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Julien, Manon, Sophie Zinn-Justin, and Francois-Xavier Theillet. 2026. “Advanced NMR Characterization and Sensitive Detection of Isoaspartate in Proteins.” Analytical Chemistry, ahead of print, April 21. https://doi.org/10.1021/acs.analchem.5c07290.
Arnaiz, Olivier, Frédéric Guérin, Arnaud Couloux, et al. 2026. “The Tiny Germline Chromosomes of Paramecium Aurelia Have an Exceptionally High Recombination Rate and Are Capped by a New Class of Helitrons.” BMC Biology 24 (1): 99. https://doi.org/10.1186/s12915-026-02584-w.
Malesinski, Soazig, André Vidal-Meireles, Eve Giovannetti, et al. 2026. “Acclimation of Photosynthesis to the Environment 1 Acts as a Copper-Binding Superoxide Detoxification Enzyme.” Nature Communications, ahead of print, April 15. https://doi.org/10.1038/s41467-026-71893-z.
Balan, Thomas, Mélanie Bazin-Gélis, Marc Guérineau, et al. 2026. “A Developmental Condensin I Complex Assists the Paramecium PiggyMac Domesticated Transposase during Programmed DNA Elimination.” Nucleic Acids Research 54 (7): gkag351. https://doi.org/10.1093/nar/gkag351.
Agnoli, Kirsty, Anugraha Mathew, Stefano Gualdi, et al. 2026. “Identifying Phenotypic and Genetic Traits for Assessing Pathogenic Potential and Biocontrol Capacity in Burkholderia Sensu Lato Strains.” The ISME Journal, April 10, wrag081. https://doi.org/10.1093/ismejo/wrag081.
Gamet, Antoine, Ismaila Ciss, Arthur Courtois, et al. 2026. “Total Synthesis of Photoswitchable Latrunculin B Enables Reversible Control of Actin Polymerization and Cell Migration.” Journal of the American Chemical Society, ahead of print, April 8. https://doi.org/10.1021/jacs.5c17295.
Corroyer-Dulmont, Simon, Audrey Labarde, Vojtěch Pražák, et al. 2026. “Subcellular Reorganization upon Phage Infection Reveals Stepwise Assembly of Viral Particles from Membrane-Associated Precursors.” Nature Communications, ahead of print, April 2. https://doi.org/10.1038/s41467-026-71181-w.
Jeanne, Etienne, Marta Torres, Anthony Quéro, Delphine Naquin, and Denis Faure. 2026. “Carbon Metabolic Versatility of Agrobacterium Tumefaciens in Tomato Roots and Galls.” Environmental Microbiology 28 (4): e70311. https://doi.org/10.1111/1462-2920.70311.
Affholder, Julien, Louis Lambret, Sara Penaranda, et al. 2026. “Strigolactones and Sesquiterpene Lactones Induce Orobanche Cumana Germination via KAI2d Receptors through Distinct Processes.” The Plant Journal: For Cell and Molecular Biology 126 (1): e70852. https://doi.org/10.1111/tpj.70852.
Khamvongsa-Charbonnier, Lucie, Robert Aboukhalil, Hélène Chiapello, et al. 2026. “Training Biologists in Unix Command-Line Skills: From Curriculum to Interactive Online Tutorials.” PLoS Computational Biology 22 (4): e1014133. https://doi.org/10.1371/journal.pcbi.1014133.