Electronic Spectroscopy
Publications
3888256
SE
chicago-author-date
50
date
desc
year
32604
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-54d9ecebe6ed89e7e95c639efebfa084%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22S79DC4G8%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Streckaite%20et%20al.%22%2C%22parsedDate%22%3A%222024-07-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStreckaite%2C%20Simona%2C%20Cristian%20Ilioaia%2C%20Igor%20Chaussavoine%2C%20Jevgenij%20Chmeliov%2C%20Andrius%20Gelzinis%2C%20Dmitrij%20Frolov%2C%20Leonas%20Valkunas%2C%20Sylvie%20Rimsky%2C%20Andrew%20Gall%2C%20and%20Bruno%20Robert.%202024.%20%26%23x201C%3BFunctional%20Organization%20of%203D%20Plant%20Thylakoid%20Membranes%20as%20Seen%20by%20High%20Resolution%20Microscopy.%26%23x201D%3B%20%3Ci%3EBiochimica%20Et%20Biophysica%20Acta.%20Bioenergetics%3C%5C%2Fi%3E%2C%20July%2C%20149493.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbabio.2024.149493%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbabio.2024.149493%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Functional%20organization%20of%203D%20plant%20thylakoid%20membranes%20as%20seen%20by%20high%20resolution%20microscopy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Streckaite%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristian%22%2C%22lastName%22%3A%22Ilioaia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Igor%22%2C%22lastName%22%3A%22Chaussavoine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jevgenij%22%2C%22lastName%22%3A%22Chmeliov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrius%22%2C%22lastName%22%3A%22Gelzinis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dmitrij%22%2C%22lastName%22%3A%22Frolov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leonas%22%2C%22lastName%22%3A%22Valkunas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Rimsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%22%2C%22lastName%22%3A%22Gall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%5D%2C%22abstractNote%22%3A%22In%20the%20field%20of%20photosynthesis%2C%20only%20a%20limited%20number%20of%20approaches%20of%20super-resolution%20fluorescence%20microscopy%20can%20be%20used%2C%20as%20the%20functional%20architecture%20of%20the%20thylakoid%20membrane%20in%20chloroplasts%20is%20probed%20through%20the%20natural%20fluorescence%20of%20chlorophyll%20molecules.%20In%20this%20work%2C%20we%20have%20used%20a%20custom-built%20fluorescence%20microscopy%20method%20called%20Single%20Pixel%20Reconstruction%20Imaging%20%28SPiRI%29%20that%20yields%20a%201.4%20gain%20in%20lateral%20and%20axial%20resolution%20relative%20to%20confocal%20fluorescence%20microscopy%2C%20to%20obtain%202D%20images%20and%203D-reconstucted%20volumes%20of%20isolated%20chloroplasts%2C%20obtained%20from%20pea%20%28Pisum%20sativum%29%2C%20spinach%20%28Spinacia%20oleracea%29%20and%20Arabidopsis%20thaliana.%20In%20agreement%20with%20previous%20studies%2C%20SPiRI%20images%20exhibit%20larger%20thylakoid%20grana%20diameters%20when%20extracted%20from%20plants%20under%20low-light%20regimes.%20The%20three-dimensional%20thylakoid%20architecture%2C%20revealing%20the%20complete%20network%20of%20the%20thylakoid%20membrane%20in%20intact%2C%20non-chemically-fixed%20chloroplasts%20can%20be%20visualized%20from%20the%20volume%20reconstructions%20obtained%20at%20high%20resolution.%20From%20such%20reconstructions%2C%20the%20stromal%20connections%20between%20each%20granum%20can%20be%20determined%20and%20the%20fluorescence%20intensity%20in%20the%20stromal%20lamellae%20compared%20to%20those%20of%20neighboring%20grana.%22%2C%22date%22%3A%222024-07-04%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.bbabio.2024.149493%22%2C%22ISSN%22%3A%221879-2650%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-07-08T14%3A45%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22WE5HYTQQ%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Aleksandrov%20et%20al.%22%2C%22parsedDate%22%3A%222024-03-11%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAleksandrov%2C%20Alexey%2C%20Adeline%20Bonvalet%2C%20Pavel%20M%26%23xFC%3Bller%2C%20Damien%20Sorigu%26%23xE9%3B%2C%20Fred%20Beisson%2C%20Laura%20Antonucci%2C%20Xavier%20Solinas%2C%20Manuel%20Joffre%2C%20and%20Marten%20Vos.%202024.%20%26%23x201C%3BCatalytic%20Mechanism%20of%20Fatty%20Acid%20Photodecarboxylase%3A%20On%20the%20Detection%20and%20Stability%20of%20the%20Initial%20Carbonyloxy%20Radical%20Intermediate.%26%23x201D%3B%20%3Ci%3EAngewandte%20Chemie%20%28International%20Ed.%20in%20English%29%3C%5C%2Fi%3E%2C%20March%2C%20e202401376.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.202401376%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.202401376%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Catalytic%20Mechanism%20of%20Fatty%20Acid%20Photodecarboxylase%3A%20on%20the%20Detection%20and%20Stability%20of%20the%20Initial%20Carbonyloxy%20Radical%20Intermediate%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexey%22%2C%22lastName%22%3A%22Aleksandrov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adeline%22%2C%22lastName%22%3A%22Bonvalet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22M%5Cu00fcller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Damien%22%2C%22lastName%22%3A%22Sorigu%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fred%22%2C%22lastName%22%3A%22Beisson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Antonucci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xavier%22%2C%22lastName%22%3A%22Solinas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manuel%22%2C%22lastName%22%3A%22Joffre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marten%22%2C%22lastName%22%3A%22Vos%22%7D%5D%2C%22abstractNote%22%3A%22In%20fatty%20acid%20photodecarboxylase%20%28FAP%29%2C%20light-induced%20formation%20of%20the%20primary%20radical%20product%20RCOO%5Cu25cf%20from%20fatty%20acid%20RCOO-%20occurs%20in%20300%5Cu00a0ps%2C%20upon%20which%20CO2%20is%20released%20quasi-immediately.%20Based%20on%20the%20hypothesis%20that%20aliphatic%20RCOO%5Cu25cf%20%28spectroscopically%20uncharacterized%20because%20unstable%29%20absorbs%20in%20the%20red%20similarly%20to%20aromatic%20carbonyloxy%20radicals%20such%20as%202%2C6-dichlorobenzoyloxy%20radical%20%28DCB%5Cu25cf%29%2C%20much%20longer-lived%20linear%20RCOO%5Cu25cf%20has%20been%20suggested%20recently.%20We%20performed%20quantum%20chemical%20reaction%20pathway%20and%20spectral%20calculations.%20These%20calculations%20are%20in%20line%20with%20the%20experimental%20DCB%5Cu25cf%20decarboxylation%20dynamics%20and%20spectral%20properties%20and%20show%20that%20in%20contrast%20to%20DCB%5Cu25cf%2C%20aliphatic%20RCOO%5Cu25cf%20radicals%20a%29%20decarboxylate%20with%20a%20very%20low%20energetic%20barrier%20and%20on%20the%20timescale%20of%20a%20few%20ps%20and%20b%29%20exhibit%20little%20red%20absorption.%20A%20time-resolved%20infrared%20spectroscopy%20experiment%20confirms%20very%20rapid%2C%20%3C%3C300%5Cu00a0ps%20RCOO%5Cu25cf%20decarboxylation%20in%20FAP.%20We%20argue%20that%20this%20property%20is%20required%20for%20the%20observed%20high%20quantum%20yield%20of%20hydrocarbons%20formation%20by%20FAP.%22%2C%22date%22%3A%222024-03-11%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1002%5C%2Fanie.202401376%22%2C%22ISSN%22%3A%221521-3773%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-03-13T07%3A33%3A47Z%22%7D%7D%2C%7B%22key%22%3A%223CHIW9E7%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Renouard%20et%20al.%22%2C%22parsedDate%22%3A%222023-07-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERenouard%2C%20Emilie%2C%20Magdalena%20Nowinska%2C%20Fabien%20Lacombat%2C%20Pascal%20Plaza%2C%20Pavel%20M%26%23xFC%3Bller%2C%20and%20Agathe%20Espagne.%202023.%20%26%23x201C%3BMultiscale%20Transient%20Absorption%20Study%20of%20the%20Fluorescent%20Protein%20Dreiklang%20and%20Two%20Point%20Variants%20Provides%20Insight%20into%20Photoswitching%20and%20Nonproductive%20Reaction%20Pathways.%26%23x201D%3B%20%3Ci%3EThe%20Journal%20of%20Physical%20Chemistry%20Letters%3C%5C%2Fi%3E%2C%20July%2C%206477%26%23x2013%3B85.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jpclett.3c00431%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jpclett.3c00431%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Multiscale%20Transient%20Absorption%20Study%20of%20the%20Fluorescent%20Protein%20Dreiklang%20and%20Two%20Point%20Variants%20Provides%20Insight%20into%20Photoswitching%20and%20Nonproductive%20Reaction%20Pathways%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emilie%22%2C%22lastName%22%3A%22Renouard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Magdalena%22%2C%22lastName%22%3A%22Nowinska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabien%22%2C%22lastName%22%3A%22Lacombat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22Plaza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22M%5Cu00fcller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agathe%22%2C%22lastName%22%3A%22Espagne%22%7D%5D%2C%22abstractNote%22%3A%22Dreiklang%20is%20a%20reversibly%20photoswitchable%20fluorescent%20protein%20used%20as%20a%20probe%20in%20advanced%20fluorescence%20imaging.%20It%20undergoes%20a%20unique%20and%20still%20poorly%20understood%20photoswitching%20mechanism%20based%20on%20the%20reversible%20addition%20of%20a%20water%20molecule%20to%20the%20chromophore.%20We%20report%20the%20first%20comprehensive%20study%20of%20the%20dynamics%20of%20this%20reaction%20by%20transient%20absorption%20spectroscopy%20from%20100%20fs%20to%20seconds%20in%20the%20original%20Dreiklang%20protein%20and%20two%20point%20variants.%20The%20picture%20that%20emerges%20from%20our%20work%20is%20that%20of%20a%20competition%20between%20photoswitching%20and%20nonproductive%20reaction%20pathways.%20We%20found%20that%20photoswitching%20had%20a%20low%20quantum%20yield%20of%200.4%25.%20It%20involves%20electron%20transfer%20from%20a%20tyrosine%20residue%20%28Tyr203%29%20to%20the%20chromophore%20and%20is%20completed%20in%2033%20ns.%20Nonproductive%20deactivation%20pathways%20comprise%20recombination%20of%20a%20charge%20transfer%20intermediate%2C%20excited-state%20proton%20transfer%20from%20the%20chromophore%20to%20a%20histidine%20residue%20%28His145%29%2C%20and%20decay%20to%20the%20ground%20state%20via%20micro-%5C%2Fmillisecond-lived%20intermediates.%22%2C%22date%22%3A%222023-07-12%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.jpclett.3c00431%22%2C%22ISSN%22%3A%221948-7185%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-12-05T16%3A33%3A15Z%22%7D%7D%2C%7B%22key%22%3A%22QYISHC96%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Samire%20et%20al.%22%2C%22parsedDate%22%3A%222023-03-31%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESamire%2C%20Poutoum%20P.%2C%20Bo%20Zhuang%2C%20Bertrand%20L%26%23xE9%3Bgeret%2C%20%26%23xC1%3Bngel%20Baca-Porcel%2C%20Gilles%20Peltier%2C%20Damien%20Sorigu%26%23xE9%3B%2C%20Alexey%20Aleksandrov%2C%20Fr%26%23xE9%3Bd%26%23xE9%3Bric%20Beisson%2C%20and%20Pavel%20M%26%23xFC%3Bller.%202023.%20%26%23x201C%3BAutocatalytic%20Effect%20Boosts%20the%20Production%20of%20Medium-Chain%20Hydrocarbons%20by%20Fatty%20Acid%20Photodecarboxylase.%26%23x201D%3B%20%3Ci%3EScience%20Advances%3C%5C%2Fi%3E%209%20%2813%29%3A%20eadg3881.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.adg3881%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.adg3881%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Autocatalytic%20effect%20boosts%20the%20production%20of%20medium-chain%20hydrocarbons%20by%20fatty%20acid%20photodecarboxylase%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Poutoum%20P.%22%2C%22lastName%22%3A%22Samire%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bo%22%2C%22lastName%22%3A%22Zhuang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertrand%22%2C%22lastName%22%3A%22L%5Cu00e9geret%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22%5Cu00c1ngel%22%2C%22lastName%22%3A%22Baca-Porcel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Peltier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Damien%22%2C%22lastName%22%3A%22Sorigu%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexey%22%2C%22lastName%22%3A%22Aleksandrov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fr%5Cu00e9d%5Cu00e9ric%22%2C%22lastName%22%3A%22Beisson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22M%5Cu00fcller%22%7D%5D%2C%22abstractNote%22%3A%22Ongoing%20climate%20change%20is%20driving%20the%20search%20for%20renewable%20and%20carbon-neutral%20alternatives%20to%20fossil%20fuels.%20Photocatalytic%20conversion%20of%20fatty%20acids%20to%20hydrocarbons%20by%20fatty%20acid%20photodecarboxylase%20%28FAP%29%20represents%20a%20promising%20route%20to%20green%20fuels.%20However%2C%20the%20alleged%20low%20activity%20of%20FAP%20on%20C2%20to%20C12%20fatty%20acids%20seemed%20to%20preclude%20the%20use%20for%20synthesis%20of%20gasoline-range%20hydrocarbons.%20Here%2C%20we%20reveal%20that%20Chlorella%20variabilis%20FAP%20%28CvFAP%29%20can%20convert%20n-octanoic%20acid%20in%20vitro%20four%20times%20faster%20than%20n-hexadecanoic%20acid%2C%20its%20best%20substrate%20reported%20to%20date.%20In%20vivo%2C%20this%20translates%20into%20a%20CvFAP-based%20production%20rate%20over%2010-fold%20higher%20for%20n-heptane%20than%20for%20n-pentadecane.%20Time-resolved%20spectroscopy%20and%20molecular%20modeling%20demonstrate%20that%20CvFAP%27s%20high%20catalytic%20activity%20on%20n-octanoic%20acid%20is%2C%20in%20part%2C%20due%20to%20an%20autocatalytic%20effect%20of%20its%20n-heptane%20product%2C%20which%20fills%20the%20rest%20of%20the%20binding%20pocket.%20These%20results%20represent%20an%20important%20step%20toward%20a%20bio-based%20and%20light-driven%20production%20of%20gasoline-like%20hydrocarbons.%22%2C%22date%22%3A%222023-03-31%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1126%5C%2Fsciadv.adg3881%22%2C%22ISSN%22%3A%222375-2548%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-12-05T16%3A33%3A06Z%22%7D%7D%2C%7B%22key%22%3A%225V8IKJIY%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Messant%20et%20al.%22%2C%22parsedDate%22%3A%222023-02-01%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMessant%2C%20Marine%2C%20Umama%20Hani%2C%20Tha%26%23xEF%3Bs%20Hennebelle%2C%20Florence%20Gu%26%23xE9%3Brard%2C%20Bertrand%20Gaki%26%23xE8%3Bre%2C%20Andrew%20Gall%2C%20S%26%23xE9%3Bbastien%20Thomine%2C%20and%20Anja%20Krieger-Liszkay.%202023.%20%26%23x201C%3BManganese%20Concentration%20Affects%20Chloroplast%20Structure%20and%20the%20Photosynthetic%20Apparatus%20in%20Marchantia%20Polymorpha.%26%23x201D%3B%20%3Ci%3EPlant%20Physiology%3C%5C%2Fi%3E%2C%20February%2C%20kiad052.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fplphys%5C%2Fkiad052%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fplphys%5C%2Fkiad052%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Manganese%20concentration%20affects%20chloroplast%20structure%20and%20the%20photosynthetic%20apparatus%20in%20Marchantia%20polymorpha%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marine%22%2C%22lastName%22%3A%22Messant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Umama%22%2C%22lastName%22%3A%22Hani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tha%5Cu00efs%22%2C%22lastName%22%3A%22Hennebelle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Gu%5Cu00e9rard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertrand%22%2C%22lastName%22%3A%22Gaki%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%22%2C%22lastName%22%3A%22Gall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S%5Cu00e9bastien%22%2C%22lastName%22%3A%22Thomine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anja%22%2C%22lastName%22%3A%22Krieger-Liszkay%22%7D%5D%2C%22abstractNote%22%3A%22Manganese%20%28Mn%29%20is%20an%20essential%20metal%20for%20plant%20growth.%20The%20most%20important%20Mn-containing%20enzyme%20is%20the%20Mn4CaO5%20cluster%20that%20catalyzes%20water%20oxidation%20in%20Photosystem%20II.%20Mn%20deficiency%20primarily%20affects%20photosynthesis%2C%20while%20Mn%20excess%20is%20generally%20toxic.%20Here%2C%20we%20studied%20Mn%20excess%20and%20deficiency%20in%20the%20liverwort%20Marchantia%20polymorpha%2C%20an%20emerging%20model%20ideally%20suited%20for%20analysis%20of%20metal%20stress%20since%20it%20accumulates%20rapidly%20toxic%20substances%20due%20to%20the%20absence%20of%20well-developed%20vascular%20and%20radicular%20systems%20and%20a%20reduced%20cuticle.%20We%20established%20growth%20conditions%20for%20Mn%20excess%20and%20deficiency%20and%20analyzed%20the%20metal%20content%20in%20thalli%20and%20isolated%20chloroplasts.%20In%20vivo%20super-resolution%20fluorescence%20microscopy%20and%20transmission%20electron%20microscopy%20revealed%20changes%20in%20the%20organization%20of%20the%20thylakoid%20membrane%20under%20Mn%20excess%20and%20deficiency.%20Both%20Mn%20excess%20and%20Mn%20deficiency%20increased%20the%20stacking%20of%20the%20thylakoid%20membrane.%20We%20investigated%20photosynthetic%20performance%20by%20measuring%20chlorophyll%20fluorescence%20at%20room%20temperature%20and%2077%5Cu2005K%2C%20measuring%20P700%20absorbance%2C%20and%20studying%20the%20susceptibility%20of%20thalli%20to%20photoinhibition.%20Non-optimal%20Mn%20concentrations%20changed%20the%20ratio%20of%20photosystem%20I%20to%20photosystem%20II.%20Upon%20Mn%20deficiency%2C%20higher%20non-photochemical%20quenching%20was%20observed%2C%20electron%20donation%20to%20photosystem%20I%20was%20favored%2C%20and%20photosystem%20II%20was%20less%20susceptible%20to%20photoinhibition.%20Mn%20deficiency%20seemed%20to%20favor%20cyclic%20electron%20flow%20around%20photosystem%20I%2C%20thereby%20protecting%20photosystem%20II%20in%20high%20light.%20The%20results%20presented%20here%20suggest%20an%20important%20role%20of%20Mn%20for%20the%20organization%20of%20the%20thylakoid%20membrane%20and%20photosynthetic%20electron%20transport.%22%2C%22date%22%3A%222023-02-01%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fplphys%5C%2Fkiad052%22%2C%22ISSN%22%3A%221532-2548%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-12-05T16%3A32%3A59Z%22%7D%7D%2C%7B%22key%22%3A%22XCRURPWX%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Lacombat%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-11%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELacombat%2C%20Fabien%2C%20Agathe%20Espagne%2C%20Nadia%20Dozova%2C%20Pascal%20Plaza%2C%20Pavel%20M%26%23xFC%3Bller%2C%20Hans-Joachim%20Emmerich%2C%20Martin%20Saft%2C%20and%20Lars-Oliver%20Essen.%202021.%20%26%23x201C%3BUltrafast%20Photoreduction%20Dynamics%20of%20a%20New%20Class%20of%20CPD%20Photolyases.%26%23x201D%3B%20%3Ci%3EPhotochemical%20%26amp%3B%20Photobiological%20Sciences%3A%20Official%20Journal%20of%20the%20European%20Photochemistry%20Association%20and%20the%20European%20Society%20for%20Photobiology%3C%5C%2Fi%3E%2020%20%286%29%3A%20733%26%23x2013%3B46.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs43630-021-00048-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs43630-021-00048-4%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ultrafast%20photoreduction%20dynamics%20of%20a%20new%20class%20of%20CPD%20photolyases%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabien%22%2C%22lastName%22%3A%22Lacombat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agathe%22%2C%22lastName%22%3A%22Espagne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nadia%22%2C%22lastName%22%3A%22Dozova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22Plaza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pavel%22%2C%22lastName%22%3A%22M%5Cu00fcller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hans-Joachim%22%2C%22lastName%22%3A%22Emmerich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Saft%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lars-Oliver%22%2C%22lastName%22%3A%22Essen%22%7D%5D%2C%22abstractNote%22%3A%22NewPHL%20is%20a%20recently%20discovered%20subgroup%20of%20ancestral%20DNA%20photolyases.%20Its%20domain%20architecture%20displays%20pronounced%20differences%20from%20that%20of%20canonical%20photolyases%2C%20in%20particular%20at%20the%20level%20of%20the%20characteristic%20electron%20transfer%20chain%2C%20which%20is%20limited%20to%20merely%20two%20tryptophans%2C%20instead%20of%20the%20%5C%22classical%5C%22%20three%20or%20four.%20Using%20transient%20absorption%20spectroscopy%2C%20we%20show%20that%20the%20dynamics%20of%20photoreduction%20of%20the%20oxidized%20FAD%20cofactor%20in%20the%20NewPHL%20begins%20similarly%20as%20that%20in%20canonical%20photolyases%2C%20i.e.%2C%20with%20a%20sub-ps%20primary%20reduction%20of%20the%20excited%20FAD%20cofactor%20by%20an%20adjacent%20tryptophan%2C%20followed%20by%20migration%20of%20the%20electron%20hole%20towards%20the%20second%20tryptophan%20in%20the%20tens%20of%20ps%20regime.%20However%2C%20the%20resulting%20tryptophanyl%20radical%20then%20undergoes%20an%20unprecedentedly%20fast%20deprotonation%20in%20less%20than%20100%5Cu00a0ps%20in%20the%20NewPHL.%20In%20spite%20of%20the%20stabilization%20effect%20of%20this%20deprotonation%2C%20almost%20complete%20charge%20recombination%20follows%20in%20two%20phases%20of%5Cu2009~%5Cu2009950%5Cu00a0ps%20and%5Cu2009~%5Cu200950%5Cu00a0ns.%20Such%20a%20rapid%20recombination%20of%20the%20radical%20pair%20implies%20that%20the%20first%20FAD%20photoreduction%20step%2C%20i.e.%2C%20conversion%20of%20the%20fully%20oxidized%20to%20the%20semi-quinone%20state%2C%20should%20be%20rather%20difficult%20in%20vivo.%20We%20hence%20suggest%20that%20the%20flavin%20chromophore%20likely%20switches%20only%20between%20its%20semi-reduced%20and%20fully%20reduced%20form%20in%20NewPHL%20under%20physiological%20conditions.%22%2C%22date%22%3A%222021-05-11%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1007%5C%2Fs43630-021-00048-4%22%2C%22ISSN%22%3A%221474-9092%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%227EGDQ8LV%22%5D%2C%22dateModified%22%3A%222022-06-07T11%3A55%3A24Z%22%7D%7D%2C%7B%22key%22%3A%22FI3XJRTN%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Streckait%5Cu00e9%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EStreckait%26%23xE9%3B%2C%20Simona%2C%20Manuel%20J.%20Llansola-Portoles%2C%20Andrew%20A.%20Pascal%2C%20Cristian%20Ilioaia%2C%20Andrew%20Gall%2C%20Soichiro%20Seki%2C%20Ritsuko%20Fujii%2C%20and%20Bruno%20Robert.%202021.%20%26%23x201C%3BPigment%20Structure%20in%20the%20Light-Harvesting%20Protein%20of%20the%20Siphonous%20Green%20Alga%20Codium%20Fragile.%26%23x201D%3B%20%3Ci%3EBiochimica%20Et%20Biophysica%20Acta.%20Bioenergetics%3C%5C%2Fi%3E%201862%20%285%29%3A%20148384.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbabio.2021.148384%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bbabio.2021.148384%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pigment%20structure%20in%20the%20light-harvesting%20protein%20of%20the%20siphonous%20green%20alga%20Codium%20fragile%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Streckait%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manuel%20J.%22%2C%22lastName%22%3A%22Llansola-Portoles%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20A.%22%2C%22lastName%22%3A%22Pascal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristian%22%2C%22lastName%22%3A%22Ilioaia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%22%2C%22lastName%22%3A%22Gall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Soichiro%22%2C%22lastName%22%3A%22Seki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ritsuko%22%2C%22lastName%22%3A%22Fujii%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%5D%2C%22abstractNote%22%3A%22The%20siphonaxanthin-siphonein-chlorophyll-a%5C%2Fb-binding%20protein%20%28SCP%29%2C%20a%20trimeric%20light-harvesting%20complex%20isolated%20from%20photosystem%20II%20of%20the%20siphonous%20green%20alga%20Codium%20fragile%2C%20binds%20the%20carotenoid%20siphonaxanthin%20%28Sx%29%20and%5C%2For%20its%20ester%20siphonein%20in%20place%20of%20lutein%2C%20in%20addition%20to%20chlorophylls%20a%5C%2Fb%20and%20neoxanthin.%20SCP%20exhibits%20a%20higher%20content%20of%20chlorophyll%20b%20%28Chl-b%29%20than%20its%20counterpart%20in%20green%20plants%2C%20light-harvesting%20complex%20II%20%28LHCII%29%2C%20increasing%20the%20relative%20absorption%20of%20blue-green%20light%20for%20photosynthesis.%20Using%20low%20temperature%20absorption%20and%20resonance%20Raman%20spectroscopies%2C%20we%20reveal%20the%20presence%20of%20two%20non-equivalent%20Sx%20molecules%20in%20SCP%2C%20and%20assign%20their%20absorption%20peaks%20at%20501%20and%20535%5Cu00a0nm.%20The%20red-absorbing%20Sx%20population%20exhibits%20a%20significant%20distortion%20that%20is%20reminiscent%20of%20lutein%202%20in%20trimeric%20LHCII.%20Unexpected%20enhancement%20of%20the%20Raman%20modes%20of%20Chls-b%20in%20SCP%20allows%20an%20unequivocal%20description%20of%20seven%20to%20nine%20non-equivalent%20Chls-b%2C%20and%20six%20distinct%20Chl-a%20populations%20in%20this%20protein.%22%2C%22date%22%3A%222021-02-03%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.bbabio.2021.148384%22%2C%22ISSN%22%3A%221879-2650%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%227EGDQ8LV%22%5D%2C%22dateModified%22%3A%222021-09-10T12%3A33%3A03Z%22%7D%7D%2C%7B%22key%22%3A%222GFX9624%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chenebault%20et%20al.%22%2C%22parsedDate%22%3A%222020%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChenebault%2C%20C%26%23xE9%3Blia%2C%20Encarnaci%26%23xF3%3Bn%20Diaz-Santos%2C%20Xavier%20Kammerscheit%2C%20Sigrid%20G%26%23xF6%3Brgen%2C%20Cristian%20Ilioaia%2C%20Simona%20Streckaite%2C%20Andrew%20Gall%2C%20et%20al.%202020.%20%26%23x201C%3BA%20Genetic%20Toolbox%20for%20the%20New%20Model%20Cyanobacterium%20Cyanothece%20PCC%207425%3A%20A%20Case%20Study%20for%20the%20Photosynthetic%20Production%20of%20Limonene.%26%23x201D%3B%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2011%3A586601.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2020.586601%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2020.586601%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20Genetic%20Toolbox%20for%20the%20New%20Model%20Cyanobacterium%20Cyanothece%20PCC%207425%3A%20A%20Case%20Study%20for%20the%20Photosynthetic%20Production%20of%20Limonene%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9lia%22%2C%22lastName%22%3A%22Chenebault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Encarnaci%5Cu00f3n%22%2C%22lastName%22%3A%22Diaz-Santos%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xavier%22%2C%22lastName%22%3A%22Kammerscheit%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigrid%22%2C%22lastName%22%3A%22G%5Cu00f6rgen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristian%22%2C%22lastName%22%3A%22Ilioaia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simona%22%2C%22lastName%22%3A%22Streckaite%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%22%2C%22lastName%22%3A%22Gall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elodie%22%2C%22lastName%22%3A%22Marcon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David-Alexandre%22%2C%22lastName%22%3A%22Buisson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karim%22%2C%22lastName%22%3A%22Benzerara%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Fran%5Cu00e7ois%22%2C%22lastName%22%3A%22Sassi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Corinne%22%2C%22lastName%22%3A%22Cassier-Chauvat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Franck%22%2C%22lastName%22%3A%22Chauvat%22%7D%5D%2C%22abstractNote%22%3A%22Cyanobacteria%2C%20the%20largest%20phylum%20of%20prokaryotes%2C%20perform%20oxygenic%20photosynthesis%20and%20are%20regarded%20as%20the%20ancestors%20of%20the%20plant%20chloroplast%20and%20the%20purveyors%20of%20the%20oxygen%20and%20biomass%20that%20shaped%20the%20biosphere.%20Nowadays%2C%20cyanobacteria%20are%20attracting%20a%20growing%20interest%20in%20being%20able%20to%20use%20solar%20energy%2C%20H2O%2C%20CO2%20and%20minerals%20to%20produce%20biotechnologically%20interesting%20chemicals.%20This%20often%20requires%20the%20introduction%20and%20expression%20of%20heterologous%20genes%20encoding%20the%20enzymes%20that%20are%20not%20present%20in%20natural%20cyanobacteria.%20However%2C%20only%20a%20handful%20of%20model%20strains%20with%20a%20well-established%20genetic%20system%20are%20being%20studied%20so%20far%2C%20leaving%20the%20vast%20biodiversity%20of%20cyanobacteria%20poorly%20understood%20and%20exploited.%20In%20this%20study%2C%20we%20focused%20on%20the%20robust%20unicellular%20cyanobacterium%20Cyanothece%20PCC%207425%20that%20has%20many%20interesting%20attributes%2C%20such%20as%20large%20cell%20size%3B%20capacity%20to%20fix%20atmospheric%20nitrogen%20%28under%20anaerobiosis%29%20and%20to%20grow%20not%20only%20on%20nitrate%20but%20also%20on%20urea%20%28a%20frequent%20pollutant%29%20as%20the%20sole%20nitrogen%20source%3B%20capacity%20to%20form%20CO2-sequestrating%20intracellular%20calcium%20carbonate%20granules%20and%20to%20produce%20various%20biotechnologically%20interesting%20products.%20We%20demonstrate%20for%20the%20first%20time%20that%20RSF1010-derived%20plasmid%20vectors%20can%20be%20used%20for%20promoter%20analysis%2C%20as%20well%20as%20constitutive%20or%20temperature-controlled%20overproduction%20of%20proteins%20and%20analysis%20of%20their%20sub-cellular%20localization%20in%20Cyanothece%20PCC%207425.%20These%20findings%20are%20important%20because%20no%20gene%20manipulation%20system%20had%20been%20developed%20for%20Cyanothece%20PCC%207425%2C%20yet%2C%20handicapping%20its%20potential%20to%20serve%20as%20a%20model%20host.%20Furthermore%2C%20using%20this%20toolbox%2C%20we%20engineered%20Cyanothece%20PCC%207425%20to%20produce%20the%20high-value%20terpene%2C%20limonene%20which%20has%20applications%20in%20biofuels%2C%20bioplastics%2C%20cosmetics%2C%20food%20and%20pharmaceutical%20industries.%20This%20is%20the%20first%20report%20of%20the%20engineering%20of%20a%20Cyanothece%20strain%20for%20the%20production%20of%20a%20chemical%20and%20the%20first%20demonstration%20that%20terpene%20can%20be%20produced%20by%20an%20engineered%20cyanobacterium%20growing%20on%20urea%20as%20the%20sole%20nitrogen%20source.%22%2C%22date%22%3A%222020%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2020.586601%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%227EGDQ8LV%22%2C%2254WS7MX2%22%2C%22D373TGAV%22%5D%2C%22dateModified%22%3A%222023-03-21T12%3A17%3A29Z%22%7D%7D%2C%7B%22key%22%3A%22MZN9VR2T%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Arteni%20et%20al.%22%2C%22parsedDate%22%3A%222015-08-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EArteni%2C%20Ana-Andreea%2C%20Mathias%20Fradot%2C%20Denise%20Galzerano%2C%20Maria%20M.%20Mendes-Pinto%2C%20Jos%26%23xE9%3B-Alain%20Sahel%2C%20Serge%20Picaud%2C%20Bruno%20Robert%2C%20and%20Andrew%20A.%20Pascal.%202015.%20%26%23x201C%3BStructure%20and%20Conformation%20of%20the%20Carotenoids%20in%20Human%20Retinal%20Macular%20Pigment.%26%23x201D%3B%20Edited%20by%20Wayne%20Iwan%20Lee%20Davies.%20%3Ci%3EPLOS%20ONE%3C%5C%2Fi%3E%2010%20%288%29%3A%20e0135779.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0135779%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0135779%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structure%20and%20Conformation%20of%20the%20Carotenoids%20in%20Human%20Retinal%20Macular%20Pigment%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ana-Andreea%22%2C%22lastName%22%3A%22Arteni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathias%22%2C%22lastName%22%3A%22Fradot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denise%22%2C%22lastName%22%3A%22Galzerano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20M.%22%2C%22lastName%22%3A%22Mendes-Pinto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jos%5Cu00e9-Alain%22%2C%22lastName%22%3A%22Sahel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Serge%22%2C%22lastName%22%3A%22Picaud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bruno%22%2C%22lastName%22%3A%22Robert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrew%20A.%22%2C%22lastName%22%3A%22Pascal%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Wayne%20Iwan%20Lee%22%2C%22lastName%22%3A%22Davies%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222015-8-27%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0135779%22%2C%22ISSN%22%3A%221932-6203%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fdx.plos.org%5C%2F10.1371%5C%2Fjournal.pone.0135779%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-22T11%3A43%3A33Z%22%7D%7D%5D%7D
Streckaite, Simona, Cristian Ilioaia, Igor Chaussavoine, Jevgenij Chmeliov, Andrius Gelzinis, Dmitrij Frolov, Leonas Valkunas, Sylvie Rimsky, Andrew Gall, and Bruno Robert. 2024. “Functional Organization of 3D Plant Thylakoid Membranes as Seen by High Resolution Microscopy.” Biochimica Et Biophysica Acta. Bioenergetics, July, 149493. https://doi.org/10.1016/j.bbabio.2024.149493.
Aleksandrov, Alexey, Adeline Bonvalet, Pavel Müller, Damien Sorigué, Fred Beisson, Laura Antonucci, Xavier Solinas, Manuel Joffre, and Marten Vos. 2024. “Catalytic Mechanism of Fatty Acid Photodecarboxylase: On the Detection and Stability of the Initial Carbonyloxy Radical Intermediate.” Angewandte Chemie (International Ed. in English), March, e202401376. https://doi.org/10.1002/anie.202401376.
Renouard, Emilie, Magdalena Nowinska, Fabien Lacombat, Pascal Plaza, Pavel Müller, and Agathe Espagne. 2023. “Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways.” The Journal of Physical Chemistry Letters, July, 6477–85. https://doi.org/10.1021/acs.jpclett.3c00431.
Samire, Poutoum P., Bo Zhuang, Bertrand Légeret, Ángel Baca-Porcel, Gilles Peltier, Damien Sorigué, Alexey Aleksandrov, Frédéric Beisson, and Pavel Müller. 2023. “Autocatalytic Effect Boosts the Production of Medium-Chain Hydrocarbons by Fatty Acid Photodecarboxylase.” Science Advances 9 (13): eadg3881. https://doi.org/10.1126/sciadv.adg3881.
Messant, Marine, Umama Hani, Thaïs Hennebelle, Florence Guérard, Bertrand Gakière, Andrew Gall, Sébastien Thomine, and Anja Krieger-Liszkay. 2023. “Manganese Concentration Affects Chloroplast Structure and the Photosynthetic Apparatus in Marchantia Polymorpha.” Plant Physiology, February, kiad052. https://doi.org/10.1093/plphys/kiad052.
Lacombat, Fabien, Agathe Espagne, Nadia Dozova, Pascal Plaza, Pavel Müller, Hans-Joachim Emmerich, Martin Saft, and Lars-Oliver Essen. 2021. “Ultrafast Photoreduction Dynamics of a New Class of CPD Photolyases.” Photochemical & Photobiological Sciences: Official Journal of the European Photochemistry Association and the European Society for Photobiology 20 (6): 733–46. https://doi.org/10.1007/s43630-021-00048-4.
Streckaité, Simona, Manuel J. Llansola-Portoles, Andrew A. Pascal, Cristian Ilioaia, Andrew Gall, Soichiro Seki, Ritsuko Fujii, and Bruno Robert. 2021. “Pigment Structure in the Light-Harvesting Protein of the Siphonous Green Alga Codium Fragile.” Biochimica Et Biophysica Acta. Bioenergetics 1862 (5): 148384. https://doi.org/10.1016/j.bbabio.2021.148384.
Chenebault, Célia, Encarnación Diaz-Santos, Xavier Kammerscheit, Sigrid Görgen, Cristian Ilioaia, Simona Streckaite, Andrew Gall, et al. 2020. “A Genetic Toolbox for the New Model Cyanobacterium Cyanothece PCC 7425: A Case Study for the Photosynthetic Production of Limonene.” Frontiers in Microbiology 11:586601. https://doi.org/10.3389/fmicb.2020.586601.
Arteni, Ana-Andreea, Mathias Fradot, Denise Galzerano, Maria M. Mendes-Pinto, José-Alain Sahel, Serge Picaud, Bruno Robert, and Andrew A. Pascal. 2015. “Structure and Conformation of the Carotenoids in Human Retinal Macular Pigment.” Edited by Wayne Iwan Lee Davies. PLOS ONE 10 (8): e0135779. https://doi.org/10.1371/journal.pone.0135779.