Cytometry

Equipments

This Cytoflex is equipped with a 405-nm laser with 4 fluorescence detectors, a 488-nm laser with 2 fluorescence detectors, a 561-nm laser with 4 fluorescence detectors and a 640-nm laser with 3 fluorescence detectors, in addition to Forward- and Side-scatter detectors. Fluorescence detectors are avalanche photodiodes (APD), much more sensitive than photomultipliers (PMT). It is equipped with a plate reader and allows an absolute volumetric counting.

The optical diagram of the machine will help you choose your fluorochromes.

This modern, economical, user-friendly and intuitive cytometer is dedicated to autonomous users. Training by the engineer in charge makes it possible to understand the functionalities in a few hours.

The optical diagram of the machine will help you choose your fluorochromes.

This flow cytometer provides the technology and the power necessary for the simultaneous reading of more than 20 fluorescence parameters and for the sorting of events, even if they are rare, in a population of heterogeneous objects. It is equipped with 22 photomultipliers, and 5 laser lines (355 nm / 100 mW; 405 nm / 55 mW; 488 nm / 150 mW; 561 nm / 200 mW; 640 nm / 100mW). Sorting can be accomplished in six ways (harvest in 5ml or eppendorf tubes) or on a plate (6 to more than 96 wells) with a reading speed of 70,000 events per second. Since January 2015, the analyzer-sorter has benefited from a module allowing the detection of small events (≥200 nm).

Although it is difficult to train for sorting with occasional use, training in autonomy in analysis is possible and recommended for routine experiments.

The optical scheme of the machine will help you choose your fluorochromes.

Available software

Analysis station with the following software:

 

  • FlowLogic 7.2 (Miltenyi-Biotech / Inivai)

For the analysis of flow cytometry data with 3 modules:
Analysis (gateLogic): overlays, heat maps, cell cycle, kinetics, cell proliferation
Statistics (graphLogic): statistical analyzes and creation of graphs
Report (docLogic): layout, pdf generation

  • Kaluza Analysis Software (Beckman Coulter)

Complete cytometry software, with the possibility of exporting fcs data to csv

  • CytExpert 2.2 (Beckman Coulter)

Basic and free software that controls the cytoflex

  • Summit 6.3 (Beckman Coulter)

Basic and free software that controls the sorter

  • R and Bioconductor tools (3.6)

Services

To define the type of device most appropriate to your problem and the desired level of access, a first discussion with one of the members of the platform is recommended.

Three levels of access are offered to our users:

The provision of assisted service concerns work carried out by the platform on behalf of a user. It implements techniques and know-how mastered by the platform. The work done in this type of service is not inventive.

An analysis of the results obtained will be provided in PDF format. It implies recognition of the engineer’s investment in possible publications. The platform does not guarantee the retention of data at the end of the service.

The use of equipment requires prior user training by one of the platform members. This training results in the acquisition of autonomy allowing the user to reserve the equipment on which he has been trained.

Use of the equipment after training is suggested when the user’s needs require repeated use of the equipment.

Before being fully autonomous on the machines, the user must agree to comply with the platform access conditions by completing an “access request”. The user’s team leader must also agree by signing this document.

The platform must be thanked in any publications.

In addition to the training provided as part of our service offers to lead to the autonomy of use of equipment, Imagerie-Gif organizes training in the form of theoretical courses, practical work and tutorials in specific areas of cytometry : link to training page.

The purpose of scientific collaboration is to remove a methodological barrier, in direct collaboration with a user. Collaboration is particularly implemented when a user’s request requires adapting protocols or carrying out specific developments.

The engineer involved will be considered in the position of co-authors in a possible publication.

Acknowledgments

Funding is essential for the proper functioning and development of the Imagerie-Gif facilities. To this end, it is essential to acknowledge the facility as soon as you have benefited from the help of the engineers or any equipment.

Use this sentence to acknowledge the Flow Cytometry Core Facility :

The present work has benefited from Imagerie‐Gif core facility supported by l’Agence Nationale de la Recherche (ANR-11-EQPX-0029/Morphoscope, ANR-10-INBS-04/FranceBioImaging ; ANR‐11‐IDEX‐0003‐02/ Saclay Plant Sciences )

Use this address for the Light Microscopy Core Facility :

Flow Cytometry Facility, Imagerie-Gif, Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.

Rates

Contact

Plateforme de Microscopie Photonique / Light Microscopy Facility
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
1 Rue de la Terrasse

91198, Gif-sur-Yvette, France.

 

Le Bars Romain, Head of facility, Researcher Engineer

Mail : romain.lebars@i2bc.paris-saclay.fr

Phone: +33 1 69 82 46 40

 

Lecart Sandrine, Researcher Engineer

Mail : sandrine.lecart@i2bc.paris-saclay.fr

Phone: +33 1 69 82 46 40

 

Temagoult Mébarek, Engineer

Mail : mebarek.temagoult@i2bc.paris-saclay.fr

Phone: +33 1 69 82 46 40

 

Training

Training soon
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