RNA-binding proteins in gene expression
and cell differentiation
Publications
3888256
GEXDIF
chicago-author-date
50
date
desc
year
14234
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-7aa727498c4937dfa41eb2ddc3f98bbe%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22KUU2GNXR%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Penzo%20et%20al.%22%2C%22parsedDate%22%3A%222023-09-20%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPenzo%2C%20Arianna%2C%20Marion%20Dubarry%2C%20Cl%26%23xE9%3Bmentine%20Brocas%2C%20Myriam%20Zheng%2C%20Rapha%26%23xEB%3Bl%20M.%20Mangione%2C%20Mathieu%20Rougemaille%2C%20Coralie%20Goncalves%2C%20et%20al.%202023.%20%26%23x201C%3BA%20R-Loop%20Sensing%20Pathway%20Mediates%20the%20Relocation%20of%20Transcribed%20Genes%20to%20Nuclear%20Pore%20Complexes.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2014%20%281%29%3A%205606.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-41345-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-41345-z%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20R-loop%20sensing%20pathway%20mediates%20the%20relocation%20of%20transcribed%20genes%20to%20nuclear%20pore%20complexes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arianna%22%2C%22lastName%22%3A%22Penzo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Dubarry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cl%5Cu00e9mentine%22%2C%22lastName%22%3A%22Brocas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Zheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%20M.%22%2C%22lastName%22%3A%22Mangione%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Coralie%22%2C%22lastName%22%3A%22Goncalves%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Oph%5Cu00e9lie%22%2C%22lastName%22%3A%22Lautier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Domenico%22%2C%22lastName%22%3A%22Libri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-No%5Cu00eblle%22%2C%22lastName%22%3A%22Simon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vincent%22%2C%22lastName%22%3A%22G%5Cu00e9li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karine%22%2C%22lastName%22%3A%22Dubrana%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Palancade%22%7D%5D%2C%22abstractNote%22%3A%22Nuclear%20pore%20complexes%20%28NPCs%29%20have%20increasingly%20recognized%20interactions%20with%20the%20genome%2C%20as%20exemplified%20in%20yeast%2C%20where%20they%20bind%20transcribed%20or%20damaged%20chromatin.%20By%20combining%20genome-wide%20approaches%20with%20live%20imaging%20of%20model%20loci%2C%20we%20uncover%20a%20correlation%20between%20NPC%20association%20and%20the%20accumulation%20of%20R-loops%2C%20which%20are%20genotoxic%20structures%20formed%20through%20hybridization%20of%20nascent%20RNAs%20with%20their%20DNA%20templates.%20Manipulating%20hybrid%20formation%20demonstrates%20that%20R-loop%20accumulation%20per%20se%2C%20rather%20than%20transcription%20or%20R-loop-dependent%20damages%2C%20is%20the%20primary%20trigger%20for%20relocation%20to%20NPCs.%20Mechanistically%2C%20R-loop-dependent%20repositioning%20involves%20their%20recognition%20by%20the%20ssDNA-binding%20protein%20RPA%2C%20and%20SUMO-dependent%20interactions%20with%20NPC-associated%20factors.%20Preventing%20R-loop-dependent%20relocation%20leads%20to%20lethality%20in%20hybrid-accumulating%20conditions%2C%20while%20NPC%20tethering%20of%20a%20model%20hybrid-prone%20locus%20attenuates%20R-loop-dependent%20genetic%20instability.%20Remarkably%2C%20this%20relocation%20pathway%20involves%20molecular%20factors%20similar%20to%20those%20required%20for%20the%20association%20of%20stalled%20replication%20forks%20with%20NPCs%2C%20supporting%20the%20existence%20of%20convergent%20mechanisms%20for%20sensing%20transcriptional%20and%20genotoxic%20stresses.%22%2C%22date%22%3A%222023-09-20%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-023-41345-z%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-09-22T11%3A45%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22KGXBJR7R%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Challal%20et%20al.%22%2C%22parsedDate%22%3A%222023-06-06%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChallal%2C%20Drice%2C%20Alexandra%20Menant%2C%20Can%20Goksal%2C%20Estelle%20Leroy%2C%20Bassem%20Al-Sady%2C%20and%20Mathieu%20Rougemaille.%202023.%20%26%23x201C%3BA%20Dual%2C%20Catalytic%20Role%20for%20the%20Fission%20Yeast%20Ccr4-Not%20Complex%20in%20Gene%20Silencing%20and%20Heterochromatin%20Spreading.%26%23x201D%3B%20%3Ci%3EGenetics%3C%5C%2Fi%3E%2C%20June%2C%20iyad108.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgenetics%5C%2Fiyad108%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fgenetics%5C%2Fiyad108%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20dual%2C%20catalytic%20role%20for%20the%20fission%20yeast%20Ccr4-Not%20complex%20in%20gene%20silencing%20and%20heterochromatin%20spreading%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Drice%22%2C%22lastName%22%3A%22Challal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Menant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Can%22%2C%22lastName%22%3A%22Goksal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Estelle%22%2C%22lastName%22%3A%22Leroy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bassem%22%2C%22lastName%22%3A%22Al-Sady%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%5D%2C%22abstractNote%22%3A%22Heterochromatic%20gene%20silencing%20relies%20on%20combinatorial%20control%20by%20specific%20histone%20modifications%2C%20the%20occurrence%20of%20transcription%2C%20and%5C%2For%20RNA%20degradation.%20Once%20nucleated%2C%20heterochromatin%20propagates%20within%20defined%20chromosomal%20regions%20and%20is%20maintained%20throughout%20cell%20divisions%20to%20warrant%20proper%20genome%20expression%20and%20integrity.%20In%20the%20fission%20yeast%20Schizosaccharomyces%20pombe%2C%20the%20Ccr4-Not%20complex%20partakes%20in%20gene%20silencing%2C%20but%20its%20relative%20contribution%20to%20distinct%20heterochromatin%20domains%20and%20its%20role%20in%20nucleation%20versus%20spreading%20have%20remained%20elusive.%20Here%2C%20we%20unveil%20major%20functions%20for%20Ccr4-Not%20in%20silencing%20and%20heterochromatin%20spreading%20at%20the%20mating%20type%20locus%20and%20subtelomeres.%20Mutations%20of%20the%20catalytic%20subunits%20Caf1%20or%20Mot2%2C%20involved%20in%20RNA%20deadenylation%20and%20protein%20ubiquitinylation%20respectively%2C%20result%20in%20impaired%20propagation%20of%20H3K9me3%20and%20massive%20accumulation%20of%20nucleation-distal%20heterochromatic%20transcripts.%20Both%20silencing%20and%20spreading%20defects%20are%20suppressed%20upon%20disruption%20of%20the%20heterochromatin%20antagonizing%20factor%20Epe1.%20Overall%2C%20our%20results%20position%20the%20Ccr4-Not%20complex%20as%20a%20critical%2C%20dual%20regulator%20of%20heterochromatic%20gene%20silencing%20and%20spreading.%22%2C%22date%22%3A%222023-06-06%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fgenetics%5C%2Fiyad108%22%2C%22ISSN%22%3A%221943-2631%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-06-07T08%3A18%3A22Z%22%7D%7D%2C%7B%22key%22%3A%22J6CEZ7AD%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Matabishi-Bibi%20et%20al.%22%2C%22parsedDate%22%3A%222022-10-25%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMatabishi-Bibi%2C%20Laura%2C%20Drice%20Challal%2C%20Mara%20Barucco%2C%20Domenico%20Libri%2C%20and%20Anna%20Babour.%202022.%20%26%23x201C%3BTermination%20of%20the%20Unfolded%20Protein%20Response%20Is%20Guided%20by%20ER%20Stress-Induced%20HAC1%20MRNA%20Nuclear%20Retention.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2013%20%281%29%3A%206331.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-34133-8%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-022-34133-8%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Termination%20of%20the%20unfolded%20protein%20response%20is%20guided%20by%20ER%20stress-induced%20HAC1%20mRNA%20nuclear%20retention%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Matabishi-Bibi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Drice%22%2C%22lastName%22%3A%22Challal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mara%22%2C%22lastName%22%3A%22Barucco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Domenico%22%2C%22lastName%22%3A%22Libri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Babour%22%7D%5D%2C%22abstractNote%22%3A%22Cellular%20homeostasis%20is%20maintained%20by%20surveillance%20mechanisms%20that%20intervene%20at%20virtually%20every%20step%20of%20gene%20expression.%20In%20the%20nucleus%2C%20the%20yeast%20chromatin%20remodeler%20Isw1%20holds%20back%20maturing%20mRNA%20ribonucleoparticles%20to%20prevent%20their%20untimely%20export%2C%20but%20whether%20this%20activity%20operates%20beyond%20quality%20control%20of%20mRNA%20biogenesis%20to%20regulate%20gene%20expression%20is%20unknown.%20Here%2C%20we%20identify%20the%20mRNA%20encoding%20the%20central%20effector%20of%20the%20unfolded%20protein%20response%20%28UPR%29%20HAC1%2C%20as%20an%20Isw1%20RNA%20target.%20The%20direct%20binding%20of%20Isw1%20to%20the%203%5Cu2019%20untranslated%20region%20of%20HAC1%20mRNA%20restricts%20its%20nuclear%20export%20and%20is%20required%20for%20accurate%20UPR%20abatement.%20Accordingly%2C%20ISW1%20inactivation%20sensitizes%20cells%20to%20endoplasmic%20reticulum%20%28ER%29%20stress%20while%20its%20overexpression%20reduces%20UPR%20induction.%20Our%20results%20reveal%20an%20unsuspected%20mechanism%2C%20in%20which%20binding%20of%20ER-stress%20induced%20Isw1%20to%20HAC1%20mRNA%20limits%20its%20nuclear%20export%2C%20providing%20a%20feedback%20loop%20that%20fine-tunes%20UPR%20attenuation%20to%20guarantee%20homeostatic%20adaptation%20to%20ER%20stress.%22%2C%22date%22%3A%222022-10-25%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-022-34133-8%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-022-34133-8%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-01-25T12%3A02%3A53Z%22%7D%7D%2C%7B%22key%22%3A%22EQSFVWQX%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mart%5Cu00edn%20Caballero%20et%20al.%22%2C%22parsedDate%22%3A%222022-09-19%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMart%26%23xED%3Bn%20Caballero%2C%20Luc%26%23xED%3Ba%2C%20Mat%26%23xED%3Bas%20Capella%2C%20Ram%26%23xF3%3Bn%20Ramos%20Barrales%2C%20Nikolay%20Dobrev%2C%20Thomas%20van%20Emden%2C%20Yasuhiro%20Hirano%2C%20Vishnu%20N.%20Suma%20Sreechakram%2C%20et%20al.%202022.%20%26%23x201C%3BThe%20Inner%20Nuclear%20Membrane%20Protein%20Lem2%20Coordinates%20RNA%20Degradation%20at%20the%20Nuclear%20Periphery.%26%23x201D%3B%20%3Ci%3ENature%20Structural%20%26amp%3B%20Molecular%20Biology%3C%5C%2Fi%3E%2029%20%289%29%3A%20910%26%23x2013%3B21.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-022-00831-6%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-022-00831-6%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20inner%20nuclear%20membrane%20protein%20Lem2%20coordinates%20RNA%20degradation%20at%20the%20nuclear%20periphery%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luc%5Cu00eda%22%2C%22lastName%22%3A%22Mart%5Cu00edn%20Caballero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mat%5Cu00edas%22%2C%22lastName%22%3A%22Capella%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ram%5Cu00f3n%20Ramos%22%2C%22lastName%22%3A%22Barrales%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nikolay%22%2C%22lastName%22%3A%22Dobrev%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22van%20Emden%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yasuhiro%22%2C%22lastName%22%3A%22Hirano%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vishnu%20N.%22%2C%22lastName%22%3A%22Suma%20Sreechakram%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Fischer-Burkart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yasuha%22%2C%22lastName%22%3A%22Kinugasa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alicia%22%2C%22lastName%22%3A%22Nevers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Irmgard%22%2C%22lastName%22%3A%22Sinning%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tam%5Cu00e1s%22%2C%22lastName%22%3A%22Fischer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yasushi%22%2C%22lastName%22%3A%22Hiraoka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurd%22%2C%22lastName%22%3A%22Braun%22%7D%5D%2C%22abstractNote%22%3A%22Transcriptionally%20silent%20chromatin%20often%20localizes%20to%20the%20nuclear%20periphery.%20However%2C%20whether%20the%20nuclear%20envelope%20%28NE%29%20is%20a%20site%20for%20post-transcriptional%20gene%20repression%20is%20not%20well%20understood.%20Here%20we%20demonstrate%20that%20Schizosaccharomyces%20pombe%20Lem2%2C%20an%20NE%20protein%2C%20regulates%20nuclear-exosome-mediated%20RNA%20degradation.%20Lem2%20deletion%20causes%20accumulation%20of%20RNA%20precursors%20and%20meiotic%20transcripts%20and%20de-localization%20of%20an%20engineered%20exosome%20substrate%20from%20the%20nuclear%20periphery.%20Lem2%20does%20not%20directly%20bind%20RNA%20but%20instead%20interacts%20with%20the%20exosome-targeting%20MTREC%20complex%20and%20its%20human%20homolog%20PAXT%20to%20promote%20RNA%20recruitment.%20This%20pathway%20acts%20largely%20independently%20of%20nuclear%20bodies%20where%20exosome%20factors%20assemble.%20Nutrient%20availability%20modulates%20Lem2%20regulation%20of%20meiotic%20transcripts%2C%20implying%20that%20this%20pathway%20is%20environmentally%20responsive.%20Our%20work%20reveals%20that%20multiple%20spatially%20distinct%20degradation%20pathways%20exist.%20Among%20these%2C%20Lem2%20coordinates%20RNA%20surveillance%20of%20meiotic%20transcripts%20and%20non-coding%20RNAs%20by%20recruiting%20exosome%20co-factors%20to%20the%20nuclear%20periphery.%22%2C%22date%22%3A%222022-09-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41594-022-00831-6%22%2C%22ISSN%22%3A%221545-9985%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41594-022-00831-6%22%2C%22collections%22%3A%5B%22GCG5UQZ2%22%5D%2C%22dateModified%22%3A%222023-12-14T15%3A29%3A48Z%22%7D%7D%2C%7B%22key%22%3A%22R49JF7ZT%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Finet%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-15%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFinet%2C%20Olivier%2C%20Carlo%20Yague-Sanz%2C%20Lara%20Katharina%20Kr%26%23xFC%3Bger%2C%20Phong%20Tran%2C%20Val%26%23xE9%3Brie%20Migeot%2C%20Max%20Louski%2C%20Alicia%20Nevers%2C%20et%20al.%202021.%20%26%23x201C%3BTranscription-Wide%20Mapping%20of%20Dihydrouridine%20Reveals%20That%20MRNA%20Dihydrouridylation%20Is%20Required%20for%20Meiotic%20Chromosome%20Segregation.%26%23x201D%3B%20%3Ci%3EMolecular%20Cell%3C%5C%2Fi%3E%2C%20November%2C%20S1097-2765%2821%2900953-9.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.molcel.2021.11.003%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.molcel.2021.11.003%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Transcription-wide%20mapping%20of%20dihydrouridine%20reveals%20that%20mRNA%20dihydrouridylation%20is%20required%20for%20meiotic%20chromosome%20segregation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Finet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carlo%22%2C%22lastName%22%3A%22Yague-Sanz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lara%20Katharina%22%2C%22lastName%22%3A%22Kr%5Cu00fcger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Phong%22%2C%22lastName%22%3A%22Tran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Val%5Cu00e9rie%22%2C%22lastName%22%3A%22Migeot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Max%22%2C%22lastName%22%3A%22Louski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alicia%22%2C%22lastName%22%3A%22Nevers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jingjing%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Felix%20G.%20M.%22%2C%22lastName%22%3A%22Ernst%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ludivine%22%2C%22lastName%22%3A%22Wacheul%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maxime%22%2C%22lastName%22%3A%22Wery%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonin%22%2C%22lastName%22%3A%22Morillon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Dedon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denis%20L.%20J.%22%2C%22lastName%22%3A%22Lafontaine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Damien%22%2C%22lastName%22%3A%22Hermand%22%7D%5D%2C%22abstractNote%22%3A%22The%20epitranscriptome%20has%20emerged%20as%20a%20new%20fundamental%20layer%20of%20control%20of%20gene%20expression.%20Nevertheless%2C%20the%20determination%20of%20the%20transcriptome-wide%20occupancy%20and%20function%20of%20RNA%20modifications%20remains%20challenging.%20Here%20we%20have%20developed%20Rho-seq%2C%20an%20integrated%20pipeline%20detecting%20a%20range%20of%20modifications%20through%20differential%20modification-dependent%20rhodamine%20labeling.%20Using%20Rho-seq%2C%20we%20confirm%20that%20the%20reduction%20of%20uridine%20to%20dihydrouridine%20%28D%29%20by%20the%20Dus%20reductase%20enzymes%20targets%20tRNAs%20in%20E.%5Cu00a0coli%20and%20fission%20yeast.%20We%20find%20that%20the%20D%20modification%20is%20also%20present%20on%20fission%20yeast%20mRNAs%2C%20particularly%20those%20encoding%20cytoskeleton-related%20proteins%2C%20which%20is%20supported%20by%20large-scale%20proteome%20analyses%20and%20ribosome%20profiling.%20We%20show%20that%20the%20%5Cu03b1-tubulin%20encoding%20mRNA%20nda2%20undergoes%20Dus3-dependent%20dihydrouridylation%2C%20which%20affects%20its%20translation.%20The%20absence%20of%20the%20modification%20on%20nda2%20mRNA%20strongly%20impacts%20meiotic%20chromosome%20segregation%2C%20resulting%20in%20low%20gamete%20viability.%20Applying%20Rho-seq%20to%20human%20cells%20revealed%20that%20tubulin%20mRNA%20dihydrouridylation%20is%20evolutionarily%20conserved.%22%2C%22date%22%3A%222021-11-15%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.molcel.2021.11.003%22%2C%22ISSN%22%3A%221097-4164%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222021-12-02T14%3A58%3A29Z%22%7D%7D%2C%7B%22key%22%3A%22IQBWNI38%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Andric%20and%20Rougemaille%22%2C%22parsedDate%22%3A%222021-06-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAndric%2C%20Vedrana%2C%20and%20Mathieu%20Rougemaille.%202021.%20%26%23x201C%3BLong%20Non-Coding%20RNAs%20in%20the%20Control%20of%20Gametogenesis%3A%20Lessons%20from%20Fission%20Yeast.%26%23x201D%3B%20%3Ci%3ENon-Coding%20RNA%3C%5C%2Fi%3E%207%20%282%29%3A%2034.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fncrna7020034%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fncrna7020034%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Long%20Non-Coding%20RNAs%20in%20the%20Control%20of%20Gametogenesis%3A%20Lessons%20from%20Fission%20Yeast%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vedrana%22%2C%22lastName%22%3A%22Andric%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%5D%2C%22abstractNote%22%3A%22Long%20non-coding%20RNAs%20%28lncRNAs%29%20contribute%20to%20cell%20fate%20decisions%20by%20modulating%20genome%20expression%20and%20stability.%20In%20the%20fission%20yeast%20Schizosaccharomyces%20pombe%2C%20the%20transition%20from%20mitosis%20to%20meiosis%20results%20in%20a%20marked%20remodeling%20of%20gene%20expression%20profiles%2C%20which%20ultimately%20ensures%20gamete%20production%20and%20inheritance%20of%20genetic%20information%20to%20the%20offspring.%20This%20key%20developmental%20process%20involves%20a%20set%20of%20dedicated%20lncRNAs%20that%20shape%20cell%20cycle-dependent%20transcriptomes%20through%20a%20variety%20of%20mechanisms%2C%20including%20epigenetic%20modifications%20and%20the%20modulation%20of%20transcription%2C%20post-transcriptional%20and%20post-translational%20regulations%2C%20and%20that%20contribute%20to%20meiosis-specific%20chromosomal%20events.%20In%20this%20review%2C%20we%20summarize%20the%20biology%20of%20these%20lncRNAs%2C%20from%20their%20identification%20to%20mechanism%20of%20action%2C%20and%20discuss%20their%20regulatory%20role%20in%20the%20control%20of%20gametogenesis.%22%2C%22date%22%3A%222021-06-11%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fncrna7020034%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2311-553X%5C%2F7%5C%2F2%5C%2F34%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222022-06-07T11%3A54%3A52Z%22%7D%7D%2C%7B%22key%22%3A%22SFJ7BYZ7%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Andric%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-03%22%2C%22numChildren%22%3A4%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAndric%2C%20Vedrana%2C%20Alicia%20Nevers%2C%20Ditipriya%20Hazra%2C%20Sylvie%20Auxilien%2C%20Alexandra%20Menant%2C%20Marc%20Graille%2C%20Benoit%20Palancade%2C%20and%20Mathieu%20Rougemaille.%202021.%20%26%23x201C%3BA%20Scaffold%20LncRNA%20Shapes%20the%20Mitosis%20to%20Meiosis%20Switch.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2012%20%281%29%3A%20770.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-021-21032-7%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-021-21032-7%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20scaffold%20lncRNA%20shapes%20the%20mitosis%20to%20meiosis%20switch%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vedrana%22%2C%22lastName%22%3A%22Andric%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alicia%22%2C%22lastName%22%3A%22Nevers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ditipriya%22%2C%22lastName%22%3A%22Hazra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Auxilien%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Menant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc%22%2C%22lastName%22%3A%22Graille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Palancade%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%5D%2C%22abstractNote%22%3A%22Long%20non-coding%20RNAs%20%28lncRNAs%29%20contribute%20to%20the%20regulation%20of%20gene%20expression%20in%20response%20to%20intra-%20or%20extracellular%20signals%20but%20the%20underlying%20molecular%20mechanisms%20remain%20largely%20unexplored.%20Here%2C%20we%20identify%20an%20uncharacterized%20lncRNA%20as%20a%20central%20player%20in%20shaping%20the%20meiotic%20gene%20expression%20program%20in%20fission%20yeast.%20We%20report%20that%20this%20regulatory%20RNA%2C%20termed%20mamRNA%2C%20scaffolds%20the%20antagonistic%20RNA-binding%20proteins%20Mmi1%20and%20Mei2%20to%20ensure%20their%20reciprocal%20inhibition%20and%20fine%20tune%20meiotic%20mRNA%20degradation%20during%20mitotic%20growth.%20Mechanistically%2C%20mamRNA%20allows%20Mmi1%20to%20target%20Mei2%20for%20ubiquitin-mediated%20downregulation%2C%20and%20conversely%20enables%20accumulating%20Mei2%20to%20impede%20Mmi1%20activity%2C%20thereby%20reinforcing%20the%20mitosis%20to%20meiosis%20switch.%20These%20regulations%20also%20occur%20within%20a%20unique%20Mmi1-containing%20nuclear%20body%2C%20positioning%20mamRNA%20as%20a%20spatially-confined%20sensor%20of%20Mei2%20levels.%20Our%20results%20thus%20provide%20a%20mechanistic%20basis%20for%20the%20mutual%20control%20of%20gametogenesis%20effectors%20and%20further%20expand%20our%20vision%20of%20the%20regulatory%20potential%20of%20lncRNAs.%22%2C%22date%22%3A%222021-02-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-021-21032-7%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-021-21032-7%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222021-03-16T10%3A13%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22QS5AJ49N%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Graille%20and%20Rougemaille%22%2C%22parsedDate%22%3A%222020-08%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGraille%2C%20Marc%2C%20and%20Mathieu%20Rougemaille.%202020.%20%26%23x201C%3BERH%20Proteins%3A%20Connecting%20RNA%20Processing%20to%20Tumorigenesis%3F%26%23x201D%3B%20%3Ci%3ECurrent%20Genetics%3C%5C%2Fi%3E%2066%20%284%29%3A%20689%26%23x2013%3B92.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00294-020-01065-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00294-020-01065-z%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22ERH%20proteins%3A%20connecting%20RNA%20processing%20to%20tumorigenesis%3F%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc%22%2C%22lastName%22%3A%22Graille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%5D%2C%22abstractNote%22%3A%22With%20the%20development%20of%20-omics%20approaches%2C%20the%20scientific%20community%20is%20now%20submerged%20by%20a%20wealth%20of%20information%20that%20can%20be%20used%20to%20analyze%20various%20parameters%3A%20the%20degree%20of%20protein%20sequence%20conservation%2C%20protein%203D%20structures%20as%20well%20as%20RNA%20and%20protein%20expression%20levels%20in%20various%20benign%20and%20tumor%20tissues%2C%20during%20organism%20development%20or%20upon%20exposure%20to%20chemicals%20such%20as%20endocrine%20disrupters.%20However%2C%20if%20such%20information%20can%20be%20used%20to%20identify%20genes%20with%20potentially%20important%20biological%20function%2C%20additional%20studies%20are%20needed%20to%20deeply%20characterize%20their%20cellular%20function%20in%20model%20organisms.%20Here%2C%20we%20discuss%20the%20case%20of%20such%20a%20gene%3A%20ERH%2C%20encoding%20a%20highly%20conserved%20homodimeric%20protein%20found%20in%20unicellular%20eukaryotes%2C%20plants%20and%20metazoan%2C%20of%20yet%20unknown%20biological%20function%2C%20which%20might%20be%20linked%20to%20mRNA%20metabolism%20and%20that%20is%20emerging%20as%20important%20for%20cell%20migration%20and%20metastasis.%22%2C%22date%22%3A%22AUG%202020%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00294-020-01065-z%22%2C%22ISSN%22%3A%221432-0983%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222020-09-01T12%3A57%3A38Z%22%7D%7D%2C%7B%22key%22%3A%223B7RMWNS%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hazra%20et%20al.%22%2C%22parsedDate%22%3A%222020-01-23%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHazra%2C%20Ditipriya%2C%20Vedrana%20Andri%26%23x107%3B%2C%20Benoit%20Palancade%2C%20Mathieu%20Rougemaille%2C%20and%20Marc%20Graille.%202020.%20%26%23x201C%3BFormation%20of%20S.%20Pombe%20Erh1%20Homodimer%20Mediates%20Gametogenic%20Gene%20Silencing%20and%20Meiosis%20Progression.%26%23x201D%3B%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2010%20%281%29%3A%201034.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-57872-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-57872-4%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Formation%20of%20S.%20pombe%20Erh1%20homodimer%20mediates%20gametogenic%20gene%20silencing%20and%20meiosis%20progression%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ditipriya%22%2C%22lastName%22%3A%22Hazra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vedrana%22%2C%22lastName%22%3A%22Andri%5Cu0107%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Palancade%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marc%22%2C%22lastName%22%3A%22Graille%22%7D%5D%2C%22abstractNote%22%3A%22Timely%20and%20accurate%20expression%20of%20the%20genetic%20information%20relies%20on%20the%20integration%20of%20environmental%20cues%20and%20the%20activation%20of%20regulatory%20networks%20involving%20transcriptional%20and%20post-transcriptional%20mechanisms.%20In%20fission%20yeast%2C%20meiosis-specific%20transcripts%20are%20selectively%20targeted%20for%20degradation%20during%20mitosis%20by%20the%20EMC%20complex%2C%20composed%20of%20Erh1%2C%20the%20ortholog%20of%20human%20ERH%2C%20and%20the%20YTH%20family%20RNA-binding%20protein%20Mmi1.%20Here%2C%20we%20present%20the%20crystal%20structure%20of%20Erh1%20and%20show%20that%20it%20assembles%20as%20a%20homodimer.%20Mutations%20of%20amino%20acid%20residues%20to%20disrupt%20Erh1%20homodimer%20formation%20result%20in%20loss-of-function%20phenotypes%2C%20similar%20to%20erh1%5Cu2206%20cells%3A%20expression%20of%20meiotic%20genes%20is%20derepressed%20in%20mitotic%20cells%20and%20meiosis%20progression%20is%20severely%20compromised.%20Interestingly%2C%20formation%20of%20Erh1%20homodimer%20is%20dispensable%20for%20interaction%20with%20Mmi1%2C%20suggesting%20that%20only%20fully%20assembled%20EMC%20complexes%20consisting%20of%20two%20Mmi1%20molecules%20bridged%20by%20an%20Erh1%20dimer%20are%20functionally%20competent.%20We%20also%20show%20that%20Erh1%20does%20not%20contribute%20to%20Mmi1-dependent%20down-regulation%20of%20the%20meiosis%20regulator%20Mei2%2C%20supporting%20the%20notion%20that%20Mmi1%20performs%20additional%20functions%20beyond%20EMC.%20Overall%2C%20our%20results%20provide%20a%20structural%20basis%20for%20the%20assembly%20of%20the%20EMC%20complex%20and%20highlight%20its%20biological%20relevance%20in%20gametogenic%20gene%20silencing%20and%20meiosis%20progression.%22%2C%22date%22%3A%22Jan%2023%2C%202020%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-020-57872-4%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%2C%222FBUFWW8%22%5D%2C%22dateModified%22%3A%222020-01-27T08%3A39%3A41Z%22%7D%7D%2C%7B%22key%22%3A%22584XALEK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Simonetti%20et%20al.%22%2C%22parsedDate%22%3A%222017-08-25%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESimonetti%2C%20Fabrizio%2C%20Tito%20Candelli%2C%20Sebastien%20Leon%2C%20Domenico%20Libri%2C%20and%20Mathieu%20Rougemaille.%202017.%20%26%23x201C%3BUbiquitination-Dependent%20Control%20of%20Sexual%20Differentiation%20in%20Fission%20Yeast.%26%23x201D%3B%20Edited%20by%20Torben%20Heick%20Jensen.%20%3Ci%3EELife%3C%5C%2Fi%3E%206%20%28August%29%3Ae28046.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.28046%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.28046%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ubiquitination-dependent%20control%20of%20sexual%20differentiation%20in%20fission%20yeast%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabrizio%22%2C%22lastName%22%3A%22Simonetti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tito%22%2C%22lastName%22%3A%22Candelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sebastien%22%2C%22lastName%22%3A%22Leon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Domenico%22%2C%22lastName%22%3A%22Libri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Rougemaille%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Torben%20Heick%22%2C%22lastName%22%3A%22Jensen%22%7D%5D%2C%22abstractNote%22%3A%22In%20fission%20yeast%2C%20meiosis-specific%20transcripts%20are%20selectively%20eliminated%20during%20vegetative%20growth%20by%20the%20combined%20action%20of%20the%20YTH-family%20RNA-binding%20protein%20Mmi1%20and%20the%20nuclear%20exosome.%20Upon%20nutritional%20starvation%2C%20the%20master%20regulator%20of%20meiosis%20Mei2%20inactivates%20Mmi1%2C%20thereby%20allowing%20expression%20of%20the%20meiotic%20program.%20Here%2C%20we%20show%20that%20the%20E3%20ubiquitin%20ligase%20subunit%20Not4%5C%2FMot2%20of%20the%20evolutionarily%20conserved%20Ccr4-Not%20complex%2C%20which%20associates%20with%20Mmi1%2C%20promotes%20suppression%20of%20meiotic%20transcripts%20expression%20in%20mitotic%20cells.%20Our%20analyses%20suggest%20that%20Mot2%20directs%20ubiquitination%20of%20Mei2%20to%20preserve%20the%20activity%20of%20Mmi1%20during%20vegetative%20growth.%20Importantly%2C%20Mot2%20is%20not%20involved%20in%20the%20constitutive%20pathway%20of%20Mei2%20turnover%2C%20but%20rather%20plays%20a%20regulatory%20role%20to%20limit%20its%20accumulation%20or%20inhibit%20its%20function.%20We%20propose%20that%20Mmi1%20recruits%20the%20Ccr4-Not%20complex%20to%20counteract%20its%20own%20inhibitor%20Mei2%2C%20thereby%20locking%20the%20system%20in%20a%20stable%20state%20that%20ensures%20the%20repression%20of%20the%20meiotic%20program%20by%20Mmi1.%22%2C%22date%22%3A%22August%2025%2C%202017%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.7554%5C%2FeLife.28046%22%2C%22ISSN%22%3A%222050-084X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.28046%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-03-11T14%3A47%3A48Z%22%7D%7D%5D%7D
Penzo, Arianna, Marion Dubarry, Clémentine Brocas, Myriam Zheng, Raphaël M. Mangione, Mathieu Rougemaille, Coralie Goncalves, et al. 2023. “A R-Loop Sensing Pathway Mediates the Relocation of Transcribed Genes to Nuclear Pore Complexes.” Nature Communications 14 (1): 5606. https://doi.org/10.1038/s41467-023-41345-z.
Challal, Drice, Alexandra Menant, Can Goksal, Estelle Leroy, Bassem Al-Sady, and Mathieu Rougemaille. 2023. “A Dual, Catalytic Role for the Fission Yeast Ccr4-Not Complex in Gene Silencing and Heterochromatin Spreading.” Genetics, June, iyad108. https://doi.org/10.1093/genetics/iyad108.
Matabishi-Bibi, Laura, Drice Challal, Mara Barucco, Domenico Libri, and Anna Babour. 2022. “Termination of the Unfolded Protein Response Is Guided by ER Stress-Induced HAC1 MRNA Nuclear Retention.” Nature Communications 13 (1): 6331. https://doi.org/10.1038/s41467-022-34133-8.
Martín Caballero, Lucía, Matías Capella, Ramón Ramos Barrales, Nikolay Dobrev, Thomas van Emden, Yasuhiro Hirano, Vishnu N. Suma Sreechakram, et al. 2022. “The Inner Nuclear Membrane Protein Lem2 Coordinates RNA Degradation at the Nuclear Periphery.” Nature Structural & Molecular Biology 29 (9): 910–21. https://doi.org/10.1038/s41594-022-00831-6.
Finet, Olivier, Carlo Yague-Sanz, Lara Katharina Krüger, Phong Tran, Valérie Migeot, Max Louski, Alicia Nevers, et al. 2021. “Transcription-Wide Mapping of Dihydrouridine Reveals That MRNA Dihydrouridylation Is Required for Meiotic Chromosome Segregation.” Molecular Cell, November, S1097-2765(21)00953-9. https://doi.org/10.1016/j.molcel.2021.11.003.
Andric, Vedrana, and Mathieu Rougemaille. 2021. “Long Non-Coding RNAs in the Control of Gametogenesis: Lessons from Fission Yeast.” Non-Coding RNA 7 (2): 34. https://doi.org/10.3390/ncrna7020034.
Andric, Vedrana, Alicia Nevers, Ditipriya Hazra, Sylvie Auxilien, Alexandra Menant, Marc Graille, Benoit Palancade, and Mathieu Rougemaille. 2021. “A Scaffold LncRNA Shapes the Mitosis to Meiosis Switch.” Nature Communications 12 (1): 770. https://doi.org/10.1038/s41467-021-21032-7.
Graille, Marc, and Mathieu Rougemaille. 2020. “ERH Proteins: Connecting RNA Processing to Tumorigenesis?” Current Genetics 66 (4): 689–92. https://doi.org/10.1007/s00294-020-01065-z.
Hazra, Ditipriya, Vedrana Andrić, Benoit Palancade, Mathieu Rougemaille, and Marc Graille. 2020. “Formation of S. Pombe Erh1 Homodimer Mediates Gametogenic Gene Silencing and Meiosis Progression.” Scientific Reports 10 (1): 1034. https://doi.org/10.1038/s41598-020-57872-4.
Simonetti, Fabrizio, Tito Candelli, Sebastien Leon, Domenico Libri, and Mathieu Rougemaille. 2017. “Ubiquitination-Dependent Control of Sexual Differentiation in Fission Yeast.” Edited by Torben Heick Jensen. ELife 6 (August):e28046. https://doi.org/10.7554/eLife.28046.