I2BC publishes 150
articles and reviews a year
Latest Publications
3888256
i2bc
1
chicago-author-date
10
date
desc
year
4613
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22TJH5YWVE%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Piet%20et%20al.%22%2C%22parsedDate%22%3A%222026-05-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BPiet%2C%20Quentin%2C%20Pascale%20Besse%2C%20St%26%23xE9%3Bphanie%20Bocs%2C%20et%20al.%202026.%20%26%23x201C%3BA%20Chromosome-Level%20Genome%20Assembly%20of%20Vanilla%20Planifolia%20Uncovers%20the%20Genomic%20Architecture%20Underlying%20Partial%20Endoreplication.%26%23x201D%3B%20%26lt%3Bi%26gt%3BBMC%20Genomics%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20May%2013.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12864-026-12926-1%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12864-026-12926-1%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20chromosome-level%20genome%20assembly%20of%20Vanilla%20planifolia%20uncovers%20the%20genomic%20architecture%20underlying%20partial%20endoreplication%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Quentin%22%2C%22lastName%22%3A%22Piet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascale%22%2C%22lastName%22%3A%22Besse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phanie%22%2C%22lastName%22%3A%22Bocs%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Bouchez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mickael%22%2C%22lastName%22%3A%22Bourge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carine%22%2C%22lastName%22%3A%22Charron%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Cravero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amaury%22%2C%22lastName%22%3A%22Dedours%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ga%5Cu00ebtan%22%2C%22lastName%22%3A%22Droc%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michel%22%2C%22lastName%22%3A%22Dron%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michel%22%2C%22lastName%22%3A%22Grisoni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Klopp%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fanny%22%2C%22lastName%22%3A%22Lambert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%22%2C%22lastName%22%3A%22Marande%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hadrien%20Mat%5Cu00e9%22%2C%22lastName%22%3A%22de%20G%5Cu00e9rando%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathalie%22%2C%22lastName%22%3A%22Rodde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marine%22%2C%22lastName%22%3A%22Sallaberry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonja%22%2C%22lastName%22%3A%22Siljak-Yakovlev%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marilyne%22%2C%22lastName%22%3A%22Summo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cyril%22%2C%22lastName%22%3A%22Jourda%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Partial%20endoreplication%20is%20a%20prominent%20developmental%20feature%20and%20poses%20a%20significant%20challenge%20for%20whole%20genome%20assembly%20in%20orchids.%20This%20form%20of%20cell%20cycle%20results%20in%20highly%20unbalanced%20cell%20DNA%20content%2C%20with%20the%20highly%20endoreplicated%20%28P%29%20fraction%20being%20overrepresented%20in%20sequencing%20data%20compared%20to%20the%20non-endoreplicated%20%28F%29%20fraction.%5CnRESULTS%3A%20Here%2C%20we%20report%20the%20first%20genome%20assembly%20of%20Vanilla%20planifolia%20into%2016%20chromosome%20pairs%20using%20axillary%20buds%20enriched%20in%20non-endoreplicated%202%5Cu00a0C-nuclei%20%2855%25%29%20as%20determined%20by%20flow%20cytometry.%20The%20assembly%20was%20generated%20using%20a%20hybrid%20approach%20combining%20PacBio%20HiFi%20sequencing%20and%20Omni-C%20scaffolding%20generated%20in%20this%20study%2C%20together%20with%20a%20GBS-SNP%20genetic%20map%20and%20Oxford%20Nanopore%20Technologies%20long-read%20data%20from%20the%20literature.%20For%20the%20first%20time%2C%20we%20identified%20P%20and%20F%20regions%20within%20reconstructed%20chromosomes%2C%20representing%2020.57%25%20and%2079.43%25%20of%20the%20genome%2C%20respectively%20based%20on%20DNA%20sequencing%20data%20from%20three%20tissues%20with%20varying%20levels%20of%20endoreplicated%20nuclei.%20P%20regions%20were%20gene-rich%20and%20located%20at%20chromosome%20ends%2C%20whereas%20F%20regions%20were%20SSR-rich%20and%20located%20at%20central%20parts%20of%20chromosomes.%20Remarkably%2C%2097.24%25%20of%20SSRs%20were%20found%20in%20F%20regions%2C%20predominantly%20comprising%20the%20trinucleotide%20AAG%5C%2FCTT%20motif%2C%20which%20may%20contribute%20to%20the%20absence%20of%20endoreplication%20in%20these%20regions.%20Protein-encoding%20genes%20overrepresented%20in%20F%20regions%20were%20associated%20with%20negative%20regulation%20of%20flower%20development%2C%20mitotic%20cycle%20progression%2C%20cell%20division%20and%20histone%20modification.%5CnCONCLUSIONS%3A%20This%20accurate%20high-quality%20chromosome-scale%20V.%20planifolia%20genome%20assembly%20provides%20unprecedented%20insights%20into%20the%20structural%20and%20molecular%20characteristics%20of%20partial%20endoreplication%20in%20Orchids%20and%20represents%20a%20major%20step%20toward%20the%20characterization%20of%20this%20complex%20genome.%22%2C%22date%22%3A%222026-05-13%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12864-026-12926-1%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242121026%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221471-2164%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A40%3A12Z%22%7D%7D%2C%7B%22key%22%3A%22FRFAJYL5%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zahid%20et%20al.%22%2C%22parsedDate%22%3A%222026-05-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BZahid%2C%20Sayma%2C%20Jeanne%20Chauvat%2C%20Ilaria%20Ceppi%2C%20et%20al.%202026.%20%26%23x201C%3BStructural%20Basis%20of%20Ku-Mediated%20Activation%20of%20WRN%20Exonuclease%20Activity.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNature%20Communications%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20May%2013.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-026-71888-w%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-026-71888-w%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20basis%20of%20Ku-mediated%20activation%20of%20WRN%20exonuclease%20activity%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sayma%22%2C%22lastName%22%3A%22Zahid%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeanne%22%2C%22lastName%22%3A%22Chauvat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ilaria%22%2C%22lastName%22%3A%22Ceppi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Floriana%22%2C%22lastName%22%3A%22Cappiello%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benedetta%22%2C%22lastName%22%3A%22Perdichizzi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Frit%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dennis%22%2C%22lastName%22%3A%22Gomez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steven%20W.%22%2C%22lastName%22%3A%22Hardwick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Legrand%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22Karazi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonia%22%2C%22lastName%22%3A%22Baconnais%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22G%5Cu00e9rard%22%2C%22lastName%22%3A%22Pehau-Arnaudet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S%5Cu00e9bastien%22%2C%22lastName%22%3A%22Britton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Baptiste%22%2C%22lastName%22%3A%22Charbonnier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amanda%20K.%22%2C%22lastName%22%3A%22Chaplin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pietro%22%2C%22lastName%22%3A%22Pichierri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Cejka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Calsou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginie%22%2C%22lastName%22%3A%22Ropars%22%7D%5D%2C%22abstractNote%22%3A%22Werner%20%28WRN%29%20is%20the%20only%20human%20RecQ%20helicase%20family%20member%20with%20DNA%20exonuclease%20activity.%20WRN%20promotes%20genome%20stability%20through%20its%20functions%20in%20DNA%20replication%2C%20repair%20and%20telomere%20maintenance%2C%20the%20deficiency%20of%20which%20presents%20clinically%20as%20Werner%20syndrome%2C%20causing%20premature%20aging%20and%20cancer%20predisposition.%20The%20main%20DNA%20double%20strand-break%20sensor%20Ku70%5C%2F80%20heterodimer%20%28Ku%29%20is%20a%20known%20partner%20of%20WRN%2C%20which%20stimulates%20its%20nuclease%20activity.%20However%2C%20the%20molecular%20basis%20of%20Ku-WRN%20interplay%20is%20currently%20unknown.%20Here%2C%20we%20present%20a%20high%20resolution%20cryo-EM%20structure%20of%20human%20Ku%20bound%20to%20DNA%20in%20complex%20with%20the%20N-terminal%20WRN%20exonuclease%20domain.%20This%20structure%20reveals%20multiple%20interaction%20sites%20between%20WRN%20and%20the%20Ku%3ADNA%20complex.%20The%20catalytic%20domain%20of%20WRN-exo%20engages%20with%20the%20DNA%20ends%2C%20stabilized%20by%20the%20vWA-like%20Ku80%20domain%20interacting%20with%20the%20N-terminal%20APLF-like%20Ku%20binding%20motif%20%28A-KBM%29%20of%20WRN.%20Most%20surprisingly%2C%20we%20visualize%20the%20SAP%20domain%20of%20Ku70%20stabilized%20within%20this%20complex%2C%20and%20we%20identify%20specific%20contacts%20mediating%20this%20interaction.%20These%20interactions%20are%20validated%20by%20assessing%20the%20impact%20of%20point%20mutations%20on%20either%20side%20of%20the%20Ku-WRN%20interfaces%20on%20exonuclease%20activity%20with%20purified%20recombinant%20proteins%2C%20and%20on%20live%20protein%20recruitment%20at%20biphoton%20laser-damaged%20nuclear%20sites.%20Finally%2C%20we%20show%20that%20disruption%20of%20WRN-Ku70%20interaction%20results%20in%20aberrant%20resection%20of%20stalled%20replication%20forks.%20Together%2C%20we%20define%20the%20architecture%20of%20the%20Ku-WRN%20exonuclease%20domain%20interface%20and%20its%20impact%20on%20WRN%20exonuclease%20activity%2C%20recruitment%20and%20replication%20fork%20processing.%22%2C%22date%22%3A%222026-05-13%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-026-71888-w%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242129166%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222041-1723%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A36%3A42Z%22%7D%7D%2C%7B%22key%22%3A%22STLP2UBX%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Guerringue%20et%20al.%22%2C%22parsedDate%22%3A%222026-05-07%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BGuerringue%2C%20Yannick%2C%20Sebastien%20Thomine%2C%20Jean-Marc%20Allain%2C%20and%20Jean-Marie%20Frachisse.%202026.%20%26%23x201C%3BThe%20Rapid%20Mechanically%20Activated%20Channel%20Transduces%20Increases%20in%20Plasma%20Membrane%20Tension%20into%20Transient%20Calcium%20Influx.%26%23x201D%3B%20%26lt%3Bi%26gt%3BThe%20New%20Phytologist%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20May%207.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.71241%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.71241%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Rapid%20Mechanically%20Activated%20channel%20transduces%20increases%20in%20plasma%20membrane%20tension%20into%20transient%20calcium%20influx%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yannick%22%2C%22lastName%22%3A%22Guerringue%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sebastien%22%2C%22lastName%22%3A%22Thomine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Marc%22%2C%22lastName%22%3A%22Allain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Marie%22%2C%22lastName%22%3A%22Frachisse%22%7D%5D%2C%22abstractNote%22%3A%22Plants%20respond%20to%20mechanical%20stimuli%20by%20a%20rapid%20increase%20in%20cytosolic%20calcium.%20The%20intensity%20and%20kinetics%20of%20the%20calcium%20changes%20define%20calcium%20signatures%20important%20for%20biological%20responses.%20In%20this%20study%2C%20we%20determine%20the%20properties%20of%20a%20calcium-permeable%20force-gated%20channel%20localized%20at%20the%20plasma%20membrane%20called%20Rapid%20Mechanically%20Activated%20%28RMA%29.%20Using%20patch%20clamp%20and%20pressure%20clamp%2C%20we%20characterized%20the%20kinetics%20of%20the%20Arabidopsis%20thaliana%20RMA%20channel%20upon%20stimulation%20by%20pressure%20pulses%20applied%20onto%20the%20plasma%20membrane.%20Combining%20pressure%20pulse%20protocols%20at%20different%20frequencies%20with%20modeling%2C%20we%20investigated%20the%20channel%26%23039%3Bs%20capacity%20to%20transduce%20high%20frequency%20mechanical%20stimuli.%20The%20RMA%20channel%20rapidly%20activates%20in%20response%20to%20membrane%20tension%2C%20then%20it%20inactivates%20during%20prolonged%20stimulation.%20Upon%20repeated%20stimulations%2C%20the%20RMA%20current%20amplitude%20decreases%20irreversibly%20indicating%20that%20it%20undergoes%20attenuation.%20The%20channel%20kinetics%20were%20modeled%20with%20four%20chemical%20states%20and%20the%20model%20predicts%20that%20it%20behaves%20as%20a%20pass%20band%20filter%20in%20the%2010%5Cu2009Hz-1%5Cu2009kHz%20range.%20In%20conclusion%2C%20due%20to%20its%20activation%5C%2Finactivation%2C%20the%20RMA%20channel%20is%20a%20candidate%20for%20mediating%20cytosolic%20calcium%20signaling%20in%20response%20to%20mechanostimulation.%20Its%20attenuation%20and%20filtering%20properties%20suggest%20its%20involvement%20in%20the%20transduction%20of%20high%20frequency%20mechanical%20stimulation%2C%20such%20as%20those%20produced%20by%20insects%26%23039%3B%20vibrations.%22%2C%22date%22%3A%222026-05-07%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1111%5C%2Fnph.71241%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242099044%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221469-8137%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A41%3A27Z%22%7D%7D%2C%7B%22key%22%3A%22M6FL75B2%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ishigami%20et%20al.%22%2C%22parsedDate%22%3A%222026-05-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BIshigami%2C%20Kota%2C%20Seonghan%20Jang%2C%20Aoba%20Yoshioka%2C%20et%20al.%202026.%20%26%23x201C%3BA%20Trojan%20Horse%20Pathogen%20Breaking%20through%20Partner-Choice%20Barriers%20in%20the%20Insect%20Gut.%26%23x201D%3B%20%26lt%3Bi%26gt%3BProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%26lt%3B%5C%2Fi%26gt%3B%20123%20%2818%29%3A%20e2533244123.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2533244123%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2533244123%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20Trojan%20horse%20pathogen%20breaking%20through%20partner-choice%20barriers%20in%20the%20insect%20gut%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kota%22%2C%22lastName%22%3A%22Ishigami%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Seonghan%22%2C%22lastName%22%3A%22Jang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aoba%22%2C%22lastName%22%3A%22Yoshioka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hiroyuki%22%2C%22lastName%22%3A%22Morimura%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aya%22%2C%22lastName%22%3A%22Yokota%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lionel%22%2C%22lastName%22%3A%22Moulin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antoine-Olivier%22%2C%22lastName%22%3A%22Lirette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kazutaka%22%2C%22lastName%22%3A%22Takeshita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daisuke%22%2C%22lastName%22%3A%22Nakane%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Mergaert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoshitomo%22%2C%22lastName%22%3A%22Kikuchi%22%7D%5D%2C%22abstractNote%22%3A%22Mutualistic%20symbioses%20are%20potentially%20vulnerable%20to%20exploitation%2C%20particularly%20in%20hosts%20that%20acquire%20symbionts%20from%20the%20environment%2C%20where%20harmful%20exploiters%20inhabit.%20The%20independent%20evolution%20and%20persistence%20of%20intricate%20partner-choice%20mechanisms%20in%20many%20symbioses%20testify%20the%20threat%20by%20specialized%20exploiters%20of%20mutualisms%2C%20although%20only%20few%20have%20been%20documented%20in%20nature.%20We%20report%20here%20a%20lethal%20%26quot%3BTrojan%20horse%26quot%3B%20pathogen%2C%20Burkholderia%20sp.%20SJ1%2C%20exploiting%20the%20stinkbug-Caballeronia%20gut%20symbiosis.%20This%20bacterium%20resembles%20symbionts%20by%20using%20wrapping%20motility%20to%20traverse%20the%20host%26%23039%3Bs%20sorting%20organ%2C%20inducing%20symbiotic%20organ%20morphogenesis%20and%20colonizing%20it.%20Unlike%20mutualists%2C%20however%2C%20it%20resists%20host%20digestion%20for%20nutrient%20acquisition%2C%20breaches%20the%20gut%20epithelium%2C%20and%20causes%20sepsis%2C%20rapidly%20killing%20the%20host.%20Colonization%20of%20the%20symbiotic%20organ%20is%20essential%20for%20its%20lethality.%20This%20case%20shows%20how%20pathogens%20can%20exploit%20mutualisms%2C%20highlighting%20the%20evolutionary%20pressures%20shaping%20partner-choice%20mechanisms%20and%20the%20fragility%20of%20even%20highly%20specialized%20mutualisms.%22%2C%22date%22%3A%222026-05-05%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2533244123%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242048464%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221091-6490%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-04-29T09%3A02%3A02Z%22%7D%7D%2C%7B%22key%22%3A%22IEDFJYAN%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hak%20et%20al.%22%2C%22parsedDate%22%3A%222026-05-03%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BHak%2C%20Fiona%2C%20Camille%20Marchet%2C%20Daniel%20Gautheret%2C%20and%20M%26%23xE9%3Blina%20Gallopin.%202026.%20%26%23x201C%3BMetappuccino%3A%20Large%20Language%20Model-Driven%20Reconstruction%20of%20Sequence%20Read%20Archive%20Metadata%20for%20Cancer%20Research.%26%23x201D%3B%20%26lt%3Bi%26gt%3BBioinformatics%26lt%3B%5C%2Fi%26gt%3B%20%28Oxford%2C%20England%29%2042%20%285%29%3A%20btag166.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbioinformatics%5C%2Fbtag166%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbioinformatics%5C%2Fbtag166%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Metappuccino%3A%20large%20language%20model-driven%20reconstruction%20of%20sequence%20read%20archive%20metadata%20for%20cancer%20research%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fiona%22%2C%22lastName%22%3A%22Hak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Marchet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22Gautheret%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00e9lina%22%2C%22lastName%22%3A%22Gallopin%22%7D%5D%2C%22abstractNote%22%3A%22MOTIVATION%3A%20High-throughput%20RNA%20sequencing%20has%20significantly%20advanced%20transcriptomic%20profiling%20in%20oncology.%20Millions%20of%20RNA-seq%20datasets%20have%20accumulated%20in%20public%20databases%20such%20as%20the%20Sequence%20Read%20Archive%20%28SRA%29.%20However%2C%20fragmented%2C%20ambiguous%2C%20or%20missing%20metadata%20can%20severely%20limit%20accurate%20cohort%20selection%2C%20introduce%20bias%2C%20and%20delay%20discoveries.%5CnRESULTS%3A%20To%20address%20these%20issues%2C%20we%20introduce%20%26%23039%3BMetappuccino%26%23039%3B%2C%20a%20hybrid%20metadata%20enrichment%20tool%20built%20on%20Mistral-7B-Instruct%20and%20specialized%20via%20low-rank%20adaptation%20%28LoRA%29.%20Metappuccino%20reconstructs%2019%20metadata%20classes%20%28e.g.%20organ%2C%20disease%2C%20cell%20type%29%20by%20combining%20deterministic%20extraction%5C%2Fnormalization%20with%20model-based%20completion%3A%204%20submission-mandatory%20fields%20are%20read%20directly%20from%20SRA%5C%2FAPI%20records%2C%20while%20the%20remaining%2015%20classes%20are%20obtained%20through%20validated%20rule-based%20extraction%20when%20explicitly%20supported%20by%20the%20context%20and%20otherwise%20predicted%20by%20the%20LoRA-specialized%20model%20when%20information%20is%20missing%20or%20ambiguous.%20To%20promote%20robust%2C%20context-aware%20inference%20rather%20than%20memorization%2C%20we%20designed%20training%20and%20data%20partitioning%20to%20minimize%20leakage%20and%20preserve%20generalization.%20When%20applicable%2C%20predicted%20values%20are%20mapped%20to%20standardized%20ontologies%20to%20ensure%20consistent%2C%20interoperable%20annotations.%20Across%20our%20benchmarks%2C%20Metappuccino%20substantially%20improves%20accuracy%20over%20the%20base%20model%2C%20matches%20or%20exceeds%20recent%20larger%20open-source%20LLMs%2C%20and%20reduces%20inference%20time%20by%20up%20to%20two-fold%20relative%20to%20these%20baselines.%20By%20enriching%20under-annotated%20public%20RNA-seq%20records%2C%20Metappuccino%20increases%20the%20usability%20of%20SRA%20datasets%20for%20large-scale%20reuse%2C%20with%20applications%20that%20extend%20beyond%20oncology%20transcriptomics.%5CnAVAILABILITY%20AND%20IMPLEMENTATION%3A%20Metappuccino%20source%20code%20is%20available%20on%3A%20github.com%5C%2Fchumphati%5C%2FMetappuccino.%20The%20fine-tuned%20LLM%2C%20MetappuccinoLLModel%2C%20is%20available%20on%3A%20huggingface.co%5C%2Fchumphati%5C%2FMetappuccinoLLModel.%20Both%20repositories%20are%20released%20under%20Apache-2.0%20license.%22%2C%22date%22%3A%222026-05-03%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fbioinformatics%5C%2Fbtag166%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242057294%22%2C%22PMCID%22%3A%22PMC13148957%22%2C%22ISSN%22%3A%221367-4811%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A46%3A55Z%22%7D%7D%2C%7B%22key%22%3A%22BUTTHQ5S%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Souaifan%20et%20al.%22%2C%22parsedDate%22%3A%222026-04-29%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BSouaifan%2C%20Hiba%2C%20Mickael%20Costallat%2C%20Laura%20Sitkiewicz%2C%20et%20al.%202026.%20%26%23x201C%3BTargeting%20of%20the%20Nuclear%20RNA%20Exosome%20to%20Chromatin%20by%20HP1%20Affects%20the%20Transcriptional%20Programs%20of%20Liver%20Cells.%26%23x201D%3B%20%26lt%3Bi%26gt%3BNature%20Communications%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20April%2029.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-026-72504-7%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-026-72504-7%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Targeting%20of%20the%20nuclear%20RNA%20exosome%20to%20chromatin%20by%20HP1%20affects%20the%20transcriptional%20programs%20of%20liver%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hiba%22%2C%22lastName%22%3A%22Souaifan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mickael%22%2C%22lastName%22%3A%22Costallat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Sitkiewicz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kylian%22%2C%22lastName%22%3A%22Godest%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Cammas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carl%22%2C%22lastName%22%3A%22Mann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22Muchardt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Rachez%22%7D%5D%2C%22abstractNote%22%3A%22Heterochromatin%20protein%201%20%28HP1%29%2C%20a%20hallmark%20of%20pericentromeric%20heterochromatin%2C%20is%20a%20chromatin-bound%20regulator%20of%20co-transcriptional%20processes%20including%20alternative%20splicing%2C%20but%20its%20role%20in%20RNA%20degradation%20remains%20unexplored.%20Here%2C%20we%20uncover%20a%20direct%20interaction%20between%20HP1%20and%20nuclear%20RNA%20exosome%20complexes%2C%20major%20RNA%20decay%20machineries.%20In%20mouse%20embryonic%20liver%20cells%2C%20inactivation%20of%20all%20three%20HP1%20isoforms%20leads%20to%20accumulation%20of%20retrotransposon-derived%20RNAs%20and%20stabilization%20of%20enhancer%20RNAs.%20These%20changes%20coincide%20with%20increased%20activity%20at%20a%20subset%20of%20liver%20enhancers%20particularly%20sensitive%20to%20reduced%20exosome%20activity%2C%20many%20of%20which%20regulate%20genes%20encoding%20extracellular%20matrix%20components%20such%20as%20collagen%20genes.%20Stratifying%20hepatocellular%20carcinoma%20samples%20by%20HP1%20expression%20further%20reveal%20that%20tumors%20with%20low%20HP1%20are%20marked%20by%20reduced%20RNA%20degradation%2C%20and%20increased%20expression%20of%20a%20similar%20subset%20of%20genes%20encoding%20extracellular%20matrix%20components%20and%20possibly%20contributing%20to%20tumor%20stiffness.%20These%20results%20suggest%20that%20HP1%26%23039%3Bs%20impact%20on%20RNA%20turnover%20contributes%20to%20its%20function%20in%20cancer%20biology.%22%2C%22date%22%3A%222026-04-29%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-026-72504-7%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242056110%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%222041-1723%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A45%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22ULRRCVI3%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nermont%20et%20al.%22%2C%22parsedDate%22%3A%222026-04-29%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BNermont%2C%20R%26%23xE9%3Bva%2C%20Saoussen%20Oueslati%2C%20Magali%20Aumont-Nicaise%2C%20Pascal%20Retailleau%2C%20Bogdan%20I.%20Iorga%2C%20and%20Thierry%20Naas.%202026.%20%26%23x201C%3BImproved%20Hydrolysis%20of%20Piperacillin%20by%20OXA-48-like%20R214G%20Variants%2C%20a%20Selective%20Advantage%20under%20Piperacillin-Tazobactam%20Exposure.%26%23x201D%3B%20%26lt%3Bi%26gt%3BAntimicrobial%20Agents%20and%20Chemotherapy%26lt%3B%5C%2Fi%26gt%3B%2C%20April%2029%2C%20e0126725.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faac.01267-25%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Faac.01267-25%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Improved%20hydrolysis%20of%20piperacillin%20by%20OXA-48-like%20R214G%20variants%2C%20a%20selective%20advantage%20under%20piperacillin-tazobactam%20exposure%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R%5Cu00e9va%22%2C%22lastName%22%3A%22Nermont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Saoussen%22%2C%22lastName%22%3A%22Oueslati%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Magali%22%2C%22lastName%22%3A%22Aumont-Nicaise%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22Retailleau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bogdan%20I.%22%2C%22lastName%22%3A%22Iorga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thierry%22%2C%22lastName%22%3A%22Naas%22%7D%5D%2C%22abstractNote%22%3A%22OXA-48-like%20carbapenemases%20have%20rapidly%20disseminated%20worldwide%2C%20becoming%20the%20most%20common%20carbapenemase%20in%20many%20countries%2C%20with%20more%20than%2060%20variants%20reported.%20Among%20them%2C%20OXA-244%20%28OXA-48-R214G%29%20and%20OXA-484%20%28OXA-181-R214G%29%20are%20increasingly%20reported%2C%20despite%20overall%20reduced%20hydrolytic%20activities%20for%20%5Cu03b2-lactams%2C%20including%20temocillin%20and%20carbapenems.%20R214%2C%20located%20in%20the%20%5Cu03b25-%5Cu03b26%20loop%2C%20through%20the%20interaction%20with%20D159%2C%20is%20crucial%20for%20carbapenem%20hydrolysis%20by%20structuring%20the%20active%20site.%20R214G%20variants%20of%20OXA-48-likes%20were%20analyzed%20by%20%5Cu03b2-lactam%20susceptibility%20testing%2C%20steady-state%20kinetic%20analyses%20in%20the%20presence%20or%20absence%20of%20sodium%20hydrogen%20carbonate%20%28NaHCO3%29%2C%20molecular%20modeling%2C%20X-ray%20crystallography%2C%20and%20protein%20stability%20assessments%20using%20differential%20scanning%20fluorimetry%20%28DSF%29.%20The%20R214G%20substitution%20in%20OXA-48%20and%20OXA-181%20results%20in%20reduced%20minimal%20inhibitory%20concentrations%20%28MICs%29%20for%20all%20%5Cu03b2-lactams%2C%20except%20for%20piperacillin%20and%20piperacillin%5C%2Ftazobactam%20combination%2C%20for%20which%20MICs%20are%20increased.%20OXA-244%20and%20OXA-484%20displayed%20a%20better%20affinity%20for%20piperacillin%20with%20lower%20Km%20than%20for%20parental%20enzymes%20and%20thus%20resulted%20in%20a%20higher%20catalytic%20efficiency%20for%20piperacillin%20hydrolysis.%20These%20results%20were%20supported%20by%20docking%20observations%2C%20highlighting%20enhanced%20affinity%20when%20glycine%20is%20located%20at%20position%20214.%20Overall%2C%20DSF%20indicated%20that%20the%20R214G%20substitution%2C%20while%20destabilizing%20the%20active%20site%2C%20enhances%20the%20global%20stability%20of%20the%20protein%2C%20especially%20for%20OXA-244.%20The%20addition%20of%20NaHCO3%20raised%20the%20activity%20and%20thermal%20stability%20of%20all%20enzymes%2C%20especially%20of%20OXA-48%2C%20which%20appeared%20more%20sensitive%20to%20the%20presence%20of%20NaHCO3.%20Although%20OXA-48-like%20R214G%20is%20considered%20a%20loss-of-function%20variant%2C%20our%20findings%20indicate%20it%20exhibits%20increased%20hydrolytic%20activity%20toward%20piperacillin%2C%20which%20may%20result%20in%20an%20advantage%20under%20piperacillin-tazobactam%20exposure%20and%20thus%20could%20contribute%20to%20the%20selection%20of%20these%20globally%20expanding%20variants.%22%2C%22date%22%3A%222026-04-29%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2Faac.01267-25%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242053451%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221098-6596%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A38%3A57Z%22%7D%7D%2C%7B%22key%22%3A%22JBIYL5BU%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Gigant%20et%20al.%22%2C%22parsedDate%22%3A%222026-04-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BGigant%2C%20Beno%26%23xEE%3Bt%2C%20Liza%20Ammar%20Khodja%2C%20Val%26%23xE9%3Brie%20Campanacci%2C%20and%20Guy%20Lippens.%202026.%20%26%23x201C%3BA%20Coherent%20Structural%20Picture%20of%20the%20Interaction%20of%20Tau%20with%20Tubulin%20Provides%20a%20Link%20to%20Its%20Aggregation.%26%23x201D%3B%20%26lt%3Bi%26gt%3BThe%20Journal%20of%20Biological%20Chemistry%26lt%3B%5C%2Fi%26gt%3B%2C%20April%2027%2C%20113086.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2026.113086%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jbc.2026.113086%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20coherent%20structural%20picture%20of%20the%20interaction%20of%20Tau%20with%20tubulin%20provides%20a%20link%20to%20its%20aggregation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beno%5Cu00eet%22%2C%22lastName%22%3A%22Gigant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Liza%20Ammar%22%2C%22lastName%22%3A%22Khodja%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Val%5Cu00e9rie%22%2C%22lastName%22%3A%22Campanacci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guy%22%2C%22lastName%22%3A%22Lippens%22%7D%5D%2C%22abstractNote%22%3A%22Tauopathies%20are%20a%20group%20of%20neurodegenerative%20diseases%20characterized%20by%20the%20presence%20of%20insoluble%20filaments%20of%20the%20Tau%20protein%20in%20the%20brain.%20In%20physiological%20conditions%2C%20Tau%20is%20involved%20in%20the%20regulation%20of%20microtubule%20dynamics.%20The%20study%20of%20its%20interaction%20with%20different%20tubulin%20assemblies%2C%20using%20various%20experimental%20approaches%2C%20leads%20to%20a%20seemingly%20disparate%20picture.%20Here%2C%20we%20propose%20to%20integrate%20this%20information%20into%20a%20model%20of%20how%20Tau%20participates%20in%20microtubule%20assembly%20and%20stabilization.%20Related%20to%20its%20intrinsically%20disordered%20nature%2C%20the%20binding%20of%20Tau%20to%20microtubules%20involves%20both%20specific%20interactions%2C%20along%20protofilaments%2C%20and%20non-specific%20ones%2C%20with%20the%20C-terminal%20region%20of%20tubulin%20subunits.%20In%20addition%2C%20the%20recent%20determination%20of%20a%20Tau%3Atubulin%20structure%20provides%20a%20model%20for%20a%20functional%20dimer%20of%20Tau%20targeting%20a%20microtubule%20aperture%20between%20protofilaments.%20Therefore%2C%20Tau%20regulates%20microtubule%20dynamics%20by%20modulating%20both%20longitudinal%20and%20lateral%20contacts.%20Finally%2C%20we%20discuss%20a%20possible%20connection%20of%20this%20dimer%20of%20Tau%20with%20its%20oligomerization%2C%20whether%20physiological%20or%20pathological.%22%2C%22date%22%3A%222026-04-27%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jbc.2026.113086%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242055333%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221083-351X%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-05-18T08%3A43%3A50Z%22%7D%7D%2C%7B%22key%22%3A%22XYPZY2TK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Diallinas%20and%20Scazzocchio%22%2C%22parsedDate%22%3A%222026-04-27%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BDiallinas%2C%20George%2C%20and%20Claudio%20Scazzocchio.%202026.%20%26%23x201C%3BA%2060-Year%20Journey%20with%20a%20Fungal%20Transporter%3A%20From%20Classical%20Genetics%20to%20Functional%2C%20Structural%2C%20and%20Evolutionary%20Insights.%26%23x201D%3B%20%26lt%3Bi%26gt%3BMicrobiology%20and%20Molecular%20Biology%20Reviews%3A%20MMBR%26lt%3B%5C%2Fi%26gt%3B%2C%20April%2027%2C%20e0036425.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmmbr.00364-25%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2Fmmbr.00364-25%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%2060-year%20journey%20with%20a%20fungal%20transporter%3A%20from%20classical%20genetics%20to%20functional%2C%20structural%2C%20and%20evolutionary%20insights%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22George%22%2C%22lastName%22%3A%22Diallinas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claudio%22%2C%22lastName%22%3A%22Scazzocchio%22%7D%5D%2C%22abstractNote%22%3A%22SUMMARYTransporters%20are%20transmembrane%20proteins%20that%20mediate%20the%20selective%20translocation%20of%20metabolites%2C%20ions%2C%20and%20drugs%20across%20biological%20membranes.%20Their%20activity%20is%20essential%20for%20cellular%20communication%2C%20nutrition%2C%20detoxification%2C%20homeostasis%2C%20and%20responses%20to%20stress.%20Despite%20their%20fundamental%20biological%20and%20biomedical%20importance%2C%20particularly%20their%20involvement%20in%20genetic%20diseases%20and%20multidrug%20resistance%20in%20microbes%20and%20cancer%20cells%2C%20transporters%20remained%20relatively%20understudied%20until%20recently.%20This%20was%20largely%20due%20to%20the%20technical%20challenges%20of%20isolating%20and%20functionally%20characterizing%20these%20dynamic%20proteins%2C%20whose%20structure%2C%20function%2C%20and%20cellular%20expression%20continuously%20depends%20on%20specific%20interactions%20with%20membrane%20lipids.%20Nevertheless%2C%20since%20the%20mid-1960s%2C%20several%20transporters%20have%20been%20identified%20and%20extensively%20characterized%20at%20the%20genetic%20and%20physiological%20levels%20in%20model%20microorganisms%2C%20including%20fungi.%20In%20this%20review%2C%20we%20trace%20the%2060-year%20research%20journey%20of%20UapA%2C%20a%20uric%20acid-xanthine%20transporter%20from%20the%20filamentous%20fungus%20Aspergillus%20nidulans%20%28Ascomycota%29.%20The%20UapA%20story%20spans%20from%20early%20classical%20genetic%20analyses%20to%20the%20recent%20determination%20of%20its%20high-resolution%20structure.%20We%20describe%20the%20development%20of%20genetic%2C%20molecular%2C%20and%20cellular%20tools%20and%20how%20these%20enabled%20the%20functional%20dissection%20of%20UapA%20and%20were%20subsequently%20used%20for%20other%20A.%20nidulans%20transporters%2C%20including%20studies%20on%20cellular%20expression%20and%20turnover%20regulation.%20We%20additionally%20highlight%20up-to-date%20structural%20approaches%20that%20refined%20our%20knowledge%20on%20the%20transport%20mechanism%2C%20how%20substrate%20specificity%20is%20determined%2C%20and%20on%20membrane%20trafficking%20pathways%20underlying%20biogenesis%20and%20endocytosis%20of%20UapA%20and%20other%20transporters.%20The%20concepts%20developed%20through%2060%20years%20of%20persistent%20work%20on%20UapA%20have%20established%20important%20paradigms%20relevant%20to%20fungal%20physiology%20that%20have%20proven%20to%20be%20broadly%20applicable%20to%20understanding%20transporter-linked%20processes%20in%20other%20eukaryotic%20organisms.%22%2C%22date%22%3A%222026-04-27%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1128%5C%2Fmmbr.00364-25%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242041252%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221098-5557%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-04-28T11%3A40%3A42Z%22%7D%7D%2C%7B%22key%22%3A%22T6QWDZKE%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Julien%20et%20al.%22%2C%22parsedDate%22%3A%222026-04-21%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BJulien%2C%20Manon%2C%20Sophie%20Zinn-Justin%2C%20and%20Francois-Xavier%20Theillet.%202026.%20%26%23x201C%3BAdvanced%20NMR%20Characterization%20and%20Sensitive%20Detection%20of%20Isoaspartate%20in%20Proteins.%26%23x201D%3B%20%26lt%3Bi%26gt%3BAnalytical%20Chemistry%26lt%3B%5C%2Fi%26gt%3B%2C%20ahead%20of%20print%2C%20April%2021.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.analchem.5c07290%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.analchem.5c07290%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Advanced%20NMR%20Characterization%20and%20Sensitive%20Detection%20of%20Isoaspartate%20in%20Proteins%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manon%22%2C%22lastName%22%3A%22Julien%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Zinn-Justin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Xavier%22%2C%22lastName%22%3A%22Theillet%22%7D%5D%2C%22abstractNote%22%3A%22Isoaspartate%20forms%20spontaneously%20from%20Asn%20deamidation%20or%20Asp%20isomerization%2C%20which%20can%20plague%20protein%20purification%20and%20storage%20processes.%20Indeed%2C%20it%20often%20comes%20with%20deleterious%20consequences%2C%20from%20a%20loss%20of%20function%20to%20a%20gain%20of%20toxic%20properties.%20IsoAsp%20detection%20is%20not%20straightforward%2C%20notably%20because%20it%20causes%20weak%20mass%20shifts%2C%20i.e.%2C%20%2B1%20or%200%20from%20the%20native%20Asn%20or%20Asp%2C%20respectively.%20NMR%20spectroscopy%20might%20help%20in%20nontargeted%20detection%20of%20isoAsp%2C%20but%20information%20on%20isoAsp%20NMR%20fingerprint%20and%20sensitive%20detection%20methods%20were%20missing.%20Here%2C%20we%20report%20the%20NMR%20characterization%20of%20isoAsp%20in%20ten%20model%2C%20random%20coil%20hexapeptides%2C%20and%20release%20reference%20chemical%20shifts%20and%20scalar%20couplings%20of%20backbone%20nuclei%20from%20%28i-1%29-%28i%29-%28i%2B1%29%20residues%20%28from%20283%20to%20310%20K%29.%20We%20show%20how%20isoAsp%20chemical%20shifts%20evolve%20with%20pH%20%28from%202%20to%208%29%20and%20urea%20concentration%20%28from%200%20to%208%20M%29.%20This%20led%20us%20to%20draw%20methods%20to%20identify%20isoAsp%20in%2013C%5C%2F15N-enriched%20and%20in%20natural%20abundance%20polypeptides.%20In%20the%20latter%20case%2C%20we%20use%20notably%20trypsinization%2C%20protein%20denaturation%2C%20and%201H-only%20NMR%2C%20enabling%20the%20detection%20of%2010%20nmol%20of%20isoAsp-containing%20protein%20in%201%20h.%20We%20exemplify%20this%20approach%20on%20therapeutic%20products%20like%20insulin%20or%20the%20monoclonal%20antibody%20trastuzumab.%22%2C%22date%22%3A%222026-04-21%22%2C%22section%22%3A%22%22%2C%22partNumber%22%3A%22%22%2C%22partTitle%22%3A%22%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.analchem.5c07290%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22%22%2C%22PMID%22%3A%2242011539%22%2C%22PMCID%22%3A%22%22%2C%22ISSN%22%3A%221520-6882%22%2C%22language%22%3A%22eng%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222026-04-22T08%3A54%3A57Z%22%7D%7D%5D%7D
Piet, Quentin, Pascale Besse, Stéphanie Bocs, et al. 2026. “A Chromosome-Level Genome Assembly of Vanilla Planifolia Uncovers the Genomic Architecture Underlying Partial Endoreplication.” BMC Genomics, ahead of print, May 13. https://doi.org/10.1186/s12864-026-12926-1.
Zahid, Sayma, Jeanne Chauvat, Ilaria Ceppi, et al. 2026. “Structural Basis of Ku-Mediated Activation of WRN Exonuclease Activity.” Nature Communications, ahead of print, May 13. https://doi.org/10.1038/s41467-026-71888-w.
Guerringue, Yannick, Sebastien Thomine, Jean-Marc Allain, and Jean-Marie Frachisse. 2026. “The Rapid Mechanically Activated Channel Transduces Increases in Plasma Membrane Tension into Transient Calcium Influx.” The New Phytologist, ahead of print, May 7. https://doi.org/10.1111/nph.71241.
Ishigami, Kota, Seonghan Jang, Aoba Yoshioka, et al. 2026. “A Trojan Horse Pathogen Breaking through Partner-Choice Barriers in the Insect Gut.” Proceedings of the National Academy of Sciences of the United States of America 123 (18): e2533244123. https://doi.org/10.1073/pnas.2533244123.
Hak, Fiona, Camille Marchet, Daniel Gautheret, and Mélina Gallopin. 2026. “Metappuccino: Large Language Model-Driven Reconstruction of Sequence Read Archive Metadata for Cancer Research.” Bioinformatics (Oxford, England) 42 (5): btag166. https://doi.org/10.1093/bioinformatics/btag166.
Souaifan, Hiba, Mickael Costallat, Laura Sitkiewicz, et al. 2026. “Targeting of the Nuclear RNA Exosome to Chromatin by HP1 Affects the Transcriptional Programs of Liver Cells.” Nature Communications, ahead of print, April 29. https://doi.org/10.1038/s41467-026-72504-7.
Nermont, Réva, Saoussen Oueslati, Magali Aumont-Nicaise, Pascal Retailleau, Bogdan I. Iorga, and Thierry Naas. 2026. “Improved Hydrolysis of Piperacillin by OXA-48-like R214G Variants, a Selective Advantage under Piperacillin-Tazobactam Exposure.” Antimicrobial Agents and Chemotherapy, April 29, e0126725. https://doi.org/10.1128/aac.01267-25.
Gigant, Benoît, Liza Ammar Khodja, Valérie Campanacci, and Guy Lippens. 2026. “A Coherent Structural Picture of the Interaction of Tau with Tubulin Provides a Link to Its Aggregation.” The Journal of Biological Chemistry, April 27, 113086. https://doi.org/10.1016/j.jbc.2026.113086.
Diallinas, George, and Claudio Scazzocchio. 2026. “A 60-Year Journey with a Fungal Transporter: From Classical Genetics to Functional, Structural, and Evolutionary Insights.” Microbiology and Molecular Biology Reviews: MMBR, April 27, e0036425. https://doi.org/10.1128/mmbr.00364-25.
Julien, Manon, Sophie Zinn-Justin, and Francois-Xavier Theillet. 2026. “Advanced NMR Characterization and Sensitive Detection of Isoaspartate in Proteins.” Analytical Chemistry, ahead of print, April 21. https://doi.org/10.1021/acs.analchem.5c07290.