Super-resolution microscopy is a family of technics (PALM, SIM, STED…) allowing to break the diffraction barrier that limits the resolution of conventional microscopes around 250nm. Although these techniques are very powerful (XY resolution is increased up to 10 times), they are nevertheless very complex to implement. The facility offers the possibility to perform PALM/dSTORM, Lattice SIM and Live-SR depending of the question and the limitations of the sample. Engineers have developed methods and tools to assist the users in the sample preparation, image acquisition, image reconstruction and quantification.
Live-SR is a module implemented on a spinning disk, allowing to increase the XY resolution up to 150 nm without any limitation concerning the fluorescent tags used, the frame rate or the sample preparation.