Fluorescent biosensors are molecules which, once introduced into living cells, yield a specific biological signal that can be measured by imaging or cytometry. The sensitivity and high throughput of flow cytometry allow the development of a whole panel of additional analyzes essential to understanding the observed biological process. The differences in physiological states observed in imaging can then be quantified in cytometry. Moreover, cytometry allows multiparametric analyzes offering the possibility of measuring several activities simultaneously cell by cell. It is thus easy to compare the results obtained, to deduce their interdependence or not, which makes it an essential tool for the study of cell signaling.
See also Biosensors Imaging in Light Microscopy section