Biological High-Field Magnetic Resonance
HFEPR is a powerful biophysical tool for investigating the magnetic centers within biological systems.
The position and bonding of radicals and metal ions as well as the structure of the bio-macromolecules can be established using an array of HFEPR techniques.
We specialize in the application of high magnetic-field and high frequency electron paramagnetic resonance (HFEPR) techniques for solving physical, chemical and biological problems. This involves a wide range of activities that include design and construction of instruments, computational and synthetic chemistry, as well as biochemistry and molecular biology. We have been involved in projects ranging from environmental chemistry to large proteins to molecular magnets with collaborators worldwide.
Our major biochemical focus has been on the characterization of radicals and Mn(II) centers in proteins. Both of these areas are strongly tied to research in oxidative stress and photosynthesis. The list of Mn(II) proteins that we are studying has been steadily growing and include those involved in the regulation and transport of manganese in cells as well as many other important biological functions ranging from regulation of oxidative stress and bacterial virulence to breakdown of organic molecules. We have extended our methodology to characterizing these paramagnetic centers inside intact viable cells.
We have extensive experience in the synthesis of Mn(II) complexes that help us understand the EPR spectroscopy of Mn(II) containing proteins. Some of these complexes are also functional mimics and provide valuable frameworks for examining the mechanisms of Mn(II) proteins and the design principles required for metal based drugs that mimic these enzymes. To complement HFEPR measurements, we use quantum chemical methods to calculate structures and magnetic spin parameters from first principles and molecular biology to not only test the spectroscopic measurements and quantum calculations, but also to probe aspects of enzyme function.
Recently, we have been interested in using HFEPR PELDOR (Pulse Electron Double Resonance) to determine the structure and structural changes of bio-macromolecules. By measuring the magnetic dipole coupling between unpaired electrons, precise nanometer scale distances can be obtained. Paramagnetic centers can be endogenous to the biological system or introduced as spin labels, and we have begun developing new spin labels and spectroscopic protocols for various applications.
For all the publications of the Team click on the button below.