Membrane Proteins
and Membrane Systems Laboratory

Catalina-Hernández, Èric, Mario López-Martín, David Masnou-Sánchez, Marco Martins, Victor A. Lorenz-Fonfria, Francesc Jiménez-Altayó, Ute A. Hellmich, et al. 2024. “Experimental and Computational Biophysics to Identify Vasodilator Drugs Targeted at TRPV2 Using Agonists Based on the Probenecid Scaffold.” Computational and Structural Biotechnology Journal 23 (December):473–82. https://doi.org/10.1016/j.csbj.2023.12.028.

Sebinelli, Heitor Gobbi, Camille Syska, Alenka Čopič, and Guillaume Lenoir. 2024. “Established and Emerging Players in Phospholipid Scrambling: A Structural Perspective.” Biochimie, September, S0300-9084(24)00218-9. https://doi.org/10.1016/j.biochi.2024.09.008.

Herrera, Sara Abad, Bo Højen Justesen, Thibaud Dieudonné, Cédric Montigny, Poul Nissen, Guillaume Lenoir, and Thomas Günther Pomorski. 2023. “Direct Evidence of Lipid Transport by the Drs2-Cdc50 Flippase upon Truncation of Its Terminal Regions.” Protein Science: A Publication of the Protein Society, December, e4855. https://doi.org/10.1002/pro.4855.

Loconte, Luisa, Davinia Arguedas, Rojbin El, Alix Zhou, Anna Chipont, Lea Guyonnet, Coralie Guerin, et al. 2023. “Detection of the Interactions of Tumour Derived Extracellular Vesicles with Immune Cells Is Dependent on EV-Labelling Methods.” Journal of Extracellular Vesicles 12 (12): e12384. https://doi.org/10.1002/jev2.12384.

Dieudonné, Thibaud, Felix Kümmerer, Michelle Juknaviciute Laursen, Charlott Stock, Rasmus Kock Flygaard, Syma Khalid, Guillaume Lenoir, Joseph A. Lyons, Kresten Lindorff-Larsen, and Poul Nissen. 2023. “Activation and Substrate Specificity of the Human P4-ATPase ATP8B1.” Nature Communications 14 (1): 7492. https://doi.org/10.1038/s41467-023-42828-9.

Čopič, Alenka, Thibaud Dieudonné, and Guillaume Lenoir. 2023. “Phosphatidylserine Transport in Cell Life and Death.” Current Opinion in Cell Biology 83 (July):102192. https://doi.org/10.1016/j.ceb.2023.102192.

Tabares, Leandro C., Davis T. Daniel, José Luis Vázquez-Ibar, Cyrille Kouklovsky, Valérie Alezra, and Sun Un. 2023. “Using the Noncanonical Metallo-Amino Acid [Cu(II)(2,2’-Bipyridin-5-Yl)]-Alanine to Study the Structures of Proteins.” The Journal of Physical Chemistry Letters, March, 3368–75. https://doi.org/10.1021/acs.jpclett.3c00196.

Roux, Michel, François-Xavier Legrand, Abed Bil, Véronique Bonnet, and Florence Djedaini-Pilard. 2023. “Fragmentation of DMPC Membranes by a Wedge-Shaped Amphiphilic Cyclodextrin into Bicellar-like Aggregates.” The Journal of Physical Chemistry. B, March. https://doi.org/10.1021/acs.jpcb.2c07331.

Dieudonné, Thibaud, Christine Jaxel, Maylis Lejeune, Guillaume Lenoir, and Cédric Montigny. 2023. “Expression in Saccharomyces Cerevisiae and Purification of a Human Phospholipid Flippase.” Methods in Molecular Biology (Clifton, N.J.) 2652:231–46. https://doi.org/10.1007/978-1-0716-3147-8_13.

Flourieusse, A., P. Bourgeois, E. Schenckbecher, J. Palvair, D. Legrand, C. Labbé, T. Bescond, et al. 2022. “Formation of Intracellular Vesicles within the Gram+ Lactococcus Lactis Induced by the Overexpression of Caveolin-1β.” Microbial Cell Factories 21 (1): 239. https://doi.org/10.1186/s12934-022-01944-9.

Perrot, Nahuel, Delphine Dessaux, Anthony Rignani, Cynthia Gillet, Stéphane Orlowski, Nadège Jamin, Manuel Garrigos, and Christine Jaxel. 2022. “Caveolin-1β Promotes the Production of Active Human Microsomal Glutathione S-Transferase in Induced Intracellular Vesicles in Spodoptera Frugiperda 21 Insect Cells.” Biochimica et Biophysica Acta (BBA) - Biomembranes 1864 (8): 183922. https://doi.org/10.1016/j.bbamem.2022.183922.

López-Martín, Mario, Pedro Renault, Jesus Giraldo, José Luis Vázquez-Ibar, and Alex Perálvarez-Marín. 2022. “In Silico Assessment of the Lipid Fingerprint Signature of ATP2, the Essential P4-ATPase of Malaria Parasites.” Membranes 12 (7): 702. https://doi.org/10.3390/membranes12070702.

Gobet, Alexia, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, Stéphane Orlowski, Nadège Jamin, Pierre Falson, and Vincent Chaptal. 2022. “Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia Coli.” In Heterologous Expression of Membrane Proteins: Methods and Protocols, edited by Isabelle Mus-Veteau, 79–89. Methods in Molecular Biology. New York, NY: Springer US. https://doi.org/10.1007/978-1-0716-2368-8_5.

Dieudonné, Thibaud, Sara Abad Herrera, Michelle Juknaviciute Laursen, Maylis Lejeune, Charlott Stock, Kahina Slimani, Christine Jaxel, et al. 2022. “Autoinhibition and Regulation by Phosphoinositides of ATP8B1, a Human Lipid Flippase Associated with Intrahepatic Cholestatic Disorders.” Edited by Merritt Maduke. ELife 11 (April):e75272. https://doi.org/10.7554/eLife.75272.

Orlowski, Stéphane, Jean-Jacques Mourad, Antonio Gallo, and Eric Bruckert. 2021. “Coronaviruses, Cholesterol and Statins: Involvement and Application for Covid-19.” Biochimie 189 (October):51–64. https://doi.org/10.1016/j.biochi.2021.06.005.

Timcenko, Milena, Thibaud Dieudonné, Cédric Montigny, Thomas Boesen, Joseph A. Lyons, Guillaume Lenoir, and Poul Nissen. 2021. “Structural Basis of Substrate-Independent Phosphorylation in a P4-ATPase Lipid Flippase.” Journal of Molecular Biology, May, 167062. https://doi.org/10.1016/j.jmb.2021.167062.

Barbot, Thomas, Veronica Beswick, Cédric Montigny, Éric Quiniou, Nadège Jamin, and Liliane Mouawad. 2021. “Deciphering the Mechanism of Inhibition of SERCA1a by Sarcolipin Using Molecular Simulations.” Frontiers in Molecular Biosciences 7 (February):606254. https://doi.org/10.3389/fmolb.2020.606254.

Macherel, David, Francis Haraux, Hervé Guillou, and Olivier Bourgeois. 2021. “The Conundrum of Hot Mitochondria.” Biochimica et Biophysica Acta (BBA) - Bioenergetics 1862 (2): 148348. https://doi.org/10.1016/j.bbabio.2020.148348.

Lamy, Anaïs, Ewerton Macarini-Bruzaferro, Thibaud Dieudonné, Alex Perálvarez-Marín, Guillaume Lenoir, Cédric Montigny, Marc le Maire, and José Luis Vázquez-Ibar. 2021. “ATP2, The Essential P4-ATPase of Malaria Parasites, Catalyzes Lipid-Stimulated ATP Hydrolysis in Complex with a Cdc50 β-Subunit.” Emerging Microbes & Infections 10 (1): 132–47. https://doi.org/10.1080/22221751.2020.1870413.

Montigny, Cédric, Dong Liang Huang, Veronica Beswick, Thomas Barbot, Christine Jaxel, Marc le Maire, Ji-Shen Zheng, and Nadège Jamin. 2021. “Sarcolipin Alters SERCA1a Interdomain Communication by Impairing Binding of Both Calcium and ATP.” Scientific Reports 11 (1): 1641. https://doi.org/10.1038/s41598-021-81061-6.

Lenoir, Guillaume, Juan Martín D’Ambrosio, Thibaud Dieudonné, and Alenka Čopič. 2021. “Transport Pathways That Contribute to the Cellular Distribution of Phosphatidylserine.” Frontiers in Cell and Developmental Biology 9:737907. https://doi.org/10.3389/fcell.2021.737907.

Geurts, Maxwell M. G., Johannes D. Clausen, Bertrand Arnou, Cédric Montigny, Guillaume Lenoir, Robin A. Corey, Christine Jaxel, et al. 2020. “The SERCA Residue Glu340 Mediates Interdomain Communication That Guides Ca2+ Transport.” Proceedings of the National Academy of Sciences 117 (49): 31114–22. https://doi.org/10.1073/pnas.2014896117.

Rucheton, Benoit, Claude Jardel, Sandrine Filaut, Maria del Mar Amador, Thierry Maisonobe, Isabelle Serre, Norma Beatriz Romero, Sarah Leonard-Louis, Francis Haraux, and Anne Lombès. 2020. “Homoplasmic Deleterious MT-ATP6/8 Mutations in Adult Patients.” Mitochondrion 55 (November):64–77. https://doi.org/10.1016/j.mito.2020.08.004.

Lyons, Joseph A., Milena Timcenko, Thibaud Dieudonne, Guillaume Lenoir, and Poul Nissen. 2020. “ATPases: How an Old Dog Learnt New Tricks - Structure and Mechanism of Lipid Flippases.” Current Opinion in Structural Biology 63 (August):65–73. https://doi.org/10.1016/j.sbi.2020.04.001.

Maréchal, Amandine, Jing-Yang Xu, Naho Genko, Andrew M. Hartley, Francis Haraux, Brigitte Meunier, and Peter R. Rich. 2020. “A Common Coupling Mechanism for A-Type Heme-Copper Oxidases from Bacteria to Mitochondria.” Proceedings of the National Academy of Sciences of the United States of America 117 (17): 9349–55. https://doi.org/10.1073/pnas.2001572117.

Huang, Dong-Liang, Cédric Montigny, Yong Zheng, Veronica Beswick, Ying Li, Xiu-Xiu Cao, Thomas Barbot, et al. 2019. “Chemical Synthesis of Native S-Palmitoylated Membrane Proteins through Removable-Backbone-Modification-Assisted Ser/Thr Ligation.” Angewandte Chemie International Edition 59 (13): 5178–84. https://doi.org/https://doi.org/10.1002/anie.201914836.

Roux, Michel, Véronique Bonnet, and Florence Djedaïni-Pilard. 2019. “Ordering of Saturated and Unsaturated Lipid Membranes near Their Phase Transitions Induced by an Amphiphilic Cyclodextrin and Cholesterol.” Langmuir: The ACS Journal of Surfaces and Colloids 35 (44): 14376–87. https://doi.org/10.1021/acs.langmuir.9b02082.

Champeil, Philippe, Béatrice de Foresta, Martin Picard, Carole Gauron, Dominique Georgin, Marc le Maire, Jesper V. Møller, Guillaume Lenoir, and Cédric Montigny. 2019. “Interaction of Detergents with Biological Membranes: Comparison of Fluorescence Assays with Filtration Protocols and Implications for the Rates of Detergent Association, Dissociation and Flip-Flop.” PloS One 14 (10): e0222932. https://doi.org/10.1371/journal.pone.0222932.

Timcenko, Milena, Joseph A. Lyons, Dovile Januliene, Jakob J. Ulstrup, Thibaud Dieudonné, Cédric Montigny, Miriam-Rose Ash, et al. 2019. “Structure and Autoregulation of a P4-ATPase Lipid Flippase.” Nature 571 (June):366–70. https://doi.org/10.1038/s41586-019-1344-7.

Donate-Macian, Pau, Elena Alvarez-Marimon, Francesc Sepulcre, Jose Luis Vazquez-Ibar, and Alex Peralvarez-Marin. 2019. “The Membrane Proximal Domain of TRPV1 and TRPV2 Channels Mediates Protein-Protein Interactions and Lipid Binding In Vitro.” International Journal of Molecular Sciences 20 (3): 682. https://doi.org/10.3390/ijms20030682.

Sauvage, Félix, François-Xavier Legrand, Michel Roux, Ivan Rajkovic, Thomas M. Weiss, Zoltán Varga, Luc Augis, et al. 2018. “Interaction of Dequalinium Chloride with Phosphatidylcholine Bilayers: A Biophysical Study with Consequences on the Development of Lipid-Based Mitochondrial Nanomedicines.” Journal of Colloid and Interface Science 537 (November):704–15. https://doi.org/10.1016/j.jcis.2018.11.059.

Jamin, Nadege, Manuel Garrigos, Christine Jaxel, Annie Frelet-Barrand, and Stephane Orlowski. 2018. “Ectopic Neo-Formed Intracellular Membranes in Escherichia Coli: A Response to Membrane Protein-Induced Stress Involving Membrane Curvature and Domains.” Biomolecules 8 (3): 88. https://doi.org/10.3390/biom8030088.

Lenoir, Guillaume, Thibaud Dieudonné, Anaïs Lamy, Maylis Lejeune, José-Luis Vazquez-Ibar, and Cédric Montigny. 2018. “Screening of Detergents for Stabilization of Functional Membrane Proteins.” Current Protocols in Protein Science 93 (1): e59. https://doi.org/10.1002/cpps.59.

David, Marion, Chantal Lebrun, Thomas Duguet, Franck Talmont, Robin Beech, Stéphane Orlowski, François André, Roger K. Prichard, and Anne Lespine. 2018. “Structural Model, Functional Modulation by Ivermectin and Tissue Localization of Haemonchus Contortus P-Glycoprotein-13.” International Journal for Parasitology. Drugs and Drug Resistance 8 (1): 145–57. https://doi.org/10.1016/j.ijpddr.2018.02.001.

Acuña, Alonso M., Claire Lemaire, Rienk van Grondelle, Bruno Robert, and Ivo H. M. van Stokkum. 2018. “Energy Transfer and Trapping in Synechococcus WH 7803.” Photosynthesis Research 135 (1–3): 115–24. https://doi.org/10.1007/s11120-017-0451-2.

Muzzopappa, Fernando, Adjélé Wilson, Vinosa Yogarajah, Sandrine Cot, François Perreau, Cédric Montigny, Céline Bourcier de Carbon, and Diana Kirilovsky. 2017. “The Paralogs to the C-Terminal Domain of the Cyanobacterial OCP Are Carotenoid Donors to HCPs.” Plant Physiology 175 (3): 1283–1303. https://doi.org/10.1104/pp.17.01040.

Azouaoui, Hassina, Cédric Montigny, Thibaud Dieudonné, Philippe Champeil, Aurore Jacquot, José Luis Vázquez-Ibar, Pierre Le Maréchal, et al. 2017. “A High and Phosphatidylinositol-4-Phosphate (PI4P)-Dependent ATPase Activity for the Drs2p/Cdc50p Flippase after Removal of Its N- and C-Terminal Extensions.” Journal of Biological Chemistry 292 (19): jbc.M116.751487. https://doi.org/10.1074/jbc.M116.751487.

Chaptal, Vincent, Frédéric Delolme, Arnaud Kilburg, Sandrine Magnard, Cédric Montigny, Martin Picard, Charlène Prier, et al. 2017. “Quantification of Detergents Complexed with Membrane Proteins.” Scientific Reports 7 (February):41751. https://doi.org/10.1038/srep41751.

Montigny, Cédric, Thibaud Dieudonné, Stéphane Orlowski, José Luis Vázquez-Ibar, Carole Gauron, Dominique Georgin, Sten Lund, et al. 2017. “Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles: Brominated Phospholipids as Tools to Follow Its Kinetics.” Edited by Hendrik W. van Veen. PLOS ONE 12 (1): e0170481. https://doi.org/10.1371/journal.pone.0170481.

Errasti-Murugarren, Ekaitz, Arturo Rodríguez-Banqueri, and José Luis Vázquez-Ibar. 2017. “Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. Coli: A Tool to Engineer Stability in a LAT Transporter.” In Heterologous Gene Expression in E.Coli, edited by Nicola A. Burgess-Brown, 1586:181–95. Methods in Molecular Biology. New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-6887-9_11.

Negroni, Luc, Michel Zivy, and Claire Lemaire. 2017. “Mass Spectrometry of Mitochondrial Membrane Protein Complexes.” In Membrane Protein Structure and Function Characterization, edited by Jean-Jacques Lacapere, 1635:233–46. Methods in Molecular Biology. New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-7151-0_12.

Gaibelet, Gérald, François Tercé, Sophie Allart, Chantal Lebrun, Xavier Collet, Nadège Jamin, and Stéphane Orlowski. 2017. “Fluorescent Probes for Detecting Cholesterol-Rich Ordered Membrane Microdomains: Entangled Relationships between Structural Analogies in the Membrane and Functional Homologies in the Cell.” AIMS Biophysics 4 (1): 121–51. https://doi.org/10.3934/biophy.2017.1.121.

Champeil, Philippe, Stéphane Orlowski, Simon Babin, Sten Lund, Marc le Maire, Jesper Møller, Guillaume Lenoir, and Cédric Montigny. 2016. “A Robust Method to Screen Detergents for Membrane Protein Stabilization, Revisited.” Analytical Biochemistry 511 (October):31–35. https://doi.org/10.1016/j.ab.2016.07.017.

Montigny, Cédric, Joseph Lyons, Philippe Champeil, Poul Nissen, and Guillaume Lenoir. 2016. “On the Molecular Mechanism of Flippase- and Scramblase-Mediated Phospholipid Transport.” Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1861 (8): 767–83. https://doi.org/10.1016/j.bbalip.2015.12.020.

David-Bosne, Stéphanie, Michael Voldsgaard Clausen, Hanne Poulsen, Jesper Vuust Møller, Poul Nissen, and Marc le Maire. 2016. “Erratum: Reappraising the Effects of Artemisinin on the ATPase Activity of PfATP6 and SERCA1a E255L Expressed in Xenopus Laevis Oocytes.” Nature Structural & Molecular Biology 23 (4): 358–358. https://doi.org/10.1038/nsmb0416-358a.

Rodríguez-Banqueri, Arturo, Ekaitz Errasti-Murugarren, Paola Bartoccioni, Lukasz Kowalczyk, Alex Perálvarez-Marín, Manuel Palacín, and José Luis Vázquez-Ibar. 2016. “Stabilization of a Prokaryotic LAT Transporter by Random Mutagenesis.” The Journal of General Physiology 147 (4): 353–68. https://doi.org/10.1085/jgp.201511510.

David-Bosne, Stéphanie, Michael Voldsgaard Clausen, Hanne Poulsen, Jesper Vuust Møller, Poul Nissen, and Marc le Maire. 2016. “Reappraising the Effects of Artemisinin on the ATPase Activity of PfATP6 and SERCA1a E255L Expressed in Xenopus Laevis Oocytes.” Nature Structural & Molecular Biology 23 (1): 1–2. https://doi.org/10.1038/nsmb.3156.

David, Marion A., Stéphane Orlowski, Roger K. Prichard, Shaima Hashem, François André, and Anne Lespine. 2016. “In Silico Analysis of the Binding of Anthelmintics to Caenorhabditis Elegans P-Glycoprotein 1.” International Journal for Parasitology: Drugs and Drug Resistance 6 (3): 299–313. https://doi.org/10.1016/j.ijpddr.2016.09.001.

Bakari, Sana, Mehdi Lembrouk, Laura Sourd, Fares Ousalem, François André, Stéphane Orlowski, Marcel Delaforge, and Annie Frelet-Barrand. 2016. “Lactococcus Lactis Is an Efficient Expression System for Mammalian Membrane Proteins Involved in Liver Detoxification, CYP3A4, and MGST1.” Molecular Biotechnology 58 (4): 299–310. https://doi.org/10.1007/s12033-016-9928-z.

Le Breton, Nolwenn, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, and Valérie Belle. 2016. “Dimerization Interface and Dynamic Properties of Yeast IF1 Revealed by Site-Directed Spin Labeling EPR Spectroscopy.” Biochimica et Biophysica Acta (BBA) - Bioenergetics 1857 (1): 89–97. https://doi.org/10.1016/j.bbabio.2015.10.010.