Bacteriophage T5
Publications
3888256
T5PHAG
chicago-author-date
50
date
desc
year
10804
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
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Rossier, Ombeline, Cécile Labarre, Anne Lopes, Monique Auberdiac, Kevin Tambosco, Daniel Delaruelle, Hakima Abes, et al. 2024. “Genome Sequence of PSonyx, a Singleton Bacteriophage Infecting Corynebacterium Glutamicum.” Microbiology Resource Announcements, January, e0115523. https://doi.org/10.1128/mra.01155-23.
Vernhes, Emeline, Linda Larbi Chérif, Nicolas Ducrot, Clément Vanbergue, Malika Ouldali, Lena Zig, N’diaye Sidibe, et al. 2024. “Antigen Self-Anchoring onto Bacteriophage T5 Capsid-like Particles for Vaccine Design.” NPJ Vaccines 9 (1): 6. https://doi.org/10.1038/s41541-023-00798-5.
Alhaddad, Samer, Houda Bey, Olivier Thouvenin, Pascale Boulanger, Claude Boccara, Martine Boccara, and Ignacio Izeddin. 2023. “Real-Time Detection of Virus Antibody Interaction by Label-Free Common-Path Interferometry.” Biophysical Reports 3 (3): 100119. https://doi.org/10.1016/j.bpr.2023.100119.
Ramirez-Chamorro, Luis, Pascale Boulanger, and Ombeline Rossier. 2021. “Strategies for Bacteriophage T5 Mutagenesis: Expanding the Toolbox for Phage Genome Engineering.” Frontiers in Microbiology 12 (April):667332. https://doi.org/10.3389/fmicb.2021.667332.
Ansaldi, Mireille, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Rémy Froissart, Sylvain Gandon, et al. 2020. “Antibacterial applications of bacteriophages.” Virologie 24 (1): 23–36. https://doi.org/10.1684/vir.2019.0805.
Ansaldi, Mireille, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Rémy Froissart, Sylvain Gandon, et al. 2020. “A century of research on bacteriophages.” Virologie 24 (1): 9–22. https://doi.org/10.1684/vir.2020.0809.
Huet, Alexis, Robert L. Duda, Pascale Boulanger, and James F. Conway. 2019. “Capsid Expansion of Bacteriophage T5 Revealed by High Resolution Cryoelectron Microscopy.” Proceedings of the National Academy of Sciences of the United States of America 116 (42): 21037–46. https://doi.org/10.1073/pnas.1909645116.
Weiss, Victor U., Ronja Pogan, Samuele Zoratto, Kevin M. Bond, Pascale Boulanger, Martin F. Jarrold, Nicholas Lyktey, et al. 2019. “Virus-like Particle Size and Molecular Weight/Mass Determination Applying Gas-Phase Electrophoresis (Native NES GEMMA).” Analytical and Bioanalytical Chemistry 411 (23): 5951–62. https://doi.org/10.1007/s00216-019-01998-6.
Dominguez-Medina, Sergio, Shawn Fostner, Martial Defoort, Marc Sansa, Ann-Kathrin Stark, Mohammad Abdul Halim, Emeline Vernhes, et al. 2018. “Neutral Mass Spectrometry of Virus Capsids above 100 Megadaltons with Nanomechanical Resonators.” Science (New York, N.Y.) 362 (6417): 918–22. https://doi.org/10.1126/science.aat6457.
Ansaldi, Mireille, Laurent Debarbieux, Sylvain Gandon, Marie-Agnès Petit, Paulo Tavares, and Pascale Boulanger. 2018. “‘French Phage Network’-Third Meeting Report.” Viruses 10 (3). https://doi.org/10.3390/v10030123.
Hermouet, Maxime, Marc Sansa, Martial Defoort, Louise Banniard, Sergio Dominguez-Medina, Shawn Fostner, Ujwol Palanchoke, et al. 2018. “Very Large Scale Integration Optomechanics: A Cure for Loneliness of NEMS Resonators?” In 2018 Ieee International Electron Devices Meeting (Iedm). New York: Ieee.
Arnaud, Charles-Adrien, Grégory Effantin, Corinne Vivès, Sylvain Engilberge, Maria Bacia, Pascale Boulanger, Eric Girard, Guy Schoehn, and Cécile Breyton. 2017. “Bacteriophage T5 Tail Tube Structure Suggests a Trigger Mechanism for Siphoviridae DNA Ejection.” Nature Communications 8 (1): 1953. https://doi.org/10.1038/s41467-017-02049-3.
Vernhes, Emeline, Madalena Renouard, Bernard Gilquin, Philippe Cuniasse, Dominique Durand, Patrick England, Sylviane Hoos, et al. 2017. “Erratum: High Affinity Anchoring of the Decoration Protein Pb10 onto the Bacteriophage T5 Capsid.” Scientific Reports 7 (April):43977. https://doi.org/10.1038/srep43977.
Vernhes, Emeline, Madalena Renouard, Bernard Gilquin, Philippe Cuniasse, Dominique Durand, Patrick England, Sylviane Hoos, et al. 2017. “High Affinity Anchoring of the Decoration Protein Pb10 onto the Bacteriophage T5 Capsid.” Scientific Reports 7 (February):41662. https://doi.org/10.1038/srep41662.
Huet, Alexis, Robert L. Duda, Roger W. Hendrix, Pascale Boulanger, and James F. Conway. 2016. “Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex.” Journal of Molecular Biology 428 (1): 165–81. https://doi.org/10.1016/j.jmb.2015.11.019.
Frutos, Marta de, Amélie Leforestier, Jéril Degrouard, Nebraska Zambrano, Frank Wien, Pascale Boulanger, Sandrine Brasilès, Madalena Renouard, Dominique Durand, and Françoise Livolant. 2016. “Can Changes in Temperature or Ionic Conditions Modify the DNA Organization in the Full Bacteriophage Capsid?” The Journal of Physical Chemistry. B 120 (26): 5975–86. https://doi.org/10.1021/acs.jpcb.6b01783.
Garcia-Doval, Carmela, José R. Castón, Daniel Luque, Meritxell Granell, José M. Otero, Antonio L. Llamas-Saiz, Madalena Renouard, Pascale Boulanger, and Mark J. van Raaij. 2015. “Structure of the Receptor-Binding Carboxy-Terminal Domain of the Bacteriophage T5 L-Shaped Tail Fibre with and without Its Intra-Molecular Chaperone.” Viruses 7 (12): 6424–40. https://doi.org/10.3390/v7122946.