Evolution and Maintenance
of Circular Chromosomes
Publications
3888256
EMC2
chicago-author-date
50
date
desc
year
14099
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-b33c253df8dac5382c8c4c2f979fba73%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ZW8D94LN%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Besombes%20et%20al.%22%2C%22parsedDate%22%3A%222024-09-18%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBesombes%2C%20Amelie%2C%20Yazid%20Adam%2C%20Christophe%20Possoz%2C%20Ivan%20Junier%2C%20Francois-Xavier%20Barre%2C%20and%20Jean-Luc%20Ferat.%202024.%20%26%23x201C%3BDciA%20Secures%20Bidirectional%20Replication%20Initiation%20in%20Vibrio%20Cholerae.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2C%20September%2C%20gkae795.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae795%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae795%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22DciA%20secures%20bidirectional%20replication%20initiation%20in%20Vibrio%20cholerae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amelie%22%2C%22lastName%22%3A%22Besombes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yazid%22%2C%22lastName%22%3A%22Adam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Junier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%5D%2C%22abstractNote%22%3A%22Replication%20is%20initiated%20bidirectionally%20in%20the%20three%20domains%20of%20life%20by%20the%20assembly%20of%20two%20replication%20forks%20at%20an%20origin%20of%20replication.%20This%20is%20made%20possible%20by%20the%20recruitment%20of%20two%20replicative%20helicases%20to%20a%20nucleoprotein%20platform%20built%20at%20the%20origin%20of%20replication%20with%20the%20initiator%20protein.%20The%20reason%20why%20replication%20is%20initiated%20bidirectionally%20has%20never%20been%20experimentally%20addressed%20due%20to%20the%20lack%20of%20a%20suitable%20biological%20system.%20Using%20genetic%20and%20genomic%20approaches%2C%20we%20show%20that%20upon%20depletion%20of%20DciA%2C%20replication%20is%20no%20longer%20initiated%20bidirectionally%20at%20the%20origin%20of%20replication%20of%20Vibrio%20cholerae%20chromosome%201.%20We%20show%20that%20following%20unidirectional%20replication%20on%20the%20left%20replichore%2C%20nascent%20DNA%20strands%20at%20ori1%20anneal%20to%20each%20other%20to%20form%20a%20double-stranded%20DNA%20end.%20While%20this%20DNA%20end%20can%20be%20efficiently%20resected%20in%20recB%2B%20cells%2C%20only%20a%20few%20cells%20use%20it%20to%20trigger%20replication%20on%20the%20right%20replichore.%20In%20most%20DciA-depleted%20cells%2C%20chromosome%201%20is%20degraded%20leading%20to%20cell%20death.%20Our%20results%20suggest%20that%20DciA%20is%20essential%20to%20ensuring%20bidirectional%20initiation%20of%20replication%20in%20bacteria%2C%20preventing%20a%20cascade%20of%20deleterious%20events%20following%20unidirectional%20replication%20initiation.%22%2C%22date%22%3A%222024-09-18%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkae795%22%2C%22ISSN%22%3A%221362-4962%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-09-19T07%3A33%3A39Z%22%7D%7D%2C%7B%22key%22%3A%22KHNSUX74%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Goudin%20et%20al.%22%2C%22parsedDate%22%3A%222023%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGoudin%2C%20Anthony%2C%20Jean-Luc%20Ferat%2C%20Christophe%20Possoz%2C%20Fran%26%23xE7%3Bois-Xavier%20Barre%2C%20and%20Elisa%20Galli.%202023.%20%26%23x201C%3BRecovery%20of%20Vibrio%20Cholerae%20Polarized%20Cellular%20Organization%20after%20Exit%20from%20a%20Non-Proliferating%20Spheroplast%20State.%26%23x201D%3B%20%3Ci%3EPloS%20One%3C%5C%2Fi%3E%2018%20%2810%29%3A%20e0293276.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0293276%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pone.0293276%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Recovery%20of%20Vibrio%20cholerae%20polarized%20cellular%20organization%20after%20exit%20from%20a%20non-proliferating%20spheroplast%20state%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anthony%22%2C%22lastName%22%3A%22Goudin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%5D%2C%22abstractNote%22%3A%22Vibrio%20cholerae%2C%20the%20causative%20agent%20of%20cholera%20epidemics%2C%20is%20a%20rod-shaped%20bacterium%20with%20a%20highly%20polarized%20cellular%20organization.%20It%20can%20survive%20harmful%20growth%20conditions%20by%20entering%20a%20non-proliferating%20spheroplast%20state%2C%20which%20involves%20loss%20of%20the%20cell%20envelope%20and%20polarity.%20How%20polarized%20rod%20organization%20cells%20are%20formed%20when%20the%20spheroplasts%20exit%20the%20non-proliferating%20state%20remains%20largely%20uncharacterized.%20To%20address%20this%20question%2C%20we%20investigated%20how%20L-arabinose-induced%20V.%20cholerae%20spheroplasts%20return%20to%20growth.%20We%20found%20that%20de%20novo%20morphogenesis%20started%20with%20the%20elimination%20of%20an%20excess%20of%20periplasm%2C%20which%20was%20immediately%20followed%20by%20cell%20elongation%20and%20the%20formation%20of%20cell%20branches%20with%20a%20diameter%20similar%20to%20that%20of%20normal%20V.%20cholerae%20cells.%20Periplasm%20elimination%20was%20driven%20by%20bifunctional%20peptidoglycan%20synthases%20involved%20in%20cell-wall%20maintenance%2C%20the%20aPBPs.%20Elongation%20and%20branching%20relied%20on%20the%20MreB-associated%20monofunctional%20peptidoglycan%20synthase%20PBP2.%20The%20cell%20division%20monofunctional%20peptidoglycan%20synthase%20FtsI%20was%20not%20involved%20in%20any%20of%20these%20processes.%20However%2C%20the%20FtsK%20cell%20division%20protein%20specifically%20targeted%20the%20sites%20of%20vesicle%20extrusion.%20Genetic%20material%20was%20amplified%20by%20synchronous%20waves%20of%20DNA%20replication%20as%20periplasmic%20elimination%20began.%20The%20HubP%20polarity%20factor%20targeted%20the%20tip%20of%20the%20branches%20as%20they%20began%20to%20form.%20However%2C%20HubP-mediated%20polarization%20was%20not%20involved%20in%20the%20efficiency%20of%20the%20recovery%20process.%20Finally%2C%20our%20results%20suggest%20that%20the%20positioning%20of%20HubP%20and%20the%20activities%20of%20the%20replication%20terminus%20organizer%20of%20the%20two%20V.%20cholerae%20chromosomes%2C%20MatP%2C%20are%20independent%20of%20cell%20division.%20Taken%20together%2C%20these%20results%20confirm%20the%20interest%20of%20L-arabinose-induced%20V.%20cholerae%20spheroplasts%20to%20study%20how%20cell%20shape%20is%20generated%20and%20shed%20light%20on%20the%20de%20novo%20establishment%20of%20the%20intracellular%20organization%20and%20cell%20polarization%20in%20V.%20cholerae.%22%2C%22date%22%3A%222023%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pone.0293276%22%2C%22ISSN%22%3A%221932-6203%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-10-27T07%3A52%3A00Z%22%7D%7D%2C%7B%22key%22%3A%2247EHFLMZ%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Camus%20et%20al.%22%2C%22parsedDate%22%3A%222023%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECamus%2C%20Adrien%2C%20Elena%20Espinosa%2C%20P%26%23xE9%3Bn%26%23xE9%3Blope%20Zapater%20Baras%2C%20Parul%20Singh%2C%20Nicole%20Quenech%26%23x2019%3BDu%2C%20Elise%20Vickridge%2C%20Mauro%20Modesti%2C%20Fran%26%23xE7%3Bois%20Xavier%20Barre%2C%20and%20Olivier%20Esp%26%23xE9%3Bli.%202023.%20%26%23x201C%3BThe%20SMC-like%20RecN%20Protein%20Is%20at%20the%20Crossroads%20of%20Several%20Genotoxic%20Stress%20Responses%20in%20Escherichia%20Coli.%26%23x201D%3B%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%2014%3A1146496.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2023.1146496%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2023.1146496%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20SMC-like%20RecN%20protein%20is%20at%20the%20crossroads%20of%20several%20genotoxic%20stress%20responses%20in%20Escherichia%20coli%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adrien%22%2C%22lastName%22%3A%22Camus%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%22%2C%22lastName%22%3A%22Espinosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P%5Cu00e9n%5Cu00e9lope%22%2C%22lastName%22%3A%22Zapater%20Baras%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Parul%22%2C%22lastName%22%3A%22Singh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicole%22%2C%22lastName%22%3A%22Quenech%27Du%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elise%22%2C%22lastName%22%3A%22Vickridge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mauro%22%2C%22lastName%22%3A%22Modesti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois%20Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Esp%5Cu00e9li%22%7D%5D%2C%22abstractNote%22%3A%22INTRODUCTION%3A%20DNA%20damage%20repair%20%28DDR%29%20is%20an%20essential%20process%20for%20living%20organisms%20and%20contributes%20to%20genome%20maintenance%20and%20evolution.%20DDR%20involves%20different%20pathways%20including%20Homologous%20recombination%20%28HR%29%2C%20Nucleotide%20Excision%20Repair%20%28NER%29%20and%20Base%20excision%20repair%20%28BER%29%20for%20example.%20The%20activity%20of%20each%20pathway%20is%20revealed%20with%20particular%20drug%20inducing%20lesions%2C%20but%20the%20repair%20of%20most%20DNA%20lesions%20depends%20on%20concomitant%20or%20subsequent%20action%20of%20the%20multiple%20pathways.%5CnMETHODS%3A%20In%20the%20present%20study%2C%20we%20used%20two%20genotoxic%20antibiotics%2C%20mitomycin%20C%20%28MMC%29%20and%20Bleomycin%20%28BLM%29%2C%20to%20decipher%20the%20interplays%20between%20these%20different%20pathways%20in%20E.%20coli.%20We%20combined%20genomic%20methods%20%28TIS%20and%20Hi-SC2%29%20and%20imaging%20assays%20with%20genetic%20dissections.%5CnRESULTS%3A%20We%20demonstrate%20that%20only%20a%20small%20set%20of%20DDR%20proteins%20are%20common%20to%20the%20repair%20of%20the%20lesions%20induced%20by%20these%20two%20drugs.%20Among%20them%2C%20RecN%2C%20an%20SMC-like%20protein%2C%20plays%20an%20important%20role%20by%20controlling%20sister%20chromatids%20dynamics%20and%20genome%20morphology%20at%20different%20steps%20of%20the%20repair%20processes.%20We%20further%20demonstrate%20that%20RecN%20influence%20on%20sister%20chromatids%20dynamics%20is%20not%20equivalent%20during%20the%20processing%20of%20the%20lesions%20induced%20by%20the%20two%20drugs.%20We%20observed%20that%20RecN%20activity%20and%20stability%20requires%20a%20pre-processing%20of%20the%20MMC-induced%20lesions%20by%20the%20NER%20but%20not%20for%20BLM-induced%20lesions.%5CnDISCUSSION%3A%20Those%20results%20show%20that%20RecN%20plays%20a%20major%20role%20in%20rescuing%20toxic%20intermediates%20generated%20by%20the%20BER%20pathway%20in%20addition%20to%20its%20well-known%20importance%20to%20the%20repair%20of%20double%20strand%20breaks%20by%20HR.%22%2C%22date%22%3A%222023%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2023.1146496%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-05-12T12%3A33%3A25Z%22%7D%7D%2C%7B%22key%22%3A%223GEPSZVJ%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Adam%20et%20al.%22%2C%22parsedDate%22%3A%222022-01-25%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAdam%2C%20Yazid%2C%20Pierre%20Brezellec%2C%20Elena%20Espinosa%2C%20Amelie%20Besombes%2C%20Delphine%20Naquin%2C%20Evelyne%20Paly%2C%20Christophe%20Possoz%2C%20Erwin%20van%20Dijk%2C%20Barre%20Francois-Xavier%2C%20and%20Ferat%20Jean-Luc.%202022.%20%26%23x201C%3BPlesiomonas%20Shigelloides%2C%20an%20Atypical%20Enterobacterales%20with%20a%20Vibrio-Related%20Secondary%20Chromosome.%26%23x201D%3B%20%3Ci%3EGenome%20Biology%20and%20Evolution%3C%5C%2Fi%3E%2C%20January%2C%20evac011.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgbe%5C%2Fevac011%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgbe%5C%2Fevac011%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Plesiomonas%20shigelloides%2C%20an%20Atypical%20Enterobacterales%20with%20a%20Vibrio-related%20secondary%20chromosome%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yazid%22%2C%22lastName%22%3A%22Adam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Brezellec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%22%2C%22lastName%22%3A%22Espinosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amelie%22%2C%22lastName%22%3A%22Besombes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Naquin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erwin%22%2C%22lastName%22%3A%22van%20Dijk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Barre%22%2C%22lastName%22%3A%22Francois-Xavier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ferat%22%2C%22lastName%22%3A%22Jean-Luc%22%7D%5D%2C%22abstractNote%22%3A%22About%2010%25%20of%20bacteria%20have%20a%20multi-chromosome%20genome%20with%20a%20primary%20replicon%20of%20bacterial%20origin%2C%20called%20the%20chromosome%2C%20and%20other%20replicons%20of%20plasmid%20origin%2C%20the%20chromids.%20Studies%20on%20multi-chromosome%20bacteria%20revealed%20potential%20points%20of%20coordination%20between%20the%20replication%5C%2Fsegregation%20of%20chromids%20and%20the%20progression%20of%20the%20cell%20cycle.%20For%20example%2C%20replication%20of%20the%20chromid%20of%20Vibrionales%20%28called%20Chr2%29%20is%20initiated%20upon%20duplication%20of%20a%20sequence%20carried%20by%20the%20primary%20chromosome%20%28called%20Chr1%29%2C%20in%20such%20a%20way%20that%20replication%20of%20both%20replicons%20is%20completed%20synchronously.%20Also%2C%20Chr2%20uses%20the%20Chr1%20as%20a%20scaffold%20for%20its%20partition%20in%20the%20daughter%20cells.%20How%20many%20of%20the%20features%20detected%20so%20far%20are%20required%20for%20the%20proper%20integration%20of%20a%20secondary%20chromosome%20in%20the%20cell%20cycle%3F%20How%20many%20more%20features%20remain%20to%20be%20discovered%3FWe%20hypothesized%20that%20critical%20features%20for%20the%20integration%20of%20the%20replication%5C%2Fsegregation%20of%20a%20given%20chromid%20within%20the%20cell%20cycle%20program%20would%20be%20conserved%20independently%20of%20the%20species%20in%20which%20the%20chromid%20has%20settled.%20Hence%2C%20we%20searched%20for%20a%20chromid%20related%20to%20that%20found%20in%20Vibrionales%20outside%20of%20this%20order.%20We%20identified%20one%20in%20Plesiomonas%20shigelloides%2C%20an%20aquatic%20and%20pathogenic%20enterobacterium%20that%20diverged%20early%20within%20the%20clade%20of%20Enterobacterales.Our%20results%20suggest%20that%20the%20chromids%20present%20in%20P.%20shigelloides%20and%20Vibrionales%20derive%20from%20a%20common%20ancestor.%20We%20initiated%20in%20sillico%20genomic%20and%20proteomic%20comparative%20analyses%20of%20P.%20shigelloides%2C%20Vibrionales%20and%20Enterobacterales%20that%20enabled%20us%20to%20establish%20a%20list%20of%20features%20likely%20involved%20in%20the%20maintenance%20of%20the%20chromid%20within%20the%20host%20cell%20cycle.%22%2C%22date%22%3A%222022-01-25%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fgbe%5C%2Fevac011%22%2C%22ISSN%22%3A%221759-6653%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgbe%5C%2Fevac011%22%2C%22collections%22%3A%5B%22GCG5UQZ2%22%5D%2C%22dateModified%22%3A%222023-12-14T15%3A41%3A31Z%22%7D%7D%2C%7B%22key%22%3A%22TX9C7TQK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Espinosa%20et%20al.%22%2C%22parsedDate%22%3A%222020-09-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEspinosa%2C%20Elena%2C%20Evelyne%20Paly%2C%20and%20Francois-Xavier%20Barre.%202020.%20%26%23x201C%3BHigh-Resolution%20Whole-Genome%20Analysis%20of%20Sister-Chromatid%20Contacts.%26%23x201D%3B%20%3Ci%3EMolecular%20Cell%3C%5C%2Fi%3E%2079%20%285%29%3A%20857%26%23x2013%3B69.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.molcel.2020.06.033%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.molcel.2020.06.033%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22High-Resolution%20Whole-Genome%20Analysis%20of%20Sister-Chromatid%20Contacts%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%22%2C%22lastName%22%3A%22Espinosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22Sister-chromatid%20cohesion%20describes%20the%20orderly%20association%20of%20newly%20replicated%20DNA%20molecules%20behind%20replication%20forks.%20It%20plays%20an%20essential%20role%20in%20the%20maintenance%20and%20faithful%20transmission%20of%20genetic%20information.%20Cohesion%20is%20created%20by%20DNA%20topological%20links%20and%20proteinaceous%20bridges%2C%20whose%20formation%20and%20deposition%20could%20be%20potentially%20affected%20by%20many%20processes.%20Current%20knowledge%20on%20cohesion%20has%20been%20mainly%20gained%20by%20fluorescence%20microscopy%20observation.%20However%2C%20the%20resolution%20limit%20of%20microscopy%20and%20the%20restricted%20number%20of%20genomic%20positions%20that%20can%20be%20simultaneously%20visualized%20considerably%20hampered%20progress.%20Here%2C%20we%20present%20a%20high-throughput%20methodology%20to%20monitor%20sister-chromatid%20contacts%20%28Hi-SC2%29.%20Using%20the%20multi-chromosomal%20Vibrio%20cholerae%20bacterium%20as%20a%20model%2C%20we%20show%20that%20Hi-SC2%20permits%20to%20monitor%20local%20variations%20in%20sister-chromatid%20cohesion%20at%20a%20high%20resolution%20over%20a%20whole%20genome.%22%2C%22date%22%3A%22SEP%203%202020%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.molcel.2020.06.033%22%2C%22ISSN%22%3A%221097-2765%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222021-03-04T15%3A21%3A28Z%22%7D%7D%2C%7B%22key%22%3A%22VM4HZAC9%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sinha%20et%20al.%22%2C%22parsedDate%22%3A%222020-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESinha%2C%20Anurag%20Kumar%2C%20Christophe%20Possoz%2C%20and%20David%20R.%20F.%20Leach.%202020.%20%26%23x201C%3BThe%20Roles%20of%20Bacterial%20DNA%20Double-Strand%20Break%20Repair%20Proteins%20in%20Chromosomal%20DNA%20Replication.%26%23x201D%3B%20%3Ci%3EFEMS%20Microbiology%20Reviews%3C%5C%2Fi%3E%2044%20%283%29%3A%20351%26%23x2013%3B68.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Ffemsre%5C%2Ffuaa009%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Ffemsre%5C%2Ffuaa009%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20roles%20of%20bacterial%20DNA%20double-strand%20break%20repair%20proteins%20in%20chromosomal%20DNA%20replication%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anurag%20Kumar%22%2C%22lastName%22%3A%22Sinha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20R.%20F.%22%2C%22lastName%22%3A%22Leach%22%7D%5D%2C%22abstractNote%22%3A%22It%20is%20well%20established%20that%20DNA%20double-strand%20break%20%28DSB%29%20repair%20is%20required%20to%20underpin%20chromosomal%20DNA%20replication.%20Because%20DNA%20replication%20forks%20are%20prone%20to%20breakage%2C%20faithful%20DSB%20repair%20and%20correct%20replication%20fork%20restart%20are%20critically%20important.%20Cells%2C%20where%20the%20proteins%20required%20for%20DSB%20repair%20are%20absent%20or%20altered%2C%20display%20characteristic%20disturbances%20to%20genome%20replication.%20In%20this%20review%2C%20we%20analyze%20how%20bacterial%20DNA%20replication%20is%20perturbed%20in%20DSB%20repair%20mutant%20strains%20and%20explore%20the%20consequences%20of%20these%20perturbations%20for%20bacterial%20chromosome%20segregation%20and%20cell%20viability.%20Importantly%2C%20we%20look%20at%20how%20DNA%20replication%20and%20DSB%20repair%20processes%20are%20implicated%20in%20the%20striking%20recent%20observations%20of%20DNA%20amplification%20and%20DNA%20loss%20in%20the%20chromosome%20terminus%20of%20various%20mutant%20Escherichia%20coli%20strains.%20We%20also%20address%20the%20mutant%20conditions%20required%20for%20the%20remarkable%20ability%20to%20copy%20the%20entire%20E.%20coli%20genome%2C%20and%20to%20maintain%20cell%20viability%2C%20even%20in%20the%20absence%20of%20replication%20initiation%20from%20oriC%2C%20the%20unique%20origin%20of%20DNA%20replication%20in%20wild%20type%20cells.%20Furthermore%2C%20we%20discuss%20the%20models%20that%20have%20been%20proposed%20to%20explain%20these%20phenomena%20and%20assess%20how%20these%20models%20fit%20with%20the%20observed%20data%2C%20provide%20new%20insights%2C%20and%20enhance%20our%20understanding%20of%20chromosomal%20replication%20and%20termination%20in%20bacteria.%22%2C%22date%22%3A%22MAY%2C%202020%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Ffemsre%5C%2Ffuaa009%22%2C%22ISSN%22%3A%221574-6976%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222020-09-16T09%3A11%3A30Z%22%7D%7D%2C%7B%22key%22%3A%22DAQPAJEC%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Midonet%20et%20al.%22%2C%22parsedDate%22%3A%222019-08-16%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMidonet%2C%20Caroline%2C%20Solange%20Miele%2C%20Evelyne%20Paly%2C%20Rapha%26%23xEB%3Bl%20Guerois%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202019.%20%26%23x201C%3BThe%20TLC%26%23x3A6%3B%20Satellite%20Phage%20Harbors%20a%20Xer%20Recombination%20Activation%20Factor.%26%23x201D%3B%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%20116%20%2837%29%3A%2018391%26%23x2013%3B96.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1902905116%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1902905116%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20TLC%5Cu03a6%20satellite%20phage%20harbors%20a%20Xer%20recombination%20activation%20factor%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Midonet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Solange%22%2C%22lastName%22%3A%22Miele%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Guerois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22The%20circular%20chromosomes%20of%20bacteria%20can%20be%20concatenated%20into%20dimers%20by%20homologous%20recombination.%20Dimers%20are%20solved%20by%20the%20addition%20of%20a%20cross-over%20at%20a%20specific%20chromosomal%20site%2C%20dif%2C%20by%202%20related%20tyrosine%20recombinases%2C%20XerC%20and%20XerD.%20Each%20enzyme%20catalyzes%20the%20exchange%20of%20a%20specific%20pair%20of%20strands.%20Some%20plasmids%20exploit%20the%20Xer%20machinery%20for%20concatemer%20resolution.%20Other%20mobile%20elements%20exploit%20it%20to%20integrate%20into%20the%20genome%20of%20their%20host.%20Chromosome%20dimer%20resolution%20is%20initiated%20by%20XerD.%20The%20reaction%20is%20under%20the%20control%20of%20a%20cell-division%20protein%2C%20FtsK%2C%20which%20activates%20XerD%20by%20a%20direct%20contact.%20Most%20mobile%20elements%20exploit%20FtsK-independent%20Xer%20recombination%20reactions%20initiated%20by%20XerC.%20The%20only%20notable%20exception%20is%20the%20toxin-linked%20cryptic%20satellite%20phage%20of%20Vibrio%20cholerae%2C%20TLC%5Cu03a6%2C%20which%20integrates%20into%20and%20excises%20from%20the%20dif%20site%20of%20the%20primary%20chromosome%20of%20its%20host%20by%20a%20reaction%20initiated%20by%20XerD.%20However%2C%20the%20reaction%20remains%20independent%20of%20FtsK.%20Here%2C%20we%20show%20that%20TLC%5Cu03a6%20carries%20a%20Xer%20recombination%20activation%20factor%2C%20XafT.%20We%20demonstrate%20in%20vitro%20that%20XafT%20activates%20XerD%20catalysis.%20Correspondingly%2C%20we%20found%20that%20XafT%20specifically%20interacts%20with%20XerD.%20We%20further%20show%20that%20integrative%20mobile%20elements%20exploiting%20Xer%20%28IMEXs%29%20encoding%20a%20XafT-like%20protein%20are%20widespread%20in%20gamma-%20and%20beta-proteobacteria%2C%20including%20human%2C%20animal%2C%20and%20plant%20pathogens.%22%2C%22date%22%3A%22Aug%2016%2C%202019%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1902905116%22%2C%22ISSN%22%3A%221091-6490%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%227EGDQ8LV%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222020-02-25T15%3A24%3A49Z%22%7D%7D%2C%7B%22key%22%3A%22LPYRRLIJ%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Galli%20et%20al.%22%2C%22parsedDate%22%3A%222019-06-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGalli%2C%20Elisa%2C%20Jean-Luc%20Ferat%2C%20Jean-Michel%20Desfontaines%2C%20Marie-Eve%20Val%2C%20Ole%20Skovgaard%2C%20Fran%26%23xE7%3Bois-Xavier%20Barre%2C%20and%20Christophe%20Possoz.%202019.%20%26%23x201C%3BReplication%20Termination%20without%20a%20Replication%20Fork%20Trap.%26%23x201D%3B%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%209%20%281%29%3A%208315.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-019-43795-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-019-43795-2%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Replication%20termination%20without%20a%20replication%20fork%20trap%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Michel%22%2C%22lastName%22%3A%22Desfontaines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Eve%22%2C%22lastName%22%3A%22Val%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ole%22%2C%22lastName%22%3A%22Skovgaard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%5D%2C%22abstractNote%22%3A%22Bacterial%20chromosomes%20harbour%20a%20unique%20origin%20of%20bidirectional%20replication%2C%20oriC.%20They%20are%20almost%20always%20circular%2C%20with%20replication%20terminating%20in%20a%20region%20diametrically%20opposite%20to%20oriC%2C%20the%20terminus.%20The%20oriC-terminus%20organisation%20is%20reflected%20by%20the%20orientation%20of%20the%20genes%20and%20by%20the%20disposition%20of%20DNA-binding%20protein%20motifs%20implicated%20in%20the%20coordination%20of%20chromosome%20replication%20and%20segregation%20with%20cell%20division.%20Correspondingly%2C%20the%20E.%20coli%20and%20B.%20subtilis%20model%20bacteria%20possess%20a%20replication%20fork%20trap%20system%2C%20Tus%5C%2Fter%20and%20RTP%5C%2Fter%2C%20respectively%2C%20which%20enforces%20replication%20termination%20in%20the%20terminus%20region.%20Here%2C%20we%20show%20that%20tus%20and%20rtp%20are%20restricted%20to%20four%20clades%20of%20bacteria%2C%20suggesting%20that%20tus%20was%20recently%20domesticated%20from%20a%20plasmid%20gene.%20We%20further%20demonstrate%20that%20there%20is%20no%20replication%20fork%20system%20in%20Vibrio%20cholerae%2C%20a%20bacterium%20closely%20related%20to%20E.%20coli.%20Marker%20frequency%20analysis%20showed%20that%20replication%20forks%20originating%20from%20ectopic%20origins%20were%20not%20blocked%20in%20the%20terminus%20region%20of%20either%20of%20the%20two%20V.%20cholerae%20chromosomes%2C%20but%20progressed%20normally%20until%20they%20encountered%20an%20opposite%20fork.%20As%20expected%2C%20termination%20synchrony%20of%20the%20two%20chromosomes%20is%20disrupted%20by%20these%20ectopic%20origins.%20Finally%2C%20we%20show%20that%20premature%20completion%20of%20the%20primary%20chromosome%20replication%20did%20not%20modify%20the%20choreography%20of%20segregation%20of%20its%20terminus%20region.%22%2C%22date%22%3A%22Jun%2005%2C%202019%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-019-43795-2%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222019-06-20T10%3A39%3A26Z%22%7D%7D%2C%7B%22key%22%3A%22W2UTV7HG%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Corcoles-Saez%20et%20al.%22%2C%22parsedDate%22%3A%222019-05-20%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECorcoles-Saez%2C%20Isaac%2C%20Jean-Luc%20Ferat%2C%20Michael%20Costanzo%2C%20Charles%20M.%20Boone%2C%20and%20Rita%20S.%20Cha.%202019.%20%26%23x201C%3BFunctional%20Link%20between%20Mitochondria%20and%20Rnr3%2C%20the%20Minor%20Catalytic%20Subunit%20of%20Yeast%20Ribonucleotide%20Reductase.%26%23x201D%3B%20%3Ci%3EMicrobial%20Cell%20%28Graz%2C%20Austria%29%3C%5C%2Fi%3E%206%20%286%29%3A%20286%26%23x2013%3B94.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.15698%5C%2Fmic2019.06.680%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.15698%5C%2Fmic2019.06.680%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Functional%20link%20between%20mitochondria%20and%20Rnr3%2C%20the%20minor%20catalytic%20subunit%20of%20yeast%20ribonucleotide%20reductase%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isaac%22%2C%22lastName%22%3A%22Corcoles-Saez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Costanzo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charles%20M.%22%2C%22lastName%22%3A%22Boone%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rita%20S.%22%2C%22lastName%22%3A%22Cha%22%7D%5D%2C%22abstractNote%22%3A%22Ribonucleotide%20reductase%20%28RNR%29%20is%20an%20essential%20holoenzyme%20required%20for%20de%20novo%20synthesis%20of%20dNTPs.%20The%20Saccharomyces%20cerevisiae%20genome%20encodes%20for%20two%20catalytic%20subunits%2C%20Rnr1%20and%20Rnr3.%20While%20Rnr1%20is%20required%20for%20DNA%20replication%20and%20DNA%20damage%20repair%2C%20the%20function%28s%29%20of%20Rnr3%20is%20unknown.%20Here%2C%20we%20show%20that%20carbon%20source%2C%20an%20essential%20nutrient%2C%20impacts%20Rnr1%20and%20Rnr3%20abundance%3A%20Non-fermentable%20carbon%20sources%20or%20limiting%20concentrations%20of%20glucose%20down%20regulate%20Rnr1%20and%20induce%20Rnr3%20expression.%20Oppositely%2C%20abundant%20glucose%20induces%20Rnr1%20expression%20and%20down%20regulates%20Rnr3.%20The%20carbon%20source%20dependent%20regulation%20of%20Rnr3%20is%20mediated%20by%20Mec1%2C%20the%20budding%20yeast%20ATM%5C%2FATR%20checkpoint%20response%20kinase.%20Unexpectedly%2C%20this%20regulation%20is%20independent%20of%20all%20currently%20known%20components%20of%20the%20Mec1%20DNA%20damage%20response%20network%2C%20including%20Rad53%2C%20Dun1%2C%20and%20Tel1%2C%20implicating%20a%20novel%20Mec1%20signalling%20axis.%20rnr3%5Cu0394%20leads%20to%20growth%20defects%20under%20respiratory%20conditions%20and%20rescues%20temperature%20sensitivity%20conferred%20by%20the%20absence%20of%20Tom6%2C%20a%20component%20of%20the%20mitochondrial%20TOM%20%28translocase%20of%20outer%20membrane%29%20complex%20responsible%20for%20mitochondrial%20protein%20import.%20Together%2C%20these%20results%20unveil%20involvement%20of%20Rnr3%20in%20mitochondrial%20functions%20and%20Mec1%20in%20mediating%20the%20carbon%20source%20dependent%20regulation%20of%20Rnr3.%22%2C%22date%22%3A%22May%2020%2C%202019%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.15698%5C%2Fmic2019.06.680%22%2C%22ISSN%22%3A%222311-2638%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222019-06-20T10%3A40%3A54Z%22%7D%7D%2C%7B%22key%22%3A%228BRNCMSH%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sinha%20et%20al.%22%2C%22parsedDate%22%3A%222018-03-09%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESinha%2C%20Anurag%20Kumar%2C%20Christophe%20Possoz%2C%20Adeline%20Durand%2C%20Jean-Michel%20Desfontaines%2C%20Fran%26%23xE7%3Bois-Xavier%20Barre%2C%20David%20R.%20F.%20Leach%2C%20and%20B%26%23xE9%3Bn%26%23xE9%3Bdicte%20Michel.%202018.%20%26%23x201C%3BBroken%20Replication%20Forks%20Trigger%20Heritable%20DNA%20Breaks%20in%20the%20Terminus%20of%20a%20Circular%20Chromosome.%26%23x201D%3B%20Edited%20by%20Nancy%20Maizels.%20%3Ci%3EPLOS%20Genetics%3C%5C%2Fi%3E%2014%20%283%29%3A%20e1007256.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1007256%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1007256%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Broken%20replication%20forks%20trigger%20heritable%20DNA%20breaks%20in%20the%20terminus%20of%20a%20circular%20chromosome%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anurag%20Kumar%22%2C%22lastName%22%3A%22Sinha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adeline%22%2C%22lastName%22%3A%22Durand%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Michel%22%2C%22lastName%22%3A%22Desfontaines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20R.%20F.%22%2C%22lastName%22%3A%22Leach%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B%5Cu00e9n%5Cu00e9dicte%22%2C%22lastName%22%3A%22Michel%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Nancy%22%2C%22lastName%22%3A%22Maizels%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222018-3-9%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pgen.1007256%22%2C%22ISSN%22%3A%221553-7404%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fdx.plos.org%5C%2F10.1371%5C%2Fjournal.pgen.1007256%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222019-04-17T13%3A21%3A47Z%22%7D%7D%2C%7B%22key%22%3A%22JRUSHUJ2%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sinha%20et%20al.%22%2C%22parsedDate%22%3A%222017-10-02%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESinha%2C%20Anurag%20Kumar%2C%20Adeline%20Durand%2C%20Jean-Michel%20Desfontaines%2C%20Ielyzaveta%20Iurchenko%2C%20H%26%23xE9%3Bl%26%23xE8%3Bne%20Auger%2C%20David%20R.%20F.%20Leach%2C%20Fran%26%23xE7%3Bois-Xavier%20Barre%2C%20and%20B%26%23xE9%3Bn%26%23xE9%3Bdicte%20Michel.%202017.%20%26%23x201C%3BDivision-Induced%20DNA%20Double%20Strand%20Breaks%20in%20the%20Chromosome%20Terminus%20Region%20of%20Escherichia%20Coli%20Lacking%20RecBCD%20DNA%20Repair%20Enzyme.%26%23x201D%3B%20%3Ci%3EPLoS%20Genetics%3C%5C%2Fi%3E%2013%20%2810%29%3A%20e1006895.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1006895%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1006895%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Division-induced%20DNA%20double%20strand%20breaks%20in%20the%20chromosome%20terminus%20region%20of%20Escherichia%20coli%20lacking%20RecBCD%20DNA%20repair%20enzyme%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anurag%20Kumar%22%2C%22lastName%22%3A%22Sinha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adeline%22%2C%22lastName%22%3A%22Durand%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Michel%22%2C%22lastName%22%3A%22Desfontaines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ielyzaveta%22%2C%22lastName%22%3A%22Iurchenko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H%5Cu00e9l%5Cu00e8ne%22%2C%22lastName%22%3A%22Auger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20R.%20F.%22%2C%22lastName%22%3A%22Leach%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B%5Cu00e9n%5Cu00e9dicte%22%2C%22lastName%22%3A%22Michel%22%7D%5D%2C%22abstractNote%22%3A%22Marker%20frequency%20analysis%20of%20the%20Escherichia%20coli%20recB%20mutant%20chromosome%20has%20revealed%20a%20deficit%20of%20DNA%20in%20a%20specific%20zone%20of%20the%20terminus%2C%20centred%20on%20the%20dif%5C%2FTerC%20region.%20Using%20fluorescence%20microscopy%20of%20a%20marked%20chromosomal%20site%2C%20we%20show%20that%20the%20dif%20region%20is%20lost%20after%20replication%20completion%2C%20at%20the%20time%20of%20cell%20division%2C%20in%20one%20daughter%20cell%20only%2C%20and%20that%20the%20phenomenon%20is%20transmitted%20to%20progeny.%20Analysis%20by%20marker%20frequency%20and%20microscopy%20shows%20that%20the%20position%20of%20DNA%20loss%20is%20not%20defined%20by%20the%20replication%20fork%20merging%20point%20since%20it%20still%20occurs%20in%20the%20dif%5C%2FTerC%20region%20when%20the%20replication%20fork%20trap%20is%20displaced%20in%20strains%20harbouring%20ectopic%20Ter%20sites.%20Terminus%20DNA%20loss%20in%20the%20recB%20mutant%20is%20also%20independent%20of%20dimer%20resolution%20by%20XerCD%20at%20dif%20and%20of%20Topo%20IV%20action%20close%20to%20dif.%20It%20occurs%20in%20the%20terminus%20region%2C%20at%20the%20point%20of%20inversion%20of%20the%20GC%20skew%2C%20which%20is%20also%20the%20point%20of%20convergence%20of%20specific%20sequence%20motifs%20like%20KOPS%20and%20Chi%20sites%2C%20regardless%20of%20whether%20the%20convergence%20of%20GC%20skew%20is%20at%20dif%20%28wild-type%29%20or%20a%20newly%20created%20sequence.%20In%20the%20absence%20of%20FtsK-driven%20DNA%20translocation%2C%20terminus%20DNA%20loss%20is%20less%20precisely%20targeted%20to%20the%20KOPS%20convergence%20sequence%2C%20but%20occurs%20at%20a%20similar%20frequency%20and%20follows%20the%20same%20pattern%20as%20in%20FtsK%2B%20cells.%20Importantly%2C%20using%20ftsIts%2C%20ftsAts%20division%20mutants%20and%20cephalexin%20treated%20cells%2C%20we%20show%20that%20DNA%20loss%20of%20the%20dif%20region%20in%20the%20recB%20mutant%20is%20decreased%20by%20the%20inactivation%20of%20cell%20division.%20We%20propose%20that%20it%20results%20from%20septum-induced%20chromosome%20breakage%2C%20and%20largely%20contributes%20to%20the%20low%20viability%20of%20the%20recB%20mutant.%22%2C%22date%22%3A%22Oct%2002%2C%202017%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pgen.1006895%22%2C%22ISSN%22%3A%221553-7404%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A42%3A21Z%22%7D%7D%2C%7B%22key%22%3A%22PK3ZRZFU%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Dalia%20et%20al.%22%2C%22parsedDate%22%3A%222017-07-07%22%2C%22numChildren%22%3A5%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDalia%2C%20Triana%20N.%2C%20Soo%20Hun%20Yoon%2C%20Elisa%20Galli%2C%20Francois-Xavier%20Barre%2C%20Christopher%20M.%20Waters%2C%20and%20Ankur%20B.%20Dalia.%202017.%20%26%23x201C%3BEnhancing%20Multiplex%20Genome%20Editing%20by%20Natural%20Transformation%20%28MuGENT%29%20via%20Inactivation%20of%20SsDNA%20Exonucleases.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2045%20%2812%29%3A%207527%26%23x2013%3B37.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkx496%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkx496%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Enhancing%20multiplex%20genome%20editing%20by%20natural%20transformation%20%28MuGENT%29%20via%20inactivation%20of%20ssDNA%20exonucleases%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Triana%20N.%22%2C%22lastName%22%3A%22Dalia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Soo%20Hun%22%2C%22lastName%22%3A%22Yoon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%20M.%22%2C%22lastName%22%3A%22Waters%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ankur%20B.%22%2C%22lastName%22%3A%22Dalia%22%7D%5D%2C%22abstractNote%22%3A%22Recently%2C%20we%20described%20a%20method%20for%20multiplex%20genome%20editing%20by%20natural%20transformation%20%28MuGENT%29.%20Mutant%20constructs%20for%20MuGENT%20require%20large%20arms%20of%20homology%20%28%26gt%3B2000%20bp%29%20surrounding%20each%20genome%20edit%2C%20which%20necessitates%20laborious%20in%20vitro%20DNA%20splicing.%20In%20Vibrio%20cholerae%2C%20we%20uncover%20that%20this%20requirement%20is%20due%20to%20cytoplasmic%20ssDNA%20exonucleases%2C%20which%20inhibit%20natural%20transformation.%20In%20ssDNA%20exonuclease%20mutants%2C%20one%20arm%20of%20homology%20can%20be%20reduced%20to%20as%20little%20as%2040%20bp%20while%20still%20promoting%20integration%20of%20genome%20edits%20at%20rates%20of%20%5Cu223c50%25%20without%20selection%20in%20cis.%20Consequently%2C%20editing%20constructs%20are%20generated%20in%20a%20single%20polymerase%20chain%20reaction%20where%20one%20homology%20arm%20is%20oligonucleotide%20encoded.%20To%20further%20enhance%20editing%20efficiencies%2C%20we%20also%20developed%20a%20strain%20for%20transient%20inactivation%20of%20the%20mismatch%20repair%20system.%20As%20a%20proof-of-concept%2C%20we%20used%20these%20advances%20to%20rapidly%20mutate%2010%20high-affinity%20binding%20sites%20for%20the%20nucleoid%20occlusion%20protein%20SlmA%20and%20generated%20a%20duodecuple%20mutant%20of%2012%20diguanylate%20cyclases%20in%20V.%20cholerae.%20Whole%20genome%20sequencing%20revealed%20little%20to%20no%20off-target%20mutations%20in%20these%20strains.%20Finally%2C%20we%20show%20that%20ssDNA%20exonucleases%20inhibit%20natural%20transformation%20in%20Acinetobacter%20baylyi.%20Thus%2C%20rational%20removal%20of%20ssDNA%20exonucleases%20may%20be%20broadly%20applicable%20for%20enhancing%20the%20efficacy%20and%20ease%20of%20MuGENT%20in%20diverse%20naturally%20transformable%20species.%22%2C%22date%22%3A%22July%207%2C%202017%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkx496%22%2C%22ISSN%22%3A%220305-1048%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkx496%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-03-11T14%3A09%3A58Z%22%7D%7D%2C%7B%22key%22%3A%22VUTDNTND%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Br%5Cu00e9zellec%20et%20al.%22%2C%22parsedDate%22%3A%222017-06-01%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBr%26%23xE9%3Bzellec%2C%20Pierre%2C%20Marie-Agn%26%23xE8%3Bs%20Petit%2C%20Sophie%20Pasek%2C%20Isabelle%20Vallet-Gely%2C%20Christophe%20Possoz%2C%20and%20Jean-Luc%20Ferat.%202017.%20%26%23x201C%3BDomestication%20of%20Lambda%20Phage%20Genes%20into%20a%20Putative%20Third%20Type%20of%20Replicative%20Helicase%20Matchmaker.%26%23x201D%3B%20%3Ci%3EGenome%20Biology%20and%20Evolution%3C%5C%2Fi%3E%209%20%286%29%3A%201561%26%23x2013%3B66.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgbe%5C%2Fevx111%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgbe%5C%2Fevx111%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Domestication%20of%20Lambda%20Phage%20Genes%20into%20a%20Putative%20Third%20Type%20of%20Replicative%20Helicase%20Matchmaker%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Br%5Cu00e9zellec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Agn%5Cu00e8s%22%2C%22lastName%22%3A%22Petit%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Pasek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabelle%22%2C%22lastName%22%3A%22Vallet-Gely%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%5D%2C%22abstractNote%22%3A%22At%20the%20onset%20of%20the%20initiation%20of%20chromosome%20replication%2C%20bacterial%20replicative%20helicases%20are%20recruited%20and%20loaded%20on%20the%20DnaA-oriC%20nucleoprotein%20platform%2C%20assisted%20by%20proteins%20like%20DnaC%5C%2FDnaI%20or%20DciA.%20Two%20orders%20of%20bacteria%20appear%2C%20however%2C%20to%20lack%20either%20of%20these%20factors%2C%20raising%20the%20question%20of%20the%20essentiality%20of%20these%20factors%20in%20bacteria.%20Through%20a%20phylogenomic%20approach%2C%20we%20identified%20a%20pair%20of%20genes%20that%20could%20have%20substituted%20for%20dciA.%20The%20two%20domesticated%20genes%20are%20specific%20of%20the%20dnaC%5C%2FdnaI-%20and%20dciA-lacking%20organisms%20and%20apparently%20domesticated%20from%20lambdoid%20phage%20genes.%20They%20derive%20from%20%5Cu03bbO%20and%20%5Cu03bbP%20and%20were%20renamed%20dopC%20and%20dopE%2C%20respectively.%20DopE%20is%20expected%20to%20bring%20the%20replicative%20helicase%20to%20the%20bacterial%20origin%20of%20replication%2C%20while%20DopC%20might%20assist%20DopE%20in%20this%20function.%20The%20confirmation%20of%20the%20implication%20of%20DopCE%20in%20the%20handling%20of%20the%20replicative%20helicase%20at%20the%20onset%20of%20replication%20in%20these%20organisms%20would%20generalize%20to%20all%20bacteria%20and%20therefore%20to%20all%20living%20organisms%20the%20need%20for%20specific%20factors%20dedicated%20to%20this%20function.%22%2C%22date%22%3A%22Jun%2001%2C%202017%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fgbe%5C%2Fevx111%22%2C%22ISSN%22%3A%221759-6653%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A42%3A32Z%22%7D%7D%2C%7B%22key%22%3A%22CJ5BJE44%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Galli%20et%20al.%22%2C%22parsedDate%22%3A%222017-03-30%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGalli%2C%20Elisa%2C%20Caroline%20Midonet%2C%20Evelyne%20Paly%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202017.%20%26%23x201C%3BFast%20Growth%20Conditions%20Uncouple%20the%20Final%20Stages%20of%20Chromosome%20Segregation%20and%20Cell%20Division%20in%20Escherichia%20Coli.%26%23x201D%3B%20Edited%20by%20William%20F.%20Burkholder.%20%3Ci%3EPLOS%20Genetics%3C%5C%2Fi%3E%2013%20%283%29%3A%20e1006702.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1006702%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1006702%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Fast%20growth%20conditions%20uncouple%20the%20final%20stages%20of%20chromosome%20segregation%20and%20cell%20division%20in%20Escherichia%20coli%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Midonet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22William%20F.%22%2C%22lastName%22%3A%22Burkholder%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222017-3-30%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pgen.1006702%22%2C%22ISSN%22%3A%221553-7404%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fdx.plos.org%5C%2F10.1371%5C%2Fjournal.pgen.1006702%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A44%3A41Z%22%7D%7D%2C%7B%22key%22%3A%223XZB5TTK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Galli%20et%20al.%22%2C%22parsedDate%22%3A%222017-03-16%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGalli%2C%20Elisa%2C%20Evelyne%20Paly%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202017.%20%26%23x201C%3BLate%20Assembly%20of%20the%20Vibrio%20Cholerae%20Cell%20Division%20Machinery%20Postpones%20Septation%20to%20the%20Last%2010%25%20of%20the%20Cell%20Cycle.%26%23x201D%3B%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%207%20%28March%29%3A44505.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fsrep44505%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fsrep44505%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Late%20assembly%20of%20the%20Vibrio%20cholerae%20cell%20division%20machinery%20postpones%20septation%20to%20the%20last%2010%25%20of%20the%20cell%20cycle%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222017-3-16%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fsrep44505%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fsrep44505%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-22T10%3A14%3A22Z%22%7D%7D%2C%7B%22key%22%3A%22PZK62RGQ%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Poidevin%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPoidevin%2C%20Micka%26%23xEB%3Bl%2C%20Elisa%20Galli%2C%20Yoshiharu%20Yamaichi%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202017.%20%26%23x201C%3BWGADseq%3A%20Whole%20Genome%20Affinity%20Determination%20of%20Protein-DNA%20Binding%20Sites.%26%23x201D%3B%20In%20%3Ci%3EThe%20Bacterial%20Nucleoid%3C%5C%2Fi%3E%2C%20edited%20by%20Olivier%20Esp%26%23xE9%3Bli%2C%201624%3A53%26%23x2013%3B60.%20Methods%20in%20Molecular%20Biology.%20New%20York%2C%20NY%3A%20Springer%20New%20York.%20https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2F978-1-4939-7098-8_5.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22bookSection%22%2C%22title%22%3A%22WGADseq%3A%20Whole%20Genome%20Affinity%20Determination%20of%20Protein-DNA%20Binding%20Sites%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Esp%5Cu00e9li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Micka%5Cu00ebl%22%2C%22lastName%22%3A%22Poidevin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoshiharu%22%2C%22lastName%22%3A%22Yamaichi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22bookTitle%22%3A%22The%20Bacterial%20Nucleoid%22%2C%22date%22%3A%222017%22%2C%22language%22%3A%22%22%2C%22ISBN%22%3A%22978-1-4939-7097-1%20978-1-4939-7098-8%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Flink.springer.com%5C%2F10.1007%5C%2F978-1-4939-7098-8_5%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222019-04-17T13%3A26%3A07Z%22%7D%7D%2C%7B%22key%22%3A%22TQQA89D2%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Espinosa%20et%20al.%22%2C%22parsedDate%22%3A%222017%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEspinosa%2C%20Elena%2C%20Fran%26%23xE7%3Bois-Xavier%20Barre%2C%20and%20Elisa%20Galli.%202017.%20%26%23x201C%3BCoordination%20between%20Replication%2C%20Segregation%20and%20Cell%20Division%20in%20Multi-Chromosomal%20Bacteria%3A%20Lessons%20from%20Vibrio%20Cholerae.%26%23x201D%3B%20%3Ci%3EInternational%20Microbiology.%20Official%20Journal%20of%20the%20Spanish%20Society%20for%20Microbiology%3C%5C%2Fi%3E%2C%20no.%2020%2C%20121%26%23x2013%3B29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.2436%5C%2F20.1501.01.293%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.2436%5C%2F20.1501.01.293%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Coordination%20between%20replication%2C%20segregation%20and%20cell%20division%20in%20multi-chromosomal%20bacteria%3A%20lessons%20from%20Vibrio%20cholerae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%22%2C%22lastName%22%3A%22Espinosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222017%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.2436%5C%2F20.1501.01.293%22%2C%22ISSN%22%3A%221618-1095%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Frevistes.iec.cat%5C%2Findex.php%5C%2FIM%5C%2Farticle%5C%2FviewFile%5C%2F144182%5C%2Fpdf_1352%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222019-04-17T13%3A27%3A00Z%22%7D%7D%2C%7B%22key%22%3A%22G2U6C3QK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Br%5Cu00e9zellec%20et%20al.%22%2C%22parsedDate%22%3A%222016-11-10%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBr%26%23xE9%3Bzellec%2C%20Pierre%2C%20Isabelle%20Vallet-Gely%2C%20Christophe%20Possoz%2C%20Sophie%20Quevillon-Cheruel%2C%20and%20Jean-Luc%20Ferat.%202016.%20%26%23x201C%3BDciA%20Is%20an%20Ancestral%20Replicative%20Helicase%20Operator%20Essential%20for%20Bacterial%20Replication%20Initiation.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%207%20%28November%29%3A13271.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fncomms13271%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fncomms13271%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22DciA%20is%20an%20ancestral%20replicative%20helicase%20operator%20essential%20for%20bacterial%20replication%20initiation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Br%5Cu00e9zellec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabelle%22%2C%22lastName%22%3A%22Vallet-Gely%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Quevillon-Cheruel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Ferat%22%7D%5D%2C%22abstractNote%22%3A%22Delivery%20of%20the%20replicative%20helicase%20onto%20DNA%20is%20an%20essential%20step%20in%20the%20initiation%20of%20replication.%20In%20bacteria%2C%20DnaC%20%28in%20Escherichia%20coli%29%20and%20DnaI%20%28in%20Bacillus%20subtilis%29%20are%20representative%20of%20the%20two%20known%20mechanisms%20that%20assist%20the%20replicative%20helicase%20at%20this%20stage.%20Here%2C%20we%20establish%20that%20these%20two%20strategies%20cannot%20be%20regarded%20as%20prototypical%20of%20the%20bacterial%20domain%20since%20dnaC%20and%20dnaI%20%28dna%5BCI%5D%29%20are%20present%20in%20only%20a%20few%20bacterial%20phyla.%20We%20show%20that%20dna%5BCI%5D%20was%20domesticated%20at%20least%20seven%20times%20through%20evolution%20in%20bacteria%20and%20at%20the%20expense%20of%20one%20gene%2C%20which%20we%20rename%20dciA%20%28dna%5BCI%5D%20antecedent%29%2C%20suggesting%20that%20DciA%20and%20Dna%5BCI%5D%20share%20a%20common%20function.%20We%20validate%20this%20hypothesis%20by%20establishing%20in%20Pseudomonas%20aeruginosa%20that%20DciA%20possesses%20the%20attributes%20of%20the%20replicative%20helicase-operating%20proteins%20associated%20with%20replication%20initiation.%22%2C%22date%22%3A%22Nov%2010%2C%202016%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fncomms13271%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A30%3A52Z%22%7D%7D%2C%7B%22key%22%3A%2242K7DH2D%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Midonet%20and%20Barre%22%2C%22parsedDate%22%3A%222016-07-26%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMidonet%2C%20Caroline%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202016.%20%26%23x201C%3BHow%20Xer-Exploiting%20Mobile%20Elements%20Overcome%20Cellular%20Control.%26%23x201D%3B%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America%3C%5C%2Fi%3E%20113%20%2830%29%3A%208343%26%23x2013%3B45.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1608539113%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1608539113%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22How%20Xer-exploiting%20mobile%20elements%20overcome%20cellular%20control%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Midonet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22Jul%2026%2C%202016%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1608539113%22%2C%22ISSN%22%3A%221091-6490%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-22T10%3A10%3A08Z%22%7D%7D%2C%7B%22key%22%3A%22ERMHTRD5%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Galli%20et%20al.%22%2C%22parsedDate%22%3A%222016-06-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGalli%2C%20Elisa%2C%20Micka%26%23xEB%3Bl%20Poidevin%2C%20Romain%20Le%20Bars%2C%20Jean-Michel%20Desfontaines%2C%20Leila%20Muresan%2C%20Evelyne%20Paly%2C%20Yoshiharu%20Yamaichi%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202016.%20%26%23x201C%3BCell%20Division%20Licensing%20in%20the%20Multi-Chromosomal%20Vibrio%20Cholerae%20Bacterium.%26%23x201D%3B%20%3Ci%3ENature%20Microbiology%3C%5C%2Fi%3E%201%20%289%29%3A%2016094.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnmicrobiol.2016.94%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnmicrobiol.2016.94%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cell%20division%20licensing%20in%20the%20multi-chromosomal%20Vibrio%20cholerae%20bacterium%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Galli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Micka%5Cu00ebl%22%2C%22lastName%22%3A%22Poidevin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Romain%22%2C%22lastName%22%3A%22Le%20Bars%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Michel%22%2C%22lastName%22%3A%22Desfontaines%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leila%22%2C%22lastName%22%3A%22Muresan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoshiharu%22%2C%22lastName%22%3A%22Yamaichi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222016-6-27%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fnmicrobiol.2016.94%22%2C%22ISSN%22%3A%222058-5276%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fnmicrobiol201694%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222019-04-17T13%3A28%3A54Z%22%7D%7D%2C%7B%22key%22%3A%22ZBHJPCZT%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mart%5Cu00ednez%20et%20al.%22%2C%22parsedDate%22%3A%222016-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMart%26%23xED%3Bnez%2C%20Eriel%2C%20Javier%20Campos-G%26%23xF3%3Bmez%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202016.%20%26%23x201C%3BCTX%26%23x3D5%3B%3A%20Exploring%20New%20Alternatives%20in%20Host%20Factor-Mediated%20Filamentous%20Phage%20Replications.%26%23x201D%3B%20%3Ci%3EBacteriophage%3C%5C%2Fi%3E%206%20%282%29%3A%20e1128512.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F21597081.2015.1128512%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1080%5C%2F21597081.2015.1128512%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CTX%5Cu03d5%3A%20Exploring%20new%20alternatives%20in%20host%20factor-mediated%20filamentous%20phage%20replications%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eriel%22%2C%22lastName%22%3A%22Mart%5Cu00ednez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Javier%22%2C%22lastName%22%3A%22Campos-G%5Cu00f3mez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22For%20a%20long%20time%20Ff%20phages%20from%20Escherichia%20coli%20provided%20the%20majority%20of%20the%20knowledge%20about%20the%20rolling%20circle%20replication%20mechanism%20of%20filamentous%20phages.%20Host%20factors%20involved%20in%20coliphages%20replication%20have%20been%20fully%20identified.%20Based%20on%20these%20studies%2C%20the%20function%20of%20Rep%20protein%20as%20the%20accessory%20helicase%20directly%20implicated%20in%20filamentous%20phage%20replication%20was%20considered%20a%20paradigm.%20We%20recently%20reported%20that%20the%20replication%20of%20some%20filamentous%20phages%20from%20Vibrio%20cholerae%2C%20including%20the%20cholera%20toxin%20phage%20CTX%5Cu03d5%2C%20depended%20on%20the%20accessory%20helicase%20UvrD%20instead%20of%20Rep.%20We%20also%20identified%20HU%20protein%20as%20one%20of%20the%20host%20factors%20involved%20in%20CTX%5Cu03d5%20and%20VGJ%5Cu03d5%20replication.%20The%20requirement%20of%20UvrD%20and%20HU%20for%20rolling%20circle%20replication%20was%20previously%20reported%20in%20some%20family%20of%20plasmids%20but%20had%20no%20precedent%20in%20filamentous%20phages.%20Here%2C%20we%20enrich%20the%20discussion%20of%20our%20results%20and%20present%20new%20preliminary%20data%20highlighting%20remarkable%20divergence%20in%20the%20lifestyle%20of%20filamentous%20phages.%22%2C%22date%22%3A%222016%20Apr-Jun%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1080%5C%2F21597081.2015.1128512%22%2C%22ISSN%22%3A%222159-7073%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A29%3A00Z%22%7D%7D%2C%7B%22key%22%3A%22URC9EJ75%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Val%20et%20al.%22%2C%22parsedDate%22%3A%222016-04%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVal%2C%20Marie-Eve%2C%20Martial%20Marbouty%2C%20Francisco%20de%20Lemos%20Martins%2C%20Sean%20P.%20Kennedy%2C%20Harry%20Kemble%2C%20Michael%20J.%20Bland%2C%20Christophe%20Possoz%2C%20Romain%20Koszul%2C%20Ole%20Skovgaard%2C%20and%20Didier%20Mazel.%202016.%20%26%23x201C%3BA%20Checkpoint%20Control%20Orchestrates%20the%20Replication%20of%20the%20Two%20Chromosomes%20of%20Vibrio%20Cholerae.%26%23x201D%3B%20%3Ci%3EScience%20Advances%3C%5C%2Fi%3E%202%20%284%29%3A%20e1501914.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.1501914%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.1501914%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20checkpoint%20control%20orchestrates%20the%20replication%20of%20the%20two%20chromosomes%20of%20Vibrio%20cholerae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Eve%22%2C%22lastName%22%3A%22Val%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martial%22%2C%22lastName%22%3A%22Marbouty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francisco%22%2C%22lastName%22%3A%22de%20Lemos%20Martins%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sean%20P.%22%2C%22lastName%22%3A%22Kennedy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harry%22%2C%22lastName%22%3A%22Kemble%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20J.%22%2C%22lastName%22%3A%22Bland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Possoz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Romain%22%2C%22lastName%22%3A%22Koszul%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ole%22%2C%22lastName%22%3A%22Skovgaard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Didier%22%2C%22lastName%22%3A%22Mazel%22%7D%5D%2C%22abstractNote%22%3A%22Bacteria%20with%20multiple%20chromosomes%20represent%20up%20to%2010%25%20of%20all%20bacterial%20species.%20Unlike%20eukaryotes%2C%20these%20bacteria%20use%20chromosome-specific%20initiators%20for%20their%20replication.%20In%20all%20cases%20investigated%2C%20the%20machineries%20for%20secondary%20chromosome%20replication%20initiation%20are%20of%20plasmid%20origin.%20One%20of%20the%20important%20differences%20between%20plasmids%20and%20chromosomes%20is%20that%20the%20latter%20replicate%20during%20a%20defined%20period%20of%20the%20cell%20cycle%2C%20ensuring%20a%20single%20round%20of%20replication%20per%20cell.%20Vibrio%20cholerae%20carries%20two%20circular%20chromosomes%2C%20Chr1%20and%20Chr2%2C%20which%20are%20replicated%20in%20a%20well-orchestrated%20manner%20with%20the%20cell%20cycle%20and%20coordinated%20in%20such%20a%20way%20that%20replication%20termination%20occurs%20at%20the%20same%20time.%20However%2C%20the%20mechanism%20coordinating%20this%20synchrony%20remains%20speculative.%20We%20investigated%20this%20mechanism%20and%20revealed%20that%20initiation%20of%20Chr2%20replication%20is%20triggered%20by%20the%20replication%20of%20a%20150-bp%20locus%20positioned%20on%20Chr1%2C%20called%20crtS.%20This%20crtS%20replication-mediated%20Chr2%20replication%20initiation%20mechanism%20explains%20how%20the%20two%20chromosomes%20communicate%20to%20coordinate%20their%20replication.%20Our%20study%20reveals%20a%20new%20checkpoint%20control%20mechanism%20in%20bacteria%2C%20and%20highlights%20possible%20functional%20interactions%20mediated%20by%20contacts%20between%20two%20chromosomes%2C%20an%20unprecedented%20observation%20in%20bacteria.%22%2C%22date%22%3A%22Apr%202016%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1126%5C%2Fsciadv.1501914%22%2C%22ISSN%22%3A%222375-2548%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A14%3A40Z%22%7D%7D%2C%7B%22key%22%3A%2254JRK99G%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mart%5Cu00ednez%20et%20al.%22%2C%22parsedDate%22%3A%222015-05%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMart%26%23xED%3Bnez%2C%20Eriel%2C%20Evelyne%20Paly%2C%20and%20Fran%26%23xE7%3Bois-Xavier%20Barre.%202015.%20%26%23x201C%3BCTX%26%23x3C6%3B%20Replication%20Depends%20on%20the%20Histone-Like%20HU%20Protein%20and%20the%20UvrD%20Helicase.%26%23x201D%3B%20%3Ci%3EPLoS%20Genetics%3C%5C%2Fi%3E%2011%20%285%29%3A%20e1005256.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1005256%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pgen.1005256%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CTX%5Cu03c6%20Replication%20Depends%20on%20the%20Histone-Like%20HU%20Protein%20and%20the%20UvrD%20Helicase%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eriel%22%2C%22lastName%22%3A%22Mart%5Cu00ednez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Evelyne%22%2C%22lastName%22%3A%22Paly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois-Xavier%22%2C%22lastName%22%3A%22Barre%22%7D%5D%2C%22abstractNote%22%3A%22The%20Vibrio%20cholerae%20bacterium%20is%20the%20agent%20of%20cholera.%20The%20capacity%20to%20produce%20the%20cholera%20toxin%2C%20which%20is%20responsible%20for%20the%20deadly%20diarrhea%20associated%20with%20cholera%20epidemics%2C%20is%20encoded%20in%20the%20genome%20of%20a%20filamentous%20phage%2C%20CTX%5Cu03c6.%20Rolling-circle%20replication%20%28RCR%29%20is%20central%20to%20the%20life%20cycle%20of%20CTX%5Cu03c6%20because%20amplification%20of%20the%20phage%20genome%20permits%20its%20efficient%20integration%20into%20the%20genome%20and%20its%20packaging%20into%20new%20viral%20particles.%20A%20single%20phage-encoded%20HUH%20endonuclease%20initiates%20RCR%20of%20the%20proto-typical%20filamentous%20phages%20of%20enterobacteriaceae%20by%20introducing%20a%20nick%20at%20a%20specific%20position%20of%20the%20double%20stranded%20DNA%20form%20of%20the%20phage%20genome.%20The%20rest%20of%20the%20process%20is%20driven%20by%20host%20factors%20that%20are%20either%20essential%20or%20crucial%20for%20the%20replication%20of%20the%20host%20genome%2C%20such%20as%20the%20Rep%20SF1%20helicase.%20In%20contrast%2C%20we%20show%20here%20that%20the%20histone-like%20HU%20protein%20of%20V.%20cholerae%20is%20necessary%20for%20the%20introduction%20of%20a%20nick%20by%20the%20HUH%20endonuclease%20of%20CTX%5Cu03c6.%20We%20further%20show%20that%20CTX%5Cu03c6%20RCR%20depends%20on%20a%20SF1%20helicase%20normally%20implicated%20in%20DNA%20repair%2C%20UvrD%2C%20rather%20than%20Rep.%20In%20addition%20to%20CTX%5Cu03c6%2C%20we%20show%20that%20VGJ%5Cu03c6%2C%20a%20representative%20member%20of%20a%20second%20family%20of%20vibrio%20integrative%20filamentous%20phages%2C%20requires%20UvrD%20and%20HU%20for%20RCR%20while%20TLC%5Cu03c6%2C%20a%20satellite%20phage%2C%20depends%20on%20Rep%20and%20is%20independent%20from%20HU.%22%2C%22date%22%3A%22May%202015%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pgen.1005256%22%2C%22ISSN%22%3A%221553-7404%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222018-03-15T12%3A28%3A57Z%22%7D%7D%5D%7D
Besombes, Amelie, Yazid Adam, Christophe Possoz, Ivan Junier, Francois-Xavier Barre, and Jean-Luc Ferat. 2024. “DciA Secures Bidirectional Replication Initiation in Vibrio Cholerae.” Nucleic Acids Research, September, gkae795. https://doi.org/10.1093/nar/gkae795.
Goudin, Anthony, Jean-Luc Ferat, Christophe Possoz, François-Xavier Barre, and Elisa Galli. 2023. “Recovery of Vibrio Cholerae Polarized Cellular Organization after Exit from a Non-Proliferating Spheroplast State.” PloS One 18 (10): e0293276. https://doi.org/10.1371/journal.pone.0293276.
Camus, Adrien, Elena Espinosa, Pénélope Zapater Baras, Parul Singh, Nicole Quenech’Du, Elise Vickridge, Mauro Modesti, François Xavier Barre, and Olivier Espéli. 2023. “The SMC-like RecN Protein Is at the Crossroads of Several Genotoxic Stress Responses in Escherichia Coli.” Frontiers in Microbiology 14:1146496. https://doi.org/10.3389/fmicb.2023.1146496.
Adam, Yazid, Pierre Brezellec, Elena Espinosa, Amelie Besombes, Delphine Naquin, Evelyne Paly, Christophe Possoz, Erwin van Dijk, Barre Francois-Xavier, and Ferat Jean-Luc. 2022. “Plesiomonas Shigelloides, an Atypical Enterobacterales with a Vibrio-Related Secondary Chromosome.” Genome Biology and Evolution, January, evac011. https://doi.org/10.1093/gbe/evac011.
Espinosa, Elena, Evelyne Paly, and Francois-Xavier Barre. 2020. “High-Resolution Whole-Genome Analysis of Sister-Chromatid Contacts.” Molecular Cell 79 (5): 857–69. https://doi.org/10.1016/j.molcel.2020.06.033.
Sinha, Anurag Kumar, Christophe Possoz, and David R. F. Leach. 2020. “The Roles of Bacterial DNA Double-Strand Break Repair Proteins in Chromosomal DNA Replication.” FEMS Microbiology Reviews 44 (3): 351–68. https://doi.org/10.1093/femsre/fuaa009.
Midonet, Caroline, Solange Miele, Evelyne Paly, Raphaël Guerois, and François-Xavier Barre. 2019. “The TLCΦ Satellite Phage Harbors a Xer Recombination Activation Factor.” Proceedings of the National Academy of Sciences of the United States of America 116 (37): 18391–96. https://doi.org/10.1073/pnas.1902905116.
Galli, Elisa, Jean-Luc Ferat, Jean-Michel Desfontaines, Marie-Eve Val, Ole Skovgaard, François-Xavier Barre, and Christophe Possoz. 2019. “Replication Termination without a Replication Fork Trap.” Scientific Reports 9 (1): 8315. https://doi.org/10.1038/s41598-019-43795-2.
Corcoles-Saez, Isaac, Jean-Luc Ferat, Michael Costanzo, Charles M. Boone, and Rita S. Cha. 2019. “Functional Link between Mitochondria and Rnr3, the Minor Catalytic Subunit of Yeast Ribonucleotide Reductase.” Microbial Cell (Graz, Austria) 6 (6): 286–94. https://doi.org/10.15698/mic2019.06.680.
Sinha, Anurag Kumar, Christophe Possoz, Adeline Durand, Jean-Michel Desfontaines, François-Xavier Barre, David R. F. Leach, and Bénédicte Michel. 2018. “Broken Replication Forks Trigger Heritable DNA Breaks in the Terminus of a Circular Chromosome.” Edited by Nancy Maizels. PLOS Genetics 14 (3): e1007256. https://doi.org/10.1371/journal.pgen.1007256.
Sinha, Anurag Kumar, Adeline Durand, Jean-Michel Desfontaines, Ielyzaveta Iurchenko, Hélène Auger, David R. F. Leach, François-Xavier Barre, and Bénédicte Michel. 2017. “Division-Induced DNA Double Strand Breaks in the Chromosome Terminus Region of Escherichia Coli Lacking RecBCD DNA Repair Enzyme.” PLoS Genetics 13 (10): e1006895. https://doi.org/10.1371/journal.pgen.1006895.
Dalia, Triana N., Soo Hun Yoon, Elisa Galli, Francois-Xavier Barre, Christopher M. Waters, and Ankur B. Dalia. 2017. “Enhancing Multiplex Genome Editing by Natural Transformation (MuGENT) via Inactivation of SsDNA Exonucleases.” Nucleic Acids Research 45 (12): 7527–37. https://doi.org/10.1093/nar/gkx496.
Brézellec, Pierre, Marie-Agnès Petit, Sophie Pasek, Isabelle Vallet-Gely, Christophe Possoz, and Jean-Luc Ferat. 2017. “Domestication of Lambda Phage Genes into a Putative Third Type of Replicative Helicase Matchmaker.” Genome Biology and Evolution 9 (6): 1561–66. https://doi.org/10.1093/gbe/evx111.
Galli, Elisa, Caroline Midonet, Evelyne Paly, and François-Xavier Barre. 2017. “Fast Growth Conditions Uncouple the Final Stages of Chromosome Segregation and Cell Division in Escherichia Coli.” Edited by William F. Burkholder. PLOS Genetics 13 (3): e1006702. https://doi.org/10.1371/journal.pgen.1006702.
Galli, Elisa, Evelyne Paly, and François-Xavier Barre. 2017. “Late Assembly of the Vibrio Cholerae Cell Division Machinery Postpones Septation to the Last 10% of the Cell Cycle.” Scientific Reports 7 (March):44505. https://doi.org/10.1038/srep44505.
Poidevin, Mickaël, Elisa Galli, Yoshiharu Yamaichi, and François-Xavier Barre. 2017. “WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites.” In The Bacterial Nucleoid, edited by Olivier Espéli, 1624:53–60. Methods in Molecular Biology. New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-7098-8_5.
Espinosa, Elena, François-Xavier Barre, and Elisa Galli. 2017. “Coordination between Replication, Segregation and Cell Division in Multi-Chromosomal Bacteria: Lessons from Vibrio Cholerae.” International Microbiology. Official Journal of the Spanish Society for Microbiology, no. 20, 121–29. https://doi.org/10.2436/20.1501.01.293.
Brézellec, Pierre, Isabelle Vallet-Gely, Christophe Possoz, Sophie Quevillon-Cheruel, and Jean-Luc Ferat. 2016. “DciA Is an Ancestral Replicative Helicase Operator Essential for Bacterial Replication Initiation.” Nature Communications 7 (November):13271. https://doi.org/10.1038/ncomms13271.
Midonet, Caroline, and François-Xavier Barre. 2016. “How Xer-Exploiting Mobile Elements Overcome Cellular Control.” Proceedings of the National Academy of Sciences of the United States of America 113 (30): 8343–45. https://doi.org/10.1073/pnas.1608539113.
Galli, Elisa, Mickaël Poidevin, Romain Le Bars, Jean-Michel Desfontaines, Leila Muresan, Evelyne Paly, Yoshiharu Yamaichi, and François-Xavier Barre. 2016. “Cell Division Licensing in the Multi-Chromosomal Vibrio Cholerae Bacterium.” Nature Microbiology 1 (9): 16094. https://doi.org/10.1038/nmicrobiol.2016.94.
Martínez, Eriel, Javier Campos-Gómez, and François-Xavier Barre. 2016. “CTXϕ: Exploring New Alternatives in Host Factor-Mediated Filamentous Phage Replications.” Bacteriophage 6 (2): e1128512. https://doi.org/10.1080/21597081.2015.1128512.
Val, Marie-Eve, Martial Marbouty, Francisco de Lemos Martins, Sean P. Kennedy, Harry Kemble, Michael J. Bland, Christophe Possoz, Romain Koszul, Ole Skovgaard, and Didier Mazel. 2016. “A Checkpoint Control Orchestrates the Replication of the Two Chromosomes of Vibrio Cholerae.” Science Advances 2 (4): e1501914. https://doi.org/10.1126/sciadv.1501914.
Martínez, Eriel, Evelyne Paly, and François-Xavier Barre. 2015. “CTXφ Replication Depends on the Histone-Like HU Protein and the UvrD Helicase.” PLoS Genetics 11 (5): e1005256. https://doi.org/10.1371/journal.pgen.1005256.