Replication and assembly of poxviruses
Publications
3888256
RAPUS
chicago-author-date
50
date
desc
year
31667
https://www.i2bc.paris-saclay.fr/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-708aa7d93246ddb6bd4223838ce37799%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22554UCT73%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hofstadter%20et%20al.%22%2C%22parsedDate%22%3A%222024-08-27%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHofstadter%2C%20William%20A.%2C%20Katelyn%20C.%20Cook%2C%20Elene%20Tsopurashvili%2C%20Robert%20Gebauer%2C%20Vojt%26%23x11B%3Bch%20Pra%26%23x17E%3B%26%23xE1%3Bk%2C%20Emily%20A.%20Machala%2C%20Ji%20Woo%20Park%2C%20Kay%20Gr%26%23xFC%3Bnewald%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20and%20Ileana%20M.%20Cristea.%202024.%20%26%23x201C%3BInfection-Induced%20Peripheral%20Mitochondria%20Fission%20Drives%20ER%20Encapsulations%20and%20Inter-Mitochondria%20Contacts%20That%20Rescue%20Bioenergetics.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2015%20%281%29%3A%207352.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-024-51680-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-024-51680-4%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Infection-induced%20peripheral%20mitochondria%20fission%20drives%20ER%20encapsulations%20and%20inter-mitochondria%20contacts%20that%20rescue%20bioenergetics%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%20A.%22%2C%22lastName%22%3A%22Hofstadter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katelyn%20C.%22%2C%22lastName%22%3A%22Cook%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elene%22%2C%22lastName%22%3A%22Tsopurashvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Gebauer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22Pra%5Cu017e%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%20A.%22%2C%22lastName%22%3A%22Machala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ji%20Woo%22%2C%22lastName%22%3A%22Park%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ileana%20M.%22%2C%22lastName%22%3A%22Cristea%22%7D%5D%2C%22abstractNote%22%3A%22The%20dynamic%20regulation%20of%20mitochondria%20shape%20via%20fission%20and%20fusion%20is%20critical%20for%20cellular%20responses%20to%20stimuli.%20In%20homeostatic%20cells%2C%20two%20modes%20of%20mitochondrial%20fission%2C%20midzone%20and%20peripheral%2C%20provide%20a%20decision%20fork%20between%20either%20proliferation%20or%20clearance%20of%20mitochondria.%20However%2C%20the%20relationship%20between%20specific%20mitochondria%20shapes%20and%20functions%20remains%20unclear%20in%20many%20biological%20contexts.%20While%20commonly%20associated%20with%20decreased%20bioenergetics%2C%20fragmented%20mitochondria%20paradoxically%20exhibit%20elevated%20respiration%20in%20several%20disease%20states%2C%20including%20infection%20with%20the%20prevalent%20pathogen%20human%20cytomegalovirus%20%28HCMV%29%20and%20metastatic%20melanoma.%20Here%2C%20incorporating%20super-resolution%20microscopy%20with%20mass%20spectrometry%20and%20metabolic%20assays%2C%20we%20use%20HCMV%20infection%20to%20establish%20a%20molecular%20mechanism%20for%20maintaining%20respiration%20within%20a%20fragmented%20mitochondria%20population.%20We%20establish%20that%20HCMV%20induces%20fragmentation%20through%20peripheral%20mitochondrial%20fission%20coupled%20with%20suppression%20of%20mitochondria%20fusion.%20Unlike%20uninfected%20cells%2C%20the%20progeny%20of%20peripheral%20fission%20enter%20mitochondria-ER%20encapsulations%20%28MENCs%29%20where%20they%20are%20protected%20from%20degradation%20and%20bioenergetically%20stabilized%20during%20infection.%20MENCs%20also%20stabilize%20pro-viral%20inter-mitochondria%20contacts%20%28IMCs%29%2C%20which%20electrochemically%20link%20mitochondria%20and%20promote%20respiration.%20Demonstrating%20a%20broader%20relevance%2C%20we%20show%20that%20the%20fragmented%20mitochondria%20within%20metastatic%20melanoma%20cells%20also%20form%20MENCs.%20Our%20findings%20establish%20a%20mechanism%20where%20mitochondria%20fragmentation%20can%20promote%20increased%20respiration%2C%20a%20feature%20relevant%20in%20the%20context%20of%20human%20diseases.%22%2C%22date%22%3A%222024-08-27%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-024-51680-4%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-10-04T06%3A15%3A30Z%22%7D%7D%2C%7B%22key%22%3A%22ZCY9ZEBG%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Williams%20et%20al.%22%2C%22parsedDate%22%3A%222024-05-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWilliams%2C%20Harry%20M.%2C%20Sigurdur%20R.%20Thorkelsson%2C%20Dominik%20Vogel%2C%20Carola%20Busch%2C%20Morlin%20Milewski%2C%20Stephen%20Cusack%2C%20Kay%20Gr%26%23xFC%3Bnewald%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20and%20Maria%20Rosenthal.%202024.%20%26%23x201C%3BStructural%20Snapshots%20of%20Phenuivirus%20Cap-Snatching%20and%20Transcription.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2C%20May%2C%20gkae330.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae330%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkae330%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20snapshots%20of%20phenuivirus%20cap-snatching%20and%20transcription%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harry%20M.%22%2C%22lastName%22%3A%22Williams%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20R.%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominik%22%2C%22lastName%22%3A%22Vogel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carola%22%2C%22lastName%22%3A%22Busch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Morlin%22%2C%22lastName%22%3A%22Milewski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%22%2C%22lastName%22%3A%22Cusack%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Rosenthal%22%7D%5D%2C%22abstractNote%22%3A%22Severe%20fever%20with%20thrombocytopenia%20syndrome%20virus%20%28SFTSV%29%20is%20a%20human%20pathogen%20that%20is%20now%20endemic%20to%20several%20East%20Asian%20countries.%20The%20viral%20large%20%28L%29%20protein%20catalyzes%20viral%20transcription%20by%20stealing%20host%20mRNA%20caps%20via%20a%20process%20known%20as%20cap-snatching.%20Here%2C%20we%20establish%20an%20in%20vitro%20cap-snatching%20assay%20and%20present%20three%20high-quality%20electron%20cryo-microscopy%20%28cryo-EM%29%20structures%20of%20the%20SFTSV%20L%20protein%20in%20biologically%20relevant%2C%20transcription-specific%20states.%20In%20a%20priming-state%20structure%2C%20we%20show%20capped%20RNA%20bound%20to%20the%20L%20protein%20cap-binding%20domain%20%28CBD%29.%20The%20L%20protein%20conformation%20in%20this%20priming%20structure%20is%20significantly%20different%20from%20published%20replication-state%20structures%2C%20in%20particular%20the%20N-%20and%20C-terminal%20domains.%20The%20capped-RNA%20is%20positioned%20in%20a%20way%20that%20it%20can%20feed%20directly%20into%20the%20RNA-dependent%20RNA%20polymerase%20%28RdRp%29%20ready%20for%20elongation.%20We%20also%20captured%20the%20L%20protein%20in%20an%20early-elongation%20state%20following%20primer-incorporation%20demonstrating%20that%20this%20priming%20conformation%20is%20retained%20at%20least%20in%20the%20very%20early%20stages%20of%20primer%20extension.%20This%20structural%20data%20is%20complemented%20by%20in%20vitro%20biochemical%20and%20cell-based%20assays.%20Together%2C%20these%20insights%20further%20our%20mechanistic%20understanding%20of%20how%20SFTSV%20and%20other%20bunyaviruses%20incorporate%20stolen%20host%20mRNA%20fragments%20into%20their%20viral%20transcripts%20thereby%20allowing%20the%20virus%20to%20hijack%20host%20cell%20translation%20machinery.%22%2C%22date%22%3A%222024-05-06%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkae330%22%2C%22ISSN%22%3A%221362-4962%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-05-22T09%3A32%3A40Z%22%7D%7D%2C%7B%22key%22%3A%22RUPJIG2W%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Liu%20et%20al.%22%2C%22parsedDate%22%3A%222024-02-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELiu%2C%20Jiasui%2C%20Simon%20Corroyer-Dulmont%2C%20Vojt%26%23x11B%3Bch%20Pra%26%23x17E%3B%26%23xE1%3Bk%2C%20Iskander%20Khusainov%2C%20Karola%20Bahrami%2C%20Sonja%20Welsch%2C%20Daven%20Vasishtan%2C%20et%20al.%202024.%20%26%23x201C%3BThe%20Palisade%20Layer%20of%20the%20Poxvirus%20Core%20Is%20Composed%20of%20Flexible%20A10%20Trimers.%26%23x201D%3B%20%3Ci%3ENature%20Structural%20%26amp%3B%20Molecular%20Biology%3C%5C%2Fi%3E%2C%20February.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-024-01218-5%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41594-024-01218-5%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20palisade%20layer%20of%20the%20poxvirus%20core%20is%20composed%20of%20flexible%20A10%20trimers%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiasui%22%2C%22lastName%22%3A%22Liu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simon%22%2C%22lastName%22%3A%22Corroyer-Dulmont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22Pra%5Cu017e%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Iskander%22%2C%22lastName%22%3A%22Khusainov%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karola%22%2C%22lastName%22%3A%22Bahrami%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonja%22%2C%22lastName%22%3A%22Welsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daven%22%2C%22lastName%22%3A%22Vasishtan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agnieszka%22%2C%22lastName%22%3A%22Obarska-Kosi%5Cu0144ska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20R.%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beata%22%2C%22lastName%22%3A%22Turo%5Cu0148ov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacomina%20Krijnse%22%2C%22lastName%22%3A%22Locker%22%7D%5D%2C%22abstractNote%22%3A%22Due%20to%20its%20asymmetric%20shape%2C%20size%20and%20compactness%2C%20the%20structure%20of%20the%20infectious%20mature%20virus%20%28MV%29%20of%20vaccinia%20virus%20%28VACV%29%2C%20the%20best-studied%20poxvirus%2C%20remains%20poorly%20understood.%20Instead%2C%20subviral%20particles%2C%20in%20particular%20membrane-free%20viral%20cores%2C%20have%20been%20studied%20with%20cryo-electron%20microscopy.%20Here%2C%20we%20compared%20viral%20cores%20obtained%20by%20detergent%20stripping%20of%20MVs%20with%20cores%20in%20the%20cellular%20cytoplasm%2C%20early%20in%20infection.%20We%20focused%20on%20the%20prominent%20palisade%20layer%20on%20the%20core%20surface%2C%20combining%20cryo-electron%20tomography%2C%20subtomogram%20averaging%20and%20AlphaFold2%20structure%20prediction.%20We%20showed%20that%20the%20palisade%20is%20composed%20of%20densely%20packed%20trimers%20of%20the%20major%20core%20protein%20A10.%20Trimers%20display%20a%20random%20order%20and%20their%20classification%20indicates%20structural%20flexibility.%20A10%20on%20cytoplasmic%20cores%20is%20organized%20in%20a%20similar%20manner%2C%20indicating%20that%20the%20structures%20obtained%20in%20vitro%20are%20physiologically%20relevant.%20We%20discuss%20our%20results%20in%20the%20context%20of%20the%20VACV%20replicative%20cycle%2C%20and%20the%20assembly%20and%20disassembly%20of%20the%20infectious%20MV.%22%2C%22date%22%3A%222024-02-05%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41594-024-01218-5%22%2C%22ISSN%22%3A%221545-9985%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222024-02-06T16%3A05%3A20Z%22%7D%7D%2C%7B%22key%22%3A%22RGW3QGNT%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Williams%20et%20al.%22%2C%22parsedDate%22%3A%222023-01-18%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWilliams%2C%20Harry%20M.%2C%20Sigurdur%20R.%20Thorkelsson%2C%20Dominik%20Vogel%2C%20Morlin%20Milewski%2C%20Carola%20Busch%2C%20Stephen%20Cusack%2C%20Kay%20Gr%26%23xFC%3Bnewald%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20and%20Maria%20Rosenthal.%202023.%20%26%23x201C%3BStructural%20Insights%20into%20Viral%20Genome%20Replication%20by%20the%20Severe%20Fever%20with%20Thrombocytopenia%20Syndrome%20Virus%20L%20Protein.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2C%20January%2C%20gkac1249.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkac1249%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkac1249%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20insights%20into%20viral%20genome%20replication%20by%20the%20severe%20fever%20with%20thrombocytopenia%20syndrome%20virus%20L%20protein%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harry%20M.%22%2C%22lastName%22%3A%22Williams%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20R.%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominik%22%2C%22lastName%22%3A%22Vogel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Morlin%22%2C%22lastName%22%3A%22Milewski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carola%22%2C%22lastName%22%3A%22Busch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%22%2C%22lastName%22%3A%22Cusack%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Rosenthal%22%7D%5D%2C%22abstractNote%22%3A%22Severe%20fever%20with%20thrombocytopenia%20syndrome%20virus%20%28SFTSV%29%20is%20a%20phenuivirus%20that%20has%20rapidly%20become%20endemic%20in%20several%20East%20Asian%20countries.%20The%20large%20%28L%29%20protein%20of%20SFTSV%2C%20which%20includes%20the%20RNA-dependent%20RNA%20polymerase%20%28RdRp%29%2C%20is%20responsible%20for%20catalysing%20viral%20genome%20replication%20and%20transcription.%20Here%2C%20we%20present%205%20cryo-electron%20microscopy%20%28cryo-EM%29%20structures%20of%20the%20L%20protein%20in%20several%20states%20of%20the%20genome%20replication%20process%2C%20from%20pre-initiation%20to%20late-stage%20elongation%2C%20at%20a%20resolution%20of%20up%20to%202.6%20%5Cu00c5.%20We%20identify%20how%20the%20L%20protein%20binds%20the%205%27%20viral%20RNA%20in%20a%20hook-like%20conformation%20and%20show%20how%20the%20distal%205%27%20and%203%27%20RNA%20ends%20form%20a%20duplex%20positioning%20the%203%27%20RNA%20terminus%20in%20the%20RdRp%20active%20site%20ready%20for%20initiation.%20We%20also%20observe%20the%20L%20protein%20stalled%20in%20the%20early%20and%20late%20stages%20of%20elongation%20with%20the%20RdRp%20core%20accommodating%20a%2010-bp%20product-template%20duplex.%20This%20duplex%20ultimately%20splits%20with%20the%20template%20binding%20to%20a%20designated%203%27%20secondary%20binding%20site.%20The%20structural%20data%20and%20observations%20are%20complemented%20by%20in%20vitro%20biochemical%20and%20cell-based%20mini-replicon%20assays.%20Altogether%2C%20our%20data%20provide%20novel%20key%20insights%20into%20the%20mechanism%20of%20viral%20genome%20replication%20by%20the%20SFTSV%20L%20protein%20and%20will%20aid%20drug%20development%20against%20segmented%20negative-strand%20RNA%20viruses.%22%2C%22date%22%3A%222023-01-18%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkac1249%22%2C%22ISSN%22%3A%221362-4962%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222023-02-13T14%3A33%3A36Z%22%7D%7D%2C%7B%22key%22%3A%22SHSFAQDW%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Villalta%20et%20al.%22%2C%22parsedDate%22%3A%222022-07-28%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVillalta%2C%20Alejandro%2C%20Alain%20Schmitt%2C%20Leandro%20F%20Estrozi%2C%20Emmanuelle%20RJ%20Quemin%2C%20Jean-Marie%20Alempic%2C%20Audrey%20Lartigue%2C%20Vojt%26%23x11B%3Bch%20Pra%26%23x17E%3B%26%23xE1%3Bk%2C%20et%20al.%202022.%20%26%23x201C%3BThe%20Giant%20Mimivirus%201.2%20Mb%20Genome%20Is%20Elegantly%20Organized%20into%20a%2030%20Nm%20Diameter%20Helical%20Protein%20Shield.%26%23x201D%3B%20Edited%20by%20Adam%20Frost.%20%3Ci%3EELife%3C%5C%2Fi%3E%2011%20%28July%29%3Ae77607.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.77607%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.77607%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20giant%20mimivirus%201.2%20Mb%20genome%20is%20elegantly%20organized%20into%20a%2030%20nm%20diameter%20helical%20protein%20shield%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alejandro%22%2C%22lastName%22%3A%22Villalta%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alain%22%2C%22lastName%22%3A%22Schmitt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Leandro%20F%22%2C%22lastName%22%3A%22Estrozi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20RJ%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Marie%22%2C%22lastName%22%3A%22Alempic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Audrey%22%2C%22lastName%22%3A%22Lartigue%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22Pra%5Cu017e%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daven%22%2C%22lastName%22%3A%22Vasishtan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Agathe%20MG%22%2C%22lastName%22%3A%22Colmant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Flora%20A%22%2C%22lastName%22%3A%22Honor%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chantal%22%2C%22lastName%22%3A%22Abergel%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Adam%22%2C%22lastName%22%3A%22Frost%22%7D%5D%2C%22abstractNote%22%3A%22Mimivirus%20is%20the%20prototype%20of%20the%20Mimiviridae%20family%20of%20giant%20dsDNA%20viruses.%20Little%20is%20known%20about%20the%20organization%20of%20the%201.2%20Mb%20genome%20inside%20the%20membrane-limited%20nucleoid%20filling%20the%20~0.5%20%5Cu00b5m%20icosahedral%20capsids.%20Cryo-electron%20microscopy%2C%20cryo-electron%20tomography%20and%20proteomics%20revealed%20that%20it%20is%20encased%20into%20a%20~30%20nm%20diameter%20helical%20protein%20shell%20surprisingly%20composed%20of%20two%20GMC-type%20oxidoreductases%2C%20which%20also%20form%20the%20glycosylated%20fibrils%20decorating%20the%20capsid.%20The%20genome%20is%20arranged%20in%205-%20or%206-start%20left-handed%20super-helices%2C%20with%20each%20DNA-strand%20lining%20the%20central%20channel.%20This%20luminal%20channel%20of%20the%20nucleoprotein%20fiber%20is%20wide%20enough%20to%20accommodate%20oxidative%20stress%20proteins%20and%20RNA%20polymerase%20subunits%20identified%20by%20proteomics.%20Such%20elegant%20supramolecular%20organization%20would%20represent%20a%20remarkable%20evolutionary%20strategy%20for%20packaging%20and%20protecting%20the%20genome%2C%20in%20a%20state%20ready%20for%20immediate%20transcription%20upon%20unwinding%20in%20the%20host%20cytoplasm.%20The%20parsimonious%20use%20of%20the%20same%20protein%20in%20two%20unrelated%20substructures%20of%20the%20virion%20is%20unexpected%20for%20a%20giant%20virus%20with%20thousand%20genes%20at%20its%20disposal.%22%2C%22date%22%3A%222022-07-28%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.7554%5C%2FeLife.77607%22%2C%22ISSN%22%3A%222050-084X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.77607%22%2C%22collections%22%3A%5B%22YCWTNSJP%22%5D%2C%22dateModified%22%3A%222022-08-24T06%3A42%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22WV4AQTZB%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kouba%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-02%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKouba%2C%20Tomas%2C%20Dominik%20Vogel%2C%20Sigurdur%20R.%20Thorkelsson%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20Harry%20M.%20Williams%2C%20Morlin%20Milewski%2C%20Carola%20Busch%2C%20et%20al.%202021.%20%26%23x201C%3BConformational%20Changes%20in%20Lassa%20Virus%20L%20Protein%20Associated%20with%20Promoter%20Binding%20and%20RNA%20Synthesis%20Activity.%26%23x201D%3B%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2012%20%281%29%3A%207018.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-021-27305-5%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-021-27305-5%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Conformational%20changes%20in%20Lassa%20virus%20L%20protein%20associated%20with%20promoter%20binding%20and%20RNA%20synthesis%20activity%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomas%22%2C%22lastName%22%3A%22Kouba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominik%22%2C%22lastName%22%3A%22Vogel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20R.%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harry%20M.%22%2C%22lastName%22%3A%22Williams%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Morlin%22%2C%22lastName%22%3A%22Milewski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carola%22%2C%22lastName%22%3A%22Busch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephan%22%2C%22lastName%22%3A%22G%5Cu00fcnther%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Rosenthal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%22%2C%22lastName%22%3A%22Cusack%22%7D%5D%2C%22abstractNote%22%3A%22Lassa%20virus%20is%20endemic%20in%20West%20Africa%20and%20can%20cause%20severe%20hemorrhagic%20fever.%20The%20viral%20L%20protein%20transcribes%20and%20replicates%20the%20RNA%20genome%20via%20its%20RNA-dependent%20RNA%20polymerase%20activity.%20Here%2C%20we%20present%20nine%20cryo-EM%20structures%20of%20the%20L%20protein%20in%20the%20apo-%2C%20promoter-bound%20pre-initiation%20and%20active%20RNA%20synthesis%20states.%20We%20characterize%20distinct%20binding%20pockets%20for%20the%20conserved%203%5Cu2019%20and%205%5Cu2019%20promoter%20RNAs%20and%20show%20how%20full-promoter%20binding%20induces%20a%20distinct%20pre-initiation%20conformation.%20In%20the%20apo-%20and%20early%20elongation%20states%2C%20the%20endonuclease%20is%20inhibited%20by%20two%20distinct%20L%20protein%20peptides%2C%20whereas%20in%20the%20pre-initiation%20state%20it%20is%20uninhibited.%20In%20the%20early%20elongation%20state%2C%20a%20template-product%20duplex%20is%20bound%20in%20the%20active%20site%20cavity%20together%20with%20an%20incoming%20non-hydrolysable%20nucleotide%20and%20the%20full%20C-terminal%20region%20of%20the%20L%20protein%2C%20including%20the%20putative%20cap-binding%20domain%2C%20is%20well-ordered.%20These%20data%20advance%20our%20mechanistic%20understanding%20of%20how%20this%20flexible%20and%20multifunctional%20molecular%20machine%20is%20activated.%22%2C%22date%22%3A%222021-12-02%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-021-27305-5%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-021-27305-5%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A44%3A15Z%22%7D%7D%2C%7B%22key%22%3A%2276JN87AV%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Meier%20et%20al.%22%2C%22parsedDate%22%3A%222021-08-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMeier%2C%20Kristina%2C%20Sigurdur%20R.%20Thorkelsson%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20and%20Maria%20Rosenthal.%202021.%20%26%23x201C%3BHantavirus%20Replication%20Cycle%26%23x2014%3BAn%20Updated%20Structural%20Virology%20Perspective.%26%23x201D%3B%20%3Ci%3EViruses%3C%5C%2Fi%3E%2013%20%288%29%3A%201561.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fv13081561%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fv13081561%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Hantavirus%20Replication%20Cycle%5Cu2014An%20Updated%20Structural%20Virology%20Perspective%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kristina%22%2C%22lastName%22%3A%22Meier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20R.%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Rosenthal%22%7D%5D%2C%22abstractNote%22%3A%22Hantaviruses%20infect%20a%20wide%20range%20of%20hosts%20including%20insectivores%20and%20rodents%20and%20can%20also%20cause%20zoonotic%20infections%20in%20humans%2C%20which%20can%20lead%20to%20severe%20disease%20with%20possible%20fatal%20outcomes.%20Hantavirus%20outbreaks%20are%20usually%20linked%20to%20the%20population%20dynamics%20of%20the%20host%20animals%20and%20their%20habitats%20being%20in%20close%20proximity%20to%20humans%2C%20which%20is%20becoming%20increasingly%20important%20in%20a%20globalized%20world.%20Currently%20there%20is%20neither%20an%20approved%20vaccine%20nor%20a%20specific%20and%20effective%20antiviral%20treatment%20available%20for%20use%20in%20humans.%20Hantaviruses%20belong%20to%20the%20order%20Bunyavirales%20with%20a%20tri-segmented%20negative-sense%20RNA%20genome.%20They%20encode%20only%20five%20viral%20proteins%20and%20replicate%20and%20transcribe%20their%20genome%20in%20the%20cytoplasm%20of%20infected%20cells.%20However%2C%20many%20details%20of%20the%20viral%20amplification%20cycle%20are%20still%20unknown.%20In%20recent%20years%2C%20structural%20biology%20methods%20such%20as%20cryo-electron%20tomography%2C%20cryo-electron%20microscopy%2C%20and%20crystallography%20have%20contributed%20essentially%20to%20our%20understanding%20of%20virus%20entry%20by%20membrane%20fusion%20as%20well%20as%20genome%20encapsidation%20by%20the%20nucleoprotein.%20In%20this%20review%2C%20we%20provide%20an%20update%20on%20the%20hantavirus%20replication%20cycle%20with%20a%20special%20focus%20on%20structural%20virology%20aspects.%22%2C%22date%22%3A%222021-08-06%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fv13081561%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F1999-4915%5C%2F13%5C%2F8%5C%2F1561%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222022-06-07T11%3A38%3A27Z%22%7D%7D%2C%7B%22key%22%3A%22HZHNA5YW%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Vogel%20et%20al.%22%2C%22parsedDate%22%3A%222020-06-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVogel%2C%20Dominik%2C%20Sigurdur%20Rafn%20Thorkelsson%2C%20Emmanuelle%20R%20J%20Quemin%2C%20Kristina%20Meier%2C%20Tomas%20Kouba%2C%20Nadja%20Gogrefe%2C%20Carola%20Busch%2C%20et%20al.%202020.%20%26%23x201C%3BStructural%20and%20Functional%20Characterization%20of%20the%20Severe%20Fever%20with%20Thrombocytopenia%20Syndrome%20Virus%20L%20Protein.%26%23x201D%3B%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2048%20%2810%29%3A%205749%26%23x2013%3B65.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkaa253%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkaa253%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20and%20functional%20characterization%20of%20the%20severe%20fever%20with%20thrombocytopenia%20syndrome%20virus%20L%20protein%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominik%22%2C%22lastName%22%3A%22Vogel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigurdur%20Rafn%22%2C%22lastName%22%3A%22Thorkelsson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R%20J%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kristina%22%2C%22lastName%22%3A%22Meier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomas%22%2C%22lastName%22%3A%22Kouba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nadja%22%2C%22lastName%22%3A%22Gogrefe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carola%22%2C%22lastName%22%3A%22Busch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophia%22%2C%22lastName%22%3A%22Reindl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephan%22%2C%22lastName%22%3A%22G%5Cu00fcnther%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stephen%22%2C%22lastName%22%3A%22Cusack%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Rosenthal%22%7D%5D%2C%22abstractNote%22%3A%22The%20Bunyavirales%20order%20contains%20several%20emerging%20viruses%20with%20high%20epidemic%20potential%2C%20including%20Severe%20fever%20with%20thrombocytopenia%20syndrome%20virus%20%28SFTSV%29.%20The%20lack%20of%20medical%20countermeasures%2C%20such%20as%20vaccines%20and%20antivirals%2C%20is%20a%20limiting%20factor%20for%20the%20containment%20of%20any%20virus%20outbreak.%20To%20develop%20such%20antivirals%20a%20profound%20understanding%20of%20the%20viral%20replication%20process%20is%20essential.%20The%20L%20protein%20of%20bunyaviruses%20is%20a%20multi-functional%20and%20multi-domain%20protein%20performing%20both%20virus%20transcription%20and%20genome%20replication%20and%2C%20therefore%2C%20is%20an%20ideal%20drug%20target.%20We%20established%20expression%20and%20purification%20procedures%20for%20the%20full-length%20L%20protein%20of%20SFTSV.%20By%20combining%20single-particle%20electron%20cryo-microscopy%20and%20X-ray%20crystallography%2C%20we%20obtained%203D%20models%20covering%20%5Cu223c70%25%20of%20the%20SFTSV%20L%20protein%20in%20the%20apo-conformation%20including%20the%20polymerase%20core%20region%2C%20the%20endonuclease%20and%20the%20cap-binding%20domain.%20We%20compared%20this%20first%20L%20structure%20of%20the%20Phenuiviridae%20family%20to%20the%20structures%20of%20La%20Crosse%20peribunyavirus%20L%20protein%20and%20influenza%20orthomyxovirus%20polymerase.%20Together%20with%20a%20comprehensive%20biochemical%20characterization%20of%20the%20distinct%20functions%20of%20SFTSV%20L%20protein%2C%20this%20work%20provides%20a%20solid%20framework%20for%20future%20structural%20and%20functional%20studies%20of%20L%20protein%5Cu2013RNA%20interactions%20and%20the%20development%20of%20antiviral%20strategies%20against%20this%20group%20of%20emerging%20human%20pathogens.%22%2C%22date%22%3A%22June%204%2C%202020%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkaa253%22%2C%22ISSN%22%3A%220305-1048%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkaa253%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A22%3A05Z%22%7D%7D%2C%7B%22key%22%3A%2287IFSNC4%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222020%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.J.%2C%20Emily%20A.%20Machala%2C%20Benjamin%20Vollmer%2C%20Vojt%26%23x11B%3Bch%20Pra%26%23x17E%3B%26%23xE1%3Bk%2C%20Daven%20Vasishtan%2C%20Rene%20Rosch%2C%20Michael%20Grange%2C%20Linda%20E.%20Franken%2C%20Lindsay%20A.%20Baker%2C%20and%20Kay%20Gr%26%23xFC%3Bnewald.%202020.%20%26%23x201C%3BCellular%20Electron%20Cryo-Tomography%20to%20Study%20Virus-Host%20Interactions.%26%23x201D%3B%20%3Ci%3EAnnual%20Review%20of%20Virology%3C%5C%2Fi%3E%207%20%281%29%3A%20239%26%23x2013%3B62.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1146%5C%2Fannurev-virology-021920-115935%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1146%5C%2Fannurev-virology-021920-115935%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cellular%20Electron%20Cryo-Tomography%20to%20Study%20Virus-Host%20Interactions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emily%20A.%22%2C%22lastName%22%3A%22Machala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benjamin%22%2C%22lastName%22%3A%22Vollmer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vojt%5Cu011bch%22%2C%22lastName%22%3A%22Pra%5Cu017e%5Cu00e1k%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daven%22%2C%22lastName%22%3A%22Vasishtan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rene%22%2C%22lastName%22%3A%22Rosch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Grange%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Linda%20E.%22%2C%22lastName%22%3A%22Franken%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lindsay%20A.%22%2C%22lastName%22%3A%22Baker%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kay%22%2C%22lastName%22%3A%22Gr%5Cu00fcnewald%22%7D%5D%2C%22abstractNote%22%3A%22Viruses%20are%20obligatory%20intracellular%20parasites%20that%20reprogram%20host%20cells%20upon%20infection%20to%20produce%20viral%20progeny.%20Here%2C%20we%20review%20recent%20structural%20insights%20into%20virus-host%20interactions%20in%20bacteria%2C%20archaea%2C%20and%20eukaryotes%20unveiled%20by%20cellular%20electron%20cryo-tomography%20%28cryoET%29.%20This%20advanced%20three-dimensional%20imaging%20technique%20of%20vitreous%20samples%20in%20near-native%20state%20has%20matured%20over%20the%20past%20two%20decades%20and%20proven%20powerful%20in%20revealing%20molecular%20mechanisms%20underlying%20viral%20replication.%20Initial%20studies%20were%20restricted%20to%20cell%20peripheries%20and%20typically%20focused%20on%20early%20infection%20steps%2C%20analyzing%20surface%20proteins%20and%20viral%20entry.%20Recent%20developments%20including%20cryo-thinning%20techniques%2C%20phase-plate%20imaging%2C%20and%20correlative%20approaches%20have%20been%20instrumental%20in%20also%20targeting%20rare%20events%20inside%20infected%20cells.%20When%20combined%20with%20advances%20in%20dedicated%20image%20analyses%20and%20processing%20methods%2C%20details%20of%20virus%20assembly%20and%20egress%20at%20%28sub%29nanometer%20resolution%20were%20uncovered.%20Altogether%2C%20we%20provide%20a%20historical%20and%20technical%20perspective%20and%20discuss%20future%20directions%20and%20impacts%20of%20cryoET%20for%20integrative%20structural%20cell%20biology%20analyses%20of%20viruses.%22%2C%22date%22%3A%222020%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1146%5C%2Fannurev-virology-021920-115935%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1146%5C%2Fannurev-virology-021920-115935%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-20T12%3A03%3A56Z%22%7D%7D%2C%7B%22key%22%3A%22TM9ZSXNH%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222019-04-17%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20E.%20R.%2C%20S.%20Corroyer-Dulmont%2C%20A.%20Baskaran%2C%20E.%20Penard%2C%20A.%20D.%20Gazi%2C%20E.%20Christo-Foroux%2C%20P.%20Walther%2C%20C.%20Abergel%2C%20and%20J.%20Krijnse-Locker.%202019.%20%26%23x201C%3BComplex%20Membrane%20Remodeling%20during%20Virion%20Assembly%20of%20the%2030%2C000-Year-Old%20Mollivirus%20Sibericum.%26%23x201D%3B%20%3Ci%3EJournal%20of%20Virology%3C%5C%2Fi%3E%2C%20April.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.00388-19%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.00388-19%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Complex%20Membrane%20Remodeling%20during%20Virion%20Assembly%20of%20the%2030%2C000-Year-Old%20Mollivirus%20Sibericum%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%20R.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Corroyer-Dulmont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Baskaran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Penard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20D.%22%2C%22lastName%22%3A%22Gazi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Christo-Foroux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Walther%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Abergel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%22%2C%22lastName%22%3A%22Krijnse-Locker%22%7D%5D%2C%22abstractNote%22%3A%22Since%20the%20first%20giant%20virus%20Mimivirus%20was%20identified%2C%20other%20giant%20representatives%20are%20isolated%20regularly%20around%20the%20world%5Cnand%20appear%20to%20be%20unique%20in%20several%20aspects.%20They%20belong%20to%20at%20least%20four%20viral%20families%2C%5Cnand%20the%20ways%20they%20interact%20with%20their%20hosts%20...%22%2C%22date%22%3A%222019-4-17%22%2C%22language%22%3A%22EN%22%2C%22DOI%22%3A%2210.1128%5C%2FJVI.00388-19%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.00388-19%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A26%3A58Z%22%7D%7D%2C%7B%22key%22%3A%22UESKPWMA%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222018-06-08%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.%2C%20Simon%20Corroyer-Dulmont%2C%20and%20Jacomine%20Krijnse-Locker.%202018.%20%26%23x201C%3BEntry%20and%20Disassembly%20of%20Large%20DNA%20Viruses%3A%20Electron%20Microscopy%20Leads%20the%20Way.%26%23x201D%3B%20%3Ci%3EJournal%20of%20Molecular%20Biology%3C%5C%2Fi%3E%20430%20%2812%29%3A%201714%26%23x2013%3B24.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jmb.2018.04.019%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jmb.2018.04.019%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Entry%20and%20Disassembly%20of%20Large%20DNA%20Viruses%3A%20Electron%20Microscopy%20Leads%20the%20Way%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simon%22%2C%22lastName%22%3A%22Corroyer-Dulmont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacomine%22%2C%22lastName%22%3A%22Krijnse-Locker%22%7D%5D%2C%22abstractNote%22%3A%22Nucleocytoplasmic%20large%20DNA%20viruses%20are%20a%20steadily%20growing%20group%20of%20viruses%20that%20infect%20a%20wide%20range%20of%20hosts%20and%20are%20characterized%20by%20large%20particle%20dimensions%20and%20genome%20sizes.%20Understanding%20how%20they%20enter%20into%20the%20host%20cell%20and%20deliver%20their%20genome%20in%20the%20cytoplasm%20is%20therefore%20particularly%20intriguing.%20Here%2C%20we%20review%20the%20current%20knowledge%20on%20the%20entry%20of%20two%20of%20the%20best-characterized%20nucleocytoplasmic%20large%20DNA%20viruses%3A%20the%20poxvirus%20Vaccinia%20virus%20%28VACV%29%20and%20the%20giant%20virus%20Mimivirus.%20While%20previous%20studies%20on%20VACV%20had%20proposed%20both%20direct%20fusion%20at%20the%20plasma%20membrane%20and%20endocytosis%20as%20entry%20routes%2C%20more%20recent%20biochemical%20and%20morphological%20data%20argue%20for%20macropinocytosis%20as%20well.%20Notably%2C%20direct%20imaging%20by%20electron%20microscopy%20%28EM%29%20also%20supported%20the%20existence%20of%20parallel%20ways%20of%20entry%20for%20VACV.%20Instead%2C%20all%20the%20giant%20viruses%20studied%20so%20far%20only%20enter%20cells%20by%20phagocytosis%20as%20observed%20by%20EM%2C%20and%20we%20discuss%20the%20mechanisms%20for%20opening%20of%20the%20particle%2C%20fusion%20of%20the%20viral%20and%20phagosomal%20membranes%20and%20genome%20delivery%20via%20a%20unique%20portal%2C%20specific%20for%20each%20giant%20virus.%20VACV%20core%20uncoating%2C%20in%20contrast%2C%20remains%20a%20morphologically%20ill-defined%20process.%20We%20argue%20that%20correlated%20light%20and%20electron%20microscopy%20methods%20are%20required%20to%20study%20VACV%20entry%20and%20uncoating%20in%20a%20direct%20and%20systematic%20manner.%20Such%20EM%20studies%20should%20also%20address%20whether%20entry%20of%20single%20particles%20and%20viral%20aggregates%20is%20different%20and%20thus%20provide%20an%20explanation%20for%20the%20different%20modes%20of%20entry%20described%20in%20the%20literature.%22%2C%22date%22%3A%22June%208%2C%202018%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jmb.2018.04.019%22%2C%22ISSN%22%3A%220022-2836%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0022283618302729%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A31%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22ZQKR4ICB%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Badders%20et%20al.%22%2C%22parsedDate%22%3A%222018-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBadders%2C%20Nisha%20M.%2C%20Ane%20Korff%2C%20Helen%20C.%20Miranda%2C%20Pradeep%20K.%20Vuppala%2C%20Rebecca%20B.%20Smith%2C%20Brett%20J.%20Winborn%2C%20Emmanuelle%20R.%20Quemin%2C%20et%20al.%202018.%20%26%23x201C%3BSelective%20Modulation%20of%20the%20Androgen%20Receptor%20AF2%20Domain%20Rescues%20Degeneration%20in%20Spinal%20Bulbar%20Muscular%20Atrophy.%26%23x201D%3B%20%3Ci%3ENature%20Medicine%3C%5C%2Fi%3E%2024%20%284%29%3A%20427%26%23x2013%3B37.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnm.4500%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fnm.4500%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Selective%20modulation%20of%20the%20androgen%20receptor%20AF2%20domain%20rescues%20degeneration%20in%20spinal%20bulbar%20muscular%20atrophy%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nisha%20M.%22%2C%22lastName%22%3A%22Badders%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ane%22%2C%22lastName%22%3A%22Korff%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helen%20C.%22%2C%22lastName%22%3A%22Miranda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pradeep%20K.%22%2C%22lastName%22%3A%22Vuppala%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%20B.%22%2C%22lastName%22%3A%22Smith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brett%20J.%22%2C%22lastName%22%3A%22Winborn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bryce%20L.%22%2C%22lastName%22%3A%22Sopher%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Dearman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%22%2C%22lastName%22%3A%22Messing%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nam%20Chul%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Moore%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Brian%20D.%22%2C%22lastName%22%3A%22Freibaum%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anderson%20P.%22%2C%22lastName%22%3A%22Kanagaraj%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Baochang%22%2C%22lastName%22%3A%22Fan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Heather%22%2C%22lastName%22%3A%22Tillman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ping-Chung%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yingzhe%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Burgess%20B.%20Freeman%22%2C%22lastName%22%3A%22Iii%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yimei%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hong%20Joo%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Albert%20R.%22%2C%22lastName%22%3A%22La%20Spada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20Paul%22%2C%22lastName%22%3A%22Taylor%22%7D%5D%2C%22abstractNote%22%3A%22A%20preclinical%20therapy%20to%20treat%20neurodegeneration%20is%20developed%20that%20selectively%20targets%20the%20AF-2%20domain%20of%20the%20androgen%20receptor%20while%20sparing%20other%20functions%20of%20this%20receptor.%22%2C%22date%22%3A%222018-04%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fnm.4500%22%2C%22ISSN%22%3A%221546-170X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fnm.4500%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A29%3A43Z%22%7D%7D%2C%7B%22key%22%3A%22XN75XUIK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222016-09-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.%20J.%2C%20Petr%20Chlanda%2C%20Martin%20Sachse%2C%20Patrick%20Forterre%2C%20David%20Prangishvili%2C%20and%20Mart%20Krupovic.%202016.%20%26%23x201C%3BEukaryotic-Like%20Virus%20Budding%20in%20Archaea.%26%23x201D%3B%20%3Ci%3EMBio%3C%5C%2Fi%3E%2C%20September.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmBio.01439-16%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FmBio.01439-16%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Eukaryotic-Like%20Virus%20Budding%20in%20Archaea%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Petr%22%2C%22lastName%22%3A%22Chlanda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Sachse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Forterre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%5D%2C%22abstractNote%22%3A%22The%20replication%20of%20enveloped%20viruses%20has%20been%20extensively%20studied%20in%20eukaryotes%20but%5Cnhas%20remained%20unexplored%20for%20enveloped%20viruses%20infecting%20bacteria%20and%20archaea.%20Here%2C%5Cnwe%20provide%20a%20sequential%20view%20on%20the%20assembly%20and%20egress%20of%20SSV1%2C%20a%20prototypic%20archaeal%5Cn...%22%2C%22date%22%3A%222016-9-13%22%2C%22language%22%3A%22EN%22%2C%22DOI%22%3A%2210.1128%5C%2FmBio.01439-16%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FmBio.01439-16%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A32%3A50Z%22%7D%7D%2C%7B%22key%22%3A%22HJHVEJW5%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Rensen%20et%20al.%22%2C%22parsedDate%22%3A%222016-03-01%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERensen%2C%20Elena%20Ilka%2C%20Tomohiro%20Mochizuki%2C%20Emmanuelle%20Quemin%2C%20Stefan%20Schouten%2C%20Mart%20Krupovic%2C%20and%20David%20Prangishvili.%202016.%20%26%23x201C%3BA%20Virus%20of%20Hyperthermophilic%20Archaea%20with%20a%20Unique%20Architecture%20among%20DNA%20Viruses.%26%23x201D%3B%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%3C%5C%2Fi%3E%20113%20%289%29%3A%202478%26%23x2013%3B83.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1518929113%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.1518929113%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20virus%20of%20hyperthermophilic%20archaea%20with%20a%20unique%20architecture%20among%20DNA%20viruses%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%20Ilka%22%2C%22lastName%22%3A%22Rensen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomohiro%22%2C%22lastName%22%3A%22Mochizuki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefan%22%2C%22lastName%22%3A%22Schouten%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%5D%2C%22abstractNote%22%3A%22Viruses%20package%20their%20genetic%20material%20in%20diverse%20ways.%20Most%20known%20strategies%20include%20encapsulation%20of%20nucleic%20acids%20into%20spherical%20or%20filamentous%20virions%20with%20icosahedral%20or%20helical%20symmetry%2C%20respectively.%20Filamentous%20viruses%20with%20dsDNA%20genomes%20are%20currently%20associated%20exclusively%20with%20Archaea.%20Here%2C%20we%20describe%20a%20filamentous%20hyperthermophilic%20archaeal%20virus%2C%20Pyrobaculum%20filamentous%20virus%201%20%28PFV1%29%2C%20with%20a%20type%20of%20virion%20organization%20not%20previously%20observed%20in%20DNA%20viruses.%20The%20PFV1%20virion%2C%20400%20%5Cu00b1%2020%20%5Cu00d7%2032%20%5Cu00b1%203%20nm%2C%20contains%20an%20envelope%20and%20an%20inner%20core%20consisting%20of%20two%20structural%20units%3A%20a%20rod-shaped%20helical%20nucleocapsid%20formed%20of%20two%2014-kDa%20major%20virion%20proteins%20and%20a%20nucleocapsid-encompassing%20protein%20sheath%20composed%20of%20a%20single%20major%20virion%20protein%20of%2018%20kDa.%20The%20virion%20organization%20of%20PFV1%20is%20superficially%20similar%20to%20that%20of%20negative-sense%20RNA%20viruses%20of%20the%20family%20Filoviridae%2C%20including%20Ebola%20virus%20and%20Marburg%20virus.%20The%20linear%20dsDNA%20of%20PFV1%20carries%2017%2C714%20bp%2C%20including%2060-bp-long%20terminal%20inverted%20repeats%2C%20and%20contains%2039%20predicted%20ORFs%2C%20most%20of%20which%20do%20not%20show%20similarities%20to%20sequences%20in%20public%20databases.%20PFV1%20is%20a%20lytic%20virus%20that%20completely%20disrupts%20the%20host%20cell%20membrane%20at%20the%20end%20of%20the%20infection%20cycle.%22%2C%22date%22%3A%222016%5C%2F03%5C%2F01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.1518929113%22%2C%22ISSN%22%3A%220027-8424%2C%201091-6490%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.pnas.org%5C%2Fcontent%5C%2F113%5C%2F9%5C%2F2478%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A35%3A48Z%22%7D%7D%2C%7B%22key%22%3A%2293QAWDVF%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222015-09-09%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.%20J.%2C%20Maija%20K.%20Pietil%26%23xE4%3B%2C%20Hanna%20M.%20Oksanen%2C%20Patrick%20Forterre%2C%20W.%20Irene%20C.%20Rijpstra%2C%20Stefan%20Schouten%2C%20Dennis%20H.%20Bamford%2C%20David%20Prangishvili%2C%20and%20Mart%20Krupovic.%202015.%20%26%23x201C%3BSulfolobus%20Spindle-Shaped%20Virus%201%20Contains%20Glycosylated%20Capsid%20Proteins%2C%20a%20Cellular%20Chromatin%20Protein%2C%20and%20Host-Derived%20Lipids.%26%23x201D%3B%20%3Ci%3EJournal%20of%20Virology%3C%5C%2Fi%3E%2C%20September.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.02270-15%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.02270-15%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Sulfolobus%20Spindle-Shaped%20Virus%201%20Contains%20Glycosylated%20Capsid%20Proteins%2C%20a%20Cellular%20Chromatin%20Protein%2C%20and%20Host-Derived%20Lipids%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maija%20K.%22%2C%22lastName%22%3A%22Pietil%5Cu00e4%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hanna%20M.%22%2C%22lastName%22%3A%22Oksanen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Forterre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22W.%20Irene%20C.%22%2C%22lastName%22%3A%22Rijpstra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefan%22%2C%22lastName%22%3A%22Schouten%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dennis%20H.%22%2C%22lastName%22%3A%22Bamford%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%5D%2C%22abstractNote%22%3A%22Geothermal%20and%20hypersaline%20environments%20are%20rich%20in%20virus-like%20particles%2C%20among%20which%5Cnspindle-shaped%20morphotypes%20dominate.%20Currently%2C%20viruses%20with%20spindle-%20or%20lemon-shaped%5Cnvirions%20are%20exclusive%20to%20Archaea%20and%20belong%20to%20two%20distinct%20viral%20families.%20...%22%2C%22date%22%3A%222015-9-9%22%2C%22language%22%3A%22EN%22%2C%22DOI%22%3A%2210.1128%5C%2FJVI.02270-15%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.02270-15%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A37%3A16Z%22%7D%7D%2C%7B%22key%22%3A%22B2WVKCUM%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20and%20Quax%22%2C%22parsedDate%22%3A%222015%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.%20J.%2C%20and%20Tessa%20E.%20F.%20Quax.%202015.%20%26%23x201C%3BArchaeal%20Viruses%20at%20the%20Cell%20Envelope%3A%20Entry%20and%20Egress.%26%23x201D%3B%20%3Ci%3EFrontiers%20in%20Microbiology%3C%5C%2Fi%3E%206%3A552.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2015.00552%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffmicb.2015.00552%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Archaeal%20viruses%20at%20the%20cell%20envelope%3A%20entry%20and%20egress%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tessa%20E.%20F.%22%2C%22lastName%22%3A%22Quax%22%7D%5D%2C%22abstractNote%22%3A%22The%20cell%20envelope%20represents%20the%20main%20line%20of%20host%20defense%20that%20viruses%20encounter%20on%20their%20way%20from%20one%20cell%20to%20another.%20The%20cytoplasmic%20membrane%20in%20general%20is%20a%20physical%20barrier%20that%20needs%20to%20be%20crossed%20both%20upon%20viral%20entry%20and%20exit.%20Therefore%2C%20viruses%20from%20the%20three%20domains%20of%20life%20employ%20a%20wide%20range%20of%20strategies%20for%20perforation%20of%20the%20cell%20membrane%2C%20each%20adapted%20to%20the%20cell%20surface%20environment%20of%20their%20host.%20Here%2C%20we%20review%20recent%20insights%20on%20entry%20and%20egress%20mechanisms%20of%20viruses%20infecting%20archaea.%20Due%20to%20the%20unique%20nature%20of%20the%20archaeal%20cell%20envelope%2C%20these%20particular%20viruses%20exhibit%20novel%20and%20unexpected%20mechanisms%20to%20traverse%20the%20cellular%20membrane.%22%2C%22date%22%3A%222015%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.3389%5C%2Ffmicb.2015.00552%22%2C%22ISSN%22%3A%221664-302X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.frontiersin.org%5C%2Farticle%5C%2F10.3389%5C%2Ffmicb.2015.00552%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A38%3A45Z%22%7D%7D%2C%7B%22key%22%3A%22RNX7YUMW%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222014-08-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20RJ%2C%20David%20Prangishvili%2C%20and%20Mart%20Krupovic.%202014.%20%26%23x201C%3BHard%20out%20There%3A%20Understanding%20Archaeal%20Virus%20Biology.%26%23x201D%3B%20%3Ci%3EFuture%20Virology%3C%5C%2Fi%3E%209%20%288%29%3A%20703%26%23x2013%3B6.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.2217%5C%2Ffvl.14.52%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.2217%5C%2Ffvl.14.52%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Hard%20out%20there%3A%20understanding%20archaeal%20virus%20biology%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20RJ%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22August%201%2C%202014%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.2217%5C%2Ffvl.14.52%22%2C%22ISSN%22%3A%221746-0794%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.futuremedicine.com%5C%2Fdoi%5C%2F10.2217%5C%2Ffvl.14.52%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-20T12%3A05%3A28Z%22%7D%7D%2C%7B%22key%22%3A%22TYWKXDBK%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Krupovic%20et%20al.%22%2C%22parsedDate%22%3A%222014-02-15%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKrupovic%2C%20Mart%2C%20Emmanuelle%20R.%20J.%20Quemin%2C%20Dennis%20H.%20Bamford%2C%20Patrick%20Forterre%2C%20and%20David%20Prangishvili.%202014.%20%26%23x201C%3BUnification%20of%20the%20Globally%20Distributed%20Spindle-Shaped%20Viruses%20of%20the%20Archaea.%26%23x201D%3B%20%3Ci%3EJournal%20of%20Virology%3C%5C%2Fi%3E%2C%20February.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.02941-13%27%3Ehttps%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.02941-13%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Unification%20of%20the%20Globally%20Distributed%20Spindle-Shaped%20Viruses%20of%20the%20Archaea%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dennis%20H.%22%2C%22lastName%22%3A%22Bamford%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Forterre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%5D%2C%22abstractNote%22%3A%22Viruses%20with%20spindle-shaped%20virions%20are%20abundant%20in%20diverse%20environments.%20Over%20the%5Cnyears%2C%20such%20viruses%20have%20been%20isolated%20from%20a%20wide%20range%20of%20archaeal%20hosts.%20Evolutionary%5Cnrelationships%20between%20them%20remained%20enigmatic%2C%20however.%20Here%2C%20using%20...%22%2C%22date%22%3A%222014-2-15%22%2C%22language%22%3A%22EN%22%2C%22DOI%22%3A%22%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.02941-13%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A40%3A33Z%22%7D%7D%2C%7B%22key%22%3A%22Y77LI7NH%22%2C%22library%22%3A%7B%22id%22%3A3888256%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Quemin%20et%20al.%22%2C%22parsedDate%22%3A%222013-12-15%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EQuemin%2C%20Emmanuelle%20R.%20J.%2C%20Soizick%20Lucas%2C%20Bertram%20Daum%2C%20Tessa%20E.%20F.%20Quax%2C%20Werner%20K%26%23xFC%3Bhlbrandt%2C%20Patrick%20Forterre%2C%20Sonja-Verena%20Albers%2C%20David%20Prangishvili%2C%20and%20Mart%20Krupovic.%202013.%20%26%23x201C%3BFirst%20Insights%20into%20the%20Entry%20Process%20of%20Hyperthermophilic%20Archaeal%20Viruses.%26%23x201D%3B%20%3Ci%3EJournal%20of%20Virology%3C%5C%2Fi%3E%2C%20December.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.02742-13%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1128%5C%2FJVI.02742-13%3C%5C%2Fa%3E.%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22First%20Insights%20into%20the%20Entry%20Process%20of%20Hyperthermophilic%20Archaeal%20Viruses%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%20R.%20J.%22%2C%22lastName%22%3A%22Quemin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Soizick%22%2C%22lastName%22%3A%22Lucas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertram%22%2C%22lastName%22%3A%22Daum%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tessa%20E.%20F.%22%2C%22lastName%22%3A%22Quax%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Werner%22%2C%22lastName%22%3A%22K%5Cu00fchlbrandt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%22%2C%22lastName%22%3A%22Forterre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonja-Verena%22%2C%22lastName%22%3A%22Albers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Prangishvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mart%22%2C%22lastName%22%3A%22Krupovic%22%7D%5D%2C%22abstractNote%22%3A%22A%20decisive%20step%20in%20a%20virus%20infection%20cycle%20is%20the%20recognition%20of%20a%20specific%20receptor%5Cnpresent%20on%20the%20host%20cell%20surface%2C%20subsequently%20leading%20to%20the%20delivery%20of%20the%20viral%5Cngenome%20into%20the%20cell%20interior.%20Until%20now%2C%20the%20early%20stages%20of%20infection%20have%20...%22%2C%22date%22%3A%222013-12-15%22%2C%22language%22%3A%22EN%22%2C%22DOI%22%3A%2210.1128%5C%2FJVI.02742-13%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.asm.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1128%5C%2FJVI.02742-13%22%2C%22collections%22%3A%5B%22R7I3GKDL%22%5D%2C%22dateModified%22%3A%222021-12-16T13%3A43%3A20Z%22%7D%7D%5D%7D
Hofstadter, William A., Katelyn C. Cook, Elene Tsopurashvili, Robert Gebauer, Vojtěch Pražák, Emily A. Machala, Ji Woo Park, Kay Grünewald, Emmanuelle R. J. Quemin, and Ileana M. Cristea. 2024. “Infection-Induced Peripheral Mitochondria Fission Drives ER Encapsulations and Inter-Mitochondria Contacts That Rescue Bioenergetics.” Nature Communications 15 (1): 7352. https://doi.org/10.1038/s41467-024-51680-4.
Williams, Harry M., Sigurdur R. Thorkelsson, Dominik Vogel, Carola Busch, Morlin Milewski, Stephen Cusack, Kay Grünewald, Emmanuelle R. J. Quemin, and Maria Rosenthal. 2024. “Structural Snapshots of Phenuivirus Cap-Snatching and Transcription.” Nucleic Acids Research, May, gkae330. https://doi.org/10.1093/nar/gkae330.
Liu, Jiasui, Simon Corroyer-Dulmont, Vojtěch Pražák, Iskander Khusainov, Karola Bahrami, Sonja Welsch, Daven Vasishtan, et al. 2024. “The Palisade Layer of the Poxvirus Core Is Composed of Flexible A10 Trimers.” Nature Structural & Molecular Biology, February. https://doi.org/10.1038/s41594-024-01218-5.
Williams, Harry M., Sigurdur R. Thorkelsson, Dominik Vogel, Morlin Milewski, Carola Busch, Stephen Cusack, Kay Grünewald, Emmanuelle R. J. Quemin, and Maria Rosenthal. 2023. “Structural Insights into Viral Genome Replication by the Severe Fever with Thrombocytopenia Syndrome Virus L Protein.” Nucleic Acids Research, January, gkac1249. https://doi.org/10.1093/nar/gkac1249.
Villalta, Alejandro, Alain Schmitt, Leandro F Estrozi, Emmanuelle RJ Quemin, Jean-Marie Alempic, Audrey Lartigue, Vojtěch Pražák, et al. 2022. “The Giant Mimivirus 1.2 Mb Genome Is Elegantly Organized into a 30 Nm Diameter Helical Protein Shield.” Edited by Adam Frost. ELife 11 (July):e77607. https://doi.org/10.7554/eLife.77607.
Kouba, Tomas, Dominik Vogel, Sigurdur R. Thorkelsson, Emmanuelle R. J. Quemin, Harry M. Williams, Morlin Milewski, Carola Busch, et al. 2021. “Conformational Changes in Lassa Virus L Protein Associated with Promoter Binding and RNA Synthesis Activity.” Nature Communications 12 (1): 7018. https://doi.org/10.1038/s41467-021-27305-5.
Meier, Kristina, Sigurdur R. Thorkelsson, Emmanuelle R. J. Quemin, and Maria Rosenthal. 2021. “Hantavirus Replication Cycle—An Updated Structural Virology Perspective.” Viruses 13 (8): 1561. https://doi.org/10.3390/v13081561.
Vogel, Dominik, Sigurdur Rafn Thorkelsson, Emmanuelle R J Quemin, Kristina Meier, Tomas Kouba, Nadja Gogrefe, Carola Busch, et al. 2020. “Structural and Functional Characterization of the Severe Fever with Thrombocytopenia Syndrome Virus L Protein.” Nucleic Acids Research 48 (10): 5749–65. https://doi.org/10.1093/nar/gkaa253.
Quemin, Emmanuelle R.J., Emily A. Machala, Benjamin Vollmer, Vojtěch Pražák, Daven Vasishtan, Rene Rosch, Michael Grange, Linda E. Franken, Lindsay A. Baker, and Kay Grünewald. 2020. “Cellular Electron Cryo-Tomography to Study Virus-Host Interactions.” Annual Review of Virology 7 (1): 239–62. https://doi.org/10.1146/annurev-virology-021920-115935.
Quemin, E. R., S. Corroyer-Dulmont, A. Baskaran, E. Penard, A. D. Gazi, E. Christo-Foroux, P. Walther, C. Abergel, and J. Krijnse-Locker. 2019. “Complex Membrane Remodeling during Virion Assembly of the 30,000-Year-Old Mollivirus Sibericum.” Journal of Virology, April. https://doi.org/10.1128/JVI.00388-19.
Quemin, Emmanuelle R., Simon Corroyer-Dulmont, and Jacomine Krijnse-Locker. 2018. “Entry and Disassembly of Large DNA Viruses: Electron Microscopy Leads the Way.” Journal of Molecular Biology 430 (12): 1714–24. https://doi.org/10.1016/j.jmb.2018.04.019.
Badders, Nisha M., Ane Korff, Helen C. Miranda, Pradeep K. Vuppala, Rebecca B. Smith, Brett J. Winborn, Emmanuelle R. Quemin, et al. 2018. “Selective Modulation of the Androgen Receptor AF2 Domain Rescues Degeneration in Spinal Bulbar Muscular Atrophy.” Nature Medicine 24 (4): 427–37. https://doi.org/10.1038/nm.4500.
Quemin, Emmanuelle R. J., Petr Chlanda, Martin Sachse, Patrick Forterre, David Prangishvili, and Mart Krupovic. 2016. “Eukaryotic-Like Virus Budding in Archaea.” MBio, September. https://doi.org/10.1128/mBio.01439-16.
Rensen, Elena Ilka, Tomohiro Mochizuki, Emmanuelle Quemin, Stefan Schouten, Mart Krupovic, and David Prangishvili. 2016. “A Virus of Hyperthermophilic Archaea with a Unique Architecture among DNA Viruses.” Proceedings of the National Academy of Sciences 113 (9): 2478–83. https://doi.org/10.1073/pnas.1518929113.
Quemin, Emmanuelle R. J., Maija K. Pietilä, Hanna M. Oksanen, Patrick Forterre, W. Irene C. Rijpstra, Stefan Schouten, Dennis H. Bamford, David Prangishvili, and Mart Krupovic. 2015. “Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids.” Journal of Virology, September. https://doi.org/10.1128/JVI.02270-15.
Quemin, Emmanuelle R. J., and Tessa E. F. Quax. 2015. “Archaeal Viruses at the Cell Envelope: Entry and Egress.” Frontiers in Microbiology 6:552. https://doi.org/10.3389/fmicb.2015.00552.
Quemin, Emmanuelle RJ, David Prangishvili, and Mart Krupovic. 2014. “Hard out There: Understanding Archaeal Virus Biology.” Future Virology 9 (8): 703–6. https://doi.org/10.2217/fvl.14.52.
Krupovic, Mart, Emmanuelle R. J. Quemin, Dennis H. Bamford, Patrick Forterre, and David Prangishvili. 2014. “Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea.” Journal of Virology, February. https://journals.asm.org/doi/abs/10.1128/JVI.02941-13.
Quemin, Emmanuelle R. J., Soizick Lucas, Bertram Daum, Tessa E. F. Quax, Werner Kühlbrandt, Patrick Forterre, Sonja-Verena Albers, David Prangishvili, and Mart Krupovic. 2013. “First Insights into the Entry Process of Hyperthermophilic Archaeal Viruses.” Journal of Virology, December. https://doi.org/10.1128/JVI.02742-13.
2021
Kouba, Tomas, Dominik Vogel, Sigurdur R. Thorkelsson, Emmanuelle R. J. Quemin, Harry M. Williams, Morlin Milewski, Carola Busch, et al. 2021. “Conformational Changes in Lassa Virus L Protein Associated with Promoter Binding and RNA Synthesis Activity.” Nature Communications 12 (1): 7018. https://doi.org/10.1038/s41467-021-27305-5.
Meier, Kristina, Sigurdur R. Thorkelsson, Emmanuelle R. J. Quemin, and Maria Rosenthal. 2021. “Hantavirus Replication Cycle—An Updated Structural Virology Perspective.” Viruses 13 (8): 1561. https://doi.org/10.3390/v13081561.
2020
Vogel, Dominik, Sigurdur Rafn Thorkelsson, Emmanuelle R J Quemin, Kristina Meier, Tomas Kouba, Nadja Gogrefe, Carola Busch, et al. 2020. “Structural and Functional Characterization of the Severe Fever with Thrombocytopenia Syndrome Virus L Protein.” Nucleic Acids Research 48 (10): 5749–65. https://doi.org/10.1093/nar/gkaa253.
Quemin, Emmanuelle R.J., Emily A. Machala, Benjamin Vollmer, Vojtěch Pražák, Daven Vasishtan, Rene Rosch, Michael Grange, Linda E. Franken, Lindsay A. Baker, and Kay Grünewald. 2020. “Cellular Electron Cryo-Tomography to Study Virus-Host Interactions.” Annual Review of Virology 7 (1): 239–62. https://doi.org/10.1146/annurev-virology-021920-115935.
2019
Quemin, E. R., S. Corroyer-Dulmont, A. Baskaran, E. Penard, A. D. Gazi, E. Christo-Foroux, P. Walther, C. Abergel, and J. Krijnse-Locker. 2019. “Complex Membrane Remodeling during Virion Assembly of the 30,000-Year-Old Mollivirus Sibericum.” Journal of Virology, April. https://doi.org/10.1128/JVI.00388-19.
2018
Badders, Nisha M., Ane Korff, Helen C. Miranda, Pradeep K. Vuppala, Rebecca B. Smith, Brett J. Winborn, Emmanuelle R. Quemin, et al. 2018. “Selective Modulation of the Androgen Receptor AF2 Domain Rescues Degeneration in Spinal Bulbar Muscular Atrophy.” Nature Medicine 24 (4): 427–37. https://doi.org/10.1038/nm.4500.
Quemin, Emmanuelle R., Simon Corroyer-Dulmont, and Jacomine Krijnse-Locker. 2018. “Entry and Disassembly of Large DNA Viruses: Electron Microscopy Leads the Way.” Journal of Molecular Biology 430 (12): 1714–24. https://doi.org/10.1016/j.jmb.2018.04.019.
2016
Quemin, Emmanuelle R. J., Petr Chlanda, Martin Sachse, Patrick Forterre, David Prangishvili, and Mart Krupovic. 2016. “Eukaryotic-Like Virus Budding in Archaea.” MBio, September. https://doi.org/10.1128/mBio.01439-16.
Rensen, Elena Ilka, Tomohiro Mochizuki, Emmanuelle Quemin, Stefan Schouten, Mart Krupovic, and David Prangishvili. 2016. “A Virus of Hyperthermophilic Archaea with a Unique Architecture among DNA Viruses.” Proceedings of the National Academy of Sciences 113 (9): 2478–83. https://doi.org/10.1073/pnas.1518929113.
2015
Quemin, Emmanuelle R. J., Maija K. Pietilä, Hanna M. Oksanen, Patrick Forterre, W. Irene C. Rijpstra, Stefan Schouten, Dennis H. Bamford, David Prangishvili, and Mart Krupovic. 2015. “Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids.” Journal of Virology, September. https://doi.org/10.1128/JVI.02270-15.
Quemin, Emmanuelle R. J., and Tessa E. F. Quax. 2015. “Archaeal Viruses at the Cell Envelope: Entry and Egress.” Frontiers in Microbiology 6: 552. https://doi.org/10.3389/fmicb.2015.00552.
2014
Krupovic, Mart, Emmanuelle R. J. Quemin, Dennis H. Bamford, Patrick Forterre, and David Prangishvili. 2014. “Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea.” Journal of Virology, February. https://journals.asm.org/doi/abs/10.1128/JVI.02941-13.
Quemin, Emmanuelle RJ, David Prangishvili, and Mart Krupovic. 2014. “Hard out There: Understanding Archaeal Virus Biology.” Future Virology 9 (8): 703–6. https://doi.org/10.2217/fvl.14.52.
2013
Quemin, Emmanuelle R. J., Soizick Lucas, Bertram Daum, Tessa E. F. Quax, Werner Kühlbrandt, Patrick Forterre, Sonja-Verena Albers, David Prangishvili, and Mart Krupovic. 2013. “First Insights into the Entry Process of Hyperthermophilic Archaeal Viruses.” Journal of Virology, December. https://doi.org/10.1128/JVI.02742-13.