liwen%20Feng%2C%20Jos%26%23xE9%3B%20A.%20Navarro-Huerta%2C%20Danielle%20Lazarin-Bid%26%23xF3%3Bia%2C%20Sueli%20de%20Oliveira%20Silva%20Lautenschlager%2C%20Louis%20Maes%2C%20et%20al.%202024.%20%26%23x201C%3BSynthesis%20and%20Anti-Chagas%20Activity%20Profile%20of%20a%20Redox-Active%20Lead%203-Benzylmenadione%20Revealed%20by%20High-Content%20Imaging.%26%23x201D%3B%20%3Ci%3EACS%20Infectious%20Diseases%3C%5C%2Fi%3E%2C%20April.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facsinfecdis.4c00137%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facsinfecdis.4c00137%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Synthesis%20and%20Anti-Chagas%20Activity%20Profile%20of%20a%20Redox-Active%20Lead%203-Benzylmenadione%20Revealed%20by%20High-Content%20Imaging%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathan%22%2C%22lastName%22%3A%22Trometer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00e9my%22%2C%22lastName%22%3A%22Pecourneau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Liwen%22%2C%22lastName%22%3A%22Feng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jos%5Cu00e9%20A.%22%2C%22lastName%22%3A%22Navarro-Huerta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Danielle%22%2C%22lastName%22%3A%22Lazarin-Bid%5Cu00f3ia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sueli%22%2C%22lastName%22%3A%22de%20Oliveira%20Silva%20Lautenschlager%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Louis%22%2C%22lastName%22%3A%22Maes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amanda%22%2C%22lastName%22%3A%22Fortes%20Francisco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20M.%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brigitte%22%2C%22lastName%22%3A%22Meunier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Monica%22%2C%22lastName%22%3A%22Cal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22M%5Cu00e4ser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marcel%22%2C%22lastName%22%3A%22Kaiser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisabeth%22%2C%22lastName%22%3A%22Davioud-Charvet%22%7D%5D%2C%22abstractNote%22%3A%22Chagas%20disease%2C%20or%20American%20trypanosomiasis%2C%20is%20a%20neglected%20tropical%20disease%20which%20is%20a%20top%20priority%20target%20of%20the%20World%20Health%20Organization.%20The%20disease%2C%20endemic%20mainly%20in%20Latin%20America%2C%20is%20caused%20by%20the%20protozoan%20Trypanosoma%20cruzi%20and%20has%20spread%20around%20the%20globe%20due%20to%20human%20migration.%20There%20are%20multiple%20transmission%20routes%2C%20including%20vectorial%2C%20congenital%2C%20oral%2C%20and%20iatrogenic.%20Less%20than%201%25%20of%20patients%20have%20access%20to%20treatment%2C%20relying%20on%20two%20old%20redox-active%20drugs%20that%20show%20poor%20pharmacokinetics%20and%20severe%20adverse%20effects.%20Hence%2C%20the%20priorities%20for%20the%20next%20steps%20of%20R%26D%20include%20%28i%29%20the%20discovery%20of%20novel%20drugs%5C%2Fchemical%20classes%2C%20%28ii%29%20filling%20the%20pipeline%20with%20drug%20candidates%20that%20have%20new%20mechanisms%20of%20action%2C%20and%20%28iii%29%20the%20pressing%20need%20for%20more%20research%20and%20access%20to%20new%20chemical%20entities.%20In%20the%20present%20work%2C%20we%20first%20identified%20a%20hit%20%284a%29%20with%20a%20potent%20anti-T.%20cruzi%20activity%20from%20a%20library%20of%203-benzylmenadiones.%20We%20then%20designed%20a%20synthetic%20strategy%20to%20build%20a%20library%20of%2049%203-%284-monoamino%29benzylmenadione%20derivatives%20via%20reductive%20amination%20to%20obtain%20diazacyclic%20benz%28o%29ylmenadiones.%20Among%20them%2C%20we%20identified%20by%20high%20content%20imaging%20an%20anti-amastigote%20%5C%22early%20lead%5C%22%2011b%20%28henceforth%20called%20cruzidione%29%20revealing%20optimized%20pharmacokinetic%20properties%20and%20enhanced%20specificity.%20Studies%20in%20a%20yeast%20model%20revealed%20that%20a%20cruzidione%20metabolite%2C%20the%203-benzoylmenadione%20%28cruzidione%20oxide%29%2C%20enters%20redox%20cycling%20with%20the%20NADH-dehydrogenase%2C%20generating%20reactive%20oxygen%20species%2C%20as%20hypothesized%20for%20the%20early%20hit%20%284a%29.%22%2C%22date%22%3A%222024-04-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1021%5C%2Facsinfecdis.4c00137%22%2C%22ISSN%22%3A%222373-8227%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-15T06%3A57%3A43Z%22%7D%7D%2C%7B%22key%22%3A%22VMMVM6YY%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Saunier%20et%20al.%22%2C%22parsedDate%22%3A%222024-04-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESaunier%2C%20Marion%2C%20Louis-Charles%20Fortier%2C%20and%20Olga%20Soutourina.%202024.%20%26%23x201C%3BRNA-Based%20Regulation%20in%20Bacteria-Phage%20Interactions.%26%23x201D%3B%20%3Ci%3EAnaerobe%3C%5C%2Fi%3E%2C%20April%2C%20102851.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.anaerobe.2024.102851%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.anaerobe.2024.102851%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22RNA-based%20regulation%20in%20bacteria-phage%20interactions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Saunier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Louis-Charles%22%2C%22lastName%22%3A%22Fortier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%22%2C%22lastName%22%3A%22Soutourina%22%7D%5D%2C%22abstractNote%22%3A%22Interactions%20of%20bacteria%20with%20their%20viruses%20named%20bacteriophages%20or%20phages%20shape%20the%20bacterial%20genome%20evolution%20and%20contribute%20to%20the%20diversity%20of%20phages.%20RNAs%20have%20emerged%20as%20key%20components%20of%20several%20anti-phage%20defense%20systems%20in%20bacteria%20including%20CRISPR-Cas%2C%20toxin-antitoxin%20and%20abortive%20infection.%20Frequent%20association%20with%20mobile%20genetic%20elements%20and%20interplay%20between%20different%20anti-phage%20defense%20systems%20are%20largely%20discussed.%20Newly%20discovered%20defense%20systems%20such%20as%20retrons%20and%20CBASS%20include%20RNA%20components.%20RNAs%20also%20perform%20their%20well-recognized%20regulatory%20roles%20in%20crossroad%20of%20phage-bacteria%20regulatory%20networks.%20Both%20regulatory%20and%20defensive%20function%20can%20be%20sometimes%20attributed%20to%20the%20same%20RNA%20molecules%20including%20CRISPR%20RNAs.%20This%20review%20presents%20the%20recent%20advances%20on%20the%20role%20of%20RNAs%20in%20the%20bacteria-phage%20interactions%20with%20a%20particular%20focus%20on%20clostridial%20species%20including%20an%20important%20human%20pathogen%20Clostridioides%20difficile.%22%2C%22date%22%3A%222024-04-05%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.anaerobe.2024.102851%22%2C%22ISSN%22%3A%221095-8274%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-08T08%3A21%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22HFFNKR9R%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Figueroa-Bossi%20et%20al.%22%2C%22parsedDate%22%3A%222024-03-30%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFigueroa-Bossi%2C%20Nara%2C%20Roc%26%23xED%3Bo%20Fern%26%23xE1%3Bndez-Fern%26%23xE1%3Bndez%2C%20Patricia%20Kerboriou%2C%20Philippe%20Bouloc%2C%20Josep%20Casades%26%23xFA%3Bs%2C%20Mar%26%23xED%3Ba%20Antonia%20S%26%23xE1%3Bnchez-Romero%2C%20and%20Lionello%20Bossi.%202024.%20%26%23x201C%3BTranscription-Driven%20DNA%20Supercoiling%20Counteracts%20H-NS-Mediated%20Gene%20Silencing%20in%20Bacterial%20Chromatin.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2015%20%281%29%3A%202787.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-024-47114-w%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-024-47114-w%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Transcription-driven%20DNA%20supercoiling%20counteracts%20H-NS-mediated%20gene%20silencing%20in%20bacterial%20chromatin%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nara%22%2C%22lastName%22%3A%22Figueroa-Bossi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roc%5Cu00edo%22%2C%22lastName%22%3A%22Fern%5Cu00e1ndez-Fern%5Cu00e1ndez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Kerboriou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Bouloc%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Josep%22%2C%22lastName%22%3A%22Casades%5Cu00fas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mar%5Cu00eda%20Antonia%22%2C%22lastName%22%3A%22S%5Cu00e1nchez-Romero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lionello%22%2C%22lastName%22%3A%22Bossi%22%7D%5D%2C%22abstractNote%22%3A%22In%20all%20living%20cells%2C%20genomic%20DNA%20is%20compacted%20through%20interactions%20with%20dedicated%20proteins%20and%5C%2For%20the%20formation%20of%20plectonemic%20coils.%20In%20bacteria%2C%20DNA%20compaction%20is%20achieved%20dynamically%2C%20coordinated%20with%20dense%20and%20constantly%20changing%20transcriptional%20activity.%20H-NS%2C%20a%20major%20bacterial%20nucleoid%20structuring%20protein%2C%20is%20of%20special%20interest%20due%20to%20its%20interplay%20with%20RNA%20polymerase.%20H-NS%3ADNA%20nucleoprotein%20filaments%20inhibit%20transcription%20initiation%20by%20RNA%20polymerase.%20However%2C%20the%20discovery%20that%20genes%20silenced%20by%20H-NS%20can%20be%20activated%20by%20transcription%20originating%20from%20neighboring%20regions%20has%20suggested%20that%20elongating%20RNA%20polymerases%20can%20disassemble%20H-NS%3ADNA%20filaments.%20In%20this%20study%2C%20we%20present%20evidence%20that%20transcription-induced%20counter-silencing%20does%20not%20require%20transcription%20to%20reach%20the%20silenced%20gene%3B%20rather%2C%20it%20exerts%20its%20effect%20at%20a%20distance.%20Counter-silencing%20is%20suppressed%20by%20introducing%20a%20DNA%20gyrase%20binding%20site%20within%20the%20intervening%20segment%2C%20suggesting%20that%20the%20long-range%20effect%20results%20from%20transcription-driven%20positive%20DNA%20supercoils%20diffusing%20toward%20the%20silenced%20gene.%20We%20propose%20a%20model%20wherein%20H-NS%3ADNA%20complexes%20form%20in%20vivo%20on%20negatively%20supercoiled%20DNA%2C%20with%20H-NS%20bridging%20the%20two%20arms%20of%20the%20plectoneme.%20Rotational%20diffusion%20of%20positive%20supercoils%20generated%20by%20neighboring%20transcription%20will%20cause%20the%20H-NS-bound%20negatively-supercoiled%20plectoneme%20to%20%5C%22unroll%5C%22%20disrupting%20the%20H-NS%20bridges%20and%20releasing%20H-NS.%22%2C%22date%22%3A%222024-03-30%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-024-47114-w%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-02T12%3A03%3A56Z%22%7D%7D%2C%7B%22key%22%3A%22IZMW7M25%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Vincent%20et%20al.%22%2C%22parsedDate%22%3A%222024-03-29%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVincent%2C%20Marine%2C%20Victoire%20Blanc-Garin%2C%20C%26%23xE9%3Blia%20Chenebault%2C%20Mattia%20Cirimele%2C%20Sandrine%20Farci%2C%20Luis%20Fernando%20Garcia-Alles%2C%20Corinne%20Cassier-Chauvat%2C%20and%20Franck%20Chauvat.%202024.%20%26%23x201C%3BImpact%20of%20Carbon%20Fixation%2C%20Distribution%20and%20Storage%20on%20the%20Production%20of%20Farnesene%20and%20Limonene%20in%20Synechocystis%20PCC%206803%20and%20Synechococcus%20PCC%207002.%26%23x201D%3B%20%3Ci%3EInternational%20Journal%20of%20Molecular%20Sciences%3C%5C%2Fi%3E%2025%20%287%29%3A%203827.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms25073827%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms25073827%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Impact%20of%20Carbon%20Fixation%2C%20Distribution%20and%20Storage%20on%20the%20Production%20of%20Farnesene%20and%20Limonene%20in%20Synechocystis%20PCC%206803%20and%20Synechococcus%20PCC%207002%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marine%22%2C%22lastName%22%3A%22Vincent%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Victoire%22%2C%22lastName%22%3A%22Blanc-Garin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9lia%22%2C%22lastName%22%3A%22Chenebault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mattia%22%2C%22lastName%22%3A%22Cirimele%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sandrine%22%2C%22lastName%22%3A%22Farci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luis%20Fernando%22%2C%22lastName%22%3A%22Garcia-Alles%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Corinne%22%2C%22lastName%22%3A%22Cassier-Chauvat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Franck%22%2C%22lastName%22%3A%22Chauvat%22%7D%5D%2C%22abstractNote%22%3A%22Terpenes%20are%20high-value%20chemicals%20which%20can%20be%20produced%20by%20engineered%20cyanobacteria%20from%20sustainable%20resources%2C%20solar%20energy%2C%20water%20and%20CO2.%20We%20previously%20reported%20that%20the%20euryhaline%20unicellular%20cyanobacteria%20Synechocystis%20sp.%20PCC%206803%20%28S.6803%29%20and%20Synechococcus%20sp.%20PCC%207002%20%28S.7002%29%20produce%20farnesene%20and%20limonene%2C%20respectively%2C%20more%20efficiently%20than%20other%20terpenes.%20In%20the%20present%20study%2C%20we%20attempted%20to%20enhance%20farnesene%20production%20in%20S.6803%20and%20limonene%20production%20in%20S.7002.%20Practically%2C%20we%20tested%20the%20influence%20of%20key%20cyanobacterial%20enzymes%20acting%20in%20carbon%20fixation%20%28RubisCO%2C%20PRK%2C%20CcmK3%20and%20CcmK4%29%2C%20utilization%20%28CrtE%2C%20CrtR%20and%20CruF%29%20and%20storage%20%28PhaA%20and%20PhaB%29%20on%20terpene%20production%20in%20S.6803%2C%20and%20we%20compared%20some%20of%20the%20findings%20with%20the%20data%20obtained%20in%20S.7002.%20We%20report%20that%20the%20overproduction%20of%20RubisCO%20from%20S.7002%20and%20PRK%20from%20Cyanothece%20sp.%20PCC%207425%20increased%20farnesene%20production%20in%20S.6803%2C%20but%20not%20limonene%20production%20in%20S.7002.%20The%20overexpression%20of%20the%20crtE%20genes%20%28synthesis%20of%20terpene%20precursors%29%20from%20S.6803%20or%20S.7002%20did%20not%20increase%20farnesene%20production%20in%20S.6803.%20In%20contrast%2C%20the%20overexpression%20of%20the%20crtE%20gene%20from%20S.6803%2C%20but%20not%20S.7002%2C%20increased%20farnesene%20production%20in%20S.7002%2C%20emphasizing%20the%20physiological%20difference%20between%20these%20two%20model%20cyanobacteria.%20Furthermore%2C%20the%20deletion%20of%20the%20crtR%20and%20cruF%20genes%20%28carotenoid%20synthesis%29%20and%20phaAB%20genes%20%28carbon%20storage%29%20did%20not%20increase%20the%20production%20of%20farnesene%20in%20S.6803.%20Finally%2C%20as%20a%20containment%20strategy%20of%20genetically%20modified%20strains%20of%20S.6803%2C%20we%20report%20that%20the%20deletion%20of%20the%20ccmK3K4%20genes%20%28carboxysome%20for%20CO2%20fixation%29%20did%20not%20affect%20the%20production%20of%20limonene%2C%20but%20decreased%20the%20production%20of%20farnesene%20in%20S.6803.%22%2C%22date%22%3A%222024-03-29%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms25073827%22%2C%22ISSN%22%3A%221422-0067%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-04-15T06%3A57%3A08Z%22%7D%7D%5D%7D
Salama, Ehab A., Yehia Elgammal, Aruna Wijeratne, Nadia A. Lanman, Sagar M. Utturkar, Atena Farhangian, Jianing Li, Brigitte Meunier, Tony R. Hazbun, and Mohamed N. Seleem. 2024. “Lansoprazole Interferes with Fungal Respiration and Acts Synergistically with Amphotericin B against Multidrug-Resistant Candida Auris.” Emerging Microbes & Infections 13 (1): 2322649. https://doi.org/10.1080/22221751.2024.2322649.
Catalina-Hernández, Èric, Mario López-Martín, David Masnou-Sánchez, Marco Martins, Victor A. Lorenz-Fonfria, Francesc Jiménez-Altayó, Ute A. Hellmich, et al. 2024. “Experimental and Computational Biophysics to Identify Vasodilator Drugs Targeted at TRPV2 Using Agonists Based on the Probenecid Scaffold.” Computational and Structural Biotechnology Journal 23 (December): 473–82. https://doi.org/10.1016/j.csbj.2023.12.028.
Oumerri, Jihad, Hicham Qayouh, Ana Andreea Arteni, Jean Luc Six, Mohammed Lahcini, and Khalid Ferji. 2024. “One-Pot Formulation of Cationic Oligochitosan Coated Nanoparticles via Photo- Polymerization Induced Self-Assembly.” Chemphyschem: A European Journal of Chemical Physics and Physical Chemistry, April, e202400291. https://doi.org/10.1002/cphc.202400291.
Iacobucci, Ilaria, Vittoria Monaco, Agnès Hovasse, Baptiste Dupouy, Rodrigue Keumoe, Bogdan Cichocki, Mourad Elhabiri, et al. 2024. “Proteomic Profiling of Antimalarial Plasmodione Using 3-Benz(o)Ylmenadione Affinity-Based Probes.” Chembiochem: A European Journal of Chemical Biology, April, e202400187. https://doi.org/10.1002/cbic.202400187.
Buggiani, Julia, Thierry Meinnel, Carmela Giglione, and Frédéric Frottin. 2024. “Advances in Nuclear Proteostasis of Metazoans.” Biochimie, April, S0300-9084(24)00081-6. https://doi.org/10.1016/j.biochi.2024.04.006.
Farhadova, Sabina, Amani Ghousein, François Charon, Caroline Surcis, Melisa Gomez-Velazques, Clara Roidor, Flavio Di Michele, et al. 2024. “The Long Non-Coding RNA Meg3 Mediates Imprinted Gene Expression during Stem Cell Differentiation.” Nucleic Acids Research, April, gkae247. https://doi.org/10.1093/nar/gkae247.
Trometer, Nathan, Jérémy Pecourneau, Liwen Feng, José A. Navarro-Huerta, Danielle Lazarin-Bidóia, Sueli de Oliveira Silva Lautenschlager, Louis Maes, et al. 2024. “Synthesis and Anti-Chagas Activity Profile of a Redox-Active Lead 3-Benzylmenadione Revealed by High-Content Imaging.” ACS Infectious Diseases, April. https://doi.org/10.1021/acsinfecdis.4c00137.
Saunier, Marion, Louis-Charles Fortier, and Olga Soutourina. 2024. “RNA-Based Regulation in Bacteria-Phage Interactions.” Anaerobe, April, 102851. https://doi.org/10.1016/j.anaerobe.2024.102851.
Figueroa-Bossi, Nara, Rocío Fernández-Fernández, Patricia Kerboriou, Philippe Bouloc, Josep Casadesús, María Antonia Sánchez-Romero, and Lionello Bossi. 2024. “Transcription-Driven DNA Supercoiling Counteracts H-NS-Mediated Gene Silencing in Bacterial Chromatin.” Nature Communications 15 (1): 2787. https://doi.org/10.1038/s41467-024-47114-w.
Vincent, Marine, Victoire Blanc-Garin, Célia Chenebault, Mattia Cirimele, Sandrine Farci, Luis Fernando Garcia-Alles, Corinne Cassier-Chauvat, and Franck Chauvat. 2024. “Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002.” International Journal of Molecular Sciences 25 (7): 3827. https://doi.org/10.3390/ijms25073827.